(Circular) (Six-base) MAPSORT of: pFN21AE4023.seq Check: 5336 from: 1 to: 5338 pFN21AE4023 5338 bp With 182 enzymes: SgfI * March 28, 2012 13:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2304 2556 3148 278 Size: 53 83 186 936 768 252 592 2468 Fragments arranged by size: 2468 936 768 592 252 186 83 53 AccI GT'mk_AC Cuts at: 1937 2681 1937 Size: 744 4594 Acc65I G'GTAC_C Cuts at: 2659 2659 Size: 5338 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2612 3401 4785 5053 1632 Size: 452 528 789 1384 268 1917 Fragments arranged by size: 1917 1384 789 528 452 268 AfeI AGC'GCT Cuts at: 2587 2587 Size: 5338 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 4169 4169 Size: 5338 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 2479 3081 4653 2479 Size: 602 1572 3164 Fragments arranged by size: 3164 1572 602 ApaI G_GGCC'C Cuts at: 2023 2023 Size: 5338 ApaLI G'TGCA_C Cuts at: 3395 4551 3395 Size: 1156 4182 ApoI r'AATT_y Cuts at: 1493 2647 2873 3025 5134 1493 Size: 1154 226 152 2109 1697 Fragments arranged by size: 2109 1697 1154 226 152 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2138 2663 1870 Size: 73 72 123 525 4545 Fragments arranged by size: 4545 525 123 73 72 BamHI G'GATC_C Cuts at: 2373 2668 3035 2373 Size: 295 367 4676 Fragments arranged by size: 4676 367 295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2659 4114 618 Size: 325 217 627 872 1455 1842 Fragments arranged by size: 1842 1455 872 627 325 217 BanII G_rGCy'C Cuts at: 1391 2023 2132 2460 2657 1391 Size: 632 109 328 197 4072 Fragments arranged by size: 4072 632 328 197 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 2548 928 Size: 971 345 304 3718 Fragments arranged by size: 3718 971 345 304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 2576 3949 Size: 107 122 71 631 605 346 558 1373 Cuts at: 3949 136 Size: 1525 Fragments arranged by size: 1525 1373 631 605 558 346 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 2023 3399 4555 1790 Size: 233 1376 1156 2573 Fragments arranged by size: 2573 1376 1156 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3069 4027 442 Size: 416 53 83 399 24 1652 958 1753 Fragments arranged by size: 1753 1652 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2525 2525 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 3998 1239 Size: 1235 1524 2579 Fragments arranged by size: 2579 1524 1235 Bpu10I CC'TnA_GC Cuts at: 2480 2480 Size: 5338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3129 493 Size: 711 1925 2702 Fragments arranged by size: 2702 1925 711 BsaBI GATnn'nnATC Cuts at: 2072 5332 2072 Size: 3260 2078 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2301 2553 3145 3527 Size: 53 83 186 936 768 252 592 382 Cuts at: 3527 4162 275 Size: 635 1451 Fragments arranged by size: 1451 936 768 635 592 382 252 186 83 53 BsaWI w'CCGG_w Cuts at: 3771 4169 4443 4590 5001 3771 Size: 398 274 147 411 4108 Fragments arranged by size: 4108 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5113 Fragments arranged by size: 5113 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5338 BseYI C'CCAG_C Cuts at: 2448 4541 5037 2448 Size: 2093 496 2749 Fragments arranged by size: 2749 2093 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4765 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2229 2788 3549 4577 1540 Size: 275 186 228 559 761 1028 2301 Fragments arranged by size: 2301 1028 761 559 275 228 186 BsiHKAI G_wGCw'C Cuts at: 2145 2289 2388 3399 3484 4555 2145 Size: 144 99 1011 85 1071 2928 Fragments arranged by size: 2928 1071 1011 144 99 85 BsmI GAATG_Cn' Cuts at: 2849 2942 2849 Size: 93 5245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2132 2145 2289 2388 2460 2657 Size: 399 233 109 13 144 99 72 197 Cuts at: 2657 3399 3484 4555 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 233 197 144 109 99 85 72 13 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 2304 3227 4957 2304 Size: 923 1730 2685 Fragments arranged by size: 2685 1730 923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2693 844 Size: 1849 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3225 1394 Size: 429 1402 3507 Fragments arranged by size: 3507 1402 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2247 2619 3982 4169 1700 Size: 110 437 372 1363 187 2869 Fragments arranged by size: 2869 1363 437 372 187 110 BsrGI T'GTAC_A Cuts at: 96 2118 96 Size: 2022 3316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5338 BssSI C'ACGA_G Cuts at: 2282 3398 4410 2282 Size: 1116 1012 3210 Fragments arranged by size: 3210 1116 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5338 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 2203 1184 Size: 1019 4319 BstYI r'GATC_y Cuts at: 1972 2373 2496 2668 3035 3420 3437 4889 4975 Size: 401 123 172 367 385 17 1452 86 Cuts at: 4975 4987 5333 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 401 385 367 346 172 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2349 2408 2349 Size: 59 5279 BtgI C'CryG_G Cuts at: 513 1065 2004 2368 2379 513 Size: 552 939 364 11 3472 Fragments arranged by size: 3472 939 552 364 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4599 Fragments arranged by size: 4599 683 56 BtsI GCAGTG_nn' Cuts at: 2854 3648 3668 2854 Size: 794 20 4524 Fragments arranged by size: 4524 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DraIII CAC_nnn'GTG Cuts at: 2284 2284 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2226 2785 Size: 54 1425 50 275 18 345 51 559 Cuts at: 2785 3674 5303 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 559 345 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2226 2785 1537 Size: 275 414 559 4090 Fragments arranged by size: 4090 559 414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3268 1353 Size: 1915 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2515 3925 4299 4445 387 Size: 1064 138 926 1410 374 146 1280 Fragments arranged by size: 1410 1280 1064 926 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2474 2612 3401 3998 4785 5053 Size: 393 452 390 138 789 597 787 268 Cuts at: 5053 1239 Size: 1524 Fragments arranged by size: 1524 789 787 597 452 393 390 268 138 EcoNI CCTnn'n_nnAGG Cuts at: 2411 2411 Size: 5338 EcoO109I rG'GnC_Cy Cuts at: 2019 2020 2476 2019 Size: 1 456 4881 Fragments arranged by size: 4881 456 1 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 2487 2589 2620 4485 1678 Size: 809 102 31 1865 2531 Fragments arranged by size: 2531 1865 809 102 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373 Size: 32 135 32 2407 32 42 32 2626 Fragments arranged by size: 2626 2407 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2928 669 Size: 988 281 744 246 3079 Fragments arranged by size: 3079 988 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KpnI G_GTAC'C Cuts at: 2663 2663 Size: 5338 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4452 4636 1305 Size: 3147 184 2007 Fragments arranged by size: 3147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3516 Fragments arranged by size: 3516 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3296 3655 3814 518 Size: 648 1302 828 359 159 2042 Fragments arranged by size: 2042 1302 828 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2370 2428 2452 2754 3431 4579 4824 1606 Size: 764 58 24 302 677 1148 245 2120 Fragments arranged by size: 2120 1148 764 677 302 245 58 24 NaeI GCC'GGC Cuts at: 2621 2621 Size: 5338 NcoI C'CATG_G Cuts at: 513 1065 2004 2379 513 Size: 552 939 375 3472 Fragments arranged by size: 3472 939 552 375 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NgoMIV G'CCGG_C Cuts at: 2619 2619 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2785 2785 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2473 2696 4241 1132 Size: 97 1244 223 1545 2229 Fragments arranged by size: 2229 1545 1244 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5338 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 5338 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5338 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PspOMI G'GGCC_C Cuts at: 2019 2019 Size: 5338 PstI C_TGCA'G Cuts at: 830 2690 830 Size: 1860 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 PvuII CAG'CTG Cuts at: 2428 2452 2428 Size: 24 5314 RsrII CG'GwC_CG Cuts at: 2045 2045 Size: 5338 SacII CC_GC'GG Cuts at: 2371 2371 Size: 5338 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SfcI C'TryA_G Cuts at: 826 1045 2686 3821 4502 4693 826 Size: 219 1641 1135 681 191 1471 Fragments arranged by size: 1641 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2017 2665 1872 Size: 145 648 4545 Fragments arranged by size: 4545 648 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 3442 4343 4605 820 Size: 197 926 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2004 2379 2571 2777 513 Size: 552 939 375 192 206 3074 Fragments arranged by size: 3074 939 552 375 206 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2291 3365 3382 3535 3720 4165 325 Size: 1255 711 1074 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1074 711 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2169 3584 Size: 274 80 33 51 1443 141 51 1415 Cuts at: 3584 5296 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1415 274 141 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 2327 1316 Size: 1011 4327 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2015 2663 1870 Size: 145 648 4545 Fragments arranged by size: 4545 648 145 XmnI GAAnn'nnTTC Cuts at: 3467 3467 Size: 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2302 2554 3146 276 Size: 53 83 186 936 768 252 592 2468 Fragments arranged by size: 2468 936 768 592 252 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI