Restriction map for
pFN21AE3935
(Circular) (Six-base) MAPSORT of: pFN21AE3935.seq Check: 789 from: 1 to: 6103
pFN21AE3935 6103 bp
With 182 enzymes: SgfI *
November 18, 2016 17:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2716 2716
Size: 6103
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2169 3446 1937
Size: 232 1277 4594
Fragments arranged by size:
4594 1277 232
Acc65I G'GTAC_C
Cuts at: 2500 3424 2500
Size: 924 5179
AclI AA'CG_TT
Cuts at: 1563 2336 4230 4603 1563
Size: 773 1894 373 3063
Fragments arranged by size:
3063 1894 773 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2380 2472 2493 4166 5550 5818 1632
Size: 748 92 21 1673 1384 268 1917
Fragments arranged by size:
1917 1673 1384 748 268 92 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 2591 2627 2708 2807 3846 5418 2591
Size: 36 81 99 1039 1572 3276
Fragments arranged by size:
3276 1572 1039 99 81 36
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 2045 2372 3412 3638 3790 5899 1493
Size: 552 327 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 552 327 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 3249 3306 3428 1870
Size: 73 1306 57 122 4545
Fragments arranged by size:
4545 1306 122 73 57
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2500 3424 4879 618
Size: 325 217 627 713 924 1455 1842
Fragments arranged by size:
1842 1455 924 713 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3422 1391
Size: 2031 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2742 3251 928
Size: 971 843 509 3780
Fragments arranged by size:
3780 971 843 509
BbvCI CC'TCA_GC
Cuts at: 2895 2895
Size: 6103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2805 3995 5205 2805
Size: 1190 1210 3703
Fragments arranged by size:
3703 1210 1190
BclI T'GATC_A
Cuts at: 1548 2527 1548
Size: 979 5124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6098 6098
Size: 6103
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 4164 5320 1790
Size: 2374 1156 2573
Fragments arranged by size:
2573 2374 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2781 2817 3834
Size: 416 53 83 399 24 1364 36 1017
Cuts at: 3834 4792 442
Size: 958 1753
Fragments arranged by size:
1753 1364 1017 958 416 399 83 53
36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2715 3354 2715
Size: 639 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2685 3318 4763 1239
Size: 1446 633 1445 2579
Fragments arranged by size:
2579 1446 1445 633
Bpu10I CC'TnA_GC
Cuts at: 2649 2895 3188 2649
Size: 246 293 5564
Fragments arranged by size:
5564 293 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2761 4228 5093 5391 2761
Size: 1467 865 298 3473
Fragments arranged by size:
3473 1467 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4766 882
Size: 617 3267 2219
Fragments arranged by size:
3267 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3894 493
Size: 711 2690 2702
Fragments arranged by size:
2702 2690 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3910 4292 4927 275
Size: 53 83 186 936 2377 382 635 1451
Fragments arranged by size:
2377 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3339 3369 1178
Size: 30 165 30 1936 30 3912
Fragments arranged by size:
3912 1936 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2335 2459 2582 1186
Size: 1149 124 123 4707
Fragments arranged by size:
4707 1149 124 123
BseYI C'CCAG_C
Cuts at: 3316 5306 5802 3316
Size: 1990 496 3617
Fragments arranged by size:
3617 1990 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3377 1163
Size: 573 1641 3889
Fragments arranged by size:
3889 1641 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 3553 4314 5342 1540
Size: 275 186 1270 282 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 4164 4249 5320 4164
Size: 85 1071 4947
Fragments arranged by size:
4947 1071 85
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3422 4164 4249 5320 1391
Size: 399 1632 742 85 1071 2174
Fragments arranged by size:
2174 1632 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3049 3992 5722 3049
Size: 943 1730 3430
Fragments arranged by size:
3430 1730 943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2716 3051 3391 3458 844
Size: 1872 335 340 67 3489
Fragments arranged by size:
3489 1872 340 335 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4747 4934 1700
Size: 110 2937 187 2869
Fragments arranged by size:
2937 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6103
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 3433 3800 4185 4202 5654 5740 5752 6098
Size: 1461 367 385 17 1452 86 12 346
Cuts at: 6098 1972
Size: 1977
Fragments arranged by size:
1977 1461 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2352 2439 2493 2676 2352
Size: 87 54 183 5779
Fragments arranged by size:
5779 183 87 54
BtgI C'CryG_G
Cuts at: 513 1065 2004 2128 3343 3395 513
Size: 552 939 124 1215 52 3221
Fragments arranged by size:
3221 1215 939 552 124 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 3619 4413 4433 2066
Size: 1553 794 20 3736
Fragments arranged by size:
3736 1553 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DraIII CAC_nnn'GTG
Cuts at: 2789 2789
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3550 4439 6068
Size: 54 1425 50 275 18 1720 889 1629
Cuts at: 6068 8
Size: 43
Fragments arranged by size:
1720 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 2684 3035 3231 4033 1353
Size: 769 562 351 196 802 3423
Fragments arranged by size:
3423 802 769 562 351 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2380 2472 2493 2685 3318 4166 4763
Size: 393 748 92 21 192 633 848 597
Cuts at: 4763 5550 5818 1239
Size: 787 268 1524
Fragments arranged by size:
1524 848 787 748 633 597 393 268
192 92 21
EcoO109I rG'GnC_Cy
Cuts at: 2080 2948 3293 3302 3335 2080
Size: 868 345 9 33 4848
Fragments arranged by size:
4848 868 345 33 9
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
EcoRV GAT'ATC
Cuts at: 2392 2392
Size: 6103
FspI TGC'GCA
Cuts at: 3113 4609 3113
Size: 1496 4607
HaeII r_GCGC'y
Cuts at: 1678 5250 1678
Size: 3572 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2803 2835 3204 3236 4744
Size: 32 135 32 1231 32 369 32 1508
Cuts at: 4744 4776 4818 4850 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1508 1231 369 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 3693 669
Size: 988 281 1509 246 3079
Fragments arranged by size:
3079 1509 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 2504 3428 2504
Size: 924 5179
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4281
Fragments arranged by size:
4281 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2244 4061 4420 4579 518
Size: 648 843 235 1817 359 159 2042
Fragments arranged by size:
2042 1817 843 648 359 235 159
MspA1I CmG'CkG
Cuts at: 1606 2899 3519 4196 5344 5589 1606
Size: 1293 620 677 1148 245 2120
Fragments arranged by size:
2120 1293 1148 677 620 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2128 513
Size: 552 939 124 4488
Fragments arranged by size:
4488 939 552 124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2092 3461 5006 1132
Size: 97 863 1369 1545 2229
Fragments arranged by size:
2229 1545 1369 863 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2287 2789 2807 3371 1108
Size: 1179 502 18 564 3840
Fragments arranged by size:
3840 1179 564 502 18
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2948 3293 2948
Size: 345 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PspXI vC'TCGA_Gb
Cuts at: 3249 3306 3249
Size: 57 6046
PstI C_TGCA'G
Cuts at: 830 2713 3455 830
Size: 1883 742 3478
Fragments arranged by size:
3478 1883 742
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SanDI GG'GwC_CC
Cuts at: 2948 2948
Size: 6103
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 2709 3451 4586 5267 5458 826
Size: 219 1664 742 1135 681 191 1471
Fragments arranged by size:
1664 1471 1135 742 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3430 1872
Size: 1558 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2740 3249 3306 4207 5108 5370
Size: 197 926 797 509 57 901 901 262
Cuts at: 5370 820
Size: 1553
Fragments arranged by size:
1553 926 901 901 797 509 262 197
57
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 2932 4027 5
Size: 47 2880 1095 2081
Fragments arranged by size:
2880 2081 1095 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6103
StyI C'CwwG_G
Cuts at: 513 1065 2004 2128 3542 513
Size: 552 939 124 1414 3074
Fragments arranged by size:
3074 1414 939 552 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 3285 3308 4130 4147 4300 4485
Size: 1255 1220 485 23 822 17 153 185
Cuts at: 4485 4930 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1220 822 485 445 185 153
23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2728 4349 6061
Size: 274 80 33 51 1443 751 1621 1712
Cuts at: 6061 96
Size: 138
Fragments arranged by size:
1712 1621 1443 751 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3087 3119 3339 3360 3371 3392 6063 6095 3087
Size: 32 220 21 11 21 2671 32 3095
Fragments arranged by size:
3095 2671 220 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3032 3032
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 3249 3306 1943
Size: 1306 57 4740
Fragments arranged by size:
4740 1306 57
XmaI C'CCGG_G
Cuts at: 1870 3428 1870
Size: 1558 4545
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 6103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI