Restriction map for
pFN21AE4013
(Circular) (Six-base) MAPSORT of: pFN21AE4013.seq Check: 4966 from: 1 to: 5269
pFN21AE4013 5269 bp
With 182 enzymes: SgfI *
March 28, 2012 13:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3079 278
Size: 53 83 186 936 1543 2468
Fragments arranged by size:
2468 1543 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2423 2612 1937
Size: 486 189 4594
Fragments arranged by size:
4594 486 189
Acc65I G'GTAC_C
Cuts at: 2590 2590
Size: 5269
AclI AA'CG_TT
Cuts at: 1563 3396 3769 1563
Size: 1833 373 3063
Fragments arranged by size:
3063 1833 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3332 4716 4984 1632
Size: 1700 1384 268 1917
Fragments arranged by size:
1917 1700 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5072
AflIII A'CryG_T
Cuts at: 1203 4168 5145 1203
Size: 2965 977 1327
Fragments arranged by size:
2965 1327 977
AgeI A'CCGG_T
Cuts at: 4100 4100
Size: 5269
AhdI GACnn_n'nnGTC
Cuts at: 3998 3998
Size: 5269
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2401 1184
Size: 1217 4052
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2400 2432 2400
Size: 32 5237
AlwNI CAG_nnn'CTG
Cuts at: 2083 3012 4584 2083
Size: 929 1572 2768
Fragments arranged by size:
2768 1572 929
ApaI G_GGCC'C
Cuts at: 2458 2458
Size: 5269
ApaLI G'TGCA_C
Cuts at: 3326 4482 3326
Size: 1156 4113
ApoI r'AATT_y
Cuts at: 1493 2194 2273 2578 2804 2956 5065 1493
Size: 701 79 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 701 305 226 152 79
AseI AT'TA_AT
Cuts at: 160 1032 3823 160
Size: 872 2791 1606
Fragments arranged by size:
2791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
AvaI C'yCGr_G
Cuts at: 1870 1943 2594 1870
Size: 73 651 4545
Fragments arranged by size:
4545 651 73
BamHI G'GATC_C
Cuts at: 2599 2966 2599
Size: 367 4902
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 4045 618
Size: 325 217 627 803 1455 1842
Fragments arranged by size:
1842 1455 803 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2458 2588 1391
Size: 1067 130 4072
Fragments arranged by size:
4072 1067 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2187 928
Size: 971 288 4010
Fragments arranged by size:
4010 971 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3460 3494 1903
Size: 34 1523 34 3678
Fragments arranged by size:
3678 1523 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 4371 3161
Size: 1210 4059
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3880 136
Size: 107 122 71 631 605 2208 1525
Fragments arranged by size:
2208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2121 5264 2121
Size: 3143 2126
BlpI GC'TnA_GC
Cuts at: 2686 2686
Size: 5269
Bme1580I G_kGCm'C
Cuts at: 1790 2458 3330 4486 1790
Size: 668 872 1156 2573
Fragments arranged by size:
2573 1156 872 668
BmgBI CAC'GTC
Cuts at: 1318 2449 1318
Size: 1131 4138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3000 3958 442
Size: 416 53 83 399 24 1583 958 1753
Fragments arranged by size:
1753 1583 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 2489 2495 2497 3929 1239
Size: 939 311 6 2 1432 2579
Fragments arranged by size:
2579 1432 939 311 6 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3394 4259 4557 3394
Size: 865 298 4106
Fragments arranged by size:
4106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2100 3932 882
Size: 617 601 1832 2219
Fragments arranged by size:
2219 1832 617 601
BsaAI yAC'GTr
Cuts at: 493 1204 3060 493
Size: 711 1856 2702
Fragments arranged by size:
2702 1856 711
BsaBI GATnn'nnATC
Cuts at: 5263 5263
Size: 5269
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3076 3458 4093
Size: 53 83 186 936 476 1067 382 635
Cuts at: 4093 275
Size: 1451
Fragments arranged by size:
1451 1067 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3702 4100 4374 4521 4932 3702
Size: 398 274 147 411 4039
Fragments arranged by size:
4039 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2425 2455 1178
Size: 30 165 30 1022 30 3992
Fragments arranged by size:
3992 1022 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5269
BseYI C'CCAG_C
Cuts at: 4472 4968 4472
Size: 496 4773
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4696
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2719 3480 4508 1540
Size: 275 186 718 761 1028 2301
Fragments arranged by size:
2301 1028 761 718 275 186
BsiHKAI G_wGCw'C
Cuts at: 3330 3415 4486 3330
Size: 85 1071 4113
Fragments arranged by size:
4113 1071 85
BsmI GAATG_Cn'
Cuts at: 2780 2873 2780
Size: 93 5176
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2458 2588 3330 3415 4486 1391
Size: 399 668 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 399 130 85
BspEI T'CCGG_A
Cuts at: 4932 4932
Size: 5269
BspHI T'CATG_A
Cuts at: 3158 4888 3158
Size: 1730 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 2624 844
Size: 1591 189 3489
Fragments arranged by size:
3489 1591 189
BsrBI CCG'CTC
Cuts at: 1394 1823 3156 1394
Size: 429 1333 3507
Fragments arranged by size:
3507 1333 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3764 3938 65
Size: 1183 2516 174 1396
Fragments arranged by size:
2516 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3913 4100 1700
Size: 110 2103 187 2869
Fragments arranged by size:
2869 2103 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5269
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5269
BssSI C'ACGA_G
Cuts at: 3329 4341 3329
Size: 1012 4257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5269
BstBI TT'CG_AA
Cuts at: 4073 4073
Size: 5269
BstEII G'GTnAC_C
Cuts at: 1184 2086 1184
Size: 902 4367
BstYI r'GATC_y
Cuts at: 1972 2121 2332 2599 2966 3351 3368 4820 4906
Size: 149 211 267 367 385 17 1452 86
Cuts at: 4906 4918 5264 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 267 211 149
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3778
Fragments arranged by size:
3778 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4530
Fragments arranged by size:
4530 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 3579 3599 2785
Size: 794 20 4455
Fragments arranged by size:
4455 794 20
ClaI AT'CG_AT
Cuts at: 1554 2143 1554
Size: 589 4680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5234
DraI TTT'AAA
Cuts at: 2573 2573
Size: 5269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4276 809
Size: 507 2960 1802
Fragments arranged by size:
2960 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2716 3605 5234
Size: 54 1425 50 275 18 886 889 1629
Cuts at: 5234 8
Size: 43
Fragments arranged by size:
1629 1425 889 886 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2716 1537
Size: 275 904 4090
Fragments arranged by size:
4090 904 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3199 1353
Size: 1846 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2477 3856 4230 4376 387
Size: 1064 138 888 1379 374 146 1280
Fragments arranged by size:
1379 1280 1064 888 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2489 2495 2497 3332 3929 4716
Size: 393 546 311 6 2 835 597 787
Cuts at: 4716 4984 1239
Size: 268 1524
Fragments arranged by size:
1524 835 787 597 546 393 311 268
6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2462
Size: 5269
EcoO109I rG'GnC_Cy
Cuts at: 2454 2455 2454
Size: 1 5268
EcoRI G'AATT_C
Cuts at: 2194 2578 2956 2194
Size: 384 378 4507
Fragments arranged by size:
4507 384 378
FspI TGC'GCA
Cuts at: 3775 3775
Size: 5269
HaeII r_GCGC'y
Cuts at: 1678 4416 1678
Size: 2738 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2129 2157 2161 3910
Size: 32 135 32 553 4 28 4 1749
Cuts at: 3910 3942 3984 4016 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1749 553 135 42 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2424 2613 2859 669
Size: 988 281 486 189 246 3079
Fragments arranged by size:
3079 988 486 281 246 189
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5269
HpaI GTT'AAC
Cuts at: 2859 2859
Size: 5269
KpnI G_GTAC'C
Cuts at: 2594 2594
Size: 5269
MfeI C'AATT_G
Cuts at: 2868 2868
Size: 5269
MluI A'CGCG_T
Cuts at: 5145 5145
Size: 5269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4383 4567 1305
Size: 3078 184 2007
Fragments arranged by size:
3078 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3447
Fragments arranged by size:
3447 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 3586 3745 518
Size: 648 2061 359 159 2042
Fragments arranged by size:
2061 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 2515 2685 3362 4510 4755 1606
Size: 879 30 170 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 245 170 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3778
Fragments arranged by size:
3778 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5269
NotI GC'GGCC_GC
Cuts at: 2716 2716
Size: 5269
NspI r_CATG'y
Cuts at: 1132 1229 2392 2627 4172 1132
Size: 97 1163 235 1545 2229
Fragments arranged by size:
2229 1545 1163 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5269
PciI A'CATG_T
Cuts at: 4168 4168
Size: 5269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 1108
Size: 975 4294
PmeI GTTT'AAAC
Cuts at: 2573 2573
Size: 5269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 4851 4883 3338
Size: 32 1481 32 3724
Fragments arranged by size:
3724 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5269
PsiI TTA'TAA
Cuts at: 2839 2839
Size: 5269
PspOMI G'GGCC_C
Cuts at: 2454 2454
Size: 5269
PstI C_TGCA'G
Cuts at: 830 2621 830
Size: 1791 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5269
PvuII CAG'CTG
Cuts at: 2485 2485
Size: 5269
SalI G'TCGA_C
Cuts at: 1936 2422 2611 1936
Size: 486 189 4594
Fragments arranged by size:
4594 486 189
SbfI CC_TGCA'GG
Cuts at: 2621 2621
Size: 5269
ScaI AGT'ACT
Cuts at: 3517 5229 3517
Size: 1712 3557
SfcI C'TryA_G
Cuts at: 826 1045 2040 2617 3752 4433 4624 826
Size: 219 995 577 1135 681 191 1471
Fragments arranged by size:
1471 1135 995 681 577 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5269
SmaI CCC'GGG
Cuts at: 1872 2596 1872
Size: 724 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3373 4274 4536 820
Size: 197 926 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3060 493
Size: 2567 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5269
SphI G_CATG'C
Cuts at: 1132 2627 1132
Size: 1495 3774
SspI AAT'ATT
Cuts at: 5 52 2226 2241 3193 5
Size: 47 2174 15 952 2081
Fragments arranged by size:
2174 2081 952 47 15
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2708 513
Size: 552 939 704 3074
Fragments arranged by size:
3074 939 704 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3296 3313 3466 3651 4096 325
Size: 1255 1716 17 153 185 445 1498
Fragments arranged by size:
1716 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3515 5227 96
Size: 274 80 33 51 1443 1538 1712 138
Fragments arranged by size:
1712 1538 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2425 2457 5229 5261 2425
Size: 32 2772 32 2433
Fragments arranged by size:
2772 2433 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2249 1316
Size: 933 4336
XbaI T'CTAG_A
Cuts at: 2605 2605
Size: 5269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5269
XmaI C'CCGG_G
Cuts at: 1870 2594 1870
Size: 724 4545
XmnI GAAnn'nnTTC
Cuts at: 3398 3398
Size: 5269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3077 276
Size: 53 83 186 936 1543 2468
Fragments arranged by size:
2468 1543 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI