(Circular) (Six-base) MAPSORT of: pFN21AE4033.seq Check: 4837 from: 1 to: 5431 pFN21AE4033 5431 bp With 182 enzymes: SgfI * March 28, 2012 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2461 2461 Size: 5431 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2693 3494 4878 5146 1632 Size: 1061 801 1384 268 1917 Fragments arranged by size: 1917 1384 1061 801 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 4330 5307 1203 Size: 3127 977 1327 Fragments arranged by size: 3127 1327 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 2671 2695 3174 4746 2671 Size: 24 479 1572 3356 Fragments arranged by size: 3356 1572 479 24 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2529 2740 2966 3118 5227 1493 Size: 1036 211 226 152 2109 1697 Fragments arranged by size: 2109 1697 1036 226 211 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2252 2756 1870 Size: 73 108 201 504 4545 Fragments arranged by size: 4545 504 201 108 73 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2752 4207 618 Size: 325 217 627 318 647 1455 1842 Fragments arranged by size: 1842 1455 647 627 325 318 217 BanII G_rGCy'C Cuts at: 1391 2750 1391 Size: 1359 4072 BbeI G_GCGC'C Cuts at: 2109 2109 Size: 5431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3622 3656 1903 Size: 34 50 34 1601 34 3678 Fragments arranged by size: 3678 1601 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2239 3323 4533 2239 Size: 1084 1210 3137 Fragments arranged by size: 3137 1210 1084 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 4042 136 Size: 107 122 71 631 605 446 1924 1525 Fragments arranged by size: 1924 1525 631 605 446 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2015 2848 2015 Size: 833 4598 Bme1580I G_kGCm'C Cuts at: 1790 2031 3492 4648 1790 Size: 241 1461 1156 2573 Fragments arranged by size: 2573 1461 1156 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2735 3162 4120 Size: 416 53 83 399 24 1318 427 958 Cuts at: 4120 442 Size: 1753 Fragments arranged by size: 1753 1318 958 427 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2501 1056 Size: 1445 3986 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4091 1239 Size: 2852 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3556 4421 4719 3556 Size: 865 298 4268 Fragments arranged by size: 4268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4094 882 Size: 617 2595 2219 Fragments arranged by size: 2595 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2259 3238 3620 Size: 53 83 186 936 573 153 979 382 Cuts at: 3620 4255 275 Size: 635 1451 Fragments arranged by size: 1451 979 936 635 573 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 2397 3864 4262 4536 4683 5094 2397 Size: 1467 398 274 147 411 2734 Fragments arranged by size: 2734 1467 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 1178 Size: 30 165 30 810 30 4366 Fragments arranged by size: 4366 810 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 1186 Size: 864 4567 BseYI C'CCAG_C Cuts at: 2101 2509 4634 5130 2101 Size: 408 2125 496 2402 Fragments arranged by size: 2402 2125 496 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 2495 2706 1163 Size: 573 597 162 211 3888 Fragments arranged by size: 3888 597 573 211 162 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2012 2113 2881 3642 4670 1540 Size: 275 186 11 101 768 761 1028 2301 Fragments arranged by size: 2301 1028 768 761 275 186 101 11 BsiHKAI G_wGCw'C Cuts at: 3492 3577 4648 3492 Size: 85 1071 4275 Fragments arranged by size: 4275 1071 85 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 BsmBI CGTCTCn'nnnn_ Cuts at: 2671 2671 Size: 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2750 3492 3577 4648 1391 Size: 399 241 719 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 399 241 85 BspEI T'CCGG_A Cuts at: 2397 5094 2397 Size: 2697 2734 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2461 2786 844 Size: 1617 325 3489 Fragments arranged by size: 3489 1617 325 BsrBI CCG'CTC Cuts at: 1394 1823 2014 2652 3318 1394 Size: 429 191 638 666 3507 Fragments arranged by size: 3507 666 638 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 2136 3926 4100 65 Size: 1183 888 1790 174 1396 Fragments arranged by size: 1790 1396 1183 888 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2108 4075 4262 1700 Size: 110 298 1967 187 2869 Fragments arranged by size: 2869 1967 298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 794 Size: 1241 4190 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5431 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 5431 BstYI r'GATC_y Cuts at: 1972 2238 2265 2761 3128 3513 3530 4982 5068 Size: 266 27 496 367 385 17 1452 86 Cuts at: 5068 5080 5426 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 496 385 367 346 266 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4879 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2321 2947 3741 3761 2321 Size: 626 794 20 3991 Fragments arranged by size: 3991 794 626 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2009 2110 2878 Size: 54 1425 50 275 18 179 101 768 Cuts at: 2878 3767 5396 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 768 275 179 101 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2009 2110 2878 1537 Size: 275 197 101 768 4090 Fragments arranged by size: 4090 768 275 197 101 EarI CTCTTCn'nnn_ Cuts at: 1353 2620 2669 3361 1353 Size: 1267 49 692 3423 Fragments arranged by size: 3423 1267 692 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4018 4392 4538 387 Size: 1064 138 2429 374 146 1280 Fragments arranged by size: 2429 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2693 3494 4091 4878 5146 1239 Size: 393 1061 801 597 787 268 1524 Fragments arranged by size: 1524 1061 801 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2282 2282 Size: 5431 EcoO109I rG'GnC_Cy Cuts at: 2452 2668 2452 Size: 216 5215 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5431 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2109 4578 1678 Size: 431 2469 2531 Fragments arranged by size: 2531 2469 431 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2285 2317 2619 Size: 32 135 32 641 32 40 32 302 Cuts at: 2619 2651 4072 4104 4146 4178 1373 Size: 32 1421 32 42 32 2626 Fragments arranged by size: 2626 1421 641 302 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2105 2105 Size: 5431 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2401 4545 4729 1305 Size: 1096 2144 184 2007 Fragments arranged by size: 2144 2007 1096 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 3389 3748 3907 518 Size: 648 1269 954 359 159 2042 Fragments arranged by size: 2042 1269 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2847 3524 4672 4917 1606 Size: 1241 677 1148 245 2120 Fragments arranged by size: 2120 1241 1148 677 245 NaeI GCC'GGC Cuts at: 2110 2110 Size: 5431 NarI GG'CG_CC Cuts at: 2106 2106 Size: 5431 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4879 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NgoMIV G'CCGG_C Cuts at: 2108 2108 Size: 5431 NheI G'CTAG_C Cuts at: 1052 2497 1052 Size: 1445 3986 NotI GC'GGCC_GC Cuts at: 2009 2878 2009 Size: 869 4562 NspI r_CATG'y Cuts at: 1132 1229 2023 2465 2665 2789 4334 1132 Size: 97 794 442 200 124 1545 2229 Fragments arranged by size: 2229 1545 794 442 200 124 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PfoI T'CCnGG_A Cuts at: 2241 2241 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2668 2452 Size: 216 5215 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PstI C_TGCA'G Cuts at: 830 2238 2783 830 Size: 1408 545 3478 Fragments arranged by size: 3478 1408 545 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SapI GCTCTTCn'nnn_ Cuts at: 2620 2620 Size: 5431 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2234 2779 3914 4595 4786 826 Size: 219 1189 545 1135 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 545 219 191 SfoI GGC'GCC Cuts at: 2107 2107 Size: 5431 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2254 2758 1872 Size: 382 504 4545 Fragments arranged by size: 4545 504 382 SmlI C'TyrA_G Cuts at: 820 1017 1943 3535 4436 4698 820 Size: 197 926 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2465 2789 1132 Size: 1333 324 3774 Fragments arranged by size: 3774 1333 324 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2079 2870 513 Size: 552 1014 791 3074 Fragments arranged by size: 3074 1014 791 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2059 3458 3475 3628 3813 4258 325 Size: 1255 479 1399 17 153 185 445 1498 Fragments arranged by size: 1498 1399 1255 479 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3677 5389 96 Size: 274 80 33 51 1443 1700 1712 138 Fragments arranged by size: 1712 1700 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5391 5423 5391 Size: 32 5399 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2252 2756 1870 Size: 382 504 4545 Fragments arranged by size: 4545 504 382 XmnI GAAnn'nnTTC Cuts at: 3560 3560 Size: 5431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI