Restriction map for
pFN21AE4052
(Circular) (Six-base) MAPSORT of: pFN21AE4052.seq Check: 346 from: 1 to: 5401
pFN21AE4052 5401 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2057 2638 3211 278
Size: 53 83 186 936 521 581 573 2468
Fragments arranged by size:
2468 936 581 573 521 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 3464 4848 5116 1632
Size: 415 1417 1384 268 1917
Fragments arranged by size:
1917 1417 1384 415 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 2085 4300 5277 1203
Size: 882 2215 977 1327
Fragments arranged by size:
2215 1327 977 882
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5401
AlwNI CAG_nnn'CTG
Cuts at: 3144 4716 3144
Size: 1572 3829
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2258 2710 2936 3088 5197 1493
Size: 765 452 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 452 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 1870
Size: 73 783 4545
Fragments arranged by size:
4545 783 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 4177 618
Size: 325 217 627 935 1455 1842
Fragments arranged by size:
1842 1455 935 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2720 1391
Size: 1329 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3592 3626 1903
Size: 34 1655 34 3678
Fragments arranged by size:
3678 1655 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2818 2818
Size: 5401
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2590 3462 4618 1790
Size: 295 505 872 1156 2573
Fragments arranged by size:
2573 1156 872 505 295
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 3132 4090
Size: 416 53 83 399 24 606 1109 958
Cuts at: 4090 442
Size: 1753
Fragments arranged by size:
1753 1109 958 606 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2218 2492 4061 1239
Size: 979 274 1569 2579
Fragments arranged by size:
2579 1569 979 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2547 3526 4391 4689 2547
Size: 979 865 298 3259
Fragments arranged by size:
3259 979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4064 882
Size: 617 2565 2219
Fragments arranged by size:
2565 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 3192 493
Size: 711 882 1106 2702
Fragments arranged by size:
2702 1106 882 711
BsaBI GATnn'nnATC
Cuts at: 5395 5395
Size: 5401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2097 2635 3208
Size: 53 83 186 936 521 43 538 573
Cuts at: 3208 3590 4225 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 573 538 521 382 186
83 53 43
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2584 2614 1178
Size: 30 165 30 1181 30 3965
Fragments arranged by size:
3965 1181 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2646 1186
Size: 1460 3941
BseYI C'CCAG_C
Cuts at: 4604 5100 4604
Size: 496 4905
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4828
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3612 4640 1540
Size: 275 186 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3462 3547 4618 3462
Size: 85 1071 4245
Fragments arranged by size:
4245 1071 85
BsmI GAATG_Cn'
Cuts at: 2132 2195 2912 3005 2132
Size: 63 717 93 4528
Fragments arranged by size:
4528 717 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2590 2720 3462 3547 4618 1391
Size: 399 295 505 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 399 295 130 85
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 2202 3290 5020 2202
Size: 1088 1730 2583
Fragments arranged by size:
2583 1730 1088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 844
Size: 1912 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 4045 4232 1700
Size: 110 718 1517 187 2869
Fragments arranged by size:
2869 1517 718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5401
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2731 3098 3483 3500 4952 5038 5050 5396
Size: 759 367 385 17 1452 86 12 346
Cuts at: 5396 1972
Size: 1977
Fragments arranged by size:
1977 1452 759 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2330 2330
Size: 5401
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3910
Fragments arranged by size:
3910 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2848 3737
Size: 54 1425 50 275 18 177 841 889
Cuts at: 3737 5366 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 841 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 3331 1353
Size: 920 1058 3423
Fragments arranged by size:
3423 1058 920
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2218 2492 3464 4061 4848 5116
Size: 393 415 171 274 972 597 787 268
Cuts at: 5116 1239
Size: 1524
Fragments arranged by size:
1524 972 787 597 415 393 274 268
171
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
EcoRV GAT'ATC
Cuts at: 2188 2188
Size: 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2345 2345
Size: 5401
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 4548 1678
Size: 2870 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2584 2616 4042 4074 4116
Size: 32 135 32 1012 32 1426 32 42
Cuts at: 4116 4148 1373
Size: 32 2626
Fragments arranged by size:
2626 1426 1012 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 4515 4699 1305
Size: 993 2217 184 2007
Fragments arranged by size:
2217 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3402
Fragments arranged by size:
3402 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2431 3359 3718 3877 518
Size: 648 1265 928 359 159 2042
Fragments arranged by size:
2042 1265 928 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2817 3494 4642 4887 1606
Size: 1211 677 1148 245 2120
Fragments arranged by size:
2120 1211 1148 677 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3910
Fragments arranged by size:
3910 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PmlI CAC'GTG
Cuts at: 2086 2086
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2473 2638 1613
Size: 860 165 4376
Fragments arranged by size:
4376 860 165
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2349 2753 830
Size: 1519 404 3478
Fragments arranged by size:
3478 1519 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 2231 3649 5361 2231
Size: 1418 1712 2271
Fragments arranged by size:
2271 1712 1418
SfcI C'TryA_G
Cuts at: 826 1045 2296 2345 2749 3884 4565 4756 826
Size: 219 1251 49 404 1135 681 191 1471
Fragments arranged by size:
1471 1251 1135 681 404 219 191 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2562 3505 4406 4668 820
Size: 197 926 619 943 901 262 1553
Fragments arranged by size:
1553 943 926 901 619 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2004 2840 513
Size: 552 939 836 3074
Fragments arranged by size:
3074 939 836 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2005 3428 3445 3598 3783 4228 325
Size: 1255 425 1423 17 153 185 445 1498
Fragments arranged by size:
1498 1423 1255 445 425 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 2229 3647
Size: 274 80 33 51 1443 111 141 1418
Cuts at: 3647 5359 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1418 274 141 138 111 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2055 2636 3209 276
Size: 53 83 186 936 521 581 573 2468
Fragments arranged by size:
2468 936 581 573 521 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI