Restriction map for
pFN21AE3992
(Circular) (Six-base) MAPSORT of: pFN21AE3992.seq Check: 6847 from: 1 to: 5860
pFN21AE3992 5860 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 2355 3181 2355
Size: 826 5034
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2197 2963 3923 5307 5575 1632
Size: 565 766 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 766 565 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 3002 3603 5175 3002
Size: 601 1572 3687
Fragments arranged by size:
3687 1572 601
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2541 2558 2583 2675 2819 3169 3395 3547
Size: 1048 17 25 92 144 350 226 152
Cuts at: 3547 5656 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1048 350 226 152 144 92
25 17
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3185 1870
Size: 73 1242 4545
Fragments arranged by size:
4545 1242 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2355 2665 3181 4636 618
Size: 325 217 627 568 310 516 1455 1842
Fragments arranged by size:
1842 1455 627 568 516 325 310 217
BanII G_rGCy'C
Cuts at: 1391 3179 1391
Size: 1788 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2203 3036 928
Size: 971 304 833 3752
Fragments arranged by size:
3752 971 833 304
BbvCI CC'TCA_GC
Cuts at: 3051 3051
Size: 5860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 3921 5077 1790
Size: 2131 1156 2573
Fragments arranged by size:
2573 2131 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3591 4549 442
Size: 416 53 83 399 24 2174 958 1753
Fragments arranged by size:
2174 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3007 4520 1239
Size: 1768 1513 2579
Fragments arranged by size:
2579 1768 1513
Bpu10I CC'TnA_GC
Cuts at: 3051 3051
Size: 5860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2223 3985 4850 5148 2223
Size: 1762 865 298 2935
Fragments arranged by size:
2935 1762 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 4523 882
Size: 617 504 2520 2219
Fragments arranged by size:
2520 2219 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2403 1186
Size: 1217 4643
BseYI C'CCAG_C
Cuts at: 2078 2373 5063 5559 2078
Size: 295 2690 496 2379
Fragments arranged by size:
2690 2379 496 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3921 4006 5077 3921
Size: 85 1071 4704
Fragments arranged by size:
4704 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3179 3921 4006 5077 1391
Size: 399 1389 742 85 1071 2174
Fragments arranged by size:
2174 1389 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 2416 3749 5479 2416
Size: 1333 1730 2797
Fragments arranged by size:
2797 1730 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3079 4355 4529 65
Size: 1183 1831 1276 174 1396
Fragments arranged by size:
1831 1396 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2782 4504 4691 1700
Size: 110 972 1722 187 2869
Fragments arranged by size:
2869 1722 972 187 110
BsrGI T'GTAC_A
Cuts at: 96 2526 96
Size: 2430 3430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2890 794
Size: 2096 3764
BstBI TT'CG_AA
Cuts at: 2500 4664 2500
Size: 2164 3696
BstEII G'GTnAC_C
Cuts at: 1184 2861 1184
Size: 1677 4183
BstXI CCAn_nnnn'nTGG
Cuts at: 2020 2020
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855
Size: 1218 367 385 17 1452 86 12 346
Cuts at: 5855 1972
Size: 1977
Fragments arranged by size:
1977 1452 1218 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2969 3063 513
Size: 552 939 965 94 3310
Fragments arranged by size:
3310 965 939 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2888 3376 4170 4190 2888
Size: 488 794 20 4558
Fragments arranged by size:
4558 794 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 2937 3164 2937
Size: 227 5633
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 3307 4196
Size: 54 1425 50 275 18 855 622 889
Cuts at: 4196 5825 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 855 622 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2572 3790 1353
Size: 1219 1218 3423
Fragments arranged by size:
3423 1219 1218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2813 4447 4821 4967 387
Size: 1064 138 1224 1634 374 146 1280
Fragments arranged by size:
1634 1280 1224 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2963 3007 3923 4520 5307 5575
Size: 393 565 766 44 916 597 787 268
Cuts at: 5575 1239
Size: 1524
Fragments arranged by size:
1524 916 787 766 597 565 393 268
44
EcoRI G'AATT_C
Cuts at: 2541 3169 3547 2541
Size: 628 378 4854
Fragments arranged by size:
4854 628 378
EcoRV GAT'ATC
Cuts at: 2461 2461
Size: 5860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 5007 1678
Size: 3329 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373
Size: 32 135 32 2929 32 42 32 2626
Fragments arranged by size:
2929 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 2359 3185 2359
Size: 826 5034
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 10
Size: 54 1425 343 855 3183
Fragments arranged by size:
3183 1425 855 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 2631 3818 4177 4336 518
Size: 648 1255 210 1187 359 159 2042
Fragments arranged by size:
2042 1255 1187 648 359 210 159
MspA1I CmG'CkG
Cuts at: 1606 3276 3953 5101 5346 1606
Size: 1670 677 1148 245 2120
Fragments arranged by size:
2120 1670 1148 677 245
NaeI GCC'GGC
Cuts at: 2784 2784
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4369
Fragments arranged by size:
4369 939 552
NdeI CA'TA_TG
Cuts at: 387 2302 387
Size: 1915 3945
NgoMIV G'CCGG_C
Cuts at: 2782 2782
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2158 3050 3072 3218 4763 1132
Size: 97 929 892 22 146 1545 2229
Fragments arranged by size:
2229 1545 929 892 146 97 22
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2303 1108
Size: 1195 4665
PfoI T'CCnGG_A
Cuts at: 2040 2536 2040
Size: 496 5364
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2331 2363 2331
Size: 32 5828
PstI C_TGCA'G
Cuts at: 830 2057 2895 3212 830
Size: 1227 838 317 3478
Fragments arranged by size:
3478 1227 838 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2053 2891 3208 4343 5024 5215 826
Size: 219 1008 838 317 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 838 681 317 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2238 3964 4865 5127 820
Size: 197 926 295 1726 901 262 1553
Fragments arranged by size:
1726 1553 926 901 295 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 2344 152
Size: 2192 3668
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 3299 513
Size: 552 939 1295 3074
Fragments arranged by size:
3074 1295 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2338 2526 4106
Size: 274 80 33 51 1443 361 188 1580
Cuts at: 4106 5818 96
Size: 1712 138
Fragments arranged by size:
1712 1580 1443 361 274 188 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3048 3080 5820 5852 3048
Size: 32 2740 32 3056
Fragments arranged by size:
3056 2740 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 2712 2659
Size: 53 5807
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI