Restriction map for
pFN21AE3919
(Circular) (Six-base) MAPSORT of: pFN21AE3919.seq Check: 6228 from: 1 to: 6826
pFN21AE3919 6826 bp
With 182 enzymes: SgfI *
March 28, 2012 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 4169 1937
Size: 708 1524 4594
Fragments arranged by size:
4594 1524 708
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 3013 3863 4889 6273 6541 1632
Size: 819 562 850 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 850 819 562 268
AfeI AGC'GCT
Cuts at: 3512 3512
Size: 6826
AflII C'TTAA_G
Cuts at: 820 1017 3908 820
Size: 197 2891 3738
Fragments arranged by size:
3738 2891 197
AflIII A'CryG_T
Cuts at: 1203 5725 6702 1203
Size: 4522 977 1327
Fragments arranged by size:
4522 1327 977
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2158 3064 3488 1184
Size: 974 906 424 4522
Fragments arranged by size:
4522 974 906 424
AlwNI CAG_nnn'CTG
Cuts at: 2733 2953 4569 6141 2733
Size: 220 1616 1572 3418
Fragments arranged by size:
3418 1616 1572 220
ApaLI G'TGCA_C
Cuts at: 3159 4883 6039 3159
Size: 1724 1156 3946
Fragments arranged by size:
3946 1724 1156
ApoI r'AATT_y
Cuts at: 1493 2683 3260 3373 3457 4135 4361 4513 6622
Size: 1190 577 113 84 678 226 152 2109
Cuts at: 6622 1493
Size: 1697
Fragments arranged by size:
2109 1697 1190 678 577 226 152 113
84
AseI AT'TA_AT
Cuts at: 160 1032 3075 5380 160
Size: 872 2043 2305 1606
Fragments arranged by size:
2305 2043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2815 4151 1870
Size: 73 872 1336 4545
Fragments arranged by size:
4545 1336 872 73
BamHI G'GATC_C
Cuts at: 3903 4156 4523 3903
Size: 253 367 6206
Fragments arranged by size:
6206 367 253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4147 5602 618
Size: 325 217 627 2360 1455 1842
Fragments arranged by size:
2360 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3193 4145 1391
Size: 1802 952 4072
Fragments arranged by size:
4072 1802 952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 2690 4119 928
Size: 971 306 485 1429 3635
Fragments arranged by size:
3635 1429 971 485 306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 1903
Size: 34 3080 34 3678
Fragments arranged by size:
3678 3080 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2229 4718 5928 2229
Size: 2489 1210 3127
Fragments arranged by size:
3127 2489 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BfrBI ATG'CAT
Cuts at: 3064 3251 3064
Size: 187 6639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5437 136
Size: 107 122 71 631 605 3765 1525
Fragments arranged by size:
3765 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2720 3610 6821 2720
Size: 890 3211 2725
Fragments arranged by size:
3211 2725 890
BlpI GC'TnA_GC
Cuts at: 4243 4243
Size: 6826
Bme1580I G_kGCm'C
Cuts at: 1790 3163 4887 6043 1790
Size: 1373 1724 1156 2573
Fragments arranged by size:
2573 1724 1373 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4557 5515 442
Size: 416 53 83 399 24 3140 958 1753
Fragments arranged by size:
3140 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5486 1239
Size: 4247 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 3890 4951 5816 6114 3553
Size: 337 1061 865 298 4265
Fragments arranged by size:
4265 1061 865 337 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5489 882
Size: 617 3990 2219
Fragments arranged by size:
3990 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4617 493
Size: 711 3413 2702
Fragments arranged by size:
3413 2702 711
BsaBI GATnn'nnATC
Cuts at: 6820 6820
Size: 6826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4633 5015 5650 275
Size: 53 83 186 936 3100 382 635 1451
Fragments arranged by size:
3100 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2011 3900 5259 5657 5931 6078 6489 2011
Size: 1889 1359 398 274 147 411 2348
Fragments arranged by size:
2348 1889 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6601
Fragments arranged by size:
6601 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6826
BseYI C'CCAG_C
Cuts at: 3693 6029 6525 3693
Size: 2336 496 3994
Fragments arranged by size:
3994 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2989 3034 3806 3907 1163
Size: 573 1253 45 772 101 4082
Fragments arranged by size:
4082 1253 772 573 101 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4276 5037 6065 1540
Size: 275 186 2275 761 1028 2301
Fragments arranged by size:
2301 2275 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3163 3193 4887 4972 6043 3163
Size: 30 1694 85 1071 3946
Fragments arranged by size:
3946 1694 1071 85 30
BsmI GAATG_Cn'
Cuts at: 2596 3641 4337 4430 2596
Size: 1045 696 93 4992
Fragments arranged by size:
4992 1045 696 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3163 3193 4145 4887 4972 6043 1391
Size: 399 1373 30 952 742 85 1071 2174
Fragments arranged by size:
2174 1373 1071 952 742 399 85 30
BspEI T'CCGG_A
Cuts at: 2011 3900 6489 2011
Size: 1889 2589 2348
Fragments arranged by size:
2589 2348 1889
BspHI T'CATG_A
Cuts at: 4715 6445 4715
Size: 1730 5096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3828 4181 844
Size: 2984 353 3489
Fragments arranged by size:
3489 2984 353
BsrBI CCG'CTC
Cuts at: 1394 1823 4713 1394
Size: 429 2890 3507
Fragments arranged by size:
3507 2890 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3036 3756 3795 5321 5495 65
Size: 1183 1788 720 39 1526 174 1396
Fragments arranged by size:
1788 1526 1396 1183 720 174 39
BsrFI r'CCGG_y
Cuts at: 1700 1810 5470 5657 1700
Size: 110 3660 187 2869
Fragments arranged by size:
3660 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6826
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6826
BstXI CCAn_nnnn'nTGG
Cuts at: 3572 3572
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2720 3216 3338 3610 3903 4156 4523 4908
Size: 748 496 122 272 293 253 367 385
Cuts at: 4908 4925 6377 6463 6475 6821 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 748 496 385 367 346 293
272 253 122 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3126 487
Size: 683 56 1900 4187
Fragments arranged by size:
4187 1900 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 4114 4342 5136 5156 2142
Size: 1972 228 794 20 3812
Fragments arranged by size:
3812 1972 794 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 2520 2667 3525 4130 2520
Size: 147 858 605 5216
Fragments arranged by size:
5216 858 605 147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5833 809
Size: 507 4517 1802
Fragments arranged by size:
4517 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3723 4273 5162
Size: 54 1425 50 275 18 1893 550 889
Cuts at: 5162 6791 8
Size: 1629 43
Fragments arranged by size:
1893 1629 1425 889 550 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4273 1537
Size: 275 2461 4090
Fragments arranged by size:
4090 2461 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4756 1353
Size: 3403 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 5413 5787 5933 387
Size: 1064 138 713 3111 374 146 1280
Fragments arranged by size:
3111 1280 1064 713 374 146 138
EcoICRI GAG'CTC
Cuts at: 3191 3191
Size: 6826
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 3013 3863 4889 5486 6273 6541
Size: 393 819 562 850 1026 597 787 268
Cuts at: 6541 1239
Size: 1524
Fragments arranged by size:
1524 1026 850 819 787 597 562 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2821 3874 2821
Size: 1053 5773
EcoRI G'AATT_C
Cuts at: 4135 4513 4135
Size: 378 6448
EcoRV GAT'ATC
Cuts at: 3835 3835
Size: 6826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2231 2456 2231
Size: 225 6601
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 3514 5973 1678
Size: 1836 2459 2531
Fragments arranged by size:
2531 2459 1836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 3785 3817 5467
Size: 32 135 32 1019 32 1162 32 1650
Cuts at: 5467 5499 5541 5573 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1650 1162 1019 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4098 4170 4416 669
Size: 988 281 2160 72 246 3079
Fragments arranged by size:
3079 2160 988 281 246 72
HindIII A'AGCT_T
Cuts at: 748 3111 748
Size: 2363 4463
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 2351 4425 2351
Size: 2074 4752
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2728 5940 6124 1305
Size: 1423 3212 184 2007
Fragments arranged by size:
3212 2007 1423 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3725 10
Size: 54 1425 343 1893 3111
Fragments arranged by size:
3111 1893 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3299 3675 4784 5143 5302 518
Size: 648 2133 376 1109 359 159 2042
Fragments arranged by size:
2133 2042 1109 648 376 359 159
MspA1I CmG'CkG
Cuts at: 1606 3048 3697 4124 4242 4919 6067 6312 1606
Size: 1442 649 427 118 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 649 427 245 118
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NsiI A_TGCA'T
Cuts at: 3066 3253 3066
Size: 187 6639
NspI r_CATG'y
Cuts at: 1132 1229 2145 3085 4184 5729 1132
Size: 97 916 940 1099 1545 2229
Fragments arranged by size:
2229 1545 1099 940 916 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6826
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6826
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 6408 6440 4895
Size: 32 1481 32 5281
Fragments arranged by size:
5281 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 3874 2821
Size: 1053 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PstI C_TGCA'G
Cuts at: 830 2398 4178 830
Size: 1568 1780 3478
Fragments arranged by size:
3478 1780 1568
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 3048 3697 3048
Size: 649 6177
SacI G_AGCT'C
Cuts at: 3193 3193
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SfcI C'TryA_G
Cuts at: 826 1045 2032 2394 2734 4174 5309 5990 6181
Size: 219 987 362 340 1440 1135 681 191
Cuts at: 6181 826
Size: 1471
Fragments arranged by size:
1471 1440 1135 987 681 362 340 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 2817 4153 1872
Size: 945 1336 4545
Fragments arranged by size:
4545 1336 945
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 3869 3908 4930 5831 6093
Size: 197 926 1589 337 39 1022 901 262
Cuts at: 6093 820
Size: 1553
Fragments arranged by size:
1589 1553 1022 926 901 337 262 197
39
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 2493 3931 4750 5
Size: 47 2441 1438 819 2081
Fragments arranged by size:
2441 2081 1438 819 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6826
StyI C'CwwG_G
Cuts at: 513 1065 3022 3565 3597 4265 513
Size: 552 1957 543 32 668 3074
Fragments arranged by size:
3074 1957 668 552 543 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3023 4853 4870 5023 5208 5653 325
Size: 1255 1443 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1443 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3179 5072 6784
Size: 274 80 33 51 1443 1202 1893 1712
Cuts at: 6784 96
Size: 138
Fragments arranged by size:
1893 1712 1443 1202 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6786 6818 6786
Size: 32 6794
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6826
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 2815 4151 1870
Size: 945 1336 4545
Fragments arranged by size:
4545 1336 945
XmnI GAAnn'nnTTC
Cuts at: 2425 2436 4955 2425
Size: 11 2519 4296
Fragments arranged by size:
4296 2519 11
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI