Restriction map for
pFN21AE4038
(Circular) (Six-base) MAPSORT of: pFN21AE4038.seq Check: 9887 from: 1 to: 5464
pFN21AE4038 5464 bp
With 182 enzymes: SgfI *
March 28, 2012 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2037 2532 2037
Size: 495 4969
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2619 2785 2619
Size: 166 5298
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2341 3527 4911 5179 1632
Size: 709 1186 1384 268 1917
Fragments arranged by size:
1917 1384 1186 709 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2045 1184
Size: 861 4603
AlwNI CAG_nnn'CTG
Cuts at: 2019 2158 2223 2340 3207 4779 2019
Size: 139 65 117 867 1572 2704
Fragments arranged by size:
2704 1572 867 139 117 65
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 2789 1870
Size: 73 545 301 4545
Fragments arranged by size:
4545 545 301 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2611 2644 2611
Size: 33 5431
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2510 2619 2785 4240 618
Size: 325 217 627 723 109 166 1455 1842
Fragments arranged by size:
1842 1455 723 627 325 217 166 109
BanII G_rGCy'C
Cuts at: 1391 2195 2783 1391
Size: 804 588 4072
Fragments arranged by size:
4072 804 588
BbeI G_GCGC'C
Cuts at: 2514 2514
Size: 5464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2084 928
Size: 971 185 4308
Fragments arranged by size:
4308 971 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2232 2508 4075
Size: 107 122 71 631 605 560 276 1567
Cuts at: 4075 136
Size: 1525
Fragments arranged by size:
1567 1525 631 605 560 276 122 107
71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2059 2881 2059
Size: 822 4642
Bme1580I G_kGCm'C
Cuts at: 1790 3525 4681 1790
Size: 1735 1156 2573
Fragments arranged by size:
2573 1735 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 4153 442
Size: 416 53 83 399 24 1778 958 1753
Fragments arranged by size:
1778 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 4124 1239
Size: 1088 1797 2579
Fragments arranged by size:
2579 1797 1088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2511 2647 3271 3653
Size: 53 83 186 936 978 136 624 382
Cuts at: 3653 4288 275
Size: 635 1451
Fragments arranged by size:
1451 978 936 635 624 382 186 136
83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2663 1186
Size: 1477 3987
BseYI C'CCAG_C
Cuts at: 2418 2719 4667 5163 2418
Size: 301 1948 496 2719
Fragments arranged by size:
2719 1948 496 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2035 2049 2236 2510 1163
Size: 573 299 14 187 274 4117
Fragments arranged by size:
4117 573 299 274 187 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2210 2914 3675 4703 1540
Size: 275 186 209 704 761 1028 2301
Fragments arranged by size:
2301 1028 761 704 275 209 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3525 3610 4681 2313
Size: 1212 85 1071 3096
Fragments arranged by size:
3096 1212 1071 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2313 2783 3525 3610 4681 1391
Size: 399 405 118 470 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 470 405 399 118 85
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2532 2819 844
Size: 1193 495 287 3489
Fragments arranged by size:
3489 1193 495 287
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 4108 4295 1700
Size: 110 395 1903 187 2869
Fragments arranged by size:
2869 1903 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 2270 2634 96
Size: 2174 364 2926
Fragments arranged by size:
2926 2174 364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 2228 3524 4536 2228
Size: 1296 1012 3156
Fragments arranged by size:
3156 1296 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2223 794
Size: 1429 4035
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2220 2794 3161 3546 3563 5015 5101 5113
Size: 248 574 367 385 17 1452 86 12
Cuts at: 5113 5459 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 574 385 367 346 248 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2070 2514 513
Size: 552 1005 444 3463
Fragments arranged by size:
3463 1005 552 444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3774 3794 2980
Size: 794 20 4650
Fragments arranged by size:
4650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2608 4471 809
Size: 507 1292 1863 1802
Fragments arranged by size:
1863 1802 1292 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2478 2911
Size: 54 1425 50 275 18 377 271 433
Cuts at: 2911 3800 5429 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 433 377 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2207 2911 1537
Size: 275 395 704 4090
Fragments arranged by size:
4090 704 395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 3394 1353
Size: 1232 809 3423
Fragments arranged by size:
3423 1232 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2341 3527 4124 4911 5179 1239
Size: 393 695 14 1186 597 787 268 1524
Fragments arranged by size:
1524 1186 787 695 597 393 268 14
EcoO109I rG'GnC_Cy
Cuts at: 2277 2277
Size: 5464
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2514 4611 1678
Size: 836 2097 2531
Fragments arranged by size:
2531 2097 836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2278 2310 4105
Size: 32 135 32 668 32 6 32 1795
Cuts at: 4105 4137 4179 4211 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1795 668 135 42 32 32 32
32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2163 2808 3054 669
Size: 988 281 225 645 246 3079
Fragments arranged by size:
3079 988 645 281 246 225
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KasI G'GCGC_C
Cuts at: 2510 2510
Size: 5464
KpnI G_GTAC'C
Cuts at: 2623 2789 2623
Size: 166 5298
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2533 2630 4578 4762 1305
Size: 1228 97 1948 184 2007
Fragments arranged by size:
2007 1948 1228 184 97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2480 10
Size: 54 1425 343 648 2994
Fragments arranged by size:
2994 1425 648 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2266 3422 3781 3940 518
Size: 648 1100 1156 359 159 2042
Fragments arranged by size:
2042 1156 1100 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2063 2155 2880 3557 4705 4950 1606
Size: 413 44 92 725 677 1148 245 2120
Fragments arranged by size:
2120 1148 725 677 413 245 92 44
NarI GG'CG_CC
Cuts at: 2511 2511
Size: 5464
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4912
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2277 2277
Size: 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 2468 3034 2468
Size: 566 4898
PstI C_TGCA'G
Cuts at: 830 2024 2816 830
Size: 1194 792 3478
Fragments arranged by size:
3478 1194 792
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2063 2155 2063
Size: 92 5372
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2020 2812 3947 4628 4819 826
Size: 219 975 792 1135 681 191 1471
Fragments arranged by size:
1471 1135 975 792 681 219 191
SfoI GGC'GCC
Cuts at: 2512 2512
Size: 5464
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 2605 152
Size: 2453 3011
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2142 2280 2903 513
Size: 552 1077 138 623 3074
Fragments arranged by size:
3074 1077 623 552 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 2722 3491 3508 3661 3846 4291
Size: 1255 621 521 769 17 153 185 445
Cuts at: 4291 325
Size: 1498
Fragments arranged by size:
1498 1255 769 621 521 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2270 2634 3710
Size: 274 80 33 51 1443 293 364 1076
Cuts at: 3710 5422 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1076 364 293 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI