Restriction map for
pFN21AE4033
(Circular) (Six-base) MAPSORT of: pFN21AE4033.seq Check: 4837 from: 1 to: 5431
pFN21AE4033 5431 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2461 2461
Size: 5431
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 3494 4878 5146 1632
Size: 1061 801 1384 268 1917
Fragments arranged by size:
1917 1384 1061 801 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 2671 2695 3174 4746 2671
Size: 24 479 1572 3356
Fragments arranged by size:
3356 1572 479 24
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2529 2740 2966 3118 5227 1493
Size: 1036 211 226 152 2109 1697
Fragments arranged by size:
2109 1697 1036 226 211 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2252 2756 1870
Size: 73 108 201 504 4545
Fragments arranged by size:
4545 504 201 108 73
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2752 4207 618
Size: 325 217 627 318 647 1455 1842
Fragments arranged by size:
1842 1455 647 627 325 318 217
BanII G_rGCy'C
Cuts at: 1391 2750 1391
Size: 1359 4072
BbeI G_GCGC'C
Cuts at: 2109 2109
Size: 5431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3622 3656 1903
Size: 34 50 34 1601 34 3678
Fragments arranged by size:
3678 1601 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2239 3323 4533 2239
Size: 1084 1210 3137
Fragments arranged by size:
3137 1210 1084
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 4042 136
Size: 107 122 71 631 605 446 1924 1525
Fragments arranged by size:
1924 1525 631 605 446 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2015 2848 2015
Size: 833 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2031 3492 4648 1790
Size: 241 1461 1156 2573
Fragments arranged by size:
2573 1461 1156 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2735 3162 4120
Size: 416 53 83 399 24 1318 427 958
Cuts at: 4120 442
Size: 1753
Fragments arranged by size:
1753 1318 958 427 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2501 1056
Size: 1445 3986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4091 1239
Size: 2852 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4421 4719 3556
Size: 865 298 4268
Fragments arranged by size:
4268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4094 882
Size: 617 2595 2219
Fragments arranged by size:
2595 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2259 3238 3620
Size: 53 83 186 936 573 153 979 382
Cuts at: 3620 4255 275
Size: 635 1451
Fragments arranged by size:
1451 979 936 635 573 382 186 153
83 53
BsaWI w'CCGG_w
Cuts at: 2397 3864 4262 4536 4683 5094 2397
Size: 1467 398 274 147 411 2734
Fragments arranged by size:
2734 1467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 1178
Size: 30 165 30 810 30 4366
Fragments arranged by size:
4366 810 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 1186
Size: 864 4567
BseYI C'CCAG_C
Cuts at: 2101 2509 4634 5130 2101
Size: 408 2125 496 2402
Fragments arranged by size:
2402 2125 496 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 2495 2706 1163
Size: 573 597 162 211 3888
Fragments arranged by size:
3888 597 573 211 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2012 2113 2881 3642 4670 1540
Size: 275 186 11 101 768 761 1028 2301
Fragments arranged by size:
2301 1028 768 761 275 186 101 11
BsiHKAI G_wGCw'C
Cuts at: 3492 3577 4648 3492
Size: 85 1071 4275
Fragments arranged by size:
4275 1071 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
BsmBI CGTCTCn'nnnn_
Cuts at: 2671 2671
Size: 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2750 3492 3577 4648 1391
Size: 399 241 719 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 399 241 85
BspEI T'CCGG_A
Cuts at: 2397 5094 2397
Size: 2697 2734
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2461 2786 844
Size: 1617 325 3489
Fragments arranged by size:
3489 1617 325
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 2652 3318 1394
Size: 429 191 638 666 3507
Fragments arranged by size:
3507 666 638 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 2136 3926 4100 65
Size: 1183 888 1790 174 1396
Fragments arranged by size:
1790 1396 1183 888 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 4075 4262 1700
Size: 110 298 1967 187 2869
Fragments arranged by size:
2869 1967 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 794
Size: 1241 4190
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 2509
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2238 2265 2761 3128 3513 3530 4982 5068
Size: 266 27 496 367 385 17 1452 86
Cuts at: 5068 5080 5426 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 496 385 367 346 266 86
27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4879
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2321 2947 3741 3761 2321
Size: 626 794 20 3991
Fragments arranged by size:
3991 794 626 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2009 2110 2878
Size: 54 1425 50 275 18 179 101 768
Cuts at: 2878 3767 5396 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 768 275 179 101 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2009 2110 2878 1537
Size: 275 197 101 768 4090
Fragments arranged by size:
4090 768 275 197 101
EarI CTCTTCn'nnn_
Cuts at: 1353 2620 2669 3361 1353
Size: 1267 49 692 3423
Fragments arranged by size:
3423 1267 692 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4018 4392 4538 387
Size: 1064 138 2429 374 146 1280
Fragments arranged by size:
2429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2693 3494 4091 4878 5146 1239
Size: 393 1061 801 597 787 268 1524
Fragments arranged by size:
1524 1061 801 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 2282
Size: 5431
EcoO109I rG'GnC_Cy
Cuts at: 2452 2668 2452
Size: 216 5215
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 5431
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2109 4578 1678
Size: 431 2469 2531
Fragments arranged by size:
2531 2469 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2285 2317 2619
Size: 32 135 32 641 32 40 32 302
Cuts at: 2619 2651 4072 4104 4146 4178 1373
Size: 32 1421 32 42 32 2626
Fragments arranged by size:
2626 1421 641 302 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2105 2105
Size: 5431
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2401 4545 4729 1305
Size: 1096 2144 184 2007
Fragments arranged by size:
2144 2007 1096 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3389 3748 3907 518
Size: 648 1269 954 359 159 2042
Fragments arranged by size:
2042 1269 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2847 3524 4672 4917 1606
Size: 1241 677 1148 245 2120
Fragments arranged by size:
2120 1241 1148 677 245
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 5431
NarI GG'CG_CC
Cuts at: 2106 2106
Size: 5431
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4879
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 2497 1052
Size: 1445 3986
NotI GC'GGCC_GC
Cuts at: 2009 2878 2009
Size: 869 4562
NspI r_CATG'y
Cuts at: 1132 1229 2023 2465 2665 2789 4334 1132
Size: 97 794 442 200 124 1545 2229
Fragments arranged by size:
2229 1545 794 442 200 124 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PfoI T'CCnGG_A
Cuts at: 2241 2241
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2668 2452
Size: 216 5215
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2238 2783 830
Size: 1408 545 3478
Fragments arranged by size:
3478 1408 545
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SapI GCTCTTCn'nnn_
Cuts at: 2620 2620
Size: 5431
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2234 2779 3914 4595 4786 826
Size: 219 1189 545 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 545 219 191
SfoI GGC'GCC
Cuts at: 2107 2107
Size: 5431
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2254 2758 1872
Size: 382 504 4545
Fragments arranged by size:
4545 504 382
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3535 4436 4698 820
Size: 197 926 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2465 2789 1132
Size: 1333 324 3774
Fragments arranged by size:
3774 1333 324
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2079 2870 513
Size: 552 1014 791 3074
Fragments arranged by size:
3074 1014 791 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2059 3458 3475 3628 3813 4258 325
Size: 1255 479 1399 17 153 185 445 1498
Fragments arranged by size:
1498 1399 1255 479 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3677 5389 96
Size: 274 80 33 51 1443 1700 1712 138
Fragments arranged by size:
1712 1700 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5391 5423 5391
Size: 32 5399
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2252 2756 1870
Size: 382 504 4545
Fragments arranged by size:
4545 504 382
XmnI GAAnn'nnTTC
Cuts at: 3560 3560
Size: 5431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI