Restriction map for
pFN21AE3985
(Circular) (Six-base) MAPSORT of: pFN21AE3985.seq Check: 7649 from: 1 to: 5872
pFN21AE3985 5872 bp
With 182 enzymes: SgfI *
March 28, 2012 11:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2662 3935 5319 5587 1632
Size: 1030 1273 1384 268 1917
Fragments arranged by size:
1917 1384 1273 1030 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 2358 3153 4771 5748 1203
Size: 1155 795 1618 977 1327
Fragments arranged by size:
1618 1327 1155 977 795
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 2126 2324 1166
Size: 960 198 4714
Fragments arranged by size:
4714 960 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2785 1184
Size: 1601 4271
AlwNI CAG_nnn'CTG
Cuts at: 3047 3615 5187 3047
Size: 568 1572 3732
Fragments arranged by size:
3732 1572 568
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2572 2615 3181 3407 3559 5668 1493
Size: 1079 43 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 566 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 2493 4426 160
Size: 872 1461 1933 1606
Fragments arranged by size:
1933 1606 1461 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 3197 1870
Size: 73 1254 4545
Fragments arranged by size:
4545 1254 73
BamHI G'GATC_C
Cuts at: 2893 3202 3569 2893
Size: 309 367 5196
Fragments arranged by size:
5196 367 309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 4648 618
Size: 325 217 627 1406 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3191 1391
Size: 1800 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 4063 4097 1903
Size: 34 152 34 1940 34 3678
Fragments arranged by size:
3678 1940 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 2169 1548
Size: 621 5251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 3933 5089 1790
Size: 2143 1156 2573
Fragments arranged by size:
2573 2143 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2636 1056
Size: 1580 4292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2813
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 4532 1239
Size: 1574 1719 2579
Fragments arranged by size:
2579 1719 1574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4862 5160 3997
Size: 865 298 4709
Fragments arranged by size:
4709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2584 4305 4703 4977 5124 5535 2584
Size: 1721 398 274 147 411 2921
Fragments arranged by size:
2921 1721 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5647
Fragments arranged by size:
5647 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2418 3042 1186
Size: 1232 624 4016
Fragments arranged by size:
4016 1232 624
BseYI C'CCAG_C
Cuts at: 5075 5571 5075
Size: 496 5376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2321 3933 4018 5089 2321
Size: 1612 85 1071 3104
Fragments arranged by size:
3104 1612 1071 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 3191 3933 4018 5089 1391
Size: 399 531 870 742 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 531 399 85
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4367 4541 65
Size: 1183 3119 174 1396
Fragments arranged by size:
3119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 2439 4676 2439
Size: 2237 3635
BstEII G'GTnAC_C
Cuts at: 1184 2014 1184
Size: 830 5042
BstYI r'GATC_y
Cuts at: 1972 2893 3202 3569 3954 3971 5423 5509 5521
Size: 921 309 367 385 17 1452 86 12
Cuts at: 5521 5867 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 921 385 367 346 309 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2244 2244
Size: 5872
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5320
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2674 487
Size: 683 56 1448 3685
Fragments arranged by size:
3685 1448 683 56
BtsI GCAGTG_nn'
Cuts at: 3388 4182 4202 3388
Size: 794 20 5058
Fragments arranged by size:
5058 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DraIII CAC_nnn'GTG
Cuts at: 3164 3164
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2375 4879 809
Size: 507 1059 2504 1802
Fragments arranged by size:
2504 1802 1059 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2730 3319
Size: 54 1425 50 275 18 264 636 589
Cuts at: 3319 4208 5837 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 636 589 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3126 3802 1353
Size: 1773 676 3423
Fragments arranged by size:
3423 1773 676
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2662 2813 3935 4532 5319 5587 1239
Size: 393 1030 151 1122 597 787 268 1524
Fragments arranged by size:
1524 1122 1030 787 597 393 268 151
EcoRI G'AATT_C
Cuts at: 2572 3181 3559 2572
Size: 609 378 4885
Fragments arranged by size:
4885 609 378
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 5019 1678
Size: 3341 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2269 2666 3132 4986 5170 1305
Size: 964 397 466 1854 184 2007
Fragments arranged by size:
2007 1854 964 466 397 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2732 10
Size: 54 1425 343 264 636 3150
Fragments arranged by size:
3150 1425 636 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2324 3830 4189 4348 518
Size: 648 960 198 1506 359 159 2042
Fragments arranged by size:
2042 1506 960 648 359 198 159
MspA1I CmG'CkG
Cuts at: 1606 2745 3288 3965 5113 5358 1606
Size: 1139 543 677 1148 245 2120
Fragments arranged by size:
2120 1148 1139 677 543 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5320
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 2632 1052
Size: 1580 4292
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2362 2762 3157 3230 4775 1132
Size: 97 1133 400 395 73 1545 2229
Fragments arranged by size:
2229 1545 1133 400 395 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5872
PciI A'CATG_T
Cuts at: 2358 3153 4771 2358
Size: 795 1618 3459
Fragments arranged by size:
3459 1618 795
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2424 1613
Size: 811 5061
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 2435 4120 5832 2435
Size: 1685 1712 2475
Fragments arranged by size:
2475 1712 1685
SfcI C'TryA_G
Cuts at: 826 1045 3220 4355 5036 5227 826
Size: 219 2175 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3976 4877 5139 820
Size: 197 926 2033 901 262 1553
Fragments arranged by size:
2033 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 3311 513
Size: 552 2246 3074
Fragments arranged by size:
3074 2246 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 2492 3063 3899 3916 4069 4254
Size: 1255 856 56 571 836 17 153 185
Cuts at: 4254 4699 325
Size: 445 1498
Fragments arranged by size:
1498 1255 856 836 571 445 185 153
56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 4118 5830
Size: 274 80 33 51 1443 456 1685 1712
Cuts at: 5830 96
Size: 138
Fragments arranged by size:
1712 1685 1443 456 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5832 5864 5832
Size: 32 5840
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI