Restriction map for
pFN21AE4060
(Circular) (Six-base) MAPSORT of: pFN21AE4060.seq Check: 2318 from: 1 to: 5707
pFN21AE4060 5707 bp
With 182 enzymes: SgfI *
March 28, 2012 13:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3006 3834 4207 1563
Size: 1443 828 373 3063
Fragments arranged by size:
3063 1443 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2397 2600 3770 5154 5422 1632
Size: 765 203 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1170 765 268 203
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 2143 2304 4436 2143
Size: 161 2132 3414
Fragments arranged by size:
3414 2132 161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2161 2855 3450 5022 2161
Size: 694 595 1572 2846
Fragments arranged by size:
2846 1572 694 595
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 5707
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2483 2998 3016 3242 3394 5503 1493
Size: 990 515 18 226 152 2109 1697
Fragments arranged by size:
2109 1697 990 515 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 2245 3037 3404 2245
Size: 792 367 4548
Fragments arranged by size:
4548 792 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2265 3026 1391
Size: 627 247 761 4072
Fragments arranged by size:
4072 761 627 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 2890 928
Size: 971 223 768 3745
Fragments arranged by size:
3745 971 768 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BfrBI ATG'CAT
Cuts at: 2769 2769
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2053 2065 4318
Size: 107 122 71 631 605 381 12 2253
Cuts at: 4318 136
Size: 1525
Fragments arranged by size:
2253 1525 631 605 381 122 107 71
12
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2018 3768 4924 1790
Size: 228 1750 1156 2573
Fragments arranged by size:
2573 1750 1156 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 4367 1239
Size: 944 2184 2579
Fragments arranged by size:
2579 2184 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2756 2948 3832 4697 4995 2756
Size: 192 884 865 298 3468
Fragments arranged by size:
3468 884 865 298 192
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 2244 5701 2244
Size: 3457 2250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2162 2171 2192 1178
Size: 30 165 30 738 21 9 21 4693
Fragments arranged by size:
4693 738 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2062 2206 4910 5406 2062
Size: 144 2704 496 2363
Fragments arranged by size:
2704 2363 496 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 3768 3853 4924 2265
Size: 1503 85 1071 3048
Fragments arranged by size:
3048 1503 1071 85
BsmI GAATG_Cn'
Cuts at: 2463 2629 3218 3311 2463
Size: 166 589 93 4859
Fragments arranged by size:
4859 589 166 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2966 2966
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2265 3026 3768 3853 4924 1391
Size: 399 228 247 761 742 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 399 247 228 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 2354 2606 3062 844
Size: 1397 113 252 456 3489
Fragments arranged by size:
3489 1397 456 252 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2793 4202 4376 65
Size: 1183 1545 1409 174 1396
Fragments arranged by size:
1545 1409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3097
BssHII G'CGCG_C
Cuts at: 1925 2097 1925
Size: 172 5535
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2158 2516 2673 794
Size: 1364 358 157 3828
Fragments arranged by size:
3828 1364 358 157
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2239 2245 3037 3404 3789 3806 5258 5344
Size: 267 6 792 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 792 385 367 346 267 86
17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 2004 2051 2400 513
Size: 552 939 47 349 3820
Fragments arranged by size:
3820 939 552 349 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 3223 4017 4037 2848
Size: 375 794 20 4518
Fragments arranged by size:
4518 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672
Size: 54 1425 50 275 18 1324 889 1629
Cuts at: 5672 8
Size: 43
Fragments arranged by size:
1629 1425 1324 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2363 3637 1353
Size: 1010 1274 3423
Fragments arranged by size:
3423 1274 1010
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2263 2263
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2397 2600 3770 4367 5154 5422
Size: 393 551 214 203 1170 597 787 268
Cuts at: 5422 1239
Size: 1524
Fragments arranged by size:
1524 1170 787 597 551 393 268 214
203
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 5707
EcoO109I rG'GnC_Cy
Cuts at: 2210 2210
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FspI TGC'GCA
Cuts at: 2315 4213 2315
Size: 1898 3809
HaeII r_GCGC'y
Cuts at: 1678 2322 4854 1678
Size: 644 2532 2531
Fragments arranged by size:
2532 2531 644
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2162 2194 2290
Size: 32 135 32 557 32 1 32 96
Cuts at: 2290 2322 4348 4380 4422 4454 1373
Size: 32 2026 32 42 32 2626
Fragments arranged by size:
2626 2026 557 135 96 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2386 4821 5005 1305
Size: 822 259 2435 184 2007
Fragments arranged by size:
2435 2007 822 259 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2764 2774 3665 4024 4183 518
Size: 648 1598 10 891 359 159 2042
Fragments arranged by size:
2042 1598 891 648 359 159 10
MspA1I CmG'CkG
Cuts at: 1606 2032 2062 2402 3123 3800 4948 5193 1606
Size: 426 30 340 721 677 1148 245 2120
Fragments arranged by size:
2120 1148 721 677 426 340 245 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NsiI A_TGCA'T
Cuts at: 2771 2771
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2092 1348
Size: 744 4963
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2196 3059 830
Size: 1366 863 3478
Fragments arranged by size:
3478 1366 863
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
SacI G_AGCT'C
Cuts at: 2265 2265
Size: 5707
SacII CC_GC'GG
Cuts at: 2403 2403
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 2196 3059 2196
Size: 863 4844
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2192 3055 4190 4871 5062 826
Size: 219 1147 863 1135 681 191 1471
Fragments arranged by size:
1471 1147 1135 863 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2735 2963 3811 4712 4974 820
Size: 197 926 792 228 848 901 262 1553
Fragments arranged by size:
1553 926 901 848 792 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 2989 3631 5
Size: 47 2937 642 2081
Fragments arranged by size:
2937 2081 642 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 3146 513
Size: 552 939 1142 3074
Fragments arranged by size:
3074 1142 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 3953 5665
Size: 274 80 33 51 1443 729 1247 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1247 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 5667 5699 2237
Size: 32 3398 32 2245
Fragments arranged by size:
3398 2245 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI