Restriction map for
pFN21AE4019
(Circular) (Six-base) MAPSORT of: pFN21AE4019.seq Check: 4679 from: 1 to: 5308
pFN21AE4019 5308 bp
With 182 enzymes: SgfI *
March 28, 2012 13:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 1937
Size: 714 4594
Acc65I G'GTAC_C
Cuts at: 2629 2629
Size: 5308
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2597 3371 4755 5023 1632
Size: 480 485 774 1384 268 1917
Fragments arranged by size:
1917 1384 774 485 480 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 4207 5184 1203
Size: 3004 977 1327
Fragments arranged by size:
3004 1327 977
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5308
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaLI G'TGCA_C
Cuts at: 3365 4521 3365
Size: 1156 4152
ApoI r'AATT_y
Cuts at: 1493 2208 2276 2617 2843 2995 5104 1493
Size: 715 68 341 226 152 2109 1697
Fragments arranged by size:
2109 1697 715 341 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2119 2633 1870
Size: 73 116 60 514 4545
Fragments arranged by size:
4545 514 116 73 60
BamHI G'GATC_C
Cuts at: 2638 3005 2638
Size: 367 4941
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2629 4084 618
Size: 325 217 627 336 506 1455 1842
Fragments arranged by size:
1842 1455 627 506 336 325 217
BanII G_rGCy'C
Cuts at: 1391 2627 1391
Size: 1236 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2069 2103 3499 3533 1903
Size: 34 132 34 1396 34 3678
Fragments arranged by size:
3678 1396 132 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 2403 1548
Size: 855 4453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3919 136
Size: 107 122 71 631 605 2247 1525
Fragments arranged by size:
2247 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2565 5303 2565
Size: 2738 2570
BlpI GC'TnA_GC
Cuts at: 2725 2725
Size: 5308
Bme1580I G_kGCm'C
Cuts at: 1790 2126 3369 4525 1790
Size: 336 1243 1156 2573
Fragments arranged by size:
2573 1243 1156 336
BmgBI CAC'GTC
Cuts at: 1318 2179 1318
Size: 861 4447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 3997 442
Size: 416 53 83 399 24 1622 958 1753
Fragments arranged by size:
1753 1622 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2417 1056
Size: 1361 3947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3968 1239
Size: 2729 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3433 4298 4596 3433
Size: 865 298 4145
Fragments arranged by size:
4145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 3971 882
Size: 617 563 1909 2219
Fragments arranged by size:
2219 1909 617 563
BsaAI yAC'GTr
Cuts at: 493 1204 3099 493
Size: 711 1895 2702
Fragments arranged by size:
2702 1895 711
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 3115 3497 4132
Size: 53 83 186 936 549 1033 382 635
Cuts at: 4132 275
Size: 1451
Fragments arranged by size:
1451 1033 936 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2292 2595 3741 4139 4413 4560 4971 2292
Size: 303 1146 398 274 147 411 2629
Fragments arranged by size:
2629 1146 411 398 303 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2293 2323 1178
Size: 30 165 30 890 30 4163
Fragments arranged by size:
4163 890 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5308
BseYI C'CCAG_C
Cuts at: 4511 5007 4511
Size: 496 4812
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4735
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3519 4547 1540
Size: 275 186 757 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3369 3454 4525 2034
Size: 1335 85 1071 2817
Fragments arranged by size:
2817 1335 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2126 2627 3369 3454 4525 1391
Size: 399 244 92 501 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 501 399 244 92 85
BspEI T'CCGG_A
Cuts at: 2595 4971 2595
Size: 2376 2932
BspHI T'CATG_A
Cuts at: 3197 4927 3197
Size: 1730 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2042 2110 3195 1394
Size: 429 219 68 1085 3507
Fragments arranged by size:
3507 1085 429 219 68
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3952 4139 1700
Size: 110 2142 187 2869
Fragments arranged by size:
2869 2142 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5308
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5308
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 2113 1184
Size: 929 4379
BstXI CCAn_nnnn'nTGG
Cuts at: 2557 2557
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2565 2638 3005 3390 3407 4859 4945 4957
Size: 593 73 367 385 17 1452 86 12
Cuts at: 4957 5303 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 593 385 367 346 86 73
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 3769
Fragments arranged by size:
3769 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2438 487
Size: 683 56 1212 3357
Fragments arranged by size:
3357 1212 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 2824 3618 3638 2424
Size: 400 794 20 4094
Fragments arranged by size:
4094 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2049 2755
Size: 54 1425 50 275 18 207 12 706
Cuts at: 2755 3644 5273 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 706 275 207 54 50
43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2755 1537
Size: 275 943 4090
Fragments arranged by size:
4090 943 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2573 3238 1353
Size: 735 485 665 3423
Fragments arranged by size:
3423 735 665 485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 3895 4269 4415 387
Size: 1064 138 579 1727 374 146 1280
Fragments arranged by size:
1727 1280 1064 579 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2597 3371 3968 4755 5023 1239
Size: 393 480 485 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 597 485 480 393 268
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FspI TGC'GCA
Cuts at: 3814 3814
Size: 5308
HaeII r_GCGC'y
Cuts at: 1678 4455 1678
Size: 2777 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 3949 3981 4023
Size: 32 135 32 641 32 1704 32 42
Cuts at: 4023 4055 1373
Size: 32 2626
Fragments arranged by size:
2626 1704 641 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 2898 669
Size: 988 281 714 246 3079
Fragments arranged by size:
3079 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2633 2633
Size: 5308
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 10
Size: 54 1425 343 219 3267
Fragments arranged by size:
3267 1425 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2177 2262 3266 3625 3784 518
Size: 648 963 48 85 1004 359 159 2042
Fragments arranged by size:
2042 1004 963 648 359 159 85 48
MspA1I CmG'CkG
Cuts at: 1606 2432 2724 3401 4549 4794 1606
Size: 826 292 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 677 292 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3817
Fragments arranged by size:
3817 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 2413 1052
Size: 1361 3947
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5308
PfoI T'CCnGG_A
Cuts at: 2166 2463 2166
Size: 297 5011
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 4890 4922 3377
Size: 32 1481 32 3763
Fragments arranged by size:
3763 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PstI C_TGCA'G
Cuts at: 830 2660 830
Size: 1830 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
PvuII CAG'CTG
Cuts at: 2432 2432
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2656 3791 4472 4663 826
Size: 219 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2635 1872
Size: 763 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4313 4575 820
Size: 197 926 1469 901 262 1553
Fragments arranged by size:
1553 1469 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2747 513
Size: 552 939 743 3074
Fragments arranged by size:
3074 939 743 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3335 3352 3505 3690 4135 325
Size: 1255 1755 17 153 185 445 1498
Fragments arranged by size:
1755 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3554 5266 96
Size: 274 80 33 51 1443 1577 1712 138
Fragments arranged by size:
1712 1577 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2293 2325 5268 5300 2293
Size: 32 2943 32 2301
Fragments arranged by size:
2943 2301 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2633 1870
Size: 763 4545
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI