Restriction map for
pFN21AE4028
(Circular) (Six-base) MAPSORT of: pFN21AE4028.seq Check: 4903 from: 1 to: 5413
pFN21AE4028 5413 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2711 3156 4728 2711
Size: 445 1572 3396
Fragments arranged by size:
3396 1572 445
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2519 2722 2948 3100 5209 1493
Size: 1026 203 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2514 2738 1870
Size: 73 81 490 224 4545
Fragments arranged by size:
4545 490 224 81 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2424 2457 2424
Size: 33 5380
BamHI G'GATC_C
Cuts at: 2238 2743 3110 2238
Size: 505 367 4541
Fragments arranged by size:
4541 505 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 4189 618
Size: 325 217 627 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2109 2732 1391
Size: 718 623 4072
Fragments arranged by size:
4072 718 623
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 3305 4515 2272
Size: 1033 1210 3170
Fragments arranged by size:
3170 1210 1033
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 3144 4102
Size: 416 53 83 399 24 652 1075 958
Cuts at: 4102 442
Size: 1753
Fragments arranged by size:
1753 1075 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4073 1239
Size: 2834 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4403 4701 3538
Size: 865 298 4250
Fragments arranged by size:
4250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2603 3204 493
Size: 711 1399 601 2702
Fragments arranged by size:
2702 1399 711 601
BsaBI GATnn'nnATC
Cuts at: 2205 2237 5407 2205
Size: 32 3170 2211
Fragments arranged by size:
3170 2211 32
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3220 3602 4237 275
Size: 53 83 186 936 1687 382 635 1451
Fragments arranged by size:
1687 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2688 3846 4244 4518 4665 5076 2688
Size: 1158 398 274 147 411 3025
Fragments arranged by size:
3025 1158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5188
Fragments arranged by size:
5188 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5413
BseYI C'CCAG_C
Cuts at: 2102 2134 4616 5112 2102
Size: 32 2482 496 2403
Fragments arranged by size:
2482 2403 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4840
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3474 3559 4630 3474
Size: 85 1071 4257
Fragments arranged by size:
4257 1071 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2732 3474 3559 4630 1391
Size: 399 319 623 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 623 399 319 85
BspEI T'CCGG_A
Cuts at: 2688 5076 2688
Size: 2388 3025
BspHI T'CATG_A
Cuts at: 2203 3302 5032 2203
Size: 1099 1730 2584
Fragments arranged by size:
2584 1730 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 844
Size: 1924 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4057 4244 1700
Size: 110 2247 187 2869
Fragments arranged by size:
2869 2247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5413
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 2485 1184
Size: 1301 4112
BstYI r'GATC_y
Cuts at: 1972 2238 2743 3110 3495 3512 4964 5050 5062
Size: 266 505 367 385 17 1452 86 12
Cuts at: 5062 5408 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 505 385 367 346 266 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2631 513
Size: 552 939 627 3295
Fragments arranged by size:
3295 939 627 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3723 3743 2929
Size: 794 20 4599
Fragments arranged by size:
4599 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 2860 3749
Size: 54 1425 50 275 18 798 232 889
Cuts at: 3749 5378 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 798 275 232 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 2663 3343 1353
Size: 797 513 680 3423
Fragments arranged by size:
3423 797 680 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3476 4073 4860 5128 1239
Size: 393 1844 597 787 268 1524
Fragments arranged by size:
1844 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2042 2042
Size: 5413
EcoRI G'AATT_C
Cuts at: 2519 2722 3100 2519
Size: 203 378 4832
Fragments arranged by size:
4832 378 203
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 4560 1678
Size: 2882 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 4054 4086 4128
Size: 32 135 32 1055 32 1395 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1395 1055 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3003 669
Size: 988 281 819 246 3079
Fragments arranged by size:
3079 988 819 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 4527 4711 1305
Size: 1138 2084 184 2007
Fragments arranged by size:
2084 2007 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 10
Size: 54 1425 343 798 2793
Fragments arranged by size:
2793 1425 798 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 3371 3730 3889 518
Size: 648 1002 1203 359 159 2042
Fragments arranged by size:
2042 1203 1002 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2285 2526 2829 3506 4654 4899 1606
Size: 496 183 241 303 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 496 303 245 241 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 2631 513
Size: 552 939 627 3295
Fragments arranged by size:
3295 939 627 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2229 2983 2229
Size: 754 4659
PstI C_TGCA'G
Cuts at: 830 2765 830
Size: 1935 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
PvuII CAG'CTG
Cuts at: 2285 2526 2285
Size: 241 5172
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 2625 3661 5373 2625
Size: 1036 1712 2665
Fragments arranged by size:
2665 1712 1036
SfcI C'TryA_G
Cuts at: 826 1045 2089 2761 3896 4577 4768 826
Size: 219 1044 672 1135 681 191 1471
Fragments arranged by size:
1471 1135 1044 681 672 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3517 4418 4680 820
Size: 197 926 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2603 3204 493
Size: 2110 601 2702
Fragments arranged by size:
2702 2110 601
SpeI A'CTAG_T
Cuts at: 152 2125 152
Size: 1973 3440
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2004 2490 2508 2631 2852 513
Size: 552 939 486 18 123 221 3074
Fragments arranged by size:
3074 939 552 486 221 123 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 3440 3457 3610 3795 4240 325
Size: 1255 546 1314 17 153 185 445 1498
Fragments arranged by size:
1498 1314 1255 546 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2623 3659
Size: 274 80 33 51 1443 141 505 1036
Cuts at: 3659 5371 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1036 505 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5373 5405 5373
Size: 32 5381
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI