Restriction map for
pFN21AE4055
(Circular) (Six-base) MAPSORT of: pFN21AE4055.seq Check: 1638 from: 1 to: 7366
pFN21AE4055 7366 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5176 278
Size: 53 83 186 936 3640 2468
Fragments arranged by size:
3640 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2036 2319 3381 4709 1937
Size: 99 283 1062 1328 4594
Fragments arranged by size:
4594 1328 1062 283 99
Acc65I G'GTAC_C
Cuts at: 2270 4687 2270
Size: 2417 4949
AclI AA'CG_TT
Cuts at: 1563 3114 3240 5493 5866 1563
Size: 1551 126 2253 373 3063
Fragments arranged by size:
3063 2253 1551 373 126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2916 2984 3152 4205 4448 4656 5429
Size: 749 535 68 168 1053 243 208 773
Cuts at: 5429 6813 7081 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1053 773 749 535 268 243
208 168 68
AflII C'TTAA_G
Cuts at: 820 1017 2816 820
Size: 197 1799 5370
Fragments arranged by size:
5370 1799 197
AflIII A'CryG_T
Cuts at: 1203 6265 7242 1203
Size: 5062 977 1327
Fragments arranged by size:
5062 1327 977
AgeI A'CCGG_T
Cuts at: 6197 6197
Size: 7366
AhdI GACnn_n'nnGTC
Cuts at: 6095 6095
Size: 7366
AleI CACnn'nnGTG
Cuts at: 1166 2965 1166
Size: 1799 5567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4140 1184
Size: 2956 4410
AlwNI CAG_nnn'CTG
Cuts at: 2894 5109 6681 2894
Size: 2215 1572 3579
Fragments arranged by size:
3579 2215 1572
ApaLI G'TGCA_C
Cuts at: 5423 6579 5423
Size: 1156 6210
ApoI r'AATT_y
Cuts at: 1493 2060 2832 3428 3581 4675 4901 5053 7162
Size: 567 772 596 153 1094 226 152 2109
Cuts at: 7162 1493
Size: 1697
Fragments arranged by size:
2109 1697 1094 772 596 567 226 153
152
AseI AT'TA_AT
Cuts at: 160 1032 5920 160
Size: 872 4888 1606
Fragments arranged by size:
4888 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
AvaI C'yCGr_G
Cuts at: 1870 1943 3123 4691 1870
Size: 73 1180 1568 4545
Fragments arranged by size:
4545 1568 1180 73
BamHI G'GATC_C
Cuts at: 4696 5063 4696
Size: 367 6999
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 4687 6142 618
Size: 325 217 627 483 2417 1455 1842
Fragments arranged by size:
2417 1842 1455 627 483 325 217
BanII G_rGCy'C
Cuts at: 1391 2194 2864 4685 1391
Size: 803 670 1821 4072
Fragments arranged by size:
4072 1821 803 670
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3671 4293 4352 928
Size: 971 1772 622 59 3942
Fragments arranged by size:
3942 1772 971 622 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5557 5591 1903
Size: 34 3620 34 3678
Fragments arranged by size:
3678 3620 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2138 5258 6468 2138
Size: 3120 1210 3036
Fragments arranged by size:
3120 3036 1210
BclI T'GATC_A
Cuts at: 1548 2065 3412 3721 3952 1548
Size: 517 1347 309 231 4962
Fragments arranged by size:
4962 1347 517 309 231
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5977 136
Size: 107 122 71 631 605 4305 1525
Fragments arranged by size:
4305 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3826 7361 3826
Size: 3535 3831
BlpI GC'TnA_GC
Cuts at: 4783 4783
Size: 7366
Bme1580I G_kGCm'C
Cuts at: 1790 5427 6583 1790
Size: 3637 1156 2573
Fragments arranged by size:
3637 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5097 6055 442
Size: 416 53 83 399 24 3680 958 1753
Fragments arranged by size:
3680 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3654 6026 1239
Size: 2415 2372 2579
Fragments arranged by size:
2579 2415 2372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2178 3264 4278 5491 6356 6654 2178
Size: 1086 1014 1213 865 298 2890
Fragments arranged by size:
2890 1213 1086 1014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6029 882
Size: 617 4530 2219
Fragments arranged by size:
4530 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3385 5157 493
Size: 711 2181 1772 2702
Fragments arranged by size:
2702 2181 1772 711
BsaBI GATnn'nnATC
Cuts at: 3546 3720 7360 3546
Size: 174 3640 3552
Fragments arranged by size:
3640 3552 174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5173 5555 6190 275
Size: 53 83 186 936 3640 382 635 1451
Fragments arranged by size:
3640 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5799 6197 6471 6618 7029 5799
Size: 398 274 147 411 6136
Fragments arranged by size:
6136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2851 2881 3024 3054 1178
Size: 30 165 30 1448 30 143 30 5490
Fragments arranged by size:
5490 1448 165 143 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7366
BseYI C'CCAG_C
Cuts at: 4305 6569 7065 4305
Size: 2264 496 4606
Fragments arranged by size:
4606 2264 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6793
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3288 4816 5577 6605 1540
Size: 275 186 1287 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2194 3763 5427 5512 6583 2194
Size: 1569 1664 85 1071 2977
Fragments arranged by size:
2977 1664 1569 1071 85
BsmI GAATG_Cn'
Cuts at: 3558 3804 4877 4970 3558
Size: 246 1073 93 5954
Fragments arranged by size:
5954 1073 246 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4481 4481
Size: 7366
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2194 2864 3763 4685 5427 5512 6583
Size: 399 404 670 899 922 742 85 1071
Cuts at: 6583 1391
Size: 2174
Fragments arranged by size:
2174 1071 922 899 742 670 404 399
85
BspEI T'CCGG_A
Cuts at: 7029 7029
Size: 7366
BspHI T'CATG_A
Cuts at: 2841 5255 6985 2841
Size: 2414 1730 3222
Fragments arranged by size:
3222 2414 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4108 4721 844
Size: 3264 613 3489
Fragments arranged by size:
3489 3264 613
BsrBI CCG'CTC
Cuts at: 1394 1823 5253 1394
Size: 429 3430 3507
Fragments arranged by size:
3507 3430 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5861 6035 65
Size: 1183 4613 174 1396
Fragments arranged by size:
4613 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4038 6010 6197 1700
Size: 110 2228 1972 187 2869
Fragments arranged by size:
2869 2228 1972 187 110
BsrGI T'GTAC_A
Cuts at: 96 4015 96
Size: 3919 3447
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7366
BssSI C'ACGA_G
Cuts at: 5426 6438 5426
Size: 1012 6354
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2648 794
Size: 1854 5512
BstBI TT'CG_AA
Cuts at: 6170 6170
Size: 7366
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7366
BstXI CCAn_nnnn'nTGG
Cuts at: 4201 4201
Size: 7366
BstYI r'GATC_y
Cuts at: 1972 2154 2221 2230 3088 3826 3917 4696 5063
Size: 182 67 9 858 738 91 779 367
Cuts at: 5063 5448 5465 6917 7003 7015 7361 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 858 779 738 385 367 346
182 91 86 67 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2150 2150
Size: 7366
BtgI C'CryG_G
Cuts at: 513 1065 4512 513
Size: 552 3447 3367
Fragments arranged by size:
3447 3367 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6627
Fragments arranged by size:
6627 683 56
BtsI GCAGTG_nn'
Cuts at: 3074 3338 4146 4614 4882 5676 5696 3074
Size: 264 808 468 268 794 20 4744
Fragments arranged by size:
4744 808 794 468 268 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7331
DraI TTT'AAA
Cuts at: 2017 3027 4670 2017
Size: 1010 1643 4713
Fragments arranged by size:
4713 1643 1010
DraIII CAC_nnn'GTG
Cuts at: 3203 3203
Size: 7366
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4354 6373 809
Size: 507 3038 2019 1802
Fragments arranged by size:
3038 2019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4471 4813 5702
Size: 54 1425 50 275 18 2641 342 889
Cuts at: 5702 7331 8
Size: 1629 43
Fragments arranged by size:
2641 1629 1425 889 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4813 1537
Size: 275 3001 4090
Fragments arranged by size:
4090 3001 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2599 3695 4083 4181 4355 5296 1353
Size: 1246 1096 388 98 174 941 3423
Fragments arranged by size:
3423 1246 1096 941 388 174 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5953 6327 6473 387
Size: 1064 138 4364 374 146 1280
Fragments arranged by size:
4364 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2192 2192
Size: 7366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2916 2984 3152 3654 4205 4448
Size: 393 749 535 68 168 502 551 243
Cuts at: 4448 4656 5429 6026 6813 7081 1239
Size: 208 773 597 787 268 1524
Fragments arranged by size:
1524 787 773 749 597 551 535 502
393 268 243 208 168 68
EcoRI G'AATT_C
Cuts at: 4675 5053 4675
Size: 378 6988
EcoRV GAT'ATC
Cuts at: 3800 3800
Size: 7366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2225 2225
Size: 7366
FspI TGC'GCA
Cuts at: 5872 5872
Size: 7366
HaeII r_GCGC'y
Cuts at: 1678 6513 1678
Size: 4835 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2908 2940 3024
Size: 32 135 32 446 32 858 32 84
Cuts at: 3024 3028 3056 3060 3397 3429 3940 3972 4445
Size: 4 28 4 337 32 511 32 473
Cuts at: 4445 4477 6007 6039 6081 6113 1373
Size: 32 1530 32 42 32 2626
Fragments arranged by size:
2626 1530 858 511 473 446 337 135
84 42 32 32 32 32 32 32
32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2095 3190 4710 4956 669
Size: 988 281 157 1095 1520 246 3079
Fragments arranged by size:
3079 1520 1095 988 281 246 157
HindIII A'AGCT_T
Cuts at: 748 2849 3460 4232 748
Size: 2101 611 772 3882
Fragments arranged by size:
3882 2101 772 611
HpaI GTT'AAC
Cuts at: 3190 4956 3190
Size: 1766 5600
KpnI G_GTAC'C
Cuts at: 2274 4691 2274
Size: 2417 4949
MfeI C'AATT_G
Cuts at: 4965 4965
Size: 7366
MluI A'CGCG_T
Cuts at: 7242 7242
Size: 7366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6480 6664 1305
Size: 5175 184 2007
Fragments arranged by size:
5175 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5544
Fragments arranged by size:
5544 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2965 4517 5324 5683 5842 518
Size: 648 1799 1552 807 359 159 2042
Fragments arranged by size:
2042 1799 1552 807 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4399 4782 5459 6607 6852 1606
Size: 2793 383 677 1148 245 2120
Fragments arranged by size:
2793 2120 1148 677 383 245
NaeI GCC'GGC
Cuts at: 4040 4040
Size: 7366
NcoI C'CATG_G
Cuts at: 513 1065 4512 513
Size: 552 3447 3367
Fragments arranged by size:
3447 3367 552
NdeI CA'TA_TG
Cuts at: 387 2747 387
Size: 2360 5006
NgoMIV G'CCGG_C
Cuts at: 4038 4038
Size: 7366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7366
NotI GC'GGCC_GC
Cuts at: 4813 4813
Size: 7366
NspI r_CATG'y
Cuts at: 1132 1229 2938 3906 4724 6269 1132
Size: 97 1709 968 818 1545 2229
Fragments arranged by size:
2229 1709 1545 968 818 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7366
PciI A'CATG_T
Cuts at: 6265 6265
Size: 7366
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 2608 1108
Size: 1365 135 5866
Fragments arranged by size:
5866 1365 135
PmeI GTTT'AAAC
Cuts at: 4670 4670
Size: 7366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5435 5467 6948 6980 5435
Size: 32 1481 32 5821
Fragments arranged by size:
5821 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4514 1613
Size: 2901 4465
PsiI TTA'TAA
Cuts at: 2619 4936 2619
Size: 2317 5049
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2893 2925 2893
Size: 32 7334
PstI C_TGCA'G
Cuts at: 830 3345 4621 4718 830
Size: 2515 1276 97 3478
Fragments arranged by size:
3478 2515 1276 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7366
PvuII CAG'CTG
Cuts at: 4399 4399
Size: 7366
SacI G_AGCT'C
Cuts at: 2194 2194
Size: 7366
SalI G'TCGA_C
Cuts at: 1936 4708 1936
Size: 2772 4594
SbfI CC_TGCA'GG
Cuts at: 4718 4718
Size: 7366
ScaI AGT'ACT
Cuts at: 2238 5614 7326 2238
Size: 3376 1712 2278
Fragments arranged by size:
3376 2278 1712
SexAI A'CCwGG_T
Cuts at: 2625 3613 4245 2625
Size: 988 632 5746
Fragments arranged by size:
5746 988 632
SfcI C'TryA_G
Cuts at: 826 1045 2560 2994 3341 4584 4617 4714 5849
Size: 219 1515 434 347 1243 33 97 1135
Cuts at: 5849 6530 6721 826
Size: 681 191 1471
Fragments arranged by size:
1515 1471 1243 1135 681 434 347 219
191 97 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7366
SmaI CCC'GGG
Cuts at: 1872 4693 1872
Size: 2821 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2193 2816 3123 3279 4293 5470
Size: 197 926 250 623 307 156 1014 1177
Cuts at: 5470 6371 6633 820
Size: 901 262 1553
Fragments arranged by size:
1553 1177 1014 926 901 623 307 262
250 197 156
SnaBI TAC'GTA
Cuts at: 493 5157 493
Size: 4664 2702
SpeI A'CTAG_T
Cuts at: 152 2701 152
Size: 2549 4817
SphI G_CATG'C
Cuts at: 1132 4724 1132
Size: 3592 3774
SspI AAT'ATT
Cuts at: 5 52 2725 3872 3938 5290 5
Size: 47 2673 1147 66 1352 2081
Fragments arranged by size:
2673 2081 1352 1147 66 47
StuI AGG'CCT
Cuts at: 1517 4633 1517
Size: 3116 4250
StyI C'CwwG_G
Cuts at: 513 1065 2337 4153 4512 4651 4805 513
Size: 552 1272 1816 359 139 154 3074
Fragments arranged by size:
3074 1816 1272 552 359 154 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2990 3302 4211 4642 5393 5410 5563
Size: 1255 1410 312 909 431 751 17 153
Cuts at: 5563 5748 6193 325
Size: 185 445 1498
Fragments arranged by size:
1498 1410 1255 909 751 445 431 312
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2236 4015 4532
Size: 274 80 33 51 1443 259 1779 517
Cuts at: 4532 5612 7324 96
Size: 1080 1712 138
Fragments arranged by size:
1779 1712 1443 1080 517 274 259 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2965 2997 3964 3996 7326 7358 2965
Size: 32 967 32 3330 32 2973
Fragments arranged by size:
3330 2973 967 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3311 1316
Size: 1995 5371
XbaI T'CTAG_A
Cuts at: 2212 2303 4702 2212
Size: 91 2399 4876
Fragments arranged by size:
4876 2399 91
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2474 2474
Size: 7366
XhoI C'TCGA_G
Cuts at: 1943 3123 1943
Size: 1180 6186
XmaI C'CCGG_G
Cuts at: 1870 4691 1870
Size: 2821 4545
XmnI GAAnn'nnTTC
Cuts at: 5495 5495
Size: 7366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5174 276
Size: 53 83 186 936 3640 2468
Fragments arranged by size:
3640 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoO109I
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI