Restriction map for
pFN21AE3973
(Circular) (Six-base) MAPSORT of: pFN21AE3973.seq Check: 8438 from: 1 to: 6007
pFN21AE3973 6007 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2155 2155
Size: 6007
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 2045 4070 5454 5722 1632
Size: 363 50 2025 1384 268 1917
Fragments arranged by size:
2025 1917 1384 363 268 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 2932 4906 5883 1203
Size: 1729 1974 977 1327
Fragments arranged by size:
1974 1729 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 2862 1166
Size: 1696 4311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 2450 3750 5322 2450
Size: 1300 1572 3135
Fragments arranged by size:
3135 1572 1300
ApaLI G'TGCA_C
Cuts at: 3200 4064 5220 3200
Size: 864 1156 3987
Fragments arranged by size:
3987 1156 864
ApoI r'AATT_y
Cuts at: 1493 2165 3316 3542 3694 5803 1493
Size: 672 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2816 3332 1870
Size: 73 873 516 4545
Fragments arranged by size:
4545 873 516 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3328 4783 618
Size: 325 217 627 1541 1455 1842
Fragments arranged by size:
1842 1541 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3326 1391
Size: 1935 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4198 4232 1903
Size: 34 50 34 2177 34 3678
Fragments arranged by size:
3678 2177 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6002 6002
Size: 6007
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 3204 4068 5224 1790
Size: 1414 864 1156 2573
Fragments arranged by size:
2573 1414 1156 864
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3738 4696 442
Size: 416 53 83 399 24 2321 958 1753
Fragments arranged by size:
2321 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4667 1239
Size: 3428 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3221 4670 882
Size: 617 1722 1449 2219
Fragments arranged by size:
2219 1722 1449 617
BsaAI yAC'GTr
Cuts at: 493 1204 3205 3798 493
Size: 711 2001 593 2702
Fragments arranged by size:
2702 2001 711 593
BsaBI GATnn'nnATC
Cuts at: 2123 6001 2123
Size: 3878 2129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3814 4196 4831 275
Size: 53 83 186 936 2281 382 635 1451
Fragments arranged by size:
2281 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2547 2577 3200 3230 1178
Size: 30 165 30 1144 30 623 30 3955
Fragments arranged by size:
3955 1144 623 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2761 1186
Size: 1575 4432
BseYI C'CCAG_C
Cuts at: 5210 5706 5210
Size: 496 5511
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 2813 2840 1163
Size: 573 906 171 27 4330
Fragments arranged by size:
4330 906 573 171 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2753 3204 4068 4153 5224 2753
Size: 451 864 85 1071 3536
Fragments arranged by size:
3536 1071 864 451 85
BsmI GAATG_Cn'
Cuts at: 2572 3518 3611 2572
Size: 946 93 4968
Fragments arranged by size:
4968 946 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2753 3204 3326 4068 4153 5224 1391
Size: 399 963 451 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 963 742 451 399 122 85
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2155 3362 844
Size: 1311 1207 3489
Fragments arranged by size:
3489 1311 1207
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 3004 4067 5079 3004
Size: 1063 1012 3932
Fragments arranged by size:
3932 1063 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6007
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 3293 3293
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2646 3337 3704 4089 4106 5558 5644 5656
Size: 674 691 367 385 17 1452 86 12
Cuts at: 5656 6002 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 691 674 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 2958 3311 2958
Size: 353 5654
DraIII CAC_nnn'GTG
Cuts at: 2740 2740
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2474 3454 4343
Size: 54 1425 50 275 18 644 980 889
Cuts at: 4343 5972 8
Size: 1629 43
Fragments arranged by size:
1629 1425 980 889 644 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2906 3190 3937 1353
Size: 1553 284 747 3423
Fragments arranged by size:
3423 1553 747 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2045 4070 4667 5454 5722 1239
Size: 393 363 50 2025 597 787 268 1524
Fragments arranged by size:
2025 1524 787 597 393 363 268 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2316 2316
Size: 6007
EcoO109I rG'GnC_Cy
Cuts at: 2344 2344
Size: 6007
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3257 3257
Size: 6007
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2086 5154 1678
Size: 408 3068 2531
Fragments arranged by size:
3068 2531 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373
Size: 32 135 32 3076 32 42 32 2626
Fragments arranged by size:
3076 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 2496 3250 3351 3597 669
Size: 988 281 370 188 754 101 246 3079
Fragments arranged by size:
3079 988 754 370 281 246 188 101
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5121 5305 1305
Size: 3816 184 2007
Fragments arranged by size:
3816 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2476 10
Size: 54 1425 343 644 3541
Fragments arranged by size:
3541 1425 644 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2862 3965 4324 4483 518
Size: 648 1696 1103 359 159 2042
Fragments arranged by size:
2042 1696 1103 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2862 3423 4100 5248 5493 1606
Size: 1256 561 677 1148 245 2120
Fragments arranged by size:
2120 1256 1148 677 561 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5455
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2420 2936 3365 4910 1132
Size: 97 1191 516 429 1545 2229
Fragments arranged by size:
2229 1545 1191 516 429 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 2932 4906 2932
Size: 1974 4033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PmlI CAC'GTG
Cuts at: 3205 3205
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 6007
SfcI C'TryA_G
Cuts at: 826 1045 3355 4490 5171 5362 826
Size: 219 2310 1135 681 191 1471
Fragments arranged by size:
2310 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 2355 2920 3931 5
Size: 47 2303 565 1011 2081
Fragments arranged by size:
2303 2081 1011 565 47
StuI AGG'CCT
Cuts at: 1517 2322 1517
Size: 805 5202
StyI C'CwwG_G
Cuts at: 513 1065 3446 513
Size: 552 2381 3074
Fragments arranged by size:
3074 2381 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3245 4034 4051 4204 4389 4834 325
Size: 1255 1665 789 17 153 185 445 1498
Fragments arranged by size:
1665 1498 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2063 2589 4253
Size: 274 80 33 51 1443 86 526 1664
Cuts at: 4253 5965 96
Size: 1712 138
Fragments arranged by size:
1712 1664 1443 526 274 138 86 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2308 2340 3200 3232 5967 5999 2308
Size: 32 860 32 2735 32 2316
Fragments arranged by size:
2735 2316 860 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI