Restriction map for
pFN21AE3948
(Circular) (Six-base) MAPSORT of: pFN21AE3948.seq Check: 5450 from: 1 to: 5782
pFN21AE3948 5782 bp
With 182 enzymes: SgfI *
March 28, 2012 11:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2608 2747 3125 1937
Size: 671 139 378 4594
Fragments arranged by size:
4594 671 378 139
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3081 3909 4282 1563
Size: 1518 828 373 3063
Fragments arranged by size:
3063 1518 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2732 3845 5229 5497 1632
Size: 467 633 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 633 467 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2934 3107 1870
Size: 73 991 173 4545
Fragments arranged by size:
4545 991 173 73
AvrII C'CTAG_G
Cuts at: 2551 2551
Size: 5782
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 3101 1391
Size: 632 1078 4072
Fragments arranged by size:
4072 1078 632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2912 928
Size: 971 1013 3798
Fragments arranged by size:
3798 1013 971
BbvCI CC'TCA_GC
Cuts at: 2582 2582
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2203 5777 2203
Size: 3574 2208
BlpI GC'TnA_GC
Cuts at: 2010 2562 3199 2010
Size: 552 637 4593
Fragments arranged by size:
4593 637 552
Bme1580I G_kGCm'C
Cuts at: 1790 2918 3843 4999 1790
Size: 1128 925 1156 2573
Fragments arranged by size:
2573 1156 1128 925
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2127 2127
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2414 2613 2657 4442 1239
Size: 959 216 199 44 1785 2579
Fragments arranged by size:
2579 1785 959 216 199 44
Bpu10I CC'TnA_GC
Cuts at: 2582 2652 2582
Size: 70 5712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2434 3907 4772 5070 2434
Size: 1473 865 298 3146
Fragments arranged by size:
3146 1473 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2098 2330 4445 882
Size: 617 599 232 2115 2219
Fragments arranged by size:
2219 2115 617 599 232
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2674 2704 1178
Size: 30 165 30 1271 30 4256
Fragments arranged by size:
4256 1271 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3019 1186
Size: 1833 3949
BseYI C'CCAG_C
Cuts at: 4985 5481 4985
Size: 496 5286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2643 3843 3928 4999 2016
Size: 627 1200 85 1071 2799
Fragments arranged by size:
2799 1200 1071 627 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2023 2643 2918 3101 3843 3928
Size: 399 226 7 620 275 183 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 620 399 275 226 183
85 7
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3137 844
Size: 2293 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2111 3669 1394
Size: 429 288 1558 3507
Fragments arranged by size:
3507 1558 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 2530 2860 96
Size: 2434 330 3018
Fragments arranged by size:
3018 2434 330
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2203 3112 3479 3864 3881 5333 5419 5431
Size: 231 909 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 909 385 367 346 231 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2106 2576 3298 4092 4112 2106
Size: 470 722 794 20 3776
Fragments arranged by size:
3776 794 722 470 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 3229 4118
Size: 54 1425 50 275 18 213 1186 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1186 889 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2415 3712 1353
Size: 765 297 1297 3423
Fragments arranged by size:
3423 1297 765 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2198 2414 2613 2657 2732 3845
Size: 393 467 99 216 199 44 75 1113
Cuts at: 3845 4442 5229 5497 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1113 787 597 467 393 268 216
199 99 75 44
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2861 2893 4423
Size: 32 135 32 470 32 787 32 1530
Cuts at: 4423 4455 4497 4529 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1530 787 470 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2609 3126 3372 669
Size: 988 281 671 517 246 3079
Fragments arranged by size:
3079 988 671 517 281 246
HindIII A'AGCT_T
Cuts at: 748 3015 748
Size: 2267 3515
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3062 4896 5080 1305
Size: 1757 1834 184 2007
Fragments arranged by size:
2007 1834 1757 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4099 4258 518
Size: 648 2574 359 159 2042
Fragments arranged by size:
2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2457 2985 3198 3875 5023 5268 1606
Size: 427 424 528 213 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 528 427 424 245 213
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2123 3140 4685 1132
Size: 97 894 1017 1545 2229
Fragments arranged by size:
2229 1545 1017 894 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 3134 830
Size: 2304 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2033 2033
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 2607 3124 1936
Size: 671 517 4594
Fragments arranged by size:
4594 671 517
SapI GCTCTTCn'nnn_
Cuts at: 2118 2118
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 3130 4265 4946 5137 826
Size: 219 2085 1135 681 191 1471
Fragments arranged by size:
2085 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2936 3109 1872
Size: 1064 173 4545
Fragments arranged by size:
4545 1064 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2413 3886 4787 5049 820
Size: 197 926 470 1473 901 262 1553
Fragments arranged by size:
1553 1473 926 901 470 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 2432 3706 5
Size: 47 2380 1274 2081
Fragments arranged by size:
2380 2081 1274 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2551 3221 513
Size: 552 939 547 670 3074
Fragments arranged by size:
3074 939 670 552 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2753 3809 3826 3979 4164 4609
Size: 1255 588 585 1056 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1255 1056 588 585 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2530 2860 3046
Size: 274 80 33 51 1443 553 330 186
Cuts at: 3046 4028 5740 96
Size: 982 1712 138
Fragments arranged by size:
1712 1443 982 553 330 274 186 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2674 2706 3019 3051 5742 5774 2674
Size: 32 313 32 2691 32 2682
Fragments arranged by size:
2691 2682 313 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 2231 3118 2231
Size: 887 4895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2068
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2934 3107 1870
Size: 1064 173 4545
Fragments arranged by size:
4545 1064 173
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI