Restriction map for
pFN21AE3953
(Circular) (Six-base) MAPSORT of: pFN21AE3953.seq Check: 1192 from: 1 to: 5515
pFN21AE3953 5515 bp
With 182 enzymes: SgfI *
March 28, 2012 11:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 3578 4962 5230 1632
Size: 845 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 845 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2106 2224 2660 3258 4830 2106
Size: 118 436 598 1572 2791
Fragments arranged by size:
2791 1572 598 436 118
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2561 2824 3050 3202 5311 1493
Size: 1068 263 226 152 2109 1697
Fragments arranged by size:
2109 1697 1068 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 1870
Size: 73 897 4545
Fragments arranged by size:
4545 897 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2267 2703 2834 1391
Size: 876 436 131 4072
Fragments arranged by size:
4072 876 436 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2642 928
Size: 971 743 3801
Fragments arranged by size:
3801 971 743
BbvCI CC'TCA_GC
Cuts at: 2661 2661
Size: 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3407 4617 2544
Size: 863 1210 3442
Fragments arranged by size:
3442 1210 863
BclI T'GATC_A
Cuts at: 1548 2296 2566 1548
Size: 748 270 4497
Fragments arranged by size:
4497 748 270
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2287 5510 2287
Size: 3223 2292
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 3576 4732 1790
Size: 1786 1156 2573
Fragments arranged by size:
2573 1786 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2330 2722 2330
Size: 392 5123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2420 4175 1239
Size: 842 339 1755 2579
Fragments arranged by size:
2579 1755 842 339
Bpu10I CC'TnA_GC
Cuts at: 2661 2661
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 2231 2432 3640 4505 4803 2101
Size: 130 201 1208 865 298 2813
Fragments arranged by size:
2813 1208 865 298 201 130
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2373 4178 882
Size: 617 874 1805 2219
Fragments arranged by size:
2219 1805 874 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 3704 4339 275
Size: 53 83 186 936 1789 382 635 1451
Fragments arranged by size:
1789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 1178
Size: 30 165 30 840 30 4420
Fragments arranged by size:
4420 840 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2483 2609 1186
Size: 865 432 126 4092
Fragments arranged by size:
4092 865 432 126
BseYI C'CCAG_C
Cuts at: 4718 5214 4718
Size: 496 5019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2379 2703 3576 3661 4732 2379
Size: 324 873 85 1071 3162
Fragments arranged by size:
3162 1071 873 324 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2379 2703 2834 3576 3661 4732
Size: 399 477 112 324 131 742 85 1071
Cuts at: 4732 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 477 399 324 131 112
85
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 2769 3404 5134 2769
Size: 635 1730 3150
Fragments arranged by size:
3150 1730 635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2365 3402 1394
Size: 429 542 1037 3507
Fragments arranged by size:
3507 1037 542 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 2041 2384 1184
Size: 857 343 4315
Fragments arranged by size:
4315 857 343
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 2236 2059
Size: 177 5338
BstYI r'GATC_y
Cuts at: 1972 2287 2845 3212 3597 3614 5066 5152 5164
Size: 315 558 367 385 17 1452 86 12
Cuts at: 5164 5510 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 558 385 367 346 315 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2380 513
Size: 552 939 376 3648
Fragments arranged by size:
3648 939 552 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 3031 3825 3845 2148
Size: 883 794 20 3818
Fragments arranged by size:
3818 883 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2226 2790
Size: 54 1425 50 275 18 265 131 564
Cuts at: 2790 2962 3851 5480 8
Size: 172 889 1629 43
Fragments arranged by size:
1629 1425 889 564 275 265 172 131
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2327 2460 2499 3445 1353
Size: 923 51 133 39 946 3423
Fragments arranged by size:
3423 946 923 133 51 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2701 2701
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2420 2477 3578 4175 4962 5230
Size: 393 449 339 57 1101 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 1101 787 597 449 393 339 268
57
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2402
Size: 5515
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2688 2720 4156
Size: 32 135 32 531 32 553 32 1436
Cuts at: 4156 4188 4230 4262 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1436 553 531 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2070 2736 2859 3105 669
Size: 988 281 132 666 123 246 3079
Fragments arranged by size:
3079 988 666 281 246 132 123
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2702 4629 4813 1305
Size: 1397 1927 184 2007
Fragments arranged by size:
2007 1927 1397 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2097 2228 2792 10
Size: 54 1425 343 265 131 564 2733
Fragments arranged by size:
2733 1425 564 343 265 131 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3473 3832 3991 518
Size: 648 957 1350 359 159 2042
Fragments arranged by size:
2042 1350 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2169 2931 3608 4756 5001 1606
Size: 563 762 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 563 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2273 1108
Size: 1165 4350
PfoI T'CCnGG_A
Cuts at: 2516 2516
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2754 2867 830
Size: 1924 113 3478
Fragments arranged by size:
3478 1924 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2169 2169
Size: 5515
SacI G_AGCT'C
Cuts at: 2703 2703
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2207 2750 2863 3998 4679 4870 826
Size: 219 1162 543 113 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 681 543 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 2246 2447 3619 4520 4782
Size: 197 926 137 166 201 1172 901 262
Cuts at: 4782 820
Size: 1553
Fragments arranged by size:
1553 1172 926 901 262 201 197 166
137
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2954 513
Size: 552 939 950 3074
Fragments arranged by size:
3074 950 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 3542 3559 3712 3897 4342 325
Size: 1255 1196 766 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1196 766 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2086 2851 2086
Size: 765 4750
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI