Restriction map for
pFN21AE3943
(Circular) (Six-base) MAPSORT of: pFN21AE3943.seq Check: 3584 from: 1 to: 5827
pFN21AE3943 5827 bp
With 182 enzymes: SgfI *
March 28, 2012 11:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2373 3637 278
Size: 53 83 186 936 837 1264 2468
Fragments arranged by size:
2468 1264 936 837 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3890 5274 5542 1632
Size: 2258 1384 268 1917
Fragments arranged by size:
2258 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2929 1184
Size: 1745 4082
AlwNI CAG_nnn'CTG
Cuts at: 2097 2106 3570 5142 2097
Size: 9 1464 1572 2782
Fragments arranged by size:
2782 1572 1464 9
ApaLI G'TGCA_C
Cuts at: 2384 3884 5040 2384
Size: 1500 1156 3171
Fragments arranged by size:
3171 1500 1156
ApoI r'AATT_y
Cuts at: 1493 2234 3136 3362 3514 5623 1493
Size: 741 902 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 741 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2305 2489 2555 2921 3152 1870
Size: 73 362 184 66 366 231 4545
Fragments arranged by size:
4545 366 362 231 184 73 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2503 2536 2503
Size: 33 5794
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2641 3148 4603 618
Size: 325 217 627 854 507 1455 1842
Fragments arranged by size:
1842 1455 854 627 507 325 217
BanII G_rGCy'C
Cuts at: 1391 2496 2784 3146 1391
Size: 1105 288 362 4072
Fragments arranged by size:
4072 1105 362 288
BbeI G_GCGC'C
Cuts at: 2645 2645
Size: 5827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2922 2956 4018 4052 1903
Size: 34 985 34 1062 34 3678
Fragments arranged by size:
3678 1062 985 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2942 4438 136
Size: 107 122 71 631 605 1270 1496 1525
Fragments arranged by size:
1525 1496 1270 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2305 2388 2690 3888 5044 1790
Size: 515 83 302 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 515 302 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2870 3558 4516
Size: 416 53 83 399 24 1453 688 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1453 958 688 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2348 2396 2585 2792 2798 2984 3127
Size: 798 311 48 189 207 6 186 143
Cuts at: 3127 4487 1239
Size: 1360 2579
Fragments arranged by size:
2579 1360 798 311 207 189 186 143
48 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3952 4817 5115 3065
Size: 887 865 298 3777
Fragments arranged by size:
3777 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2067 4490 882
Size: 617 568 2423 2219
Fragments arranged by size:
2423 2219 617 568
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2370 2642 3039 3634
Size: 53 83 186 936 837 272 397 595
Cuts at: 3634 4016 4651 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 837 635 595 397 382 272
186 83 53
BsaWI w'CCGG_w
Cuts at: 4260 4658 4932 5079 5490 4260
Size: 398 274 147 411 4597
Fragments arranged by size:
4597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5602
Fragments arranged by size:
5602 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2981 1186
Size: 862 933 4032
Fragments arranged by size:
4032 933 862
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2582 1163
Size: 573 846 4408
Fragments arranged by size:
4408 846 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2946 3277 4038 5066 1540
Size: 275 186 945 331 761 1028 2301
Fragments arranged by size:
2301 1028 945 761 331 275 186
BsiHKAI G_wGCw'C
Cuts at: 2388 2496 2556 3888 3973 5044 2388
Size: 108 60 1332 85 1071 3171
Fragments arranged by size:
3171 1332 1071 108 85 60
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2305 2388 2496 2556 2690 2784 3146
Size: 399 515 83 108 60 134 94 362
Cuts at: 3146 3888 3973 5044 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 399 362 134 108
94 85 83 60
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2132 2436 3182 844
Size: 1288 304 746 3489
Fragments arranged by size:
3489 1288 746 304
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3714 1394
Size: 429 527 1364 3507
Fragments arranged by size:
3507 1364 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 2477 2584 2916 2929 2978 3048 1925
Size: 552 107 332 13 49 70 4704
Fragments arranged by size:
4704 552 332 107 70 49 13
BssSI C'ACGA_G
Cuts at: 3119 3887 4899 3119
Size: 768 1012 4047
Fragments arranged by size:
4047 1012 768
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2335 2832 513
Size: 552 939 331 497 3508
Fragments arranged by size:
3508 939 552 497 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2769 3052 3343 4137 4157 2769
Size: 283 291 794 20 4439
Fragments arranged by size:
4439 794 291 283 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DraIII CAC_nnn'GTG
Cuts at: 3031 3031
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2268 2403
Size: 54 1425 50 275 18 177 261 135
Cuts at: 2403 2943 3274 4163 5792 8
Size: 540 331 889 1629 43
Fragments arranged by size:
1629 1425 889 540 331 275 261 177
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2943 3274 1537
Size: 275 1131 331 4090
Fragments arranged by size:
4090 1131 331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2357 3757 1353
Size: 1004 1400 3423
Fragments arranged by size:
3423 1400 1004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 2188 4414 4788 4934 387
Size: 1064 138 456 143 2226 374 146 1280
Fragments arranged by size:
2226 1280 1064 456 374 146 143 138
EcoICRI GAG'CTC
Cuts at: 2494 2494
Size: 5827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2348 2396 2585 2792 2798 2984
Size: 393 405 311 48 189 207 6 186
Cuts at: 2984 3127 3890 4487 5274 5542 1239
Size: 143 763 597 787 268 1524
Fragments arranged by size:
1524 787 763 597 405 393 311 268
207 189 186 143 48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2996 2996
Size: 5827
EcoO109I rG'GnC_Cy
Cuts at: 2103 2540 2103
Size: 437 5390
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FspI TGC'GCA
Cuts at: 3068 4333 3068
Size: 1265 4562
HaeII r_GCGC'y
Cuts at: 1678 2133 2437 2645 2657 4974 1678
Size: 455 304 208 12 2317 2531
Fragments arranged by size:
2531 2317 455 304 208 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373
Size: 32 135 32 2896 32 42 32 2626
Fragments arranged by size:
2896 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2641 2641
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3785 4144 4303 518
Size: 648 2619 359 159 2042
Fragments arranged by size:
2619 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2337 2484 2713 2795 3243 3920 5068
Size: 511 220 147 229 82 448 677 1148
Cuts at: 5068 5313 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 511 448 245 229 220
147 82
NarI GG'CG_CC
Cuts at: 2642 2642
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4336
Fragments arranged by size:
4336 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2182 3185 4730 1132
Size: 97 953 1003 1545 2229
Fragments arranged by size:
2229 1545 1003 953 97
PasI CC'CwG_GG
Cuts at: 1348 2753 1348
Size: 1405 4422
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5827
PfoI T'CCnGG_A
Cuts at: 2373 2373
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2540 2540
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 2810 1613
Size: 1197 4630
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspXI vC'TCGA_Gb
Cuts at: 2555 2921 2555
Size: 366 5461
PstI C_TGCA'G
Cuts at: 830 2102 2129 2472 2820 3179 830
Size: 1272 27 343 348 359 3478
Fragments arranged by size:
3478 1272 359 348 343 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2484 2484
Size: 5827
SacI G_AGCT'C
Cuts at: 2496 2496
Size: 5827
SacII CC_GC'GG
Cuts at: 2338 2338
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SanDI GG'GwC_CC
Cuts at: 2540 2540
Size: 5827
SapI GCTCTTCn'nnn_
Cuts at: 2357 2357
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2098 2125 2468 2816 3175 4310 4991
Size: 219 1053 27 343 348 359 1135 681
Cuts at: 4991 5182 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1053 681 359 348 343 219
191 27
SfoI GGC'GCC
Cuts at: 2643 2643
Size: 5827
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 2307 3154 1872
Size: 435 847 4545
Fragments arranged by size:
4545 847 435
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 2921 3080 3931 4832 5094
Size: 197 926 612 366 159 851 901 262
Cuts at: 5094 820
Size: 1553
Fragments arranged by size:
1553 926 901 851 612 366 262 197
159
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 2182 3185 1132
Size: 1050 1003 3774
Fragments arranged by size:
3774 1050 1003
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 2319 2570 3112 1517
Size: 802 251 542 4232
Fragments arranged by size:
4232 802 542 251
StyI C'CwwG_G
Cuts at: 513 1065 2004 3266 513
Size: 552 939 1262 3074
Fragments arranged by size:
3074 1262 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2664 3854 3871 4024 4209 4654 325
Size: 1255 1084 1190 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1190 1084 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4073 5785 96
Size: 274 80 33 51 1443 2096 1712 138
Fragments arranged by size:
2096 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 5787 5819 2037
Size: 32 3718 32 2045
Fragments arranged by size:
3718 2045 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 2555 2921 1943
Size: 612 366 4849
Fragments arranged by size:
4849 612 366
XmaI C'CCGG_G
Cuts at: 1870 2305 3152 1870
Size: 435 847 4545
Fragments arranged by size:
4545 847 435
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2371 3635 276
Size: 53 83 186 936 837 1264 2468
Fragments arranged by size:
2468 1264 936 837 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI RsrII SexAI SfiI SrfI SwaI
XcmI