Restriction map for
pFN21AE3922
(Circular) (Six-base) MAPSORT of: pFN21AE3922.seq Check: 8745 from: 1 to: 7432
pFN21AE3922 7432 bp
With 182 enzymes: SgfI *
March 28, 2012 11:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5242 278
Size: 53 83 186 936 3706 2468
Fragments arranged by size:
3706 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4775 1937
Size: 2838 4594
Acc65I G'GTAC_C
Cuts at: 4753 4753
Size: 7432
AclI AA'CG_TT
Cuts at: 1563 5559 5932 1563
Size: 3996 373 3063
Fragments arranged by size:
3996 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 5495 6879 7147 1632
Size: 1281 2582 1384 268 1917
Fragments arranged by size:
2582 1917 1384 1281 268
AfeI AGC'GCT
Cuts at: 2368 2368
Size: 7432
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7235
AflIII A'CryG_T
Cuts at: 1203 2191 2817 3384 6331 7308 1203
Size: 988 626 567 2947 977 1327
Fragments arranged by size:
2947 1327 988 977 626 567
AgeI A'CCGG_T
Cuts at: 6263 6263
Size: 7432
AhdI GACnn_n'nnGTC
Cuts at: 6161 6161
Size: 7432
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3765 4655 1184
Size: 2581 890 3961
Fragments arranged by size:
3961 2581 890
AlwNI CAG_nnn'CTG
Cuts at: 3242 5175 6747 3242
Size: 1933 1572 3927
Fragments arranged by size:
3927 1933 1572
ApaLI G'TGCA_C
Cuts at: 3638 5489 6645 3638
Size: 1851 1156 4425
Fragments arranged by size:
4425 1851 1156
ApoI r'AATT_y
Cuts at: 1493 2158 2165 3437 4072 4741 4967 5119 7228
Size: 665 7 1272 635 669 226 152 2109
Cuts at: 7228 1493
Size: 1697
Fragments arranged by size:
2109 1697 1272 669 665 635 226 152
7
AseI AT'TA_AT
Cuts at: 160 1032 2105 2747 5986 160
Size: 872 1073 642 3239 1606
Fragments arranged by size:
3239 1606 1073 872 642
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7432
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2761 4757 1870
Size: 73 132 686 1996 4545
Fragments arranged by size:
4545 1996 686 132 73
BamHI G'GATC_C
Cuts at: 4762 5129 4762
Size: 367 7065
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4165 4753 6208 618
Size: 325 217 627 2378 588 1455 1842
Fragments arranged by size:
2378 1842 1455 627 588 325 217
BanII G_rGCy'C
Cuts at: 1391 4751 1391
Size: 3360 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2808 4473 928
Size: 971 909 1665 3887
Fragments arranged by size:
3887 1665 971 909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 5623 5657 1903
Size: 34 2120 34 1532 34 3678
Fragments arranged by size:
3678 2120 1532 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5324 6534 5324
Size: 1210 6222
BclI T'GATC_A
Cuts at: 1548 2125 3910 1548
Size: 577 1785 5070
Fragments arranged by size:
5070 1785 577
BfrBI ATG'CAT
Cuts at: 2183 3553 3845 4632 2183
Size: 1370 292 787 4983
Fragments arranged by size:
4983 1370 787 292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6043 136
Size: 107 122 71 631 605 4371 1525
Fragments arranged by size:
4371 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3942 7427 3942
Size: 3485 3947
BlpI GC'TnA_GC
Cuts at: 2924 4849 2924
Size: 1925 5507
Bme1580I G_kGCm'C
Cuts at: 1790 3642 5493 6649 1790
Size: 1852 1851 1156 2573
Fragments arranged by size:
2573 1852 1851 1156
BmgBI CAC'GTC
Cuts at: 1318 2192 2992 1318
Size: 874 800 5758
Fragments arranged by size:
5758 874 800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3850 5163
Size: 416 53 83 399 24 1595 838 1313
Cuts at: 5163 6121 442
Size: 958 1753
Fragments arranged by size:
1753 1595 1313 958 838 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4511
Size: 7432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 3219 6092 1239
Size: 1616 364 2873 2579
Fragments arranged by size:
2873 2579 1616 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4461 4628 4661 5557 6422 6720 4461
Size: 167 33 896 865 298 5173
Fragments arranged by size:
5173 896 865 298 167 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6095 882
Size: 617 4596 2219
Fragments arranged by size:
4596 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5223 493
Size: 711 4019 2702
Fragments arranged by size:
4019 2702 711
BsaBI GATnn'nnATC
Cuts at: 7426 7426
Size: 7432
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5239 5621 6256 275
Size: 53 83 186 936 3706 382 635 1451
Fragments arranged by size:
3706 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5865 6263 6537 6684 7095 5865
Size: 398 274 147 411 6202
Fragments arranged by size:
6202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2858 3688 3718 1178
Size: 30 165 30 1425 30 830 30 4892
Fragments arranged by size:
4892 1425 830 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2676 2688 4496 1186
Size: 1490 12 1808 4122
Fragments arranged by size:
4122 1808 1490 12
BseYI C'CCAG_C
Cuts at: 3935 6635 7131 3935
Size: 2700 496 4236
Fragments arranged by size:
4236 2700 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 2564 3339 1163
Size: 573 741 87 775 5256
Fragments arranged by size:
5256 775 741 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4882 5643 6671 1540
Size: 275 186 2881 761 1028 2301
Fragments arranged by size:
2881 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3642 5493 5578 6649 3642
Size: 1851 85 1071 4425
Fragments arranged by size:
4425 1851 1071 85
BsmI GAATG_Cn'
Cuts at: 2183 2246 3610 4943 5036 2183
Size: 63 1364 1333 93 4579
Fragments arranged by size:
4579 1364 1333 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3642 4751 5493 5578 6649 1391
Size: 399 1852 1109 742 85 1071 2174
Fragments arranged by size:
2174 1852 1109 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7095 7095
Size: 7432
BspHI T'CATG_A
Cuts at: 5321 7051 5321
Size: 1730 5702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4787 844
Size: 3943 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5319 1394
Size: 429 3496 3507
Fragments arranged by size:
3507 3496 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 4085 4161 5927 6101 65
Size: 1183 966 1871 76 1766 174 1396
Fragments arranged by size:
1871 1766 1396 1183 966 174 76
BsrFI r'CCGG_y
Cuts at: 1700 1810 3354 6076 6263 1700
Size: 110 1544 2722 187 2869
Fragments arranged by size:
2869 2722 1544 187 110
BsrGI T'GTAC_A
Cuts at: 96 2300 2713 96
Size: 2204 413 4815
Fragments arranged by size:
4815 2204 413
BssHII G'CGCG_C
Cuts at: 1925 2057 1925
Size: 132 7300
BssSI C'ACGA_G
Cuts at: 5492 6504 5492
Size: 1012 6420
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 3728 794
Size: 1757 1177 4498
Fragments arranged by size:
4498 1757 1177
BstBI TT'CG_AA
Cuts at: 2998 4246 6236 2998
Size: 1248 1990 4194
Fragments arranged by size:
4194 1990 1248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7432
BstYI r'GATC_y
Cuts at: 1972 2646 3942 4432 4762 5129 5514 5531 6983
Size: 674 1296 490 330 367 385 17 1452
Cuts at: 6983 7069 7081 7427 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1296 674 490 385 367 346
330 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2490 3763 513
Size: 552 939 60 426 1273 4182
Fragments arranged by size:
4182 1273 939 552 426 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 487
Size: 683 56 804 5889
Fragments arranged by size:
5889 804 683 56
BtsI GCAGTG_nn'
Cuts at: 2504 2552 4948 5742 5762 2504
Size: 48 2396 794 20 4174
Fragments arranged by size:
4174 2396 794 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7397
DraI TTT'AAA
Cuts at: 3465 3577 4736 3465
Size: 112 1159 6161
Fragments arranged by size:
6161 1159 112
DraIII CAC_nnn'GTG
Cuts at: 4535 4535
Size: 7432
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6439 809
Size: 507 5123 1802
Fragments arranged by size:
5123 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4879 5768 7397
Size: 54 1425 50 275 18 3049 889 1629
Cuts at: 7397 8
Size: 43
Fragments arranged by size:
3049 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4879 1537
Size: 275 3067 4090
Fragments arranged by size:
4090 3067 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2951 4155 5362 1353
Size: 1598 1204 1207 3423
Fragments arranged by size:
3423 1598 1207 1204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2446 6019 6393 6539 387
Size: 1064 138 857 3573 374 146 1280
Fragments arranged by size:
3573 1280 1064 857 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2855 2913 3219 5495 6092 6879 7147
Size: 393 1223 58 306 2276 597 787 268
Cuts at: 7147 1239
Size: 1524
Fragments arranged by size:
2276 1524 1223 787 597 393 306 268
58
EcoO109I rG'GnC_Cy
Cuts at: 3239 4525 3239
Size: 1286 6146
EcoRI G'AATT_C
Cuts at: 4741 5119 4741
Size: 378 7054
EcoRV GAT'ATC
Cuts at: 3664 3664
Size: 7432
FspI TGC'GCA
Cuts at: 5938 5938
Size: 7432
HaeII r_GCGC'y
Cuts at: 1678 2370 6579 1678
Size: 692 4209 2531
Fragments arranged by size:
4209 2531 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 3409 3441 3524
Size: 32 135 32 1256 32 549 32 83
Cuts at: 3524 3556 3800 3817 3832 3849 4031 4063 6073
Size: 32 244 17 15 17 182 32 2010
Cuts at: 6073 6105 6147 6179 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2010 1256 549 244 182 135 83
42 32 32 32 32 32 32 32
32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 4776 5022 669
Size: 988 281 439 2399 246 3079
Fragments arranged by size:
3079 2399 988 439 281 246
HindIII A'AGCT_T
Cuts at: 748 2203 3375 748
Size: 1455 1172 4805
Fragments arranged by size:
4805 1455 1172
HpaI GTT'AAC
Cuts at: 5022 5022
Size: 7432
KpnI G_GTAC'C
Cuts at: 4757 4757
Size: 7432
MfeI C'AATT_G
Cuts at: 5031 5031
Size: 7432
MluI A'CGCG_T
Cuts at: 7308 7308
Size: 7432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6546 6730 1305
Size: 5241 184 2007
Fragments arranged by size:
5241 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5610
Fragments arranged by size:
5610 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 4637 5390 5749 5908 518
Size: 648 1300 2171 753 359 159 2042
Fragments arranged by size:
2171 2042 1300 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3863 3973 4848 5525 6673 6918 1606
Size: 2257 110 875 677 1148 245 2120
Fragments arranged by size:
2257 2120 1148 875 677 245 110
NcoI C'CATG_G
Cuts at: 513 1065 2004 2490 3763 513
Size: 552 939 486 1273 4182
Fragments arranged by size:
4182 1273 939 552 486
NdeI CA'TA_TG
Cuts at: 387 4299 387
Size: 3912 3520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7432
NotI GC'GGCC_GC
Cuts at: 4879 4879
Size: 7432
NsiI A_TGCA'T
Cuts at: 2185 3555 3847 4634 2185
Size: 1370 292 787 4983
Fragments arranged by size:
4983 1370 787 292
NspI r_CATG'y
Cuts at: 1132 1229 2465 2513 2821 3388 4369 4632 4642
Size: 97 1236 48 308 567 981 263 10
Cuts at: 4642 4790 6335 1132
Size: 148 1545 2229
Fragments arranged by size:
2229 1545 1236 981 567 308 263 148
97 48 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7432
PciI A'CATG_T
Cuts at: 2817 3384 6331 2817
Size: 567 2947 3918
Fragments arranged by size:
3918 2947 567
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3242 4042 4535 1108
Size: 2134 800 493 4005
Fragments arranged by size:
4005 2134 800 493
PfoI T'CCnGG_A
Cuts at: 3235 3409 3235
Size: 174 7258
PmeI GTTT'AAAC
Cuts at: 4736 4736
Size: 7432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5501 5533 7014 7046 5501
Size: 32 1481 32 5887
Fragments arranged by size:
5887 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3239 4525 3239
Size: 1286 6146
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7432
PsiI TTA'TAA
Cuts at: 5002 5002
Size: 7432
PstI C_TGCA'G
Cuts at: 830 4784 830
Size: 3954 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7432
PvuII CAG'CTG
Cuts at: 3863 3973 3863
Size: 110 7322
SalI G'TCGA_C
Cuts at: 1936 4774 1936
Size: 2838 4594
SanDI GG'GwC_CC
Cuts at: 4525 4525
Size: 7432
SapI GCTCTTCn'nnn_
Cuts at: 2951 2951
Size: 7432
SbfI CC_TGCA'GG
Cuts at: 4784 4784
Size: 7432
ScaI AGT'ACT
Cuts at: 5680 7392 5680
Size: 1712 5720
SfcI C'TryA_G
Cuts at: 826 1045 4780 5915 6596 6787 826
Size: 219 3735 1135 681 191 1471
Fragments arranged by size:
3735 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7432
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7432
SmaI CCC'GGG
Cuts at: 1872 4759 1872
Size: 2887 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4476 4643 4676 5536 6437 6699
Size: 197 926 2533 167 33 860 901 262
Cuts at: 6699 820
Size: 1553
Fragments arranged by size:
2533 1553 926 901 860 262 197 167
33
SnaBI TAC'GTA
Cuts at: 493 5223 493
Size: 4730 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7432
SphI G_CATG'C
Cuts at: 1132 4369 4790 1132
Size: 3237 421 3774
Fragments arranged by size:
3774 3237 421
SspI AAT'ATT
Cuts at: 5 52 3982 5356 5
Size: 47 3930 1374 2081
Fragments arranged by size:
3930 2081 1374 47
StuI AGG'CCT
Cuts at: 1517 2397 1517
Size: 880 6552
StyI C'CwwG_G
Cuts at: 513 1065 2004 2490 3763 4871 513
Size: 552 939 486 1273 1108 3074
Fragments arranged by size:
3074 1273 1108 939 552 486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4544 5459 5476 5629 5814 6259 325
Size: 1255 2964 915 17 153 185 445 1498
Fragments arranged by size:
2964 1498 1255 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2300 2607 2713
Size: 274 80 33 51 1443 323 307 106
Cuts at: 2713 5678 7390 96
Size: 2965 1712 138
Fragments arranged by size:
2965 1712 1443 323 307 274 138 106
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3688 3720 7392 7424 3688
Size: 32 3672 32 3696
Fragments arranged by size:
3696 3672 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7432
XbaI T'CTAG_A
Cuts at: 4114 4768 4114
Size: 654 6778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3863 4443 3863
Size: 580 6852
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7432
XmaI C'CCGG_G
Cuts at: 1870 4757 1870
Size: 2887 4545
XmnI GAAnn'nnTTC
Cuts at: 3418 5561 3418
Size: 2143 5289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5240 276
Size: 53 83 186 936 3706 2468
Fragments arranged by size:
3706 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI