Restriction map for
pFN21AE4047
(Circular) (Six-base) MAPSORT of: pFN21AE4047.seq Check: 4953 from: 1 to: 5524
pFN21AE4047 5524 bp
With 182 enzymes: SgfI *
March 28, 2012 13:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 4971 5239 1632
Size: 1955 1384 268 1917
Fragments arranged by size:
1955 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2166 4423 5400 1203
Size: 963 2257 977 1327
Fragments arranged by size:
2257 1327 977 963
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 2416 4253 2416
Size: 1837 3687
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 2593 2764 3267 4839 2593
Size: 171 503 1572 3278
Fragments arranged by size:
3278 1572 503 171
ApaI G_GGCC'C
Cuts at: 2505 2505
Size: 5524
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2096 2177 2699 2849 1870
Size: 73 77 76 81 522 150 4545
Fragments arranged by size:
4545 522 150 81 77 76 73
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2333 2845 4300 618
Size: 325 217 627 448 98 512 1455 1842
Fragments arranged by size:
1842 1455 627 512 448 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2097 2505 2706 2843 1391
Size: 706 408 201 137 4072
Fragments arranged by size:
4072 706 408 201 137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2191 928
Size: 971 292 4261
Fragments arranged by size:
4261 971 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2498 2532 3715 3749 1903
Size: 34 561 34 1183 34 3678
Fragments arranged by size:
3678 1183 561 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2101 2110 2323
Size: 107 122 71 631 605 429 9 213
Cuts at: 2323 2489 4135 136
Size: 166 1646 1525
Fragments arranged by size:
1646 1525 631 605 429 213 166 122
107 71 9
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 2505 2564 3585 4741 1790
Size: 715 59 1021 1156 2573
Fragments arranged by size:
2573 1156 1021 715 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4184 1239
Size: 2945 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2600 3649 4514 4812 2015
Size: 585 1049 865 298 2727
Fragments arranged by size:
2727 1049 865 585 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2618 4187 882
Size: 617 1119 1569 2219
Fragments arranged by size:
2219 1569 1119 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2412 3331 3713 4348
Size: 53 83 186 936 879 919 382 635
Cuts at: 4348 275
Size: 1451
Fragments arranged by size:
1451 936 919 879 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2762 2792 1178
Size: 30 165 30 1359 30 3910
Fragments arranged by size:
3910 1359 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5524
BseYI C'CCAG_C
Cuts at: 2427 4727 5223 2427
Size: 2300 496 2728
Fragments arranged by size:
2728 2300 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2109 2174 2279 2360 1163
Size: 573 373 65 105 81 4327
Fragments arranged by size:
4327 573 373 105 81 65
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2066 2105 2218 2974 3735 4763
Size: 275 186 65 39 113 756 761 1028
Cuts at: 4763 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 756 275 186 113 65
39
BsiHKAI G_wGCw'C
Cuts at: 2255 2371 3585 3670 4741 2255
Size: 116 1214 85 1071 3038
Fragments arranged by size:
3038 1214 1071 116 85
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2255 2371 2505 2564 2706 2843
Size: 399 307 158 116 134 59 142 137
Cuts at: 2843 3585 3670 4741 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 307 158 142 137
134 116 85 59
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 2742 3411 1394
Size: 429 364 555 669 3507
Fragments arranged by size:
3507 669 555 429 364
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 2065 2104 2217 2317 4168 4355
Size: 110 243 12 39 113 100 1851 187
Cuts at: 4355 1700
Size: 2869
Fragments arranged by size:
2869 1851 243 187 113 110 100 39
12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 2537 3584 4596 2537
Size: 1047 1012 3465
Fragments arranged by size:
3465 1047 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2732 2854 3221 3606 3623 5075 5161 5173
Size: 760 122 367 385 17 1452 86 12
Cuts at: 5173 5519 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 760 385 367 346 122 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 2266 2287 2358 2382 513
Size: 552 939 237 25 21 71 24 3655
Fragments arranged by size:
3655 939 552 237 71 25 24 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 3040 3834 3854 2413
Size: 627 794 20 4083
Fragments arranged by size:
4083 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DraIII CAC_nnn'GTG
Cuts at: 2242 2383 2242
Size: 141 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2067 2102
Size: 54 1425 50 275 18 233 4 35
Cuts at: 2102 2315 2355 2584 2971 3860 5489 8
Size: 213 40 229 387 889 1629 43
Fragments arranged by size:
1629 1425 889 387 275 233 229 213
54 50 43 40 35 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2063 2102 2971 1537
Size: 275 251 39 869 4090
Fragments arranged by size:
4090 869 275 251 39
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3587 4184 4971 5239 1239
Size: 393 1955 597 787 268 1524
Fragments arranged by size:
1955 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2230 2230
Size: 5524
EcoO109I rG'GnC_Cy
Cuts at: 2431 2501 2431
Size: 70 5454
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FseI GG_CCGG'CC
Cuts at: 2069 2069
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2060 2399 2764 4671 1678
Size: 382 339 365 1907 2531
Fragments arranged by size:
2531 1907 382 365 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 2762 2794 4165
Size: 32 135 32 830 32 328 32 1371
Cuts at: 4165 4197 4239 4271 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1371 830 328 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 2166 5400 2166
Size: 3234 2290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 4638 4822 1305
Size: 801 2532 184 2007
Fragments arranged by size:
2532 2007 801 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2357 2586 10
Size: 54 1425 343 525 229 2948
Fragments arranged by size:
2948 1425 525 343 229 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3482 3841 4000 518
Size: 648 2316 359 159 2042
Fragments arranged by size:
2316 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2138 2158 2268 2289 2940 3617 4765
Size: 516 16 20 110 21 651 677 1148
Cuts at: 4765 5010 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 651 516 245 110 21
20 16
NaeI GCC'GGC
Cuts at: 2055 2067 2106 2319 2055
Size: 12 39 213 5260
Fragments arranged by size:
5260 213 39 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4033
Fragments arranged by size:
4033 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NgoMIV G'CCGG_C
Cuts at: 2053 2065 2104 2317 2053
Size: 12 39 213 5260
Fragments arranged by size:
5260 213 39 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2134 2882 4427 1132
Size: 97 905 748 1545 2229
Fragments arranged by size:
2229 1545 905 748 97
PasI CC'CwG_GG
Cuts at: 1348 2749 1348
Size: 1401 4123
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2125 2593 1108
Size: 1017 468 4039
Fragments arranged by size:
4039 1017 468
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2431
Size: 5524
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PspOMI G'GGCC_C
Cuts at: 2501 2501
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2769 2817 2876 830
Size: 1939 48 59 3478
Fragments arranged by size:
3478 1939 59 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
PvuII CAG'CTG
Cuts at: 2122 2158 2122
Size: 36 5488
SacII CC_GC'GG
Cuts at: 2269 2290 2269
Size: 21 5503
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2769 2876 2769
Size: 107 5417
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SexAI A'CCwGG_T
Cuts at: 2820 2820
Size: 5524
SfcI C'TryA_G
Cuts at: 826 1045 2343 2765 2813 2872 4007 4688 4879
Size: 219 1298 422 48 59 1135 681 191
Cuts at: 4879 826
Size: 1471
Fragments arranged by size:
1471 1298 1135 681 422 219 191 59
48
SfiI GGCCn_nnn'nGGCC
Cuts at: 2489 2489
Size: 5524
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2098 2851 1872
Size: 226 753 4545
Fragments arranged by size:
4545 753 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 2615 3628 4529 4791 820
Size: 197 926 87 585 1013 901 262 1553
Fragments arranged by size:
1553 1013 926 901 585 262 197 87
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SrfI GCCC'GGGC
Cuts at: 2098 2098
Size: 5524
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 2513 1517
Size: 996 4528
StyI C'CwwG_G
Cuts at: 513 1065 2004 2963 513
Size: 552 939 959 3074
Fragments arranged by size:
3074 959 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 3551 3568 3721 3906 4351 325
Size: 1255 771 1200 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3770 5482 96
Size: 274 80 33 51 1443 1793 1712 138
Fragments arranged by size:
1793 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2345 2345
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2096 2849 1870
Size: 226 753 4545
Fragments arranged by size:
4545 753 226
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI
SfoI SwaI