Restriction map for
pFN21AE3960
(Circular) (Six-base) MAPSORT of: pFN21AE3960.seq Check: 1798 from: 1 to: 6391
pFN21AE3960 6391 bp
With 182 enzymes: SgfI *
March 28, 2012 11:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3670 3734 1937
Size: 1733 64 4594
Fragments arranged by size:
4594 1733 64
Acc65I G'GTAC_C
Cuts at: 3549 3712 3549
Size: 163 6228
AclI AA'CG_TT
Cuts at: 1563 3264 4518 4891 1563
Size: 1701 1254 373 3063
Fragments arranged by size:
3063 1701 1254 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 4454 5838 6106 1632
Size: 1280 1542 1384 268 1917
Fragments arranged by size:
1917 1542 1384 1280 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 2156 5290 6267 1203
Size: 953 3134 977 1327
Fragments arranged by size:
3134 1327 977 953
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 2522 2673 1166
Size: 1356 151 4884
Fragments arranged by size:
4884 1356 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3166 1184
Size: 1982 4409
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3249 3281 3249
Size: 32 6359
AlwNI CAG_nnn'CTG
Cuts at: 2401 2965 4134 5706 2401
Size: 564 1169 1572 3086
Fragments arranged by size:
3086 1572 1169 564
ApaI G_GGCC'C
Cuts at: 2917 2917
Size: 6391
ApaLI G'TGCA_C
Cuts at: 4448 5604 4448
Size: 1156 5235
ApoI r'AATT_y
Cuts at: 1493 2926 3165 3614 3700 3926 4078 6187 1493
Size: 1433 239 449 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1433 449 239 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3186 3716 1870
Size: 73 1243 530 4545
Fragments arranged by size:
4545 1243 530 73
AvrII C'CTAG_G
Cuts at: 3553 3553
Size: 6391
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3533 3549 3712 5167 618
Size: 325 217 627 1746 16 163 1455 1842
Fragments arranged by size:
1842 1746 1455 627 325 217 163 16
BanII G_rGCy'C
Cuts at: 1391 2178 2727 2917 3187 3422 3710 1391
Size: 787 549 190 270 235 288 4072
Fragments arranged by size:
4072 787 549 288 270 235 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2910 928
Size: 971 1011 4409
Fragments arranged by size:
4409 1011 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4582 4616 1903
Size: 34 2645 34 3678
Fragments arranged by size:
3678 2645 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4283 5493 4283
Size: 1210 5181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5002 136
Size: 107 122 71 631 605 3330 1525
Fragments arranged by size:
3330 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6386 6386
Size: 6391
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 2800 2917 4452 5608 1790
Size: 1010 117 1535 1156 2573
Fragments arranged by size:
2573 1535 1156 1010 117
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3299 4122 5080
Size: 416 53 83 399 24 1882 823 958
Cuts at: 5080 442
Size: 1753
Fragments arranged by size:
1882 1753 958 823 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2192 2393 2780 2786 3003 3123 3478
Size: 944 9 201 387 6 217 120 355
Cuts at: 3478 5051 1239
Size: 1573 2579
Fragments arranged by size:
2579 1573 944 387 355 217 201 120
9 6
Bpu10I CC'TnA_GC
Cuts at: 2301 3065 2301
Size: 764 5627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 4516 5381 5679 2231
Size: 2285 865 298 2943
Fragments arranged by size:
2943 2285 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5054 882
Size: 617 3555 2219
Fragments arranged by size:
3555 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2157 2384 2749 4182 493
Size: 711 953 227 365 1433 2702
Fragments arranged by size:
2702 1433 953 711 365 227
BsaBI GATnn'nnATC
Cuts at: 2276 3035 6385 2276
Size: 759 3350 2282
Fragments arranged by size:
3350 2282 759
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3375 4198 4580 5215
Size: 53 83 186 936 1842 823 382 635
Cuts at: 5215 275
Size: 1451
Fragments arranged by size:
1842 1451 936 823 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2835 4824 5222 5496 5643 6054 2835
Size: 1989 398 274 147 411 3172
Fragments arranged by size:
3172 1989 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3249 3279 1178
Size: 30 165 30 1846 30 4290
Fragments arranged by size:
4290 1846 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2717 2885 3270 3457 3679 1186
Size: 1531 168 385 187 222 3898
Fragments arranged by size:
3898 1531 385 222 187 168
BseYI C'CCAG_C
Cuts at: 2029 2949 5594 6090 2029
Size: 920 2645 496 2330
Fragments arranged by size:
2645 2330 920 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 3434 3466 1163
Size: 573 310 1388 32 4088
Fragments arranged by size:
4088 1388 573 310 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3841 4602 5630 1540
Size: 275 186 1840 761 1028 2301
Fragments arranged by size:
2301 1840 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2727 3003 4452 4537 5608 2727
Size: 276 1449 85 1071 3510
Fragments arranged by size:
3510 1449 1071 276 85
BsmI GAATG_Cn'
Cuts at: 3638 3902 3995 3638
Size: 264 93 6034
Fragments arranged by size:
6034 264 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2727 2800 2917 3003 3187 3422
Size: 399 388 549 73 117 86 184 235
Cuts at: 3422 3710 4452 4537 5608 1391
Size: 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 399 388 288 235
184 117 86 85 73
BspEI T'CCGG_A
Cuts at: 2835 6054 2835
Size: 3219 3172
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2459 3746 844
Size: 1475 140 1287 3489
Fragments arranged by size:
3489 1475 1287 140
BsrBI CCG'CTC
Cuts at: 1394 1823 3515 4278 1394
Size: 429 1692 763 3507
Fragments arranged by size:
3507 1692 763 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 4886 5060 65
Size: 1183 1447 2191 174 1396
Fragments arranged by size:
2191 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2625 5035 5222 1700
Size: 110 815 2410 187 2869
Fragments arranged by size:
2869 2410 815 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3489 794
Size: 2695 3696
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2705 3049 3155 2161
Size: 544 344 106 5397
Fragments arranged by size:
5397 544 344 106
BstYI r'GATC_y
Cuts at: 1972 2778 3030 3721 4088 4473 4490 5942 6028
Size: 806 252 691 367 385 17 1452 86
Cuts at: 6028 6040 6386 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 806 691 385 367 346 252
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3132 3132
Size: 6391
BtgI C'CryG_G
Cuts at: 513 1065 2316 2520 3154 3565 513
Size: 552 1251 204 634 411 3339
Fragments arranged by size:
3339 1251 634 552 411 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5652
Fragments arranged by size:
5652 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2826 3475 3907 4701 4721 2086
Size: 740 649 432 794 20 3756
Fragments arranged by size:
3756 794 740 649 432 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DraIII CAC_nnn'GTG
Cuts at: 2157 2157
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3305 5398 809
Size: 507 1989 2093 1802
Fragments arranged by size:
2093 1989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 3562 3838
Size: 54 1425 50 275 18 522 1210 276
Cuts at: 3838 4727 6356 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1210 889 522 276 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 4321 1353
Size: 1079 1889 3423
Fragments arranged by size:
3423 1889 1079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4978 5352 5498 387
Size: 1064 138 3389 374 146 1280
Fragments arranged by size:
3389 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2725 2725
Size: 6391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2192 2393 2780 2786 2912 3003
Size: 393 551 9 201 387 6 126 91
Cuts at: 3003 3123 3478 4454 5051 5838 6106 1239
Size: 120 355 976 597 787 268 1524
Fragments arranged by size:
1524 976 787 597 551 393 387 355
268 201 126 120 91 9 6
EcoO109I rG'GnC_Cy
Cuts at: 2398 2755 2398
Size: 357 6034
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 6391
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 5538 1678
Size: 3860 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 3670 3702 5032
Size: 32 135 32 632 32 1434 32 1330
Cuts at: 5032 5064 5106 5138 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1434 1330 632 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3735 3981 669
Size: 988 281 1797 246 3079
Fragments arranged by size:
3079 1797 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KpnI G_GTAC'C
Cuts at: 3553 3716 3553
Size: 163 6228
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5505 5689 1305
Size: 4200 184 2007
Fragments arranged by size:
4200 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3564 10
Size: 54 1425 343 1732 2837
Fragments arranged by size:
2837 1732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 2673 2703 4349 4708 4867 518
Size: 648 1356 151 30 1646 359 159 2042
Fragments arranged by size:
2042 1646 1356 648 359 159 151 30
MspA1I CmG'CkG
Cuts at: 1606 2170 2828 2899 2938 2953 3015 3498 3807
Size: 564 658 71 39 15 62 483 309
Cuts at: 3807 4484 5632 5877 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 658 564 483 309 245
71 62 39 15
NcoI C'CATG_G
Cuts at: 513 1065 2316 2520 3565 513
Size: 552 1251 204 1045 3339
Fragments arranged by size:
3339 1251 1045 552 204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 2977 3413 3749 5294 1132
Size: 97 1748 436 336 1545 2229
Fragments arranged by size:
2229 1748 1545 436 336 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 2521 1108
Size: 1293 120 4978
Fragments arranged by size:
4978 1293 120
PfoI T'CCnGG_A
Cuts at: 2238 3100 3321 2238
Size: 862 221 5308
Fragments arranged by size:
5308 862 221
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PmlI CAC'GTG
Cuts at: 2157 2749 2157
Size: 592 5799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3249 3281 4460 4492 5973 6005 3249
Size: 32 1179 32 1481 32 3635
Fragments arranged by size:
3635 1481 1179 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PspOMI G'GGCC_C
Cuts at: 2913 2913
Size: 6391
PstI C_TGCA'G
Cuts at: 830 2265 2593 2937 2943 3743 830
Size: 1435 328 344 6 800 3478
Fragments arranged by size:
3478 1435 800 344 328 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 2170 2828 2899 2938 2953 2170
Size: 658 71 39 15 5608
Fragments arranged by size:
5608 658 71 39 15
SacI G_AGCT'C
Cuts at: 2727 2727
Size: 6391
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SfcI C'TryA_G
Cuts at: 826 1045 2261 2548 2589 2933 2939 3739 4874
Size: 219 1216 287 41 344 6 800 1135
Cuts at: 4874 5555 5746 826
Size: 681 191 1471
Fragments arranged by size:
1471 1216 1135 800 681 344 287 219
191 41 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3188 3718 1872
Size: 1316 530 4545
Fragments arranged by size:
4545 1316 530
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 4495 5396 5658 820
Size: 197 926 303 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 2051 152
Size: 1899 4492
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SspI AAT'ATT
Cuts at: 5 52 2235 4315 5
Size: 47 2183 2080 2081
Fragments arranged by size:
2183 2081 2080 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2316 2520 3363 3553 3565 3830 513
Size: 552 1251 204 843 190 12 265 3074
Fragments arranged by size:
3074 1251 843 552 265 204 190 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2309 3597 4418 4435 4588 4773 5218
Size: 1255 729 1288 821 17 153 185 445
Cuts at: 5218 325
Size: 1498
Fragments arranged by size:
1498 1288 1255 821 729 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4637 6349 96
Size: 274 80 33 51 1443 2660 1712 138
Fragments arranged by size:
2660 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2702 2734 6351 6383 2702
Size: 32 3617 32 2710
Fragments arranged by size:
3617 2710 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 3049 2347
Size: 702 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3186 3716 1870
Size: 1316 530 4545
Fragments arranged by size:
4545 1316 530
XmnI GAAnn'nnTTC
Cuts at: 4520 4520
Size: 6391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI