Restriction map for
pFN21AE3964
(Circular) (Six-base) MAPSORT of: pFN21AE3964.seq Check: 3526 from: 1 to: 5620
pFN21AE3964 5620 bp
With 182 enzymes: SgfI *
March 28, 2012 11:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 2715 3747 4120 1563
Size: 1152 1032 373 3063
Fragments arranged by size:
3063 1152 1032 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2613 3683 5067 5335 1632
Size: 981 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1070 981 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 2321 2365 3363 4935 2321
Size: 44 998 1572 3006
Fragments arranged by size:
3006 1572 998 44
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2450 2895 2929 3155 3307 5416 1493
Size: 957 445 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 957 445 226 152 34
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 2818 1548
Size: 1270 4350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2675 4231 136
Size: 107 122 71 631 605 1003 1556 1525
Fragments arranged by size:
1556 1525 1003 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2649 5615 2649
Size: 2966 2654
BlpI GC'TnA_GC
Cuts at: 2687 3037 2687
Size: 350 5270
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2353 3351 4309
Size: 416 53 83 399 24 936 998 958
Cuts at: 4309 442
Size: 1753
Fragments arranged by size:
1753 998 958 936 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 4280 1239
Size: 965 2076 2579
Fragments arranged by size:
2579 2076 965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 2197 2610 4823 5319 2197
Size: 413 2213 496 2498
Fragments arranged by size:
2498 2213 496 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3681 3766 4837 3681
Size: 85 1071 4464
Fragments arranged by size:
4464 1071 85
BsmI GAATG_Cn'
Cuts at: 2720 3131 3224 2720
Size: 411 93 5116
Fragments arranged by size:
5116 411 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2939 3681 3766 4837 1391
Size: 399 1149 742 85 1071 2174
Fragments arranged by size:
2174 1149 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2691 3507 1394
Size: 429 868 816 3507
Fragments arranged by size:
3507 868 816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2235 4115 4289 65
Size: 1183 987 1880 174 1396
Fragments arranged by size:
1880 1396 1183 987 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 2659 96
Size: 2563 3057
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 2440 3680 4692 2440
Size: 1240 1012 3368
Fragments arranged by size:
3368 1240 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2649 2950 3317 3702 3719 5171 5257 5269
Size: 677 301 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 677 385 367 346 301 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5068
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2622 3136 3930 3950 2622
Size: 514 794 20 4292
Fragments arranged by size:
4292 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 2692 3067
Size: 54 1425 50 275 18 537 325 375
Cuts at: 3067 3956 5585 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 537 375 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 3550 1353
Size: 680 1517 3423
Fragments arranged by size:
3423 1517 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2613 3683 4280 5067 5335 1239
Size: 393 572 409 1070 597 787 268 1524
Fragments arranged by size:
1524 1070 787 597 572 409 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2135
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2318 2318
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4261 4293 4335 4367 1373
Size: 32 135 32 2689 32 42 32 2626
Fragments arranged by size:
2689 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 2916 3219 2916
Size: 303 5317
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2767 2860 4734 4918 1305
Size: 1462 93 1874 184 2007
Fragments arranged by size:
2007 1874 1462 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 10
Size: 54 1425 343 537 3261
Fragments arranged by size:
3261 1425 537 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2052 2904 3578 3937 4096 518
Size: 648 886 852 674 359 159 2042
Fragments arranged by size:
2042 886 852 674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 3036 3713 4861 5106 1606
Size: 591 839 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 677 591 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5068
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2669 2978 4523 1132
Size: 97 1440 309 1545 2229
Fragments arranged by size:
2229 1545 1440 309 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2365 1108
Size: 1257 4363
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2318 2318
Size: 5620
PshAI GACnn'nnGTC
Cuts at: 1613 2108 1613
Size: 495 5125
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2197 2197
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SexAI A'CCwGG_T
Cuts at: 2130 2130
Size: 5620
SfcI C'TryA_G
Cuts at: 826 1045 2171 2968 4103 4784 4975 826
Size: 219 1126 797 1135 681 191 1471
Fragments arranged by size:
1471 1135 1126 797 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 2429 2524 3544 5
Size: 47 2377 95 1020 2081
Fragments arranged by size:
2377 2081 1020 95 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 3059 513
Size: 552 1994 3074
Fragments arranged by size:
3074 1994 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2695 3647 3664 3817 4002 4447 325
Size: 1255 1115 952 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1115 952 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2659 3866 5578
Size: 274 80 33 51 1443 682 1207 1712
Cuts at: 5578 96
Size: 138
Fragments arranged by size:
1712 1443 1207 682 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2159 2169 2191 2732 2764 5580 5612 2137
Size: 22 10 22 541 32 2816 32 2145
Fragments arranged by size:
2816 2145 541 32 32 22 22 10
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 2860 3749 2860
Size: 889 4731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI