Restriction map for
pFN21AE3988
(Circular) (Six-base) MAPSORT of: pFN21AE3988.seq Check: 375 from: 1 to: 5689
pFN21AE3988 5689 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 3032 1937
Size: 475 620 4594
Fragments arranged by size:
4594 620 475
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2350 3752 5136 5404 1632
Size: 718 1402 1384 268 1917
Fragments arranged by size:
1917 1402 1384 718 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2777 2998 3224 3376 5485 1493
Size: 1284 221 226 152 2109 1697
Fragments arranged by size:
2109 1697 1284 226 221 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2733 3008 1391
Size: 1342 275 4072
Fragments arranged by size:
4072 1342 275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 928
Size: 971 204 4514
Fragments arranged by size:
4514 971 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 2859 1548
Size: 1311 4378
BfrBI ATG'CAT
Cuts at: 2450 2450
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2601 5684 2601
Size: 3083 2606
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2623 2089
Size: 534 5155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 4349 1239
Size: 827 2283 2579
Fragments arranged by size:
2579 2283 827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2161 3480 493
Size: 711 957 1319 2702
Fragments arranged by size:
2702 1319 957 711
BsaBI GATnn'nnATC
Cuts at: 2174 5683 2174
Size: 3509 2180
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 1178
Size: 30 165 30 708 30 4726
Fragments arranged by size:
4726 708 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2706 1163
Size: 573 970 4146
Fragments arranged by size:
4146 970 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2733 3008 3750 3835 4906 1391
Size: 399 943 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 943 742 399 275 85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2773 3578 5308 2773
Size: 805 1730 3154
Fragments arranged by size:
3154 1730 805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 3044 844
Size: 1274 926 3489
Fragments arranged by size:
3489 1274 926
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2196 4184 4358 65
Size: 1183 948 1988 174 1396
Fragments arranged by size:
1988 1396 1183 948 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2649 4333 4520 1700
Size: 110 228 611 1684 187 2869
Fragments arranged by size:
2869 1684 611 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 2127 96
Size: 2031 3658
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2254 1184
Size: 1070 4619
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2161
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2601 3019 3386 3771 3788 5240 5326 5338
Size: 629 418 367 385 17 1452 86 12
Cuts at: 5338 5684 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 629 418 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2532 513
Size: 552 939 6 522 3670
Fragments arranged by size:
3670 939 552 522 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2236 3136
Size: 54 1425 50 275 18 177 229 900
Cuts at: 3136 4025 5654 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 900 889 275 229 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 2846 3619 1353
Size: 1016 477 773 3423
Fragments arranged by size:
3423 1016 773 477
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2350 3752 4349 5136 5404 1239
Size: 393 434 284 1402 597 787 268 1524
Fragments arranged by size:
1524 1402 787 597 434 393 284 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2114
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2050 2050
Size: 5689
EcoRI G'AATT_C
Cuts at: 2777 2998 3376 2777
Size: 221 378 5090
Fragments arranged by size:
5090 378 221
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2039 4836 1678
Size: 361 2797 2531
Fragments arranged by size:
2797 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2851 2883 4330 4362 4404
Size: 32 135 32 1279 32 1447 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1447 1279 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2084 748
Size: 1336 4353
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3647 4006 4165 518
Size: 648 993 1488 359 159 2042
Fragments arranged by size:
2042 1488 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 3105 3782 4930 5175 1606
Size: 406 1093 677 1148 245 2120
Fragments arranged by size:
2120 1148 1093 677 406 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2532 513
Size: 552 939 528 3670
Fragments arranged by size:
3670 939 552 528
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NsiI A_TGCA'T
Cuts at: 2452 2452
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2871 1348
Size: 1523 4166
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PmlI CAC'GTG
Cuts at: 2161 2161
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2095 2115 3041 830
Size: 1265 20 926 3478
Fragments arranged by size:
3478 1265 926 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2091 2111 2628 2638 3037 4172 4853
Size: 219 1046 20 517 10 399 1135 681
Cuts at: 4853 5044 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1046 681 517 399 219 191
20 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 2038 1700
Size: 338 5351
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 3128 513
Size: 552 939 528 596 3074
Fragments arranged by size:
3074 939 596 552 528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 3935 5647
Size: 274 80 33 51 1443 150 1808 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1808 1712 1443 274 150 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI