Restriction map for
pFN21AE3954
(Circular) (Six-base) MAPSORT of: pFN21AE3954.seq Check: 1026 from: 1 to: 6553
pFN21AE3954 6553 bp
With 182 enzymes: SgfI *
March 28, 2012 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2385 2957 3691 2385
Size: 572 734 5247
Fragments arranged by size:
5247 734 572
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2148 4363 278
Size: 53 83 186 936 612 2215 2468
Fragments arranged by size:
2468 2215 936 612 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2070 2400 2589 3741 3896 1937
Size: 133 330 189 1152 155 4594
Fragments arranged by size:
4594 1152 330 189 155 133
Acc65I G'GTAC_C
Cuts at: 3874 3874
Size: 6553
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3225 4616 6000 6268 1632
Size: 1593 1391 1384 268 1917
Fragments arranged by size:
1917 1593 1391 1384 268
AfeI AGC'GCT
Cuts at: 3234 3533 3234
Size: 299 6254
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6356
AflIII A'CryG_T
Cuts at: 1203 3417 5452 6429 1203
Size: 2214 2035 977 1327
Fragments arranged by size:
2214 2035 1327 977
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 3255 3274 5282 3255
Size: 19 2008 4526
Fragments arranged by size:
4526 2008 19
AleI CACnn'nnGTG
Cuts at: 1166 3018 1166
Size: 1852 4701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2117 2624 2888 3407 1184
Size: 933 507 264 519 4330
Fragments arranged by size:
4330 933 519 507 264
AlwNI CAG_nnn'CTG
Cuts at: 2110 2464 2581 2656 2698 4296 5868 2110
Size: 354 117 75 42 1598 1572 2795
Fragments arranged by size:
2795 1598 1572 354 117 75 42
ApaLI G'TGCA_C
Cuts at: 4610 5766 4610
Size: 1156 5397
ApoI r'AATT_y
Cuts at: 1493 3862 4088 4240 6349 1493
Size: 2369 226 152 2109 1697
Fragments arranged by size:
2369 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 3349 3878 1870
Size: 73 726 680 529 4545
Fragments arranged by size:
4545 726 680 529 73
BamHI G'GATC_C
Cuts at: 3883 4250 3883
Size: 367 6186
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 3320 3592 3834 3874
Size: 325 217 627 489 1044 272 242 40
Cuts at: 3874 5329 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1044 627 489 325 272 242
217 40
BanII G_rGCy'C
Cuts at: 1391 2136 2295 2937 3872 1391
Size: 745 159 642 935 4072
Fragments arranged by size:
4072 935 745 642 159
BbeI G_GCGC'C
Cuts at: 2280 3596 2280
Size: 1316 5237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3198 928
Size: 971 1299 4283
Fragments arranged by size:
4283 1299 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 4744 4778 1903
Size: 34 1784 34 989 34 3678
Fragments arranged by size:
3678 1784 989 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4445 5655 4445
Size: 1210 5343
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6553
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3004 3318 5164
Size: 107 122 71 631 605 1332 314 1846
Cuts at: 5164 136
Size: 1525
Fragments arranged by size:
1846 1525 1332 631 605 314 122 107
71
BglII A'GATC_T
Cuts at: 2385 6548 2385
Size: 4163 2390
BlpI GC'TnA_GC
Cuts at: 3970 3970
Size: 6553
Bme1580I G_kGCm'C
Cuts at: 1790 3323 4614 5770 1790
Size: 1533 1291 1156 2573
Fragments arranged by size:
2573 1533 1291 1156
BmgBI CAC'GTC
Cuts at: 1318 3418 3541 1318
Size: 2100 123 4330
Fragments arranged by size:
4330 2100 123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4284 5242 442
Size: 416 53 83 399 24 2867 958 1753
Fragments arranged by size:
2867 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2441 2720 2951 3809 5213 1239
Size: 1145 57 279 231 858 1404 2579
Fragments arranged by size:
2579 1404 1145 858 279 231 57
Bpu10I CC'TnA_GC
Cuts at: 2594 2594
Size: 6553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2279 2468 4678 5543 5841 2074
Size: 205 189 2210 865 298 2786
Fragments arranged by size:
2786 2210 865 298 205 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2920 5216 882
Size: 617 1421 2296 2219
Fragments arranged by size:
2296 2219 1421 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 4344 493
Size: 711 2102 1038 2702
Fragments arranged by size:
2702 2102 1038 711
BsaBI GATnn'nnATC
Cuts at: 6547 6547
Size: 6553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2277 2961 2990
Size: 53 83 186 936 612 132 684 29
Cuts at: 2990 3593 3738 4360 4742 5377 275
Size: 603 145 622 382 635 1451
Fragments arranged by size:
1451 936 684 635 622 612 603 382
186 145 132 83 53 29
BsaWI w'CCGG_w
Cuts at: 3408 4986 5384 5658 5805 6216 3408
Size: 1578 398 274 147 411 3745
Fragments arranged by size:
3745 1578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 3167 3197 1178
Size: 30 165 30 801 30 933 30 4534
Fragments arranged by size:
4534 933 801 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2822 3440 1186
Size: 1636 618 4299
Fragments arranged by size:
4299 1636 618
BseYI C'CCAG_C
Cuts at: 2076 2841 3031 3544 3669 5756 6252 2076
Size: 765 190 513 125 2087 496 2377
Fragments arranged by size:
2377 2087 765 513 496 190 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2224 2630 2726 3490 3719 1163
Size: 573 488 406 96 764 229 3997
Fragments arranged by size:
3997 764 573 488 406 229 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2760 3217 3712 4003 4764 5792
Size: 275 186 759 457 495 291 761 1028
Cuts at: 5792 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 759 495 457 291 275
186
BsiHKAI G_wGCw'C
Cuts at: 2628 2768 2937 4614 4699 5770 2628
Size: 140 169 1677 85 1071 3411
Fragments arranged by size:
3411 1677 1071 169 140 85
BsiWI C'GTAC_G
Cuts at: 3306 3306
Size: 6553
BsmI GAATG_Cn'
Cuts at: 4064 4157 4064
Size: 93 6460
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 2360 2572 2981 3242 2129
Size: 231 212 409 261 5440
Fragments arranged by size:
5440 409 261 231 212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2295 2628 2768 2937 3323 3872
Size: 399 346 159 333 140 169 386 549
Cuts at: 3872 4614 4699 5770 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 399 386 346 333
169 159 140 85
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 4442 6172 4442
Size: 1730 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2385 2463 2745 2957 3399 3691 3753
Size: 1355 186 78 282 212 442 292 62
Cuts at: 3753 3908 844
Size: 155 3489
Fragments arranged by size:
3489 1355 442 292 282 212 186 155
78 62
BsrBI CCG'CTC
Cuts at: 1394 1823 2719 2923 3430 4440 1394
Size: 429 896 204 507 1010 3507
Fragments arranged by size:
3507 1010 896 507 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 5048 5222 65
Size: 1183 3800 174 1396
Fragments arranged by size:
3800 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2755 3053 3287 5197 5384 1700
Size: 110 945 298 234 1910 187 2869
Fragments arranged by size:
2869 1910 945 298 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 3342 96
Size: 3246 3307
BssHII G'CGCG_C
Cuts at: 1925 3449 1925
Size: 1524 5029
BssSI C'ACGA_G
Cuts at: 4613 5625 4613
Size: 1012 5541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 794
Size: 1379 5174
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 2385 3883 4250 4635 4652 6104 6190 6202
Size: 413 1498 367 385 17 1452 86 12
Cuts at: 6202 6548 1972
Size: 346 1977
Fragments arranged by size:
1977 1498 1452 413 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2071 2071
Size: 6553
BtgI C'CryG_G
Cuts at: 513 1065 2128 2301 3444 3525 3794 513
Size: 552 1063 173 1143 81 269 3272
Fragments arranged by size:
3272 1143 1063 552 269 173 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2168 3034 3522 3742 487
Size: 683 56 942 866 488 220 3298
Fragments arranged by size:
3298 942 866 683 488 220 56
BtsI GCAGTG_nn'
Cuts at: 2657 2981 3575 3636 3678 4069 4863 4883 2657
Size: 324 594 61 42 391 794 20 4327
Fragments arranged by size:
4327 794 594 391 324 61 42 20
ClaI AT'CG_AT
Cuts at: 1554 2505 1554
Size: 951 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6518
DraI TTT'AAA
Cuts at: 3857 3857
Size: 6553
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2032 5560 809
Size: 507 716 3528 1802
Fragments arranged by size:
3528 1802 716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2039 2157 2196
Size: 54 1425 50 275 18 209 118 39
Cuts at: 2196 2757 3204 3214 3285 3310 3797 4000 4889
Size: 561 447 10 71 25 487 203 889
Cuts at: 4889 6518 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 561 487 447 275 209
203 118 71 54 50 43 39 25
18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2757 3214 4000 1537
Size: 275 945 457 786 4090
Fragments arranged by size:
4090 945 786 457 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 3244 4483 1353
Size: 788 1103 1239 3423
Fragments arranged by size:
3423 1239 1103 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3022 3760 5140 5514 5660 387
Size: 1064 138 1433 738 1380 374 146 1280
Fragments arranged by size:
1433 1380 1280 1064 738 374 146 138
EcoICRI GAG'CTC
Cuts at: 2935 2935
Size: 6553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 2441 2720 2951 3225 3809 4616
Size: 393 752 57 279 231 274 584 807
Cuts at: 4616 5213 6000 6268 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 807 787 752 597 584 393 279
274 268 231 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 2636 3749 2423
Size: 213 1113 5227
Fragments arranged by size:
5227 1113 213
EcoRI G'AATT_C
Cuts at: 3862 4240 3862
Size: 378 6175
FspI TGC'GCA
Cuts at: 2876 5059 2876
Size: 2183 4370
HaeII r_GCGC'y
Cuts at: 1678 2280 2873 2893 3163 3236 3535 3596 5700
Size: 602 593 20 270 73 299 61 2104
Cuts at: 5700 1678
Size: 2531
Fragments arranged by size:
2531 2104 602 593 299 270 73 61
20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3077 3109 3241 3260 3273
Size: 32 135 32 1505 32 132 19 13
Cuts at: 3273 3292 5194 5226 5268 5300 1373
Size: 19 1902 32 42 32 2626
Fragments arranged by size:
2626 1902 1505 135 132 42 32 32
32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3742 3897 4143 669
Size: 988 281 1804 155 246 3079
Fragments arranged by size:
3079 1804 988 281 246 155
HindIII A'AGCT_T
Cuts at: 748 2247 748
Size: 1499 5054
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KasI G'GCGC_C
Cuts at: 2276 3592 2276
Size: 1316 5237
KpnI G_GTAC'C
Cuts at: 3878 3878
Size: 6553
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5667 5851 1305
Size: 4362 184 2007
Fragments arranged by size:
4362 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3206 10
Size: 54 1425 343 1374 3357
Fragments arranged by size:
3357 1425 1374 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3018 3200 4511 4870 5029 518
Size: 648 1071 781 182 1311 359 159 2042
Fragments arranged by size:
2042 1311 1071 781 648 359 182 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2107 2130 2614 2995 3123 3598 3669
Size: 470 31 23 484 381 128 475 71
Cuts at: 3669 3969 4646 5794 6039 1606
Size: 300 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 484 475 470 381 300
245 128 71 31 23
NaeI GCC'GGC
Cuts at: 2757 3055 3289 2757
Size: 298 234 6021
Fragments arranged by size:
6021 298 234
NarI GG'CG_CC
Cuts at: 2277 3593 2277
Size: 1316 5237
NcoI C'CATG_G
Cuts at: 513 1065 3525 513
Size: 552 2460 3541
Fragments arranged by size:
3541 2460 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6553
NgoMIV G'CCGG_C
Cuts at: 2755 3053 3287 2755
Size: 298 234 6021
Fragments arranged by size:
6021 298 234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 2242 3199 3469 3608 3911 5456 1132
Size: 97 1013 957 270 139 303 1545 2229
Fragments arranged by size:
2229 1545 1013 957 303 270 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6553
PciI A'CATG_T
Cuts at: 5452 5452
Size: 6553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2698 1108
Size: 1590 4963
PfoI T'CCnGG_A
Cuts at: 2361 2361
Size: 6553
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6553
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PstI C_TGCA'G
Cuts at: 830 2106 2385 2460 2562 2640 2652 3905 830
Size: 1276 279 75 102 78 12 1253 3478
Fragments arranged by size:
3478 1276 1253 279 102 78 75 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 3669 3669
Size: 6553
SacI G_AGCT'C
Cuts at: 2937 2937
Size: 6553
SacII CC_GC'GG
Cuts at: 2131 2131
Size: 6553
SalI G'TCGA_C
Cuts at: 1936 3740 3895 1936
Size: 1804 155 4594
Fragments arranged by size:
4594 1804 155
SapI GCTCTTCn'nnn_
Cuts at: 2141 2141
Size: 6553
SbfI CC_TGCA'GG
Cuts at: 2460 2640 3905 2460
Size: 180 1265 5108
Fragments arranged by size:
5108 1265 180
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SfcI C'TryA_G
Cuts at: 826 1045 2102 2381 2456 2476 2558 2636 2648
Size: 219 1057 279 75 20 82 78 12
Cuts at: 2648 3559 3664 3901 5036 5717 5908 826
Size: 911 105 237 1135 681 191 1471
Fragments arranged by size:
1471 1135 1057 911 681 279 237 219
191 105 82 78 75 20 12
SfoI GGC'GCC
Cuts at: 2278 3594 2278
Size: 1316 5237
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 3053 1700
Size: 1353 5200
SmaI CCC'GGG
Cuts at: 1872 3880 1872
Size: 2008 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2089 2294 2483 4657 5558 5820
Size: 197 926 146 205 189 2174 901 262
Cuts at: 5820 820
Size: 1553
Fragments arranged by size:
2174 1553 926 901 262 205 197 189
146
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 2242 3608 3911 1132
Size: 1110 1366 303 3774
Fragments arranged by size:
3774 1366 1110 303
SspI AAT'ATT
Cuts at: 5 52 4477 5
Size: 47 4425 2081
Fragments arranged by size:
4425 2081 47
StuI AGG'CCT
Cuts at: 1517 3557 1517
Size: 2040 4513
StyI C'CwwG_G
Cuts at: 513 1065 2778 3296 3525 3750 3992 513
Size: 552 1713 518 229 225 242 3074
Fragments arranged by size:
3074 1713 552 518 242 229 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3467 4580 4597 4750 4935 5380 325
Size: 1255 1887 1113 17 153 185 445 1498
Fragments arranged by size:
1887 1498 1255 1113 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3342 4799 6511
Size: 274 80 33 51 1443 1365 1457 1712
Cuts at: 6511 96
Size: 138
Fragments arranged by size:
1712 1457 1443 1365 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6513 6545 6513
Size: 32 6521
Tth111I GACn'n_nGTC
Cuts at: 1316 2147 2357 1316
Size: 831 210 5512
Fragments arranged by size:
5512 831 210
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6553
XmaI C'CCGG_G
Cuts at: 1870 3878 1870
Size: 2008 4545
XmnI GAAnn'nnTTC
Cuts at: 2525 4682 2525
Size: 2157 4396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2146 4361 276
Size: 53 83 186 936 612 2215 2468
Fragments arranged by size:
2468 2215 936 612 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BstXI
Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SexAI SfiI SrfI SwaI XcmI