Restriction map for
pFN21AE3987
(Circular) (Six-base) MAPSORT of: pFN21AE3987.seq Check: 9215 from: 1 to: 5683
pFN21AE3987 5683 bp
With 182 enzymes: SgfI *
March 28, 2012 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2517 2517
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 3026 1937
Size: 208 881 4594
Fragments arranged by size:
4594 881 208
Acc65I G'GTAC_C
Cuts at: 2558 3004 2558
Size: 446 5237
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 3746 5130 5398 1632
Size: 704 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1384 704 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2140 4582 5559 1203
Size: 937 2442 977 1327
Fragments arranged by size:
2442 1327 977 937
AgeI A'CCGG_T
Cuts at: 2124 4514 2124
Size: 2390 3293
AhdI GACnn_n'nnGTC
Cuts at: 2174 4412 2174
Size: 2238 3445
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2094 2518 2878 3426 4998 2094
Size: 424 360 548 1572 2779
Fragments arranged by size:
2779 1572 548 424 360
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 3008 1870
Size: 73 99 966 4545
Fragments arranged by size:
4545 966 99 73
BamHI G'GATC_C
Cuts at: 2076 3013 3380 2076
Size: 937 367 4379
Fragments arranged by size:
4379 937 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 2353 2558 3004 4459
Size: 325 217 627 397 169 205 446 1455
Cuts at: 4459 618
Size: 1842
Fragments arranged by size:
1842 1455 627 446 397 325 217 205
169
BanII G_rGCy'C
Cuts at: 1391 2293 2607 3002 1391
Size: 902 314 395 4072
Fragments arranged by size:
4072 902 395 314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2174 2208 3874 3908 1903
Size: 34 237 34 1666 34 3678
Fragments arranged by size:
3678 1666 237 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BfrBI ATG'CAT
Cuts at: 2469 2469
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2046 2409 5678 2046
Size: 363 3269 2051
Fragments arranged by size:
3269 2051 363
BlpI GC'TnA_GC
Cuts at: 2606 3100 2606
Size: 494 5189
Bme1580I G_kGCm'C
Cuts at: 1790 2309 2358 3744 4900 1790
Size: 519 49 1386 1156 2573
Fragments arranged by size:
2573 1386 1156 519 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 3414 4372
Size: 416 53 83 399 24 843 1154 958
Cuts at: 4372 442
Size: 1753
Fragments arranged by size:
1753 1154 958 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2411 2768 2786 4343 1239
Size: 832 340 357 18 1557 2579
Fragments arranged by size:
2579 1557 832 357 340 18
Bpu10I CC'TnA_GC
Cuts at: 2345 2345
Size: 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2401 2645 2885 3808 4673 4971 2401
Size: 244 240 923 865 298 3113
Fragments arranged by size:
3113 923 865 298 244 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 2081 5677 2081
Size: 3596 2087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2073 2124 4116 4514 4788 4935 5346 2073
Size: 51 1992 398 274 147 411 2410
Fragments arranged by size:
2410 1992 411 398 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 1178
Size: 30 165 30 1356 30 4072
Fragments arranged by size:
4072 1356 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2821 1186
Size: 1635 4048
BseYI C'CCAG_C
Cuts at: 2372 2551 2632 2878 4886 5382 2372
Size: 179 81 246 2008 496 2673
Fragments arranged by size:
2673 2008 496 246 179 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2623 1163
Size: 573 645 242 4223
Fragments arranged by size:
4223 645 573 242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 3133 3894 4922 1540
Size: 275 186 124 1008 761 1028 2301
Fragments arranged by size:
2301 1028 1008 761 275 186 124
BsiHKAI G_wGCw'C
Cuts at: 2508 2607 3744 3829 4900 2508
Size: 99 1137 85 1071 3291
Fragments arranged by size:
3291 1137 1071 99 85
BsmI GAATG_Cn'
Cuts at: 2471 2760 3194 3287 2471
Size: 289 434 93 4867
Fragments arranged by size:
4867 434 289 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2293 2309 2358 2508 2607 3002 3744
Size: 399 503 16 49 150 99 395 742
Cuts at: 3744 3829 4900 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 503 399 395 150 99
85 49 16
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3038 844
Size: 1673 521 3489
Fragments arranged by size:
3489 1673 521
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2124 2758 4327 4514 1700
Size: 110 314 634 1569 187 2869
Fragments arranged by size:
2869 1569 634 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 2194 4487 2194
Size: 2293 3390
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2046 2076 2409 2700 2907 3013 3380 3765
Size: 74 30 333 291 207 106 367 385
Cuts at: 3765 3782 5234 5320 5332 5678 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 333 291 207
106 86 74 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2415 513
Size: 552 939 411 3781
Fragments arranged by size:
3781 939 552 411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2337 2667 3130
Size: 54 1425 50 275 18 507 330 463
Cuts at: 3130 4019 5648 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 507 463 330 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 3613 1353
Size: 749 1511 3423
Fragments arranged by size:
3423 1511 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2915 4270 4644 4790 387
Size: 1064 138 1326 1355 374 146 1280
Fragments arranged by size:
1355 1326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2605 2605
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2336 2411 2768 2786 3746 4343
Size: 393 439 265 75 357 18 960 597
Cuts at: 4343 5130 5398 1239
Size: 787 268 1524
Fragments arranged by size:
1524 960 787 597 439 393 357 268
265 75 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2915 2915
Size: 5683
EcoO109I rG'GnC_Cy
Cuts at: 2260 2385 2440 2515 2719 2786 2260
Size: 125 55 75 204 67 5157
Fragments arranged by size:
5157 204 125 75 67 55
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 2696 4189 2696
Size: 1493 4190
HaeII r_GCGC'y
Cuts at: 1678 2323 4830 1678
Size: 645 2507 2531
Fragments arranged by size:
2531 2507 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 4324 4356 4398
Size: 32 135 32 588 32 2132 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 2132 588 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 3027 3273 669
Size: 988 281 208 881 246 3079
Fragments arranged by size:
3079 988 881 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 2562 3008 2562
Size: 446 5237
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 3024
Fragments arranged by size:
3024 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2403 3099 3776 4924 5169 1606
Size: 497 300 696 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 497 300 245
NaeI GCC'GGC
Cuts at: 2760 2760
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 2004 2415 513
Size: 552 939 411 3781
Fragments arranged by size:
3781 939 552 411
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2758 2758
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NsiI A_TGCA'T
Cuts at: 2471 2471
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2144 2336 3041 4586 1132
Size: 97 915 192 705 1545 2229
Fragments arranged by size:
2229 1545 915 705 192 97
PasI CC'CwG_GG
Cuts at: 1348 2293 1348
Size: 945 4738
PciI A'CATG_T
Cuts at: 2140 4582 2140
Size: 2442 3241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2421 2935 1108
Size: 1313 514 3856
Fragments arranged by size:
3856 1313 514
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2385 2440 2786 2385
Size: 55 346 5282
Fragments arranged by size:
5282 346 55
PshAI GACnn'nnGTC
Cuts at: 1613 2015 1613
Size: 402 5281
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2514 2601 2619 2784 2874 3035 830
Size: 1684 87 18 165 90 161 3478
Fragments arranged by size:
3478 1684 165 161 90 87 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2403 2403
Size: 5683
SacI G_AGCT'C
Cuts at: 2607 2607
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 2144 3025 1936
Size: 208 881 4594
Fragments arranged by size:
4594 881 208
SanDI GG'GwC_CC
Cuts at: 2786 2786
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 2514 2601 2784 3035 2514
Size: 87 183 251 5162
Fragments arranged by size:
5162 251 183 87
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2510 2597 2615 2780 2870 3031 4166
Size: 219 1465 87 18 165 90 161 1135
Cuts at: 4166 4847 5038 826
Size: 681 191 1471
Fragments arranged by size:
1471 1465 1135 681 219 191 165 161
90 87 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2380 2624 2864 3787 4688
Size: 197 926 99 338 244 240 923 901
Cuts at: 4688 4950 820
Size: 262 1553
Fragments arranged by size:
1553 926 923 901 338 262 244 240
197 99
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2004 2415 3122 513
Size: 552 939 411 707 3074
Fragments arranged by size:
3074 939 707 552 411
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2222 2543 2588 3710 3727 3880
Size: 1255 443 199 321 45 1122 17 153
Cuts at: 3880 4065 4510 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1122 445 443 321 199 185
153 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2759 2791 5643 5675 2111
Size: 32 616 32 2852 32 2119
Fragments arranged by size:
2852 2119 616 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2033 2483 1316
Size: 717 450 4516
Fragments arranged by size:
4516 717 450
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2965 2011
Size: 954 4729
XhoI C'TCGA_G
Cuts at: 1943 2042 1943
Size: 99 5584
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NarI NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI