Restriction map for
pFN21AE4059
(Circular) (Six-base) MAPSORT of: pFN21AE4059.seq Check: 2540 from: 1 to: 5686
pFN21AE4059 5686 bp
With 182 enzymes: SgfI *
March 28, 2012 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2100 2192 2938 2100
Size: 92 746 4848
Fragments arranged by size:
4848 746 92
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 2119 3007 2119
Size: 888 4798
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 3749 5133 5401 1632
Size: 470 1647 1384 268 1917
Fragments arranged by size:
1917 1647 1384 470 268
AfeI AGC'GCT
Cuts at: 2655 2655
Size: 5686
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 2634 4585 5562 1203
Size: 1431 1951 977 1327
Fragments arranged by size:
1951 1431 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2213 2817 1184
Size: 1029 604 4053
Fragments arranged by size:
4053 1029 604
AlwNI CAG_nnn'CTG
Cuts at: 2743 3429 5001 2743
Size: 686 1572 3428
Fragments arranged by size:
3428 1572 686
ApaI G_GGCC'C
Cuts at: 2298 2298
Size: 5686
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 2287 2578 3007 4462
Size: 325 217 627 332 168 291 429 1455
Cuts at: 4462 618
Size: 1842
Fragments arranged by size:
1842 1455 627 429 332 325 291 217
168
BanII G_rGCy'C
Cuts at: 1391 2298 3005 1391
Size: 907 707 4072
Fragments arranged by size:
4072 907 707
BbeI G_GCGC'C
Cuts at: 2291 2582 2291
Size: 291 5395
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BbvCI CC'TCA_GC
Cuts at: 2574 2574
Size: 5686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2619 2653 3877 3911 1903
Size: 34 682 34 1224 34 3678
Fragments arranged by size:
3678 1224 682 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 2880 1548
Size: 1332 4354
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 2298 2806 2837 3747 4903 1790
Size: 508 508 31 910 1156 2573
Fragments arranged by size:
2573 1156 910 508 508 31
BmgBI CAC'GTC
Cuts at: 1318 2637 1318
Size: 1319 4367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 3417 4375
Size: 416 53 83 399 24 946 1054 958
Cuts at: 4375 442
Size: 1753
Fragments arranged by size:
1753 1054 958 946 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2564 4346 1239
Size: 857 468 1782 2579
Fragments arranged by size:
2579 1782 857 468
Bpu10I CC'TnA_GC
Cuts at: 2574 2849 2574
Size: 275 5411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2642 3811 4676 4974 2213
Size: 429 1169 865 298 2925
Fragments arranged by size:
2925 1169 865 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2519 2594 4349 882
Size: 617 1020 75 1755 2219
Fragments arranged by size:
2219 1755 1020 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2288 2579 3493 3875
Size: 53 83 186 936 755 291 914 382
Cuts at: 3875 4510 275
Size: 635 1451
Fragments arranged by size:
1451 936 914 755 635 382 291 186
83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2318 2561 1186
Size: 898 234 243 4311
Fragments arranged by size:
4311 898 243 234
BseYI C'CCAG_C
Cuts at: 2014 2315 2356 2410 2438 2622 4889 5385 2014
Size: 301 41 54 28 184 2267 496 2315
Fragments arranged by size:
2315 2267 496 301 184 54 41 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2551 2813 1163
Size: 573 815 262 4036
Fragments arranged by size:
4036 815 573 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3747 3832 4903 3747
Size: 85 1071 4530
Fragments arranged by size:
4530 1071 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2806 2837 3005 3747 3832 4903
Size: 399 508 508 31 168 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 508 508 399 168 85
31
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 2192 2415 2724 2910 2938 3041 844
Size: 1256 92 223 309 186 28 103 3489
Fragments arranged by size:
3489 1256 309 223 186 103 92 28
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 2781 3573 1394
Size: 429 224 734 792 3507
Fragments arranged by size:
3507 792 734 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 4181 4355 65
Size: 1183 1419 1514 174 1396
Fragments arranged by size:
1514 1419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 2500 2707 1184
Size: 1316 207 4163
Fragments arranged by size:
4163 1316 207
BstYI r'GATC_y
Cuts at: 1972 2451 2514 3016 3383 3768 3785 5237 5323
Size: 479 63 502 367 385 17 1452 86
Cuts at: 5323 5335 5681 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 502 479 385 367 346 86
63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2202 2202
Size: 5686
BtgI C'CryG_G
Cuts at: 513 1065 2004 2175 2271 2496 2826 513
Size: 552 939 171 96 225 330 3373
Fragments arranged by size:
3373 939 552 330 225 171 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2347 3202 3996 4016 2347
Size: 855 794 20 4017
Fragments arranged by size:
4017 855 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DraIII CAC_nnn'GTG
Cuts at: 2461 2461
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2639 4693 809
Size: 507 1323 2054 1802
Fragments arranged by size:
2054 1802 1323 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2127 2178
Size: 54 1425 50 275 18 177 120 51
Cuts at: 2178 2478 3133 4022 5651 8
Size: 300 655 889 1629 43
Fragments arranged by size:
1629 1425 889 655 300 275 177 120
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3616 1353
Size: 2263 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2671 2765 2882 4273 4647 4793
Size: 1064 138 1082 94 117 1391 374 146
Cuts at: 4793 387
Size: 1280
Fragments arranged by size:
1391 1280 1082 1064 374 146 138 117
94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2102 2564 3749 4346 5133 5401
Size: 393 464 6 462 1185 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 1185 787 597 464 462 393 268
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2328 2861 2328
Size: 533 5153
EcoO109I rG'GnC_Cy
Cuts at: 2294 2321 2866 2294
Size: 27 545 5114
Fragments arranged by size:
5114 545 27
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 5686
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2291 2582 2657 2815 4833 1678
Size: 613 291 75 158 2018 2531
Fragments arranged by size:
2531 2018 613 291 158 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2518 2550 4327 4359 4401
Size: 32 135 32 946 32 1777 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 1777 946 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 3030 3276 669
Size: 988 281 232 860 246 3079
Fragments arranged by size:
3079 988 860 281 246 232
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KasI G'GCGC_C
Cuts at: 2287 2578 2287
Size: 291 5395
KpnI G_GTAC'C
Cuts at: 2123 3011 2123
Size: 888 4798
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 10
Size: 54 1425 343 348 3516
Fragments arranged by size:
3516 1425 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 4003 4162 518
Size: 648 2478 359 159 2042
Fragments arranged by size:
2478 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2360 2704 3102 3779 4927 5172 1606
Size: 754 344 398 677 1148 245 2120
Fragments arranged by size:
2120 1148 754 677 398 344 245
NarI GG'CG_CC
Cuts at: 2288 2579 2288
Size: 291 5395
NcoI C'CATG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 3373
Fragments arranged by size:
3373 939 822 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2630 2723 3044 4589 1132
Size: 97 1401 93 321 1545 2229
Fragments arranged by size:
2229 1545 1401 321 97 93
PasI CC'CwG_GG
Cuts at: 1348 2942 1348
Size: 1594 4092
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2461 2827 1108
Size: 1353 366 3967
Fragments arranged by size:
3967 1353 366
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2866 2866
Size: 5686
PshAI GACnn'nnGTC
Cuts at: 1613 2237 1613
Size: 624 5062
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspOMI G'GGCC_C
Cuts at: 2294 2294
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2769 2907 3038 830
Size: 1939 138 131 3478
Fragments arranged by size:
3478 1939 138 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2360 2360
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 2564 3934 5646 2564
Size: 1370 1712 2604
Fragments arranged by size:
2604 1712 1370
SfcI C'TryA_G
Cuts at: 826 1045 2361 2765 2903 3034 4169 4850 5041
Size: 219 1316 404 138 131 1135 681 191
Cuts at: 5041 826
Size: 1471
Fragments arranged by size:
1471 1316 1135 681 404 219 191 138
131
SfoI GGC'GCC
Cuts at: 2289 2580 2289
Size: 291 5395
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2657 3790 4691 4953 820
Size: 197 926 285 429 1133 901 262 1553
Fragments arranged by size:
1553 1133 926 901 429 285 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 2630 3044 1132
Size: 1498 414 3774
Fragments arranged by size:
3774 1498 414
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 1517
Size: 1281 4405
StyI C'CwwG_G
Cuts at: 513 1065 2004 2826 3125 513
Size: 552 939 822 299 3074
Fragments arranged by size:
3074 939 822 552 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 2629 3713 3730 3883 4068 4513
Size: 1255 669 380 1084 17 153 185 445
Cuts at: 4513 325
Size: 1498
Fragments arranged by size:
1498 1255 1084 669 445 380 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3932 5644
Size: 274 80 33 51 1443 585 1370 1712
Cuts at: 5644 96
Size: 138
Fragments arranged by size:
1712 1443 1370 585 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2320 2352 5646 5678 2320
Size: 32 3294 32 2328
Fragments arranged by size:
3294 2328 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 2105 3815 2105
Size: 1710 3976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI