Restriction map for
pFN21AE3965
(Circular) (Six-base) MAPSORT of: pFN21AE3965.seq Check: 1790 from: 1 to: 5701
pFN21AE3965 5701 bp
With 182 enzymes: SgfI *
March 28, 2012 11:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2341 2718 3764 5148 5416 1632
Size: 585 124 377 1046 1384 268 1917
Fragments arranged by size:
1917 1384 1046 585 377 268 124
AfeI AGC'GCT
Cuts at: 2529 2529
Size: 5701
AflII C'TTAA_G
Cuts at: 820 1017 2678 820
Size: 197 1661 3843
Fragments arranged by size:
3843 1661 197
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 2140 2562 3010 3236 3388 5497 1493
Size: 647 422 448 226 152 2109 1697
Fragments arranged by size:
2109 1697 647 448 422 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 1870
Size: 73 1083 4545
Fragments arranged by size:
4545 1083 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3022 4477 618
Size: 325 217 627 1235 1455 1842
Fragments arranged by size:
1842 1455 1235 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3020 1391
Size: 1629 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2493 2999 928
Size: 971 594 506 3630
Fragments arranged by size:
3630 971 594 506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4312 136
Size: 107 122 71 631 605 2640 1525
Fragments arranged by size:
2640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2507 5696 2507
Size: 3189 2512
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2921 3762 4918 1790
Size: 1131 841 1156 2573
Fragments arranged by size:
2573 1156 1131 841
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 2311 2863
Size: 416 53 83 399 24 732 162 552
Cuts at: 2863 3432 4390 442
Size: 569 958 1753
Fragments arranged by size:
1753 958 732 569 552 416 399 162
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4361 1239
Size: 3122 2579
Bpu10I CC'TnA_GC
Cuts at: 2539 2539
Size: 5701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2643 3826 4691 4989 2643
Size: 1183 865 298 3355
Fragments arranged by size:
3355 1183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 4364 882
Size: 617 645 2220 2219
Fragments arranged by size:
2220 2219 645 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3508 3890 4525 275
Size: 53 83 186 936 1975 382 635 1451
Fragments arranged by size:
1975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5701
BseYI C'CCAG_C
Cuts at: 4904 5400 4904
Size: 496 5205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2947 1163
Size: 573 1211 3917
Fragments arranged by size:
3917 1211 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2921 3020 3762 3847 4918 1391
Size: 399 1131 99 742 85 1071 2174
Fragments arranged by size:
2174 1131 1071 742 399 99 85
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 2671 3590 5320 2671
Size: 919 1730 3052
Fragments arranged by size:
3052 1730 919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2081 3056 844
Size: 1237 975 3489
Fragments arranged by size:
3489 1237 975
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 4196 4370 65
Size: 1183 1069 1879 174 1396
Fragments arranged by size:
1879 1396 1183 1069 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 4345 4532 1700
Size: 110 280 2255 187 2869
Fragments arranged by size:
2869 2255 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2095
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 2507 3031 3398 3783 3800 5252 5338 5350
Size: 535 524 367 385 17 1452 86 12
Cuts at: 5350 5696 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 535 524 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 513
Size: 552 939 576 3634
Fragments arranged by size:
3634 939 576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 487
Size: 683 56 815 4147
Fragments arranged by size:
4147 815 683 56
BtsI GCAGTG_nn'
Cuts at: 2921 3217 4011 4031 2921
Size: 296 794 20 4591
Fragments arranged by size:
4591 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3148 4037 5666
Size: 54 1425 50 275 18 1318 889 1629
Cuts at: 5666 8
Size: 43
Fragments arranged by size:
1629 1425 1318 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2737 2802 3631 1353
Size: 789 595 65 829 3423
Fragments arranged by size:
3423 829 789 595 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2341 2718 3764 4361 5148 5416
Size: 393 585 124 377 1046 597 787 268
Cuts at: 5416 1239
Size: 1524
Fragments arranged by size:
1524 1046 787 597 585 393 377 268
124
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2531 4848 1678
Size: 853 2317 2531
Fragments arranged by size:
2531 2317 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 4342 4374 4416
Size: 32 135 32 637 32 2101 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 2101 637 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2745 4815 4999 1305
Size: 1440 2070 184 2007
Fragments arranged by size:
2070 2007 1440 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3879
Fragments arranged by size:
3879 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2884 3659 4018 4177 518
Size: 648 894 824 775 359 159 2042
Fragments arranged by size:
2042 894 824 775 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3117 3794 4942 5187 1606
Size: 1511 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2580 513
Size: 552 939 576 3634
Fragments arranged by size:
3634 939 576 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2298 2348 2883 3059 4604 1132
Size: 97 1069 50 535 176 1545 2229
Fragments arranged by size:
2229 1545 1069 535 176 97 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 3770 3802 5283 5315 2141
Size: 32 1597 32 1481 32 2527
Fragments arranged by size:
2527 1597 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 2616 3271 2616
Size: 655 5046
PstI C_TGCA'G
Cuts at: 830 2340 2630 3053 830
Size: 1510 290 423 3478
Fragments arranged by size:
3478 1510 423 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2336 2626 3049 4184 4865 5056 826
Size: 219 1291 290 423 1135 681 191 1471
Fragments arranged by size:
1471 1291 1135 681 423 290 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2658 2678 3805 4706 4968 820
Size: 197 926 715 20 1127 901 262 1553
Fragments arranged by size:
1553 1127 926 901 715 262 197 20
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 2634 2645 3625 5
Size: 47 2582 11 980 2081
Fragments arranged by size:
2582 2081 980 47 11
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 2004 2580 3140 513
Size: 552 939 576 560 3074
Fragments arranged by size:
3074 939 576 560 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3728 3745 3898 4083 4528 325
Size: 1255 2148 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3947 5659 96
Size: 274 80 33 51 1443 1970 1712 138
Fragments arranged by size:
1970 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI