Restriction map for
pFN21AE4023
(Circular) (Six-base) MAPSORT of: pFN21AE4023.seq Check: 5336 from: 1 to: 5338
pFN21AE4023 5338 bp
With 182 enzymes: SgfI *
March 28, 2012 13:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2304 2556 3148 278
Size: 53 83 186 936 768 252 592 2468
Fragments arranged by size:
2468 936 768 592 252 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2612 3401 4785 5053 1632
Size: 452 528 789 1384 268 1917
Fragments arranged by size:
1917 1384 789 528 452 268
AfeI AGC'GCT
Cuts at: 2587 2587
Size: 5338
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 2479 3081 4653 2479
Size: 602 1572 3164
Fragments arranged by size:
3164 1572 602
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 5338
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2138 2663 1870
Size: 73 72 123 525 4545
Fragments arranged by size:
4545 525 123 73 72
BamHI G'GATC_C
Cuts at: 2373 2668 3035 2373
Size: 295 367 4676
Fragments arranged by size:
4676 367 295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2659 4114 618
Size: 325 217 627 872 1455 1842
Fragments arranged by size:
1842 1455 872 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2132 2460 2657 1391
Size: 632 109 328 197 4072
Fragments arranged by size:
4072 632 328 197 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2548 928
Size: 971 345 304 3718
Fragments arranged by size:
3718 971 345 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 2576 3949
Size: 107 122 71 631 605 346 558 1373
Cuts at: 3949 136
Size: 1525
Fragments arranged by size:
1525 1373 631 605 558 346 122 107
71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2023 3399 4555 1790
Size: 233 1376 1156 2573
Fragments arranged by size:
2573 1376 1156 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2525 2525
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 3998 1239
Size: 1235 1524 2579
Fragments arranged by size:
2579 1524 1235
Bpu10I CC'TnA_GC
Cuts at: 2480 2480
Size: 5338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 2072 5332 2072
Size: 3260 2078
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 2553 3145 3527
Size: 53 83 186 936 768 252 592 382
Cuts at: 3527 4162 275
Size: 635 1451
Fragments arranged by size:
1451 936 768 635 592 382 252 186
83 53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 2448 4541 5037 2448
Size: 2093 496 2749
Fragments arranged by size:
2749 2093 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2229 2788 3549 4577 1540
Size: 275 186 228 559 761 1028 2301
Fragments arranged by size:
2301 1028 761 559 275 228 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2289 2388 3399 3484 4555 2145
Size: 144 99 1011 85 1071 2928
Fragments arranged by size:
2928 1071 1011 144 99 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2132 2145 2289 2388 2460 2657
Size: 399 233 109 13 144 99 72 197
Cuts at: 2657 3399 3484 4555 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 233 197 144 109
99 85 72 13
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 2304 3227 4957 2304
Size: 923 1730 2685
Fragments arranged by size:
2685 1730 923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2247 2619 3982 4169 1700
Size: 110 437 372 1363 187 2869
Fragments arranged by size:
2869 1363 437 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 2118 96
Size: 2022 3316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 2282 3398 4410 2282
Size: 1116 1012 3210
Fragments arranged by size:
3210 1116 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 2203 1184
Size: 1019 4319
BstYI r'GATC_y
Cuts at: 1972 2373 2496 2668 3035 3420 3437 4889 4975
Size: 401 123 172 367 385 17 1452 86
Cuts at: 4975 4987 5333 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 401 385 367 346 172 123
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2349 2408 2349
Size: 59 5279
BtgI C'CryG_G
Cuts at: 513 1065 2004 2368 2379 513
Size: 552 939 364 11 3472
Fragments arranged by size:
3472 939 552 364 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DraIII CAC_nnn'GTG
Cuts at: 2284 2284
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2226 2785
Size: 54 1425 50 275 18 345 51 559
Cuts at: 2785 3674 5303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 559 345 275 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2226 2785 1537
Size: 275 414 559 4090
Fragments arranged by size:
4090 559 414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 1353
Size: 1915 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2515 3925 4299 4445 387
Size: 1064 138 926 1410 374 146 1280
Fragments arranged by size:
1410 1280 1064 926 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2474 2612 3401 3998 4785 5053
Size: 393 452 390 138 789 597 787 268
Cuts at: 5053 1239
Size: 1524
Fragments arranged by size:
1524 789 787 597 452 393 390 268
138
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 5338
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2476 2019
Size: 1 456 4881
Fragments arranged by size:
4881 456 1
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2487 2589 2620 4485 1678
Size: 809 102 31 1865 2531
Fragments arranged by size:
2531 1865 809 102 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373
Size: 32 135 32 2407 32 42 32 2626
Fragments arranged by size:
2626 2407 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 3296 3655 3814 518
Size: 648 1302 828 359 159 2042
Fragments arranged by size:
2042 1302 828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2370 2428 2452 2754 3431 4579 4824 1606
Size: 764 58 24 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 764 677 302 245 58 24
NaeI GCC'GGC
Cuts at: 2621 2621
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 2004 2379 513
Size: 552 939 375 3472
Fragments arranged by size:
3472 939 552 375
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NgoMIV G'CCGG_C
Cuts at: 2619 2619
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2473 2696 4241 1132
Size: 97 1244 223 1545 2229
Fragments arranged by size:
2229 1545 1244 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2690 830
Size: 1860 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2428 2452 2428
Size: 24 5314
RsrII CG'GwC_CG
Cuts at: 2045 2045
Size: 5338
SacII CC_GC'GG
Cuts at: 2371 2371
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2686 3821 4502 4693 826
Size: 219 1641 1135 681 191 1471
Fragments arranged by size:
1641 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2017 2665 1872
Size: 145 648 4545
Fragments arranged by size:
4545 648 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2379 2571 2777 513
Size: 552 939 375 192 206 3074
Fragments arranged by size:
3074 939 552 375 206 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2291 3365 3382 3535 3720 4165 325
Size: 1255 711 1074 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 711 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2169 3584
Size: 274 80 33 51 1443 141 51 1415
Cuts at: 3584 5296 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1415 274 141 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 2327 1316
Size: 1011 4327
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2015 2663 1870
Size: 145 648 4545
Fragments arranged by size:
4545 648 145
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2302 2554 3146 276
Size: 53 83 186 936 768 252 592 2468
Fragments arranged by size:
2468 936 768 592 252 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI