Restriction map for
pFN21AE3951
(Circular) (Six-base) MAPSORT of: pFN21AE3951.seq Check: 2047 from: 1 to: 6976
pFN21AE3951 6976 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4786 278
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4319 1937
Size: 2382 4594
Acc65I G'GTAC_C
Cuts at: 4297 4297
Size: 6976
AclI AA'CG_TT
Cuts at: 1563 5103 5476 1563
Size: 3540 373 3063
Fragments arranged by size:
3540 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5039 6423 6691 1632
Size: 3407 1384 268 1917
Fragments arranged by size:
3407 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6779
AflIII A'CryG_T
Cuts at: 1203 2487 3528 5875 6852 1203
Size: 1284 1041 2347 977 1327
Fragments arranged by size:
2347 1327 1284 1041 977
AgeI A'CCGG_T
Cuts at: 5807 5807
Size: 6976
AhdI GACnn_n'nnGTC
Cuts at: 5705 5705
Size: 6976
AleI CACnn'nnGTG
Cuts at: 1166 3020 1166
Size: 1854 5122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2148 1184
Size: 964 6012
AlwNI CAG_nnn'CTG
Cuts at: 3667 4013 4719 6291 3667
Size: 346 706 1572 4352
Fragments arranged by size:
4352 1572 706 346
ApaI G_GGCC'C
Cuts at: 2168 2187 2499 3855 2168
Size: 19 312 1356 5289
Fragments arranged by size:
5289 1356 312 19
ApaLI G'TGCA_C
Cuts at: 5033 6189 5033
Size: 1156 5820
ApoI r'AATT_y
Cuts at: 1493 4285 4511 4663 6772 1493
Size: 2792 226 152 2109 1697
Fragments arranged by size:
2792 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2197 2197
Size: 6976
AseI AT'TA_AT
Cuts at: 160 1032 5530 160
Size: 872 4498 1606
Fragments arranged by size:
4498 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2186 2213 2363 2392 2425 2993
Size: 73 144 99 27 150 29 33 568
Cuts at: 2993 3185 3199 3866 4301 1870
Size: 192 14 667 435 4545
Fragments arranged by size:
4545 667 568 435 192 150 144 99
73 33 29 27 14
BamHI G'GATC_C
Cuts at: 4000 4306 4673 4000
Size: 306 367 6303
Fragments arranged by size:
6303 367 306
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2068 2173 2396 2421
Size: 325 217 627 253 28 105 223 25
Cuts at: 2421 2614 2630 2657 2762 2811 2998 3422 4297
Size: 193 16 27 105 49 187 424 875
Cuts at: 4297 5752 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 875 627 424 325 253 223
217 193 187 105 105 49 28 27
25 16
BanII G_rGCy'C
Cuts at: 1391 2015 2168 2187 2364 2499 2517 2730 3042
Size: 624 153 19 177 135 18 213 312
Cuts at: 3042 3449 3716 3855 4079 4295 1391
Size: 407 267 139 224 216 4072
Fragments arranged by size:
4072 624 407 312 267 224 216 213
177 153 139 135 19 18
BbeI G_GCGC'C
Cuts at: 2044 2072 2400 2618 2634 2661 2766 2815 3426
Size: 28 328 218 16 27 105 49 611
Cuts at: 3426 2044
Size: 5594
Fragments arranged by size:
5594 611 328 218 105 49 28 27
16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 4074 928
Size: 971 526 1649 3830
Fragments arranged by size:
3830 1649 971 526
BbvCI CC'TCA_GC
Cuts at: 2589 3206 2589
Size: 617 6359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5167 5201 1903
Size: 34 50 34 3146 34 3678
Fragments arranged by size:
3678 3146 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4868 6078 4868
Size: 1210 5766
BclI T'GATC_A
Cuts at: 1548 2277 1548
Size: 729 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3201 5587 136
Size: 107 122 71 631 605 1529 2386 1525
Fragments arranged by size:
2386 1529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6971 6971
Size: 6976
BlpI GC'TnA_GC
Cuts at: 2009 3411 4393 2009
Size: 1402 982 4592
Fragments arranged by size:
4592 1402 982
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2187 2499 3001 3577 3612 3855 5037
Size: 378 19 312 502 576 35 243 1182
Cuts at: 5037 6193 1790
Size: 1156 2573
Fragments arranged by size:
2573 1182 1156 576 502 378 312 243
35 19
BmgBI CAC'GTC
Cuts at: 1318 2488 2545 3229 3543 1318
Size: 1170 57 684 314 4751
Fragments arranged by size:
4751 1170 684 314 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4707 5665 442
Size: 416 53 83 399 24 3290 958 1753
Fragments arranged by size:
3290 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5636 1239
Size: 4397 2579
Bpu10I CC'TnA_GC
Cuts at: 2589 3206 3875 3951 2589
Size: 617 669 76 5614
Fragments arranged by size:
5614 669 617 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 5101 5966 6264 2714
Size: 2387 865 298 3426
Fragments arranged by size:
3426 2387 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 5639 882
Size: 617 1032 3108 2219
Fragments arranged by size:
3108 2219 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 2443 3055 4767 493
Size: 711 1239 612 1712 2702
Fragments arranged by size:
2702 1712 1239 711 612
BsaBI GATnn'nnATC
Cuts at: 2276 3968 6970 2276
Size: 1692 3002 2282
Fragments arranged by size:
3002 2282 1692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 2069 2397 2615
Size: 53 83 186 936 508 28 328 218
Cuts at: 2615 2631 2658 2763 2812 3423 4783 5165 5800
Size: 16 27 105 49 611 1360 382 635
Cuts at: 5800 275
Size: 1451
Fragments arranged by size:
1451 1360 936 635 611 508 382 328
218 186 105 83 53 49 28 27
16
BsaWI w'CCGG_w
Cuts at: 3004 5409 5807 6081 6228 6639 3004
Size: 2405 398 274 147 411 3341
Fragments arranged by size:
3341 2405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3050 3080 3309 3339 4168
Size: 30 165 30 1647 30 229 30 829
Cuts at: 4168 4198 1178
Size: 30 3956
Fragments arranged by size:
3956 1647 829 229 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 2525 2606 3906 3971 4121 1186
Size: 1336 3 81 1300 65 150 4041
Fragments arranged by size:
4041 1336 1300 150 81 65 3
BseYI C'CCAG_C
Cuts at: 2479 3232 3313 3844 3895 4036 6179 6675 2479
Size: 753 81 531 51 141 2143 496 2780
Fragments arranged by size:
2780 2143 753 531 496 141 81 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 1163
Size: 573 720 5683
Fragments arranged by size:
5683 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4426 5187 6215 1540
Size: 275 186 2425 761 1028 2301
Fragments arranged by size:
2425 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2015 2448 2517 2730 3384 4186 5037 5122 6193
Size: 433 69 213 654 802 851 85 1071
Cuts at: 6193 2015
Size: 2798
Fragments arranged by size:
2798 1071 851 802 654 433 213 85
69
BsiWI C'GTAC_G
Cuts at: 2439 2439
Size: 6976
BsmI GAATG_Cn'
Cuts at: 4487 4580 4487
Size: 93 6883
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2297 3026 2029
Size: 268 729 5979
Fragments arranged by size:
5979 729 268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2168 2187 2364 2448 2499 2517
Size: 399 225 153 19 177 84 51 18
Cuts at: 2517 2730 3001 3042 3384 3449 3577 3612 3716
Size: 213 271 41 342 65 128 35 104
Cuts at: 3716 3855 4079 4186 4295 5037 5122 6193 1391
Size: 139 224 107 109 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 271 225 224
213 177 153 139 128 109 107 104
85 84 65 51 41 35 19 18
BspEI T'CCGG_A
Cuts at: 3004 6639 3004
Size: 3635 3341
BspHI T'CATG_A
Cuts at: 4865 6595 4865
Size: 1730 5246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4331 844
Size: 3487 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2313 2717 3887 4863 1394
Size: 429 490 404 1170 976 3507
Fragments arranged by size:
3507 1170 976 490 429 404
BsrDI GCAATG_nn'
Cuts at: 65 1248 5471 5645 65
Size: 1183 4223 174 1396
Fragments arranged by size:
4223 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2193 2221 2348 2418 2462 2571
Size: 110 255 128 28 127 70 44 109
Cuts at: 2571 5620 5807 1700
Size: 3049 187 2869
Fragments arranged by size:
3049 2869 255 187 128 127 110 109
70 44 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6976
BssHII G'CGCG_C
Cuts at: 1925 2197 1925
Size: 272 6704
BssSI C'ACGA_G
Cuts at: 4025 5036 6048 4025
Size: 1011 1012 4953
Fragments arranged by size:
4953 1012 1011
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3667 4013 794
Size: 2873 346 3757
Fragments arranged by size:
3757 2873 346
BstBI TT'CG_AA
Cuts at: 5780 5780
Size: 6976
BstEII G'GTnAC_C
Cuts at: 1184 2971 3499 1184
Size: 1787 528 4661
Fragments arranged by size:
4661 1787 528
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2878
Size: 6976
BstYI r'GATC_y
Cuts at: 1972 3982 4000 4306 4673 5058 5075 6527 6613
Size: 2010 18 306 367 385 17 1452 86
Cuts at: 6613 6625 6971 1972
Size: 12 346 1977
Fragments arranged by size:
2010 1977 1452 385 367 346 306 86
18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2035 2179 2232 2625 3651 3769 3816
Size: 552 970 144 53 393 1026 118 47
Cuts at: 3816 4096 4257 513
Size: 280 161 3232
Fragments arranged by size:
3232 1026 970 552 393 280 161 144
118 53 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2564 3580 487
Size: 683 56 1338 1016 3883
Fragments arranged by size:
3883 1338 1016 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 4190 4492 5286 5306 2473
Size: 1717 302 794 20 4143
Fragments arranged by size:
4143 1717 794 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6941
DraI TTT'AAA
Cuts at: 4280 4280
Size: 6976
DraIII CAC_nnn'GTG
Cuts at: 2808 3298 2808
Size: 490 6486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5983 809
Size: 507 4667 1802
Fragments arranged by size:
4667 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2346 3165
Size: 54 1425 50 275 18 399 117 819
Cuts at: 3165 3648 3654 3831 4099 4423 5312 6941 8
Size: 483 6 177 268 324 889 1629 43
Fragments arranged by size:
1629 1425 889 819 483 399 324 275
268 177 117 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4423 1537
Size: 275 2611 4090
Fragments arranged by size:
4090 2611 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2846 2900 4906 1353
Size: 1493 54 2006 3423
Fragments arranged by size:
3423 2006 1493 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5563 5937 6083 387
Size: 1064 138 3974 374 146 1280
Fragments arranged by size:
3974 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2013 2515 2728 2013
Size: 502 213 6261
Fragments arranged by size:
6261 502 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 5039 5636 6423 6691 1239
Size: 393 3407 597 787 268 1524
Fragments arranged by size:
3407 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2504
Size: 6976
EcoO109I rG'GnC_Cy
Cuts at: 2165 2496 2848 3837 3851 3852 4220 2165
Size: 331 352 989 14 1 368 4921
Fragments arranged by size:
4921 989 368 352 331 14 1
EcoRI G'AATT_C
Cuts at: 4285 4663 4285
Size: 378 6598
EcoRV GAT'ATC
Cuts at: 4269 4269
Size: 6976
FseI GG_CCGG'CC
Cuts at: 2352 2352
Size: 6976
FspI TGC'GCA
Cuts at: 2136 2324 3101 3272 5482 2136
Size: 188 777 171 2210 3630
Fragments arranged by size:
3630 2210 777 188 171
FspAI rTGC'GCAy
Cuts at: 3101 3101
Size: 6976
HaeII r_GCGC'y
Cuts at: 1678 2044 2072 2400 2600 2618 2634 2661 2737
Size: 366 28 328 200 18 16 27 76
Cuts at: 2737 2766 2815 2840 3426 3569 6123 1678
Size: 29 49 25 586 143 2554 2531
Fragments arranged by size:
2554 2531 586 366 328 200 143 76
49 29 28 27 25 18 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2840 2872 3309 3341 5617
Size: 32 135 32 1268 32 437 32 2276
Cuts at: 5617 5649 5691 5723 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2276 1268 437 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3622 4320 4566 669
Size: 988 281 1684 698 246 3079
Fragments arranged by size:
3079 1684 988 698 281 246
HindIII A'AGCT_T
Cuts at: 748 4228 748
Size: 3480 3496
HpaI GTT'AAC
Cuts at: 4566 4566
Size: 6976
KasI G'GCGC_C
Cuts at: 2040 2068 2396 2614 2630 2657 2762 2811 3422
Size: 28 328 218 16 27 105 49 611
Cuts at: 3422 2040
Size: 5594
Fragments arranged by size:
5594 611 328 218 105 49 28 27
16
KpnI G_GTAC'C
Cuts at: 4301 4301
Size: 6976
MfeI C'AATT_G
Cuts at: 4575 4575
Size: 6976
MluI A'CGCG_T
Cuts at: 6852 6852
Size: 6976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3474 6090 6274 1305
Size: 2169 2616 184 2007
Fragments arranged by size:
2616 2169 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3167 3650 3656 10
Size: 54 1425 343 1335 483 6 3330
Fragments arranged by size:
3330 1425 1335 483 343 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3599 3605 3821 4934 5293 5452
Size: 648 1854 579 6 216 1113 359 159
Cuts at: 5452 518
Size: 2042
Fragments arranged by size:
2042 1854 1113 648 579 359 216 159
6
MspA1I CmG'CkG
Cuts at: 1606 2037 2110 2181 2265 2627 2817 3777 3848
Size: 431 73 71 84 362 190 960 71
Cuts at: 3848 4013 4259 4392 5069 6217 6462 1606
Size: 165 246 133 677 1148 245 2120
Fragments arranged by size:
2120 1148 960 677 431 362 246 245
190 165 133 84 73 71 71
NaeI GCC'GGC
Cuts at: 2067 2223 2350 2067
Size: 156 127 6693
Fragments arranged by size:
6693 156 127
NarI GG'CG_CC
Cuts at: 2041 2069 2397 2615 2631 2658 2763 2812 3423
Size: 28 328 218 16 27 105 49 611
Cuts at: 3423 2041
Size: 5594
Fragments arranged by size:
5594 611 328 218 105 49 28 27
16
NcoI C'CATG_G
Cuts at: 513 1065 2232 3651 3816 513
Size: 552 1167 1419 165 3673
Fragments arranged by size:
3673 1419 1167 552 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6976
NgoMIV G'CCGG_C
Cuts at: 2065 2221 2348 2065
Size: 156 127 6693
Fragments arranged by size:
6693 156 127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6976
NotI GC'GGCC_GC
Cuts at: 4423 4423
Size: 6976
NruI TCG'CGA
Cuts at: 2583 2583
Size: 6976
NspI r_CATG'y
Cuts at: 1132 1229 3532 3637 4334 5879 1132
Size: 97 2303 105 697 1545 2229
Fragments arranged by size:
2303 2229 1545 697 105 97
PasI CC'CwG_GG
Cuts at: 1348 3373 3514 1348
Size: 2025 141 4810
Fragments arranged by size:
4810 2025 141
PciI A'CATG_T
Cuts at: 3528 5875 3528
Size: 2347 4629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3543 1108
Size: 2435 4541
PfoI T'CCnGG_A
Cuts at: 2745 2745
Size: 6976
PmeI GTTT'AAAC
Cuts at: 4280 4280
Size: 6976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5045 5077 6558 6590 5045
Size: 32 1481 32 5431
Fragments arranged by size:
5431 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2848 2848
Size: 6976
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6976
PsiI TTA'TAA
Cuts at: 4546 4546
Size: 6976
PspOMI G'GGCC_C
Cuts at: 2164 2183 2495 3851 2164
Size: 19 312 1356 5289
Fragments arranged by size:
5289 1356 312 19
PstI C_TGCA'G
Cuts at: 830 4328 830
Size: 3498 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6976
PvuII CAG'CTG
Cuts at: 4013 4013
Size: 6976
SacI G_AGCT'C
Cuts at: 2015 2517 2730 2015
Size: 502 213 6261
Fragments arranged by size:
6261 502 213
SacII CC_GC'GG
Cuts at: 2038 2182 2628 4260 2038
Size: 144 446 1632 4754
Fragments arranged by size:
4754 1632 446 144
SalI G'TCGA_C
Cuts at: 1936 4318 1936
Size: 2382 4594
SapI GCTCTTCn'nnn_
Cuts at: 2846 2846
Size: 6976
SbfI CC_TGCA'GG
Cuts at: 4328 4328
Size: 6976
ScaI AGT'ACT
Cuts at: 5224 6936 5224
Size: 1712 5264
SfcI C'TryA_G
Cuts at: 826 1045 4105 4324 5459 6140 6331 826
Size: 219 3060 219 1135 681 191 1471
Fragments arranged by size:
3060 1471 1135 681 219 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3201 3201
Size: 6976
SfoI GGC'GCC
Cuts at: 2042 2070 2398 2616 2632 2659 2764 2813 3424
Size: 28 328 218 16 27 105 49 611
Cuts at: 3424 2042
Size: 5594
Fragments arranged by size:
5594 611 328 218 105 49 28 27
16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6976
SmaI CCC'GGG
Cuts at: 1872 2188 2215 2394 2427 2995 3868 4303 1872
Size: 316 27 179 33 568 873 435 4545
Fragments arranged by size:
4545 873 568 435 316 179 33 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3185 5080 5981 6243 820
Size: 197 926 786 456 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 786 456 262 197
SnaBI TAC'GTA
Cuts at: 493 4767 493
Size: 4274 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6976
SphI G_CATG'C
Cuts at: 1132 4334 1132
Size: 3202 3774
SspI AAT'ATT
Cuts at: 5 52 4900 5
Size: 47 4848 2081
Fragments arranged by size:
4848 2081 47
StuI AGG'CCT
Cuts at: 1517 2552 2673 2981 1517
Size: 1035 121 308 5512
Fragments arranged by size:
5512 1035 308 121
StyI C'CwwG_G
Cuts at: 513 1065 2232 3213 3651 3816 4415 513
Size: 552 1167 981 438 165 599 3074
Fragments arranged by size:
3074 1167 981 599 552 438 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2555 3814 4131 5003 5020 5173 5358
Size: 1255 975 1259 317 872 17 153 185
Cuts at: 5358 5803 325
Size: 445 1498
Fragments arranged by size:
1498 1259 1255 975 872 445 317 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3636 5222 6934
Size: 274 80 33 51 1443 1659 1586 1712
Cuts at: 6934 96
Size: 138
Fragments arranged by size:
1712 1659 1586 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 4168 4200 6936 6968 3050
Size: 32 1086 32 2736 32 3058
Fragments arranged by size:
3058 2736 1086 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3544 1316
Size: 2228 4748
XbaI T'CTAG_A
Cuts at: 4312 4312
Size: 6976
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2926 3025 3229 3511 2926
Size: 99 204 282 6391
Fragments arranged by size:
6391 282 204 99
XhoI C'TCGA_G
Cuts at: 1943 3185 1943
Size: 1242 5734
XmaI C'CCGG_G
Cuts at: 1870 2186 2213 2392 2425 2993 3866 4301 1870
Size: 316 27 179 33 568 873 435 4545
Fragments arranged by size:
4545 873 568 435 316 179 33 27
XmnI GAAnn'nnTTC
Cuts at: 5105 5105
Size: 6976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4784 276
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BfrBI BplI BstZ17I
Bsu36I FalI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SexAI SrfI SwaI