Restriction map for
pFN21AE4002
(Circular) (Six-base) MAPSORT of: pFN21AE4002.seq Check: 4329 from: 1 to: 5314
pFN21AE4002 5314 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2243
Size: 5314
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3124 278
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 2657 1937
Size: 340 380 4594
Fragments arranged by size:
4594 380 340
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 3441 3814 1563
Size: 1878 373 3063
Fragments arranged by size:
3063 1878 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2497 3377 4761 5029 1632
Size: 865 880 1384 268 1917
Fragments arranged by size:
1917 1384 880 865 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 2410 2430 4213 5190 1203
Size: 1207 20 1783 977 1327
Fragments arranged by size:
1783 1327 1207 977 20
AgeI A'CCGG_T
Cuts at: 4145 4145
Size: 5314
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2251 2573 1184
Size: 1067 322 3925
Fragments arranged by size:
3925 1067 322
AlwNI CAG_nnn'CTG
Cuts at: 2335 3057 4629 2335
Size: 722 1572 3020
Fragments arranged by size:
3020 1572 722
ApaLI G'TGCA_C
Cuts at: 2159 2558 3371 4527 2159
Size: 399 813 1156 2946
Fragments arranged by size:
2946 1156 813 399
ApoI r'AATT_y
Cuts at: 1493 2623 2849 3001 5110 1493
Size: 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3868 160
Size: 872 2836 1606
Fragments arranged by size:
2836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 1870
Size: 73 696 4545
Fragments arranged by size:
4545 696 73
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 4090 618
Size: 325 217 627 848 1455 1842
Fragments arranged by size:
1842 1455 848 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2633 1391
Size: 1242 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 1903
Size: 34 1568 34 3678
Fragments arranged by size:
3678 1568 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4416 3206
Size: 1210 4104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5309 5309
Size: 5314
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2562 3375 4531 1790
Size: 373 399 813 1156 2573
Fragments arranged by size:
2573 1156 813 399 373
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 4003 442
Size: 416 53 83 399 24 1628 958 1753
Fragments arranged by size:
1753 1628 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2201 2357 3974 1239
Size: 914 48 156 1617 2579
Fragments arranged by size:
2579 1617 914 156 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2510 3439 4304 4602 2510
Size: 929 865 298 3222
Fragments arranged by size:
3222 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 3977 882
Size: 617 515 1963 2219
Fragments arranged by size:
2219 1963 617 515
BsaAI yAC'GTr
Cuts at: 493 1204 2158 2470 2557 3105 493
Size: 711 954 312 87 548 2702
Fragments arranged by size:
2702 954 711 548 312 87
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2366 3121 3503 4138
Size: 53 83 186 936 833 755 382 635
Cuts at: 4138 275
Size: 1451
Fragments arranged by size:
1451 936 833 755 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2250 3747 4145 4419 4566 4977 2250
Size: 1497 398 274 147 411 2587
Fragments arranged by size:
2587 1497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5089
Fragments arranged by size:
5089 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5314
BseYI C'CCAG_C
Cuts at: 2032 4517 5013 2032
Size: 2485 496 2333
Fragments arranged by size:
2485 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4741
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3525 4553 1540
Size: 275 186 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 2562 3375 3460 4531 2163
Size: 399 813 85 1071 2946
Fragments arranged by size:
2946 1071 813 399 85
BsmI GAATG_Cn'
Cuts at: 2480 2825 2918 2480
Size: 345 93 4876
Fragments arranged by size:
4876 345 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2333 2357 2333
Size: 24 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2562 2633 3375 3460 4531 1391
Size: 399 373 399 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 373 85 71
BspEI T'CCGG_A
Cuts at: 2250 4977 2250
Size: 2727 2587
BspHI T'CATG_A
Cuts at: 2145 3203 4933 2145
Size: 1058 1730 2526
Fragments arranged by size:
2526 1730 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2243 2406 2669 844
Size: 1295 104 163 263 3489
Fragments arranged by size:
3489 1295 263 163 104
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 1394
Size: 429 1378 3507
Fragments arranged by size:
3507 1378 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3809 3983 65
Size: 1183 2561 174 1396
Fragments arranged by size:
2561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3958 4145 1700
Size: 110 2148 187 2869
Fragments arranged by size:
2869 2148 187 110
BsrGI T'GTAC_A
Cuts at: 96 2079 96
Size: 1983 3331
BssHII G'CGCG_C
Cuts at: 1925 2583 1925
Size: 658 4656
BssSI C'ACGA_G
Cuts at: 3374 4386 3374
Size: 1012 4302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2503 2601 794
Size: 1709 98 3507
Fragments arranged by size:
3507 1709 98
BstBI TT'CG_AA
Cuts at: 4118 4118
Size: 5314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2172 2331 2644 3011 3396 3413 4865 4951
Size: 200 159 313 367 385 17 1452 86
Cuts at: 4951 4963 5309 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 313 200 159
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3823
Fragments arranged by size:
3823 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4575
Fragments arranged by size:
4575 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3624 3644 2830
Size: 794 20 4500
Fragments arranged by size:
4500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4321 809
Size: 507 3005 1802
Fragments arranged by size:
3005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2152 2481
Size: 54 1425 50 275 18 195 127 329
Cuts at: 2481 2761 3650 5279 8
Size: 280 889 1629 43
Fragments arranged by size:
1629 1425 889 329 280 275 195 127
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2761 1537
Size: 275 949 4090
Fragments arranged by size:
4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2516 3244 1353
Size: 1163 728 3423
Fragments arranged by size:
3423 1163 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2312 3901 4275 4421 387
Size: 1064 138 723 1589 374 146 1280
Fragments arranged by size:
1589 1280 1064 723 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2201 2357 2497 3377 3974 4761
Size: 393 521 48 156 140 880 597 787
Cuts at: 4761 5029 1239
Size: 268 1524
Fragments arranged by size:
1524 880 787 597 521 393 268 156
140 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2239 2239
Size: 5314
EcoO109I rG'GnC_Cy
Cuts at: 2395 2395
Size: 5314
EcoRI G'AATT_C
Cuts at: 2623 3001 2623
Size: 378 4936
FspI TGC'GCA
Cuts at: 3820 3820
Size: 5314
HaeII r_GCGC'y
Cuts at: 1678 2199 4461 1678
Size: 521 2262 2531
Fragments arranged by size:
2531 2262 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 3955 3987 4029
Size: 32 135 32 828 32 1523 32 42
Cuts at: 4029 4061 1373
Size: 32 2626
Fragments arranged by size:
2626 1523 828 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2913 2913
Size: 5314
MluI A'CGCG_T
Cuts at: 2410 5190 2410
Size: 2780 2534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4428 4612 1305
Size: 3123 184 2007
Fragments arranged by size:
3123 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3492
Fragments arranged by size:
3492 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3272 3631 3790 518
Size: 648 1212 894 359 159 2042
Fragments arranged by size:
2042 1212 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2601 2730 3407 4555 4800 1606
Size: 995 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 677 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3823
Fragments arranged by size:
3823 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5314
NotI GC'GGCC_GC
Cuts at: 2761 2761
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2672 4217 1132
Size: 97 1443 1545 2229
Fragments arranged by size:
2229 1545 1443 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5314
PciI A'CATG_T
Cuts at: 4213 4213
Size: 5314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PfoI T'CCnGG_A
Cuts at: 2217 2334 2217
Size: 117 5197
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PmlI CAC'GTG
Cuts at: 2158 2557 2158
Size: 399 4915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2395
Size: 5314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2072 2104 2072
Size: 32 5282
PstI C_TGCA'G
Cuts at: 830 2243 2403 2666 830
Size: 1413 160 263 3478
Fragments arranged by size:
3478 1413 263 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SapI GCTCTTCn'nnn_
Cuts at: 2516 2516
Size: 5314
SbfI CC_TGCA'GG
Cuts at: 2666 2666
Size: 5314
ScaI AGT'ACT
Cuts at: 3562 5274 3562
Size: 1712 3602
SexAI A'CCwGG_T
Cuts at: 2439 2439
Size: 5314
SfcI C'TryA_G
Cuts at: 826 1045 2239 2399 2662 3797 4478 4669 826
Size: 219 1194 160 263 1135 681 191 1471
Fragments arranged by size:
1471 1194 1135 681 263 219 191 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2641 1872
Size: 769 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 3418 4319 4581 820
Size: 197 926 582 893 901 262 1553
Fragments arranged by size:
1553 926 901 893 582 262 197
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5314
SphI G_CATG'C
Cuts at: 1132 2672 1132
Size: 1540 3774
SspI AAT'ATT
Cuts at: 5 52 3238 5
Size: 47 3186 2081
Fragments arranged by size:
3186 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2753 513
Size: 552 939 749 3074
Fragments arranged by size:
3074 939 749 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2203 3341 3358 3511 3696 4141 325
Size: 1255 623 1138 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1138 623 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 2487 3560
Size: 274 80 33 51 1443 102 408 1073
Cuts at: 3560 5272 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1073 408 274 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5274 5306 5274
Size: 32 5282
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5314
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2377 2020
Size: 357 4957
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5314
XmaI C'CCGG_G
Cuts at: 1870 2639 1870
Size: 769 4545
XmnI GAAnn'nnTTC
Cuts at: 3443 3443
Size: 5314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3122 276
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI