Restriction map for
pFN21AE3921
(Circular) (Six-base) MAPSORT of: pFN21AE3921.seq Check: 7898 from: 1 to: 5902
pFN21AE3921 5902 bp
With 182 enzymes: SgfI *
March 28, 2012 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2389 3965 5349 5617 1632
Size: 757 1576 1384 268 1917
Fragments arranged by size:
1917 1576 1384 757 268
AfeI AGC'GCT
Cuts at: 3168 3168
Size: 5902
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3034 1184
Size: 1850 4052
AlwNI CAG_nnn'CTG
Cuts at: 2621 3132 3645 5217 2621
Size: 511 513 1572 3306
Fragments arranged by size:
3306 1572 513 511
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2335 2518 2761 3002 3211 3437 3589 5698
Size: 842 183 243 241 209 226 152 2109
Cuts at: 5698 1493
Size: 1697
Fragments arranged by size:
2109 1697 842 243 241 226 209 183
152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 3227 1870
Size: 73 1284 4545
Fragments arranged by size:
4545 1284 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3221 1391
Size: 1830 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2179 2213 4093 4127 1903
Size: 34 242 34 1880 34 3678
Fragments arranged by size:
3678 1880 242 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 2560 1548
Size: 1012 4890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3139 4562 1239
Size: 1900 1423 2579
Fragments arranged by size:
2579 1900 1423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2264 4565 882
Size: 617 765 2301 2219
Fragments arranged by size:
2301 2219 765 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 3709 4091 4726
Size: 53 83 186 936 500 1676 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1676 1451 936 635 500 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3121 3151 1178
Size: 30 165 30 1718 30 3929
Fragments arranged by size:
3929 1718 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2111 2114 2123 1186
Size: 856 69 3 9 4965
Fragments arranged by size:
4965 856 69 9 3
BseYI C'CCAG_C
Cuts at: 2621 5105 5601 2621
Size: 2484 496 2922
Fragments arranged by size:
2922 2484 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2723 1163
Size: 573 987 4342
Fragments arranged by size:
4342 987 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3352 4113 5141 1540
Size: 275 186 60 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 3963 4048 5119 3963
Size: 85 1071 4746
Fragments arranged by size:
4746 1071 85
BsmI GAATG_Cn'
Cuts at: 3021 3413 3506 3021
Size: 392 93 5417
Fragments arranged by size:
5417 392 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2024 2024
Size: 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3221 3963 4048 5119 1391
Size: 399 1431 742 85 1071 2174
Fragments arranged by size:
2174 1431 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3094 3791 5521 3094
Size: 697 1730 3475
Fragments arranged by size:
3475 1730 697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2811 4397 4571 65
Size: 1183 1563 1586 174 1396
Fragments arranged by size:
1586 1563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 4546 4733 1700
Size: 110 232 2504 187 2869
Fragments arranged by size:
2869 2504 232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 3137 1184
Size: 1953 3949
BstYI r'GATC_y
Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897
Size: 1260 367 385 17 1452 86 12 346
Cuts at: 5897 1972
Size: 1977
Fragments arranged by size:
1977 1452 1260 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 2871 1554
Size: 1317 4585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 2988 3206 2988
Size: 218 5684
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 3349 4238
Size: 54 1425 50 275 18 228 1291 889
Cuts at: 4238 5867 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1291 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 3349 1537
Size: 275 246 1291 4090
Fragments arranged by size:
4090 1291 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 3832 1353
Size: 747 1732 3423
Fragments arranged by size:
3423 1732 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 2142 4489 4863 5009 387
Size: 1064 138 450 103 2347 374 146 1280
Fragments arranged by size:
2347 1280 1064 450 374 146 138 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2389 3139 3965 4562 5349 5617 1239
Size: 393 757 750 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 757 750 597 393 268
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2175 3170 5049 1678
Size: 497 995 1879 2531
Fragments arranged by size:
2531 1879 995 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2828 2860 2927
Size: 32 135 32 965 32 259 32 67
Cuts at: 2927 2959 3121 3153 4543 4575 4617 4649 1373
Size: 32 162 32 1390 32 42 32 2626
Fragments arranged by size:
2626 1390 965 259 162 135 67 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3246 3492 669
Size: 988 281 568 740 246 3079
Fragments arranged by size:
3079 988 740 568 281 246
HindIII A'AGCT_T
Cuts at: 748 2778 3037 748
Size: 2030 259 3613
Fragments arranged by size:
3613 2030 259
HpaI GTT'AAC
Cuts at: 2506 3492 2506
Size: 986 4916
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 3318 3995 5143 5388 1606
Size: 614 1098 677 1148 245 2120
Fragments arranged by size:
2120 1148 1098 677 614 245
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5350
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 1108
Size: 1134 4768
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 2304 3472 2304
Size: 1168 4734
PstI C_TGCA'G
Cuts at: 830 2016 3254 830
Size: 1186 1238 3478
Fragments arranged by size:
3478 1238 1186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2012 3250 4385 5066 5257 826
Size: 219 967 1238 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 967 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 2657 3146 152
Size: 2505 489 2908
Fragments arranged by size:
2908 2505 489
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 2679 3826 5
Size: 47 2627 1147 2081
Fragments arranged by size:
2627 2081 1147 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 2730 3341 513
Size: 552 1665 611 3074
Fragments arranged by size:
3074 1665 611 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 3101 3929 3946 4099 4284 4729
Size: 1255 698 823 828 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1498 1255 828 823 698 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2239
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI