Restriction map for
pFN21AE3934
(Circular) (Six-base) MAPSORT of: pFN21AE3934.seq Check: 9175 from: 1 to: 6340
pFN21AE3934 6340 bp
With 182 enzymes: SgfI *
March 28, 2012 11:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2848 3062 3396 4403 5787 6055 1632
Size: 1216 214 334 1007 1384 268 1917
Fragments arranged by size:
1917 1384 1216 1007 334 268 214
AfeI AGC'GCT
Cuts at: 2591 2963 2591
Size: 372 5968
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 5239 6216 1203
Size: 4036 977 1327
Fragments arranged by size:
4036 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6340
AlwNI CAG_nnn'CTG
Cuts at: 2383 3248 4083 5655 2383
Size: 865 835 1572 3068
Fragments arranged by size:
3068 1572 865 835
ApaI G_GGCC'C
Cuts at: 2374 2374
Size: 6340
ApaLI G'TGCA_C
Cuts at: 2883 4397 5553 2883
Size: 1514 1156 3670
Fragments arranged by size:
3670 1514 1156
ApoI r'AATT_y
Cuts at: 1493 2201 2423 2852 3649 3875 4027 6136 1493
Size: 708 222 429 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 708 429 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2839 2941 3573 3665 1870
Size: 73 102 794 102 632 92 4545
Fragments arranged by size:
4545 794 632 102 102 92 73
BamHI G'GATC_C
Cuts at: 2782 3670 4037 2782
Size: 888 367 5085
Fragments arranged by size:
5085 888 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2687 2728 2906 3661 5116
Size: 325 217 627 900 41 178 755 1455
Cuts at: 5116 618
Size: 1842
Fragments arranged by size:
1842 1455 900 755 627 325 217 178
41
BanII G_rGCy'C
Cuts at: 1391 2374 2703 3171 3374 3659 1391
Size: 983 329 468 203 285 4072
Fragments arranged by size:
4072 983 468 329 285 203
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3140 3537 3558 928
Size: 971 1241 397 21 3710
Fragments arranged by size:
3710 1241 971 397 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4531 4565 1903
Size: 34 2594 34 3678
Fragments arranged by size:
3678 2594 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2501 4951 136
Size: 107 122 71 631 605 829 2450 1525
Fragments arranged by size:
2450 1525 829 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2374 2733 2887 2909 3509 3612 4401
Size: 228 356 359 154 22 600 103 789
Cuts at: 4401 5557 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 789 600 359 356 228 154
103 22
BmgBI CAC'GTC
Cuts at: 1318 2803 2848 1318
Size: 1485 45 4810
Fragments arranged by size:
4810 1485 45
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4071 5029 442
Size: 416 53 83 399 24 2654 958 1753
Fragments arranged by size:
2654 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3321 1056
Size: 2265 4075
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3403 3650 3403
Size: 247 6093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2426 2741 5000 1239
Size: 980 207 315 2259 2579
Fragments arranged by size:
2579 2259 980 315 207
Bpu10I CC'TnA_GC
Cuts at: 3188 3263 3284 3188
Size: 75 21 6244
Fragments arranged by size:
6244 75 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 4465 5330 5628 2324
Size: 2141 865 298 3036
Fragments arranged by size:
3036 2141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2167 2595 5003 882
Size: 617 668 428 2408 2219
Fragments arranged by size:
2408 2219 668 617 428
BsaAI yAC'GTr
Cuts at: 493 1204 3607 4131 493
Size: 711 2403 524 2702
Fragments arranged by size:
2702 2403 711 524
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2217 2247 1178
Size: 30 165 30 814 30 5271
Fragments arranged by size:
5271 814 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3455 1186
Size: 2269 4071
BseYI C'CCAG_C
Cuts at: 2373 5543 6039 2373
Size: 3170 496 2674
Fragments arranged by size:
3170 2674 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 3239 1163
Size: 573 1065 438 4264
Fragments arranged by size:
4264 1065 573 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2887 3374 4401 4486 5557 2046
Size: 841 487 1027 85 1071 2829
Fragments arranged by size:
2829 1071 1027 841 487 85
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2046 2374 2703 2733 2887 2909
Size: 399 228 28 328 329 30 154 22
Cuts at: 2909 3171 3374 3509 3612 3659 4401 4486 5557
Size: 262 203 135 103 47 742 85 1071
Cuts at: 5557 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 329 328 262 228
203 154 135 103 85 47 30 28
22
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2185 3695 844
Size: 1341 1510 3489
Fragments arranged by size:
3489 1510 1341
BsrBI CCG'CTC
Cuts at: 1394 1823 2431 4227 1394
Size: 429 608 1796 3507
Fragments arranged by size:
3507 1796 608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4835 5009 65
Size: 1183 3587 174 1396
Fragments arranged by size:
3587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3288 4984 5171 1700
Size: 110 1478 1696 187 2869
Fragments arranged by size:
2869 1696 1478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6340
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 2995 1184
Size: 1811 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 2749 2683
Size: 66 6274
BstYI r'GATC_y
Cuts at: 1972 2782 3670 4037 4422 4439 5891 5977 5989
Size: 810 888 367 385 17 1452 86 12
Cuts at: 5989 6335 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 888 810 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 3856 4650 4670 2066
Size: 1790 794 20 3736
Fragments arranged by size:
3736 1790 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2760 2795 529
Size: 35 2196 35 4074
Fragments arranged by size:
4074 2196 35 35
DraI TTT'AAA
Cuts at: 2398 3644 2398
Size: 1246 5094
DraIII CAC_nnn'GTG
Cuts at: 2061 3472 2061
Size: 1411 4929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2801 5347 809
Size: 507 1485 2546 1802
Fragments arranged by size:
2546 1802 1485 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2550 3474 3519
Size: 54 1425 50 275 18 720 924 45
Cuts at: 3519 3787 4676 6305 8
Size: 268 889 1629 43
Fragments arranged by size:
1629 1425 924 889 720 275 268 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3372 3387 4270 1353
Size: 2019 15 883 3423
Fragments arranged by size:
3423 2019 883 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3598 4927 5301 5447 387
Size: 1064 138 2009 1329 374 146 1280
Fragments arranged by size:
2009 1329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3372 3372
Size: 6340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2426 2741 2848 3062 3396 4403
Size: 393 587 207 315 107 214 334 1007
Cuts at: 4403 5000 5787 6055 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1007 787 597 587 393 334 315
268 214 207 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2708
Size: 6340
EcoO109I rG'GnC_Cy
Cuts at: 3151 3151
Size: 6340
EcoRI G'AATT_C
Cuts at: 2852 3649 4027 2852
Size: 797 378 5165
Fragments arranged by size:
5165 797 378
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 6340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2552 2368
Size: 184 6156
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2053 2593 2965 3324 5487 1678
Size: 375 540 372 359 2163 2531
Fragments arranged by size:
2531 2163 540 375 372 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 4981 5013 5055
Size: 32 135 32 608 32 2769 32 42
Cuts at: 5055 5087 1373
Size: 32 2626
Fragments arranged by size:
2769 2626 608 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3091 3684 3930 669
Size: 988 281 1153 593 246 3079
Fragments arranged by size:
3079 1153 988 593 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2688 2916 3036 5454 5638 1305
Size: 753 630 228 120 2418 184 2007
Fragments arranged by size:
2418 2007 753 630 228 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3476 10
Size: 54 1425 343 1644 2874
Fragments arranged by size:
2874 1644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2762 4298 4657 4816 518
Size: 648 948 648 1536 359 159 2042
Fragments arranged by size:
2042 1536 948 648 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2383 2404 3756 4433 5581 5826 1606
Size: 657 120 21 1352 677 1148 245 2120
Fragments arranged by size:
2120 1352 1148 677 657 245 120 21
NaeI GCC'GGC
Cuts at: 3290 3290
Size: 6340
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NgoMIV G'CCGG_C
Cuts at: 3288 3288
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 3317 1052
Size: 2265 4075
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 3698 5243 1132
Size: 97 2469 1545 2229
Fragments arranged by size:
2469 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 5239 5239
Size: 6340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2659 2964 1108
Size: 902 649 305 4484
Fragments arranged by size:
4484 902 649 305
PfoI T'CCnGG_A
Cuts at: 2235 2235
Size: 6340
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3151 3151
Size: 6340
PshAI GACnn'nnGTC
Cuts at: 1613 2898 3537 3575 1613
Size: 1285 639 38 4378
Fragments arranged by size:
4378 1285 639 38
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspOMI G'GGCC_C
Cuts at: 2370 2370
Size: 6340
PspXI vC'TCGA_Gb
Cuts at: 2045 2045
Size: 6340
PstI C_TGCA'G
Cuts at: 830 2589 3692 830
Size: 1759 1103 3478
Fragments arranged by size:
3478 1759 1103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2383 2404 2383
Size: 21 6319
SacI G_AGCT'C
Cuts at: 3374 3374
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SanDI GG'GwC_CC
Cuts at: 3151 3151
Size: 6340
SapI GCTCTTCn'nnn_
Cuts at: 3372 3372
Size: 6340
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 2318 4588 6300 2318
Size: 2270 1712 2358
Fragments arranged by size:
2358 2270 1712
SfcI C'TryA_G
Cuts at: 826 1045 2585 3688 4823 5504 5695 826
Size: 219 1540 1103 1135 681 191 1471
Fragments arranged by size:
1540 1471 1135 1103 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 2841 2943 3575 3667 1872
Size: 969 102 632 92 4545
Fragments arranged by size:
4545 969 632 102 92
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2303 4444 5345 5607 820
Size: 197 926 102 258 2141 901 262 1553
Fragments arranged by size:
2141 1553 926 901 262 258 197 102
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SrfI GCCC'GGGC
Cuts at: 2841 2841
Size: 6340
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6340
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2499 3363 3779 513
Size: 552 939 177 318 864 416 3074
Fragments arranged by size:
3074 939 864 552 416 318 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2675 3014 4367 4384 4537 4722 5167
Size: 1255 1095 339 1353 17 153 185 445
Cuts at: 5167 325
Size: 1498
Fragments arranged by size:
1498 1353 1255 1095 445 339 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 4586 6298
Size: 274 80 33 51 1443 339 2270 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
2270 1712 1443 339 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6300 6332 6300
Size: 32 6308
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 2506
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 2045 1943
Size: 102 6238
XmaI C'CCGG_G
Cuts at: 1870 2839 2941 3573 3665 1870
Size: 969 102 632 92 4545
Fragments arranged by size:
4545 969 632 102 92
XmnI GAAnn'nnTTC
Cuts at: 4469 4469
Size: 6340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I FseI FspAI KasI NarI
NruI NsiI PacI PmlI PsrI RsrII SacII SexAI
SfiI SfoI SwaI