(Circular) (Six-base) MAPSORT of: pFN21AE3925.seq Check: 2988 from: 1 to: 5374 pFN21AE3925 5374 bp With 182 enzymes: SgfI * March 28, 2012 11:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2177 2695 2177 Size: 518 4856 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 2033 2077 2376 2454 2512 3117 4689 2033 Size: 44 299 78 58 605 1572 2718 Fragments arranged by size: 2718 1572 605 299 78 58 44 ApaI G_GGCC'C Cuts at: 2210 2233 2440 2210 Size: 23 207 5144 Fragments arranged by size: 5144 207 23 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2699 1870 Size: 73 756 4545 Fragments arranged by size: 4545 756 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2695 4150 618 Size: 325 217 627 390 518 1455 1842 Fragments arranged by size: 1842 1455 627 518 390 325 217 BanII G_rGCy'C Cuts at: 1391 2098 2210 2233 2440 2693 1391 Size: 707 112 23 207 253 4072 Fragments arranged by size: 4072 707 253 207 112 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4403 BbvCI CC'TCA_GC Cuts at: 2117 2309 2117 Size: 192 5182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3985 136 Size: 107 122 71 631 605 2313 1525 Fragments arranged by size: 2313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2210 2233 2440 2570 3435 4591 1790 Size: 420 23 207 130 865 1156 2573 Fragments arranged by size: 2573 1156 865 420 207 130 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2279 2288 2408 4034 1239 Size: 941 99 9 120 1626 2579 Fragments arranged by size: 2579 1626 941 120 99 9 Bpu10I CC'TnA_GC Cuts at: 2117 2309 2324 2117 Size: 192 15 5167 Fragments arranged by size: 5167 192 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2565 3165 493 Size: 711 1361 600 2702 Fragments arranged by size: 2702 1361 711 600 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 3181 3563 4198 Size: 53 83 186 936 654 994 382 635 Cuts at: 4198 275 Size: 1451 Fragments arranged by size: 1451 994 936 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5374 BseYI C'CCAG_C Cuts at: 2103 2433 2607 4577 5073 2103 Size: 330 174 1970 496 2404 Fragments arranged by size: 2404 1970 496 330 174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 1163 Size: 573 818 3983 Fragments arranged by size: 3983 818 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2295 2394 3435 3520 4591 2295 Size: 99 1041 85 1071 3078 Fragments arranged by size: 3078 1071 1041 99 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2210 2233 2295 2394 2440 2570 Size: 399 308 112 23 62 99 46 130 Cuts at: 2570 2693 3435 3520 4591 1391 Size: 123 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 130 123 112 99 85 62 46 23 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 2033 794 Size: 1239 4135 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2173 2173 Size: 5374 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4822 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2128 2407 2890 3684 3704 2128 Size: 279 483 794 20 3798 Fragments arranged by size: 3798 794 483 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2367 2456 Size: 54 1425 50 275 18 220 317 89 Cuts at: 2456 2559 2583 2821 3710 5339 8 Size: 103 24 238 889 1629 43 Fragments arranged by size: 1629 1425 889 317 275 238 220 103 89 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2140 3304 1353 Size: 787 1164 3423 Fragments arranged by size: 3423 1164 787 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2279 2288 2408 3437 4034 4821 Size: 393 548 99 9 120 1029 597 787 Cuts at: 4821 5089 1239 Size: 268 1524 Fragments arranged by size: 1524 1029 787 597 548 393 268 120 99 9 EcoNI CCTnn'n_nnAGG Cuts at: 2458 2458 Size: 5374 EcoO109I rG'GnC_Cy Cuts at: 2074 2206 2207 2230 2074 Size: 132 1 23 5218 Fragments arranged by size: 5218 132 23 1 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2402 4521 1678 Size: 724 2119 2531 Fragments arranged by size: 2531 2119 724 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373 Size: 32 135 32 2443 32 42 32 2626 Fragments arranged by size: 2626 2443 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2181 2699 2181 Size: 518 4856 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 2532 4488 4672 1305 Size: 918 309 1956 184 2007 Fragments arranged by size: 2007 1956 918 309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 2458 2561 10 Size: 54 1425 343 537 89 103 2823 Fragments arranged by size: 2823 1425 537 343 103 89 54 MslI CAynn'nnrTG Cuts at: 518 1166 2579 3332 3691 3850 518 Size: 648 1413 753 359 159 2042 Fragments arranged by size: 2042 1413 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2308 2317 2487 2611 2790 3467 4615 Size: 501 201 9 170 124 179 677 1148 Cuts at: 4615 4860 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 501 245 201 179 170 124 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 2077 1348 Size: 729 4645 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 2077 2454 1108 Size: 969 377 4028 Fragments arranged by size: 4028 969 377 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PmlI CAC'GTG Cuts at: 2565 2565 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2190 1613 Size: 577 4797 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PspOMI G'GGCC_C Cuts at: 2206 2229 2436 2206 Size: 23 207 5144 Fragments arranged by size: 5144 207 23 PstI C_TGCA'G Cuts at: 830 2029 2038 2238 2261 2414 2469 2492 2726 Size: 1199 9 200 23 153 55 23 234 Cuts at: 2726 830 Size: 3478 Fragments arranged by size: 3478 1199 234 200 153 55 23 23 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 PvuII CAG'CTG Cuts at: 2107 2308 2317 2487 2611 2107 Size: 201 9 170 124 4870 Fragments arranged by size: 4870 201 170 124 9 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SapI GCTCTTCn'nnn_ Cuts at: 2140 2140 Size: 5374 SbfI CC_TGCA'GG Cuts at: 2029 2238 2726 2029 Size: 209 488 4677 Fragments arranged by size: 4677 488 209 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2025 2034 2234 2257 2410 2465 2488 Size: 219 980 9 200 23 153 55 23 Cuts at: 2488 2722 3857 4538 4729 826 Size: 234 1135 681 191 1471 Fragments arranged by size: 1471 1135 980 681 234 219 200 191 153 55 23 23 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 2171 1517 Size: 654 4720 StyI C'CwwG_G Cuts at: 513 1065 2181 2286 2459 2813 513 Size: 552 1116 105 173 354 3074 Fragments arranged by size: 3074 1116 552 354 173 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2007 3401 3418 3571 3756 4201 325 Size: 1255 427 1394 17 153 185 445 1498 Fragments arranged by size: 1498 1394 1255 445 427 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3620 5332 Size: 274 80 33 51 1443 30 1613 1712 Cuts at: 5332 96 Size: 138 Fragments arranged by size: 1712 1613 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5334 5366 5334 Size: 32 5342 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI