Restriction map for
pFN21AE3931
(Circular) (Six-base) MAPSORT of: pFN21AE3931.seq Check: 518 from: 1 to: 6340
pFN21AE3931 6340 bp
With 182 enzymes: SgfI *
March 28, 2012 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3372 3372
Size: 6340
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2469 3683 1937
Size: 532 1214 4594
Fragments arranged by size:
4594 1214 532
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2675 2745 4403 5787 6055 1632
Size: 839 204 70 1658 1384 268 1917
Fragments arranged by size:
1917 1658 1384 839 268 204 70
AfeI AGC'GCT
Cuts at: 3152 3152
Size: 6340
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 5239 6216 1203
Size: 4036 977 1327
Fragments arranged by size:
4036 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3150 1184
Size: 1966 4374
AlwNI CAG_nnn'CTG
Cuts at: 3379 3454 4083 5655 3379
Size: 75 629 1572 4064
Fragments arranged by size:
4064 1572 629 75
ApaI G_GGCC'C
Cuts at: 3184 3293 3184
Size: 109 6231
ApaLI G'TGCA_C
Cuts at: 3551 4397 5553 3551
Size: 846 1156 4338
Fragments arranged by size:
4338 1156 846
ApoI r'AATT_y
Cuts at: 1493 3649 3875 4027 6136 1493
Size: 2156 226 152 2109 1697
Fragments arranged by size:
2156 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 3665 1870
Size: 73 1722 4545
Fragments arranged by size:
4545 1722 73
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2530 3407 3513 3617 3661
Size: 325 217 627 743 877 106 104 44
Cuts at: 3661 5116 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 877 743 627 325 217 106
104 44
BanII G_rGCy'C
Cuts at: 1391 3184 3293 3440 3464 3659 1391
Size: 1793 109 147 24 195 4072
Fragments arranged by size:
4072 1793 195 147 109 24
BbeI G_GCGC'C
Cuts at: 3517 3517
Size: 6340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 2148 928
Size: 971 123 126 5120
Fragments arranged by size:
5120 971 126 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4531 4565 1903
Size: 34 50 34 2510 34 3678
Fragments arranged by size:
3678 2510 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BfrBI ATG'CAT
Cuts at: 2764 2764
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4951 136
Size: 107 122 71 631 605 3279 1525
Fragments arranged by size:
3279 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 3458 3757 3458
Size: 299 6041
Bme1580I G_kGCm'C
Cuts at: 1790 2580 3184 3293 3410 3555 3620 4401 5557
Size: 790 604 109 117 145 65 781 1156
Cuts at: 5557 1790
Size: 2573
Fragments arranged by size:
2573 1156 790 781 604 145 117 109
65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3185 3220 3459
Size: 416 53 83 399 24 1768 35 239
Cuts at: 3459 4071 5029 442
Size: 612 958 1753
Fragments arranged by size:
1768 1753 958 612 416 399 239 83
53 35 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2337 2337
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2256 2624 2870 3368 5000 1239
Size: 1001 16 368 246 498 1632 2579
Fragments arranged by size:
2579 1632 1001 498 368 246 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5330 5628 4465
Size: 865 298 5177
Fragments arranged by size:
5177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 5003 882
Size: 617 1380 2124 2219
Fragments arranged by size:
2219 2124 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 3550 4131 493
Size: 711 1377 969 581 2702
Fragments arranged by size:
2702 1377 969 711 581
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2749 3514 4147 4529
Size: 53 83 186 936 1216 765 633 382
Cuts at: 4529 5164 275
Size: 635 1451
Fragments arranged by size:
1451 1216 936 765 635 633 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6115
Fragments arranged by size:
6115 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2063 2138 2141 2150 2186 2189
Size: 865 3 9 75 3 9 36 3
Cuts at: 2189 2213 2222 2237 2555 2648 3319 1186
Size: 24 9 15 318 93 671 4207
Fragments arranged by size:
4207 865 671 318 93 75 36 24
15 9 9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2097 2772 2776 2823 3240 3369 3509 5543 6039
Size: 675 4 47 417 129 140 2034 496
Cuts at: 6039 2097
Size: 2398
Fragments arranged by size:
2398 2034 675 496 417 140 129 47
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 2633 1163
Size: 573 834 63 4870
Fragments arranged by size:
4870 834 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3162 3202 3790 4551 5579 1540
Size: 275 186 1161 40 588 761 1028 2301
Fragments arranged by size:
2301 1161 1028 761 588 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 3440 3555 3609 4401 4486 5557 3440
Size: 115 54 792 85 1071 4223
Fragments arranged by size:
4223 1071 792 115 85 54
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
BsmBI CGTCTCn'nnnn_
Cuts at: 2756 2756
Size: 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2580 3184 3293 3410 3440 3464 3555
Size: 399 790 604 109 117 30 24 91
Cuts at: 3555 3609 3620 3659 4401 4486 5557 1391
Size: 54 11 39 742 85 1071 2174
Fragments arranged by size:
2174 1071 790 742 604 399 117 109
91 85 54 39 30 24 11
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2352 3372 3695 844
Size: 1235 273 1020 323 3489
Fragments arranged by size:
3489 1235 1020 323 273
BsrBI CCG'CTC
Cuts at: 1394 1823 2857 3121 3195 4227 1394
Size: 429 1034 264 74 1032 3507
Fragments arranged by size:
3507 1034 1032 429 264 74
BsrDI GCAATG_nn'
Cuts at: 65 1248 4835 5009 65
Size: 1183 3587 174 1396
Fragments arranged by size:
3587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3201 3354 4984 5171 1700
Size: 110 1391 153 1630 187 2869
Fragments arranged by size:
2869 1630 1391 187 153 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3379 794
Size: 2585 3755
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6340
BstXI CCAn_nnnn'nTGG
Cuts at: 2488 2946 2488
Size: 458 5882
BstYI r'GATC_y
Cuts at: 1972 2239 3670 4037 4422 4439 5891 5977 5989
Size: 267 1431 367 385 17 1452 86 12
Cuts at: 5989 6335 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1431 385 367 346 267 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2183 2183
Size: 6340
BtgI C'CryG_G
Cuts at: 513 1065 2793 3306 513
Size: 552 1728 513 3547
Fragments arranged by size:
3547 1728 552 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3329 487
Size: 683 56 2103 3498
Fragments arranged by size:
3498 2103 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2616 3856 4650 4670 2258
Size: 358 1240 794 20 3928
Fragments arranged by size:
3928 1240 794 358 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6305
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DraIII CAC_nnn'GTG
Cuts at: 2527 2527
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 3159 3199
Size: 54 1425 50 275 18 522 807 40
Cuts at: 3199 3787 4676 6305 8
Size: 588 889 1629 43
Fragments arranged by size:
1629 1425 889 807 588 522 275 54
50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3159 3199 3787 1537
Size: 275 1347 40 588 4090
Fragments arranged by size:
4090 1347 588 275 40
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2229 2462 2469 2651 2721 2913 4270
Size: 699 177 233 7 182 70 192 1357
Cuts at: 4270 1353
Size: 3423
Fragments arranged by size:
3423 1357 699 233 192 182 177 70
7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2324 2341 3098 4927 5301 5447
Size: 1064 138 735 17 757 1829 374 146
Cuts at: 5447 387
Size: 1280
Fragments arranged by size:
1829 1280 1064 757 735 374 146 138
17
EcoICRI GAG'CTC
Cuts at: 3438 3438
Size: 6340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2256 2471 2624 2675 2745 2870
Size: 393 608 16 215 153 51 70 125
Cuts at: 2870 3368 4403 5000 5787 6055 1239
Size: 498 1035 597 787 268 1524
Fragments arranged by size:
1524 1035 787 608 597 498 393 268
215 153 125 70 51 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2397 2397
Size: 6340
EcoO109I rG'GnC_Cy
Cuts at: 2179 3180 3290 2179
Size: 1001 110 5229
Fragments arranged by size:
5229 1001 110
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 3154 3484 3517 5487 1678
Size: 1476 330 33 1970 2531
Fragments arranged by size:
2531 1970 1476 330 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2933 2965 3010 3042 3311
Size: 32 135 32 1361 32 45 32 269
Cuts at: 3311 3343 4981 5013 5055 5087 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 1361 269 135 45 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KasI G'GCGC_C
Cuts at: 3513 3513
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 10
Size: 54 1425 343 522 3996
Fragments arranged by size:
3996 1425 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2976 4298 4657 4816 518
Size: 648 1320 490 1322 359 159 2042
Fragments arranged by size:
2042 1322 1320 648 490 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2350 2684 2776 2795 2929 3315 3388
Size: 491 253 334 92 19 134 386 73
Cuts at: 3388 3756 4433 5581 5826 1606
Size: 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 491 386 368 334 253
245 134 92 73 19
NaeI GCC'GGC
Cuts at: 3203 3356 3203
Size: 153 6187
NarI GG'CG_CC
Cuts at: 3514 3514
Size: 6340
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5788
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NgoMIV G'CCGG_C
Cuts at: 3201 3354 3201
Size: 153 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NsiI A_TGCA'T
Cuts at: 2766 2766
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2298 3698 5243 1132
Size: 97 1069 1400 1545 2229
Fragments arranged by size:
2229 1545 1400 1069 97
PasI CC'CwG_GG
Cuts at: 1348 3184 3293 1348
Size: 1836 109 4395
Fragments arranged by size:
4395 1836 109
PciI A'CATG_T
Cuts at: 5239 5239
Size: 6340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2393 1108
Size: 1285 5055
PfoI T'CCnGG_A
Cuts at: 2504 2886 2504
Size: 382 5958
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 2581 3550 2581
Size: 969 5371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2051 2083 2736 2768 4409 4441 5922 5954 2051
Size: 32 653 32 1641 32 1481 32 2437
Fragments arranged by size:
2437 1641 1481 653 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2179
Size: 6340
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspOMI G'GGCC_C
Cuts at: 3180 3289 3180
Size: 109 6231
PstI C_TGCA'G
Cuts at: 830 2349 2574 2934 3692 830
Size: 1519 225 360 758 3478
Fragments arranged by size:
3478 1519 758 360 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2097 2350 2776 2929 2097
Size: 253 426 153 5508
Fragments arranged by size:
5508 426 253 153
SacI G_AGCT'C
Cuts at: 3440 3440
Size: 6340
SacII CC_GC'GG
Cuts at: 2796 2796
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SapI GCTCTTCn'nnn_
Cuts at: 2462 2651 2721 2462
Size: 189 70 6081
Fragments arranged by size:
6081 189 70
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2345 2570 2930 3688 4823 5504 5695
Size: 219 1300 225 360 758 1135 681 191
Cuts at: 5695 826
Size: 1471
Fragments arranged by size:
1471 1300 1135 758 681 360 225 219
191
SfoI GGC'GCC
Cuts at: 3515 3515
Size: 6340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4444 5345 5607 820
Size: 197 926 2501 901 262 1553
Fragments arranged by size:
2501 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6340
StyI C'CwwG_G
Cuts at: 513 1065 2628 3476 3612 3779 513
Size: 552 1563 848 136 167 3074
Fragments arranged by size:
3074 1563 848 552 167 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2400 2429 3239 4367 4384 4537 4722
Size: 1255 820 29 810 1128 17 153 185
Cuts at: 4722 5167 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1128 820 810 445 185 153
29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 4586 6298
Size: 274 80 33 51 1443 579 2030 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
2030 1712 1443 579 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 3383 3415 6300 6332 2102
Size: 32 1249 32 2885 32 2110
Fragments arranged by size:
2885 2110 1249 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2249 1316
Size: 933 5407
XbaI T'CTAG_A
Cuts at: 2242 3676 2242
Size: 1434 4906
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2347
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 2456 4469 2456
Size: 2013 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I
EcoRV FalI FseI FspAI NruI PacI PspXI PsrI
RsrII SanDI SexAI SfiI SrfI SwaI