Restriction map for
pFN21AE3927
(Circular) (Six-base) MAPSORT of: pFN21AE3927.seq Check: 9807 from: 1 to: 6010
pFN21AE3927 6010 bp
With 182 enzymes: SgfI *
March 28, 2012 11:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 2150 4137 4510 1563
Size: 587 1987 373 3063
Fragments arranged by size:
3063 1987 587 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 3158 4073 5457 5725 1632
Size: 612 914 915 1384 268 1917
Fragments arranged by size:
1917 1384 915 914 612 268
AflII C'TTAA_G
Cuts at: 820 1017 2990 820
Size: 197 1973 3840
Fragments arranged by size:
3840 1973 197
AflIII A'CryG_T
Cuts at: 1203 2176 4909 5886 1203
Size: 973 2733 977 1327
Fragments arranged by size:
2733 1327 977 973
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6010
AlwNI CAG_nnn'CTG
Cuts at: 3753 5325 3753
Size: 1572 4438
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2229 3319 3545 3697 5806 1493
Size: 736 1090 226 152 2109 1697
Fragments arranged by size:
2109 1697 1090 736 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 3335 1870
Size: 73 1392 4545
Fragments arranged by size:
4545 1392 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3331 4786 618
Size: 325 217 627 1544 1455 1842
Fragments arranged by size:
1842 1544 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2679 3329 1391
Size: 781 507 650 4072
Fragments arranged by size:
4072 781 650 507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4201 4235 1903
Size: 34 50 34 2180 34 3678
Fragments arranged by size:
3678 2180 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 2584 1548
Size: 1036 4974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4621 136
Size: 107 122 71 631 605 2949 1525
Fragments arranged by size:
2949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 4071 5227 1790
Size: 2281 1156 2573
Fragments arranged by size:
2573 2281 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2827 3741 4699
Size: 416 53 83 399 24 1410 914 958
Cuts at: 4699 442
Size: 1753
Fragments arranged by size:
1753 1410 958 914 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 4670 1239
Size: 1338 2093 2579
Fragments arranged by size:
2579 2093 1338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2028 4135 5000 5298 2028
Size: 2107 865 298 2740
Fragments arranged by size:
2740 2107 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3817 4199 4834 275
Size: 53 83 186 936 2284 382 635 1451
Fragments arranged by size:
2284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6010
BseYI C'CCAG_C
Cuts at: 5213 5709 5213
Size: 496 5514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5437
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2679 4071 4156 5227 2172
Size: 507 1392 85 1071 2955
Fragments arranged by size:
2955 1392 1071 507 85
BsmI GAATG_Cn'
Cuts at: 2204 3521 3614 2204
Size: 1317 93 4600
Fragments arranged by size:
4600 1317 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2679 3329 4071 4156 5227 1391
Size: 399 382 507 650 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 507 399 382 85
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3365 844
Size: 2521 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4505 4679 65
Size: 1183 3257 174 1396
Fragments arranged by size:
3257 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2948 794
Size: 2154 3856
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2571 2863 3340 3707 4092 4109 5561 5647
Size: 599 292 477 367 385 17 1452 86
Cuts at: 5647 5659 6005 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 599 477 385 367 346 292
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2721 513
Size: 552 1656 3802
Fragments arranged by size:
3802 1656 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 3526 4320 4340 3526
Size: 794 20 5196
Fragments arranged by size:
5196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 2235 3314 2235
Size: 1079 4931
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3193 3457 4346
Size: 54 1425 50 275 18 1363 264 889
Cuts at: 4346 5975 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1363 889 275 264 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3940 1353
Size: 2587 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2170 2677 2170
Size: 507 5503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2577 3158 4073 4670 5457 5725
Size: 393 612 333 581 915 597 787 268
Cuts at: 5725 1239
Size: 1524
Fragments arranged by size:
1524 915 787 612 597 581 393 333
268
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2429 2429
Size: 6010
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 5157 1678
Size: 3479 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2794 2826 3034 3066 3145
Size: 32 135 32 1222 32 208 32 79
Cuts at: 3145 3177 4651 4683 4725 4757 1373
Size: 32 1474 32 42 32 2626
Fragments arranged by size:
2626 1474 1222 208 135 79 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3354 3600 669
Size: 988 281 1245 171 246 3079
Fragments arranged by size:
3079 1245 988 281 246 171
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3183 3600 3183
Size: 417 5593
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3155 5124 5308 1305
Size: 1850 1969 184 2007
Fragments arranged by size:
2007 1969 1850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3195 10
Size: 54 1425 343 1363 2825
Fragments arranged by size:
2825 1425 1363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2898 3968 4327 4486 518
Size: 648 1732 1070 359 159 2042
Fragments arranged by size:
2042 1732 1070 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2339 2606 3178 3426 4103 5251 5496 1606
Size: 733 267 572 248 677 1148 245 2120
Fragments arranged by size:
2120 1148 733 677 572 267 248 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5458
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NruI TCG'CGA
Cuts at: 2037 2037
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2180 3368 4913 1132
Size: 97 951 1188 1545 2229
Fragments arranged by size:
2229 1545 1188 951 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 2176 4909 2176
Size: 2733 3277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 1108
Size: 1402 4608
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2355 2387 2355
Size: 32 5978
PstI C_TGCA'G
Cuts at: 830 3362 830
Size: 2532 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2339 2606 3178 2339
Size: 267 572 5171
Fragments arranged by size:
5171 572 267
SacI G_AGCT'C
Cuts at: 2172 2679 2172
Size: 507 5503
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 2694 4258 5970 2694
Size: 1564 1712 2734
Fragments arranged by size:
2734 1712 1564
SfcI C'TryA_G
Cuts at: 826 1045 2846 3358 4493 5174 5365 826
Size: 219 1801 512 1135 681 191 1471
Fragments arranged by size:
1801 1471 1135 681 512 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2043 2990 4114 5015 5277 820
Size: 197 926 100 947 1124 901 262 1553
Fragments arranged by size:
1553 1124 947 926 901 262 197 100
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 2711 2773 3099 3142 3934 5
Size: 47 2659 62 326 43 792 2081
Fragments arranged by size:
2659 2081 792 326 62 47 43
StuI AGG'CCT
Cuts at: 1517 2883 1517
Size: 1366 4644
StyI C'CwwG_G
Cuts at: 513 1065 3058 3449 513
Size: 552 1993 391 3074
Fragments arranged by size:
3074 1993 552 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4037 4054 4207 4392 4837 325
Size: 1255 2457 17 153 185 445 1498
Fragments arranged by size:
2457 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2692 4256 5968
Size: 274 80 33 51 1443 715 1564 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
1712 1564 1443 715 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 2374 3241 3346 2374
Size: 867 105 5038
Fragments arranged by size:
5038 867 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 2496 4139 2496
Size: 1643 4367
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI