Restriction map for
pFN21AE3924
(Circular) (Six-base) MAPSORT of: pFN21AE3924.seq Check: 3597 from: 1 to: 5743
pFN21AE3924 5743 bp
With 182 enzymes: SgfI *
March 28, 2012 11:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3806 5190 5458 1632
Size: 2174 1384 268 1917
Fragments arranged by size:
2174 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 2257 2536 4642 5619 1203
Size: 1054 279 2106 977 1327
Fragments arranged by size:
2106 1327 1054 977 279
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2209 2353 2611 3486 5058 2209
Size: 144 258 875 1572 2894
Fragments arranged by size:
2894 1572 875 258 144
ApaI G_GGCC'C
Cuts at: 2704 2704
Size: 5743
ApaLI G'TGCA_C
Cuts at: 2881 3800 4956 2881
Size: 919 1156 3668
Fragments arranged by size:
3668 1156 919
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 3068 1870
Size: 73 1125 4545
Fragments arranged by size:
4545 1125 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2647 2907 3064 4519
Size: 325 217 627 309 551 260 157 1455
Cuts at: 4519 618
Size: 1842
Fragments arranged by size:
1842 1455 627 551 325 309 260 217
157
BanII G_rGCy'C
Cuts at: 1391 2704 3062 1391
Size: 1313 358 4072
Fragments arranged by size:
4072 1313 358
BbeI G_GCGC'C
Cuts at: 2100 2651 2100
Size: 551 5192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2488 2557 928
Size: 971 589 69 4114
Fragments arranged by size:
4114 971 589 69
BbvCI CC'TCA_GC
Cuts at: 2612 2612
Size: 5743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3635 4845 2247
Size: 1388 1210 3145
Fragments arranged by size:
3145 1388 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2407 4354 136
Size: 107 122 71 631 605 735 1947 1525
Fragments arranged by size:
1947 1525 735 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2265 2704 2885 3804 4960 1790
Size: 475 439 181 919 1156 2573
Fragments arranged by size:
2573 1156 919 475 439 181
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2363 2510 2915 4403 1239
Size: 914 210 147 405 1488 2579
Fragments arranged by size:
2579 1488 914 405 210 147
Bpu10I CC'TnA_GC
Cuts at: 2419 2612 2419
Size: 193 5550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3868 4733 5031 3868
Size: 865 298 4580
Fragments arranged by size:
4580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2601 3534 493
Size: 711 1397 933 2702
Fragments arranged by size:
2702 1397 933 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2648 3550 3932
Size: 53 83 186 936 564 551 902 382
Cuts at: 3932 4567 275
Size: 635 1451
Fragments arranged by size:
1451 936 902 635 564 551 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2257 2488 2867 2912 1186
Size: 1045 26 231 379 45 4017
Fragments arranged by size:
4017 1045 379 231 45 26
BseYI C'CCAG_C
Cuts at: 2722 2943 4946 5442 2722
Size: 221 2003 496 3023
Fragments arranged by size:
3023 2003 496 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2726 2801 2986 1163
Size: 573 351 639 75 185 3920
Fragments arranged by size:
3920 639 573 351 185 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3193 3954 4982 1540
Size: 275 186 15 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2885 3804 3889 4960 2885
Size: 919 85 1071 3668
Fragments arranged by size:
3668 1071 919 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2704 2885 3062 3804 3889 4960
Size: 399 475 439 181 177 742 85 1071
Cuts at: 4960 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 475 439 399 181 177
85
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 3098 844
Size: 1481 773 3489
Fragments arranged by size:
3489 1481 773
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 2749 2889 794
Size: 1559 396 140 3648
Fragments arranged by size:
3648 1559 396 140
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2620 3073 3440 3825 3842 5294 5380 5392
Size: 648 453 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 648 453 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2627 2696 2627
Size: 69 5674
BtgI C'CryG_G
Cuts at: 513 1065 2004 2068 2278 2557 2757 2821 513
Size: 552 939 64 210 279 200 64 3435
Fragments arranged by size:
3435 939 552 279 210 200 64 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2232 487
Size: 683 56 1006 3998
Fragments arranged by size:
3998 1006 683 56
BtsI GCAGTG_nn'
Cuts at: 2735 3259 4053 4073 2735
Size: 524 794 20 4405
Fragments arranged by size:
4405 794 524 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DraIII CAC_nnn'GTG
Cuts at: 2749 2889 2961 2749
Size: 140 72 5531
Fragments arranged by size:
5531 140 72
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2451 2577
Size: 54 1425 50 275 18 183 438 126
Cuts at: 2577 2929 3190 4079 5708 8
Size: 352 261 889 1629 43
Fragments arranged by size:
1629 1425 889 438 352 275 261 183
126 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3190 1537
Size: 275 201 1177 4090
Fragments arranged by size:
4090 1177 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 3673 1353
Size: 2320 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2993 4330 4704 4850 387
Size: 1064 138 1404 1337 374 146 1280
Fragments arranged by size:
1404 1337 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2363 2510 2915 3806 4403 5190
Size: 393 521 210 147 405 891 597 787
Cuts at: 5190 5458 1239
Size: 268 1524
Fragments arranged by size:
1524 891 787 597 521 405 393 268
210 147
EcoNI CCTnn'n_nnAGG
Cuts at: 2213 2345 2507 2213
Size: 132 162 5449
Fragments arranged by size:
5449 162 132
EcoO109I rG'GnC_Cy
Cuts at: 2329 2512 2632 2692 2700 2841 2329
Size: 183 120 60 8 141 5231
Fragments arranged by size:
5231 183 141 120 60 8
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2100 2434 2483 2612 2651 4890 1678
Size: 422 334 49 129 39 2239 2531
Fragments arranged by size:
2531 2239 422 334 129 49 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2499 2531 2605 2637 4384
Size: 32 135 32 927 32 74 32 1747
Cuts at: 4384 4416 4458 4490 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1747 927 135 74 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2096 2647 2096
Size: 551 5192
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 10
Size: 54 1425 343 747 3174
Fragments arranged by size:
3174 1425 747 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3701 4060 4219 518
Size: 648 1071 1464 359 159 2042
Fragments arranged by size:
2042 1464 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2530 2770 2788 2823 3159 3836 4984
Size: 464 460 240 18 35 336 677 1148
Cuts at: 4984 5229 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 464 460 336 245 240
35 18
NarI GG'CG_CC
Cuts at: 2097 2648 2097
Size: 551 5192
NcoI C'CATG_G
Cuts at: 513 1065 2004 2757 513
Size: 552 939 753 3499
Fragments arranged by size:
3499 939 753 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2261 2303 2540 3101 4646 1132
Size: 97 1032 42 237 561 1545 2229
Fragments arranged by size:
2229 1545 1032 561 237 97 42
PasI CC'CwG_GG
Cuts at: 1348 2209 1348
Size: 861 4882
PciI A'CATG_T
Cuts at: 2257 2536 4642 2257
Size: 279 2106 3358
Fragments arranged by size:
3358 2106 279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 1108
Size: 1101 4642
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2692 2632
Size: 60 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2700 2700
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2358 2769 3095 830
Size: 1528 411 326 3478
Fragments arranged by size:
3478 1528 411 326
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2770 2770
Size: 5743
SacII CC_GC'GG
Cuts at: 2071 2824 2071
Size: 753 4990
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2063 3991 5703 2063
Size: 1928 1712 2103
Fragments arranged by size:
2103 1928 1712
SexAI A'CCwGG_T
Cuts at: 2745 2745
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 2354 2713 2765 3091 4226 4907 5098
Size: 219 1309 359 52 326 1135 681 191
Cuts at: 5098 826
Size: 1471
Fragments arranged by size:
1471 1309 1135 681 359 326 219 191
52
SfoI GGC'GCC
Cuts at: 2098 2649 2098
Size: 551 5192
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3847 4748 5010 820
Size: 197 926 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 2303 3101 1132
Size: 1171 798 3774
Fragments arranged by size:
3774 1171 798
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 2757 2847 3182 513
Size: 552 939 753 90 335 3074
Fragments arranged by size:
3074 939 753 552 335 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2925 3770 3787 3940 4125 4570 325
Size: 1255 1345 845 17 153 185 445 1498
Fragments arranged by size:
1498 1345 1255 845 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2061 3989 5701
Size: 274 80 33 51 1443 84 1928 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1928 1712 1443 274 138 84 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI
XcmI