Restriction map for
pFN21AE4026
(Circular) (Six-base) MAPSORT of: pFN21AE4026.seq Check: 3743 from: 1 to: 5407
pFN21AE4026 5407 bp
With 182 enzymes: SgfI *
March 28, 2012 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2446 2446
Size: 5407
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4854 5122 1632
Size: 1838 1384 268 1917
Fragments arranged by size:
1917 1838 1384 268
AfeI AGC'GCT
Cuts at: 2092 2092
Size: 5407
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 2150 4136 2150
Size: 1986 3421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 2188 3150 4722 2188
Size: 962 1572 2873
Fragments arranged by size:
2873 1572 962
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2384 2716 2942 3094 5203 1493
Size: 891 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 891 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2405 3961 160
Size: 872 1373 1556 1606
Fragments arranged by size:
1606 1556 1373 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2732 1870
Size: 73 97 692 4545
Fragments arranged by size:
4545 692 97 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2159 2267 2458 2728
Size: 325 217 627 315 57 108 191 270
Cuts at: 2728 4183 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 315 270 217 191
108 57
BanII G_rGCy'C
Cuts at: 1391 2028 2100 2508 2664 2726 1391
Size: 637 72 408 156 62 4072
Fragments arranged by size:
4072 637 408 156 72 62
BbeI G_GCGC'C
Cuts at: 2106 2271 2106
Size: 165 5242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 928
Size: 971 699 3737
Fragments arranged by size:
3737 971 699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4067 1239
Size: 2828 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2683 3532 4397 4695 2683
Size: 849 865 298 3395
Fragments arranged by size:
3395 865 849 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2280 4070 882
Size: 617 781 1790 2219
Fragments arranged by size:
2219 1790 781 617
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2268 3214 3596
Size: 53 83 186 936 570 165 946 382
Cuts at: 3596 4231 275
Size: 635 1451
Fragments arranged by size:
1451 946 936 635 570 382 186 165
83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5182
Fragments arranged by size:
5182 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2516 2560 1186
Size: 1327 3 44 4033
Fragments arranged by size:
4033 1327 44 3
BseYI C'CCAG_C
Cuts at: 2062 2093 2112 2663 4610 5106 2062
Size: 31 19 551 1947 496 2363
Fragments arranged by size:
2363 1947 551 496 31 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2615 1163
Size: 573 879 3955
Fragments arranged by size:
3955 879 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2508 3468 3553 4624 2028
Size: 480 960 85 1071 2811
Fragments arranged by size:
2811 1071 960 480 85
BsiWI C'GTAC_G
Cuts at: 2643 2643
Size: 5407
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2100 2508 2664 2726 3468 3553
Size: 399 238 72 408 156 62 742 85
Cuts at: 3553 4624 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 238 156 85
72 62
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2446 2762 844
Size: 1602 316 3489
Fragments arranged by size:
3489 1602 316
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 1394
Size: 429 1471 3507
Fragments arranged by size:
3507 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 4051 4238 1700
Size: 110 225 2016 187 2869
Fragments arranged by size:
2869 2016 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 2120 2120
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402
Size: 765 367 385 17 1452 86 12 346
Cuts at: 5402 1972
Size: 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3916
Fragments arranged by size:
3916 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 487
Size: 683 56 1210 3458
Fragments arranged by size:
3458 1210 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 2495 2923 3717 3737 2017
Size: 478 428 794 20 3687
Fragments arranged by size:
3687 794 478 428 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2281 2316 529
Size: 35 1717 35 3620
Fragments arranged by size:
3620 1717 35 35
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2652 2854 3743
Size: 54 1425 50 275 18 822 202 889
Cuts at: 3743 5372 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 822 275 202 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3994 4368 4514 387
Size: 1064 138 435 1970 374 146 1280
Fragments arranged by size:
1970 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 2506 2026
Size: 480 4927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3470 4067 4854 5122 1239
Size: 393 1838 597 787 268 1524
Fragments arranged by size:
1838 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2357
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 2197 2197
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2094 2106 2119 2271 4554 1678
Size: 416 12 13 152 2283 2531
Fragments arranged by size:
2531 2283 416 152 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2136 2168 2189
Size: 32 135 32 443 32 89 32 21
Cuts at: 2189 2221 2231 2263 4048 4080 4122 4154 1373
Size: 32 10 32 1785 32 42 32 2626
Fragments arranged by size:
2626 1785 443 135 89 42 32 32
32 32 32 32 32 32 21 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2102 2267 2102
Size: 165 5242
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2167 2352 2556 4521 4705 1305
Size: 862 185 204 1965 184 2007
Fragments arranged by size:
2007 1965 862 204 185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3585
Fragments arranged by size:
3585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3365 3724 3883 518
Size: 648 1347 852 359 159 2042
Fragments arranged by size:
2042 1347 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2823 3500 4648 4893 1606
Size: 479 738 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 479 245
NaeI GCC'GGC
Cuts at: 2037 2037
Size: 5407
NarI GG'CG_CC
Cuts at: 2103 2268 2103
Size: 165 5242
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3916
Fragments arranged by size:
3916 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2035 2035
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2474 2765 4310 1132
Size: 97 1245 291 1545 2229
Fragments arranged by size:
2229 1545 1245 291 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2197
Size: 5407
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2090 2490 2759 830
Size: 1260 400 269 3478
Fragments arranged by size:
3478 1260 400 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SacI G_AGCT'C
Cuts at: 2028 2508 2028
Size: 480 4927
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SanDI GG'GwC_CC
Cuts at: 2197 2197
Size: 5407
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2086 2486 2755 3890 4571 4762 826
Size: 219 1041 400 269 1135 681 191 1471
Fragments arranged by size:
1471 1135 1041 681 400 269 219 191
SfoI GGC'GCC
Cuts at: 2104 2269 2104
Size: 165 5242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2042 2734 1872
Size: 170 692 4545
Fragments arranged by size:
4545 692 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2698 3511 4412 4674 820
Size: 197 926 755 813 901 262 1553
Fragments arranged by size:
1553 926 901 813 755 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2004 2622 2846 513
Size: 552 939 618 224 3074
Fragments arranged by size:
3074 939 618 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3653 5365 96
Size: 274 80 33 51 1443 1676 1712 138
Fragments arranged by size:
1712 1676 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2040 2732 1870
Size: 170 692 4545
Fragments arranged by size:
4545 692 170
XmnI GAAnn'nnTTC
Cuts at: 2207 3536 2207
Size: 1329 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI