Restriction map for
pFN21AE3946
(Circular) (Six-base) MAPSORT of: pFN21AE3946.seq Check: 4792 from: 1 to: 5578
pFN21AE3946 5578 bp
With 182 enzymes: SgfI *
March 28, 2012 11:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2472 2472
Size: 5578
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2356 2921 1937
Size: 419 565 4594
Fragments arranged by size:
4594 565 419
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 2783 3641 5025 5293 1632
Size: 704 447 858 1384 268 1917
Fragments arranged by size:
1917 1384 858 704 447 268
AfeI AGC'GCT
Cuts at: 2072 2072
Size: 5578
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 2441 1166
Size: 1275 4303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 3321 4893 3321
Size: 1572 4006
ApaI G_GGCC'C
Cuts at: 2751 2751
Size: 5578
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2274 2329 2903 1870
Size: 73 112 219 55 574 4545
Fragments arranged by size:
4545 574 219 112 73 55
BamHI G'GATC_C
Cuts at: 2248 2908 3275 2248
Size: 660 367 4551
Fragments arranged by size:
4551 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2534 2694 2829 2899
Size: 325 217 627 546 201 160 135 70
Cuts at: 2899 4354 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 546 325 217 201 160
135 70
BanII G_rGCy'C
Cuts at: 1391 2451 2736 2751 2897 1391
Size: 1060 285 15 146 4072
Fragments arranged by size:
4072 1060 285 146 15
BbeI G_GCGC'C
Cuts at: 2337 2337
Size: 5578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2717 2854 928
Size: 971 818 137 3652
Fragments arranged by size:
3652 971 818 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3769 3803 1903
Size: 34 50 34 1748 34 3678
Fragments arranged by size:
3678 1748 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 2262 1548
Size: 714 4864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2074 2995 2074
Size: 921 4657
Bme1580I G_kGCm'C
Cuts at: 1790 2374 2438 2537 2634 2751 2789 3639 4795
Size: 584 64 99 97 117 38 850 1156
Cuts at: 4795 1790
Size: 2573
Fragments arranged by size:
2573 1156 850 584 117 99 97 64
38
BmgBI CAC'GTC
Cuts at: 1318 2815 1318
Size: 1497 4081
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2277 3309 4267
Size: 416 53 83 399 24 860 1032 958
Cuts at: 4267 442
Size: 1753
Fragments arranged by size:
1753 1032 958 860 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2211 2813 4238 1239
Size: 956 16 602 1425 2579
Fragments arranged by size:
2579 1425 956 602 16
Bpu10I CC'TnA_GC
Cuts at: 2867 2867
Size: 5578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 3703 4568 4866 2279
Size: 1424 865 298 2991
Fragments arranged by size:
2991 1424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2271 2605 4241 882
Size: 617 772 334 1636 2219
Fragments arranged by size:
2219 1636 772 617 334
BsaAI yAC'GTr
Cuts at: 493 1204 2215 3369 493
Size: 711 1011 1154 2702
Fragments arranged by size:
2702 1154 1011 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2334 2400 3385 3767
Size: 53 83 186 936 801 66 985 382
Cuts at: 3767 4402 275
Size: 635 1451
Fragments arranged by size:
1451 985 936 801 635 382 186 83
66 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2591 1178
Size: 30 165 30 1158 30 4165
Fragments arranged by size:
4165 1158 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2569 1186
Size: 889 494 4195
Fragments arranged by size:
4195 889 494
BseYI C'CCAG_C
Cuts at: 2337 4781 5277 2337
Size: 2444 496 2638
Fragments arranged by size:
2638 2444 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5005
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2736 2873 3639 3724 4795 2736
Size: 137 766 85 1071 3519
Fragments arranged by size:
3519 1071 766 137 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 2438 2451 2537 2634 2736 2751
Size: 399 584 64 13 86 97 102 15
Cuts at: 2751 2789 2873 2897 3639 3724 4795 1391
Size: 38 84 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 399 102 97 86
85 84 64 38 24 15 13
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2472 2933 844
Size: 1628 461 3489
Fragments arranged by size:
3489 1628 461
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3465 1394
Size: 429 272 1370 3507
Fragments arranged by size:
3507 1370 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 2506 4073 4247 65
Size: 1183 1258 1567 174 1396
Fragments arranged by size:
1567 1396 1258 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4222 4409 1700
Size: 110 2412 187 2869
Fragments arranged by size:
2869 2412 187 110
BsrGI T'GTAC_A
Cuts at: 96 2769 96
Size: 2673 2905
BssHII G'CGCG_C
Cuts at: 1925 2104 2106 1925
Size: 179 2 5397
Fragments arranged by size:
5397 179 2
BssSI C'ACGA_G
Cuts at: 2423 3638 4650 2423
Size: 1215 1012 3351
Fragments arranged by size:
3351 1215 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5578
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2215
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2248 2908 3275 3660 3677 5129 5215 5227
Size: 276 660 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 660 385 367 346 276 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5026
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3888 3908 3094
Size: 794 20 4764
Fragments arranged by size:
4764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DraIII CAC_nnn'GTG
Cuts at: 2496 2701 2496
Size: 205 5373
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3025 3914 5543
Size: 54 1425 50 275 18 1195 889 1629
Cuts at: 5543 8
Size: 43
Fragments arranged by size:
1629 1425 1195 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3508 1353
Size: 2155 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4165 4539 4685 387
Size: 1064 138 2576 374 146 1280
Fragments arranged by size:
2576 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2734 2734
Size: 5578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2211 2336 2783 2813 3641 4238
Size: 393 563 16 125 447 30 828 597
Cuts at: 4238 5025 5293 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 597 563 447 393 268
125 30 16
EcoO109I rG'GnC_Cy
Cuts at: 2169 2748 2169
Size: 579 4999
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 5578
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2074 2337 2827 4725 1678
Size: 396 263 490 1898 2531
Fragments arranged by size:
2531 1898 490 396 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 4219 4251 4293
Size: 32 135 32 1097 32 1518 32 42
Cuts at: 4293 4325 1373
Size: 32 2626
Fragments arranged by size:
2626 1518 1097 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2357 2922 3168 669
Size: 988 281 419 565 246 3079
Fragments arranged by size:
3079 988 565 419 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KasI G'GCGC_C
Cuts at: 2333 2333
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2523 4692 4876 1305
Size: 1218 2169 184 2007
Fragments arranged by size:
2169 2007 1218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 2441 3536 3895 4054 518
Size: 648 1051 224 1095 359 159 2042
Fragments arranged by size:
2042 1095 1051 648 359 224 159
MspA1I CmG'CkG
Cuts at: 1606 2994 3671 4819 5064 1606
Size: 1388 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 245
NarI GG'CG_CC
Cuts at: 2334 2334
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5026
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PmlI CAC'GTG
Cuts at: 2215 2215
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2169 2169
Size: 5578
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PspOMI G'GGCC_C
Cuts at: 2747 2747
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2930 830
Size: 2100 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
SacI G_AGCT'C
Cuts at: 2736 2736
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2355 2920 1936
Size: 419 565 4594
Fragments arranged by size:
4594 565 419
SanDI GG'GwC_CC
Cuts at: 2169 2169
Size: 5578
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2926 4061 4742 4933 826
Size: 219 1881 1135 681 191 1471
Fragments arranged by size:
1881 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2335 2335
Size: 5578
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2057 2331 2905 1872
Size: 185 274 574 4545
Fragments arranged by size:
4545 574 274 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 3682 4583 4845 820
Size: 197 926 351 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 901 351 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2253 3017 513
Size: 552 1188 764 3074
Fragments arranged by size:
3074 1188 764 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 3824 5536
Size: 274 80 33 51 1443 792 1055 1712
Cuts at: 5536 96
Size: 138
Fragments arranged by size:
1712 1443 1055 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2561 2593 5538 5570 2561
Size: 32 2945 32 2569
Fragments arranged by size:
2945 2569 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2812 2468
Size: 344 5234
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2055 2329 2903 1870
Size: 185 274 574 4545
Fragments arranged by size:
4545 574 274 185
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfiI SrfI SwaI