Restriction map for
pFN21AE3918
(Circular) (Six-base) MAPSORT of: pFN21AE3918.seq Check: 9434 from: 1 to: 6526
pFN21AE3918 6526 bp
With 182 enzymes: SgfI *
March 28, 2012 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3061 3061
Size: 6526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3869 1937
Size: 1932 4594
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3128 3266 4589 5973 6241 1632
Size: 1496 138 1323 1384 268 1917
Fragments arranged by size:
1917 1496 1384 1323 268 138
AfeI AGC'GCT
Cuts at: 3281 3281
Size: 6526
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 2775 5425 6402 1203
Size: 1572 2650 977 1327
Fragments arranged by size:
2650 1572 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 5255 5255
Size: 6526
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2429 1184
Size: 1245 5281
AlwNI CAG_nnn'CTG
Cuts at: 2344 2737 3700 4269 5841 2344
Size: 393 963 569 1572 3029
Fragments arranged by size:
3029 1572 963 569 393
ApaLI G'TGCA_C
Cuts at: 2318 4583 5739 2318
Size: 2265 1156 3105
Fragments arranged by size:
3105 2265 1156
ApoI r'AATT_y
Cuts at: 1493 3835 4061 4213 6322 1493
Size: 2342 226 152 2109 1697
Fragments arranged by size:
2342 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2644 2688 2868 3437 3752 3851 1870
Size: 73 701 44 180 569 315 99 4545
Fragments arranged by size:
4545 701 569 315 180 99 73 44
AvrII C'CTAG_G
Cuts at: 2080 2080
Size: 6526
BamHI G'GATC_C
Cuts at: 3856 4223 3856
Size: 367 6159
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2296 2648 3333 3407
Size: 325 217 627 267 242 352 685 74
Cuts at: 3407 3847 5302 618
Size: 440 1455 1842
Fragments arranged by size:
1842 1455 685 627 440 352 325 267
242 217 74
BanII G_rGCy'C
Cuts at: 1391 2043 2124 2925 2991 3129 3258 3512 3639
Size: 652 81 801 66 138 129 254 127
Cuts at: 3639 3845 1391
Size: 206 4072
Fragments arranged by size:
4072 801 652 254 206 138 129 127
81 66
BbeI G_GCGC'C
Cuts at: 3337 3411 3337
Size: 74 6452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5555
BbvCI CC'TCA_GC
Cuts at: 2099 2099
Size: 6526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4717 4751 1903
Size: 34 2780 34 3678
Fragments arranged by size:
3678 2780 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5137 136
Size: 107 122 71 631 605 3465 1525
Fragments arranged by size:
3465 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 3943 3943
Size: 6526
Bme1580I G_kGCm'C
Cuts at: 1790 2301 2322 2689 2751 2861 3209 3469 3747
Size: 511 21 367 62 110 348 260 278
Cuts at: 3747 3774 4587 5743 1790
Size: 27 813 1156 2573
Fragments arranged by size:
2573 1156 813 511 367 348 278 260
110 62 27 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2714 4257 5215
Size: 416 53 83 399 24 1297 1543 958
Cuts at: 5215 442
Size: 1753
Fragments arranged by size:
1753 1543 1297 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 3526 2944
Size: 582 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2264 2564 2759 2773 3143 3248 3446
Size: 788 237 300 195 14 370 105 198
Cuts at: 3446 3710 3749 3755 5186 1239
Size: 264 39 6 1431 2579
Fragments arranged by size:
2579 1431 788 370 300 264 237 198
195 105 39 14 6
Bpu10I CC'TnA_GC
Cuts at: 2099 2099
Size: 6526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2278 4651 5516 5814 2278
Size: 2373 865 298 2990
Fragments arranged by size:
2990 2373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3704 5189 882
Size: 617 2205 1485 2219
Fragments arranged by size:
2219 2205 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 2191 2515 4317 493
Size: 711 987 324 1802 2702
Fragments arranged by size:
2702 1802 987 711 324
BsaBI GATnn'nnATC
Cuts at: 6520 6520
Size: 6526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2561 3334 3408 4333
Size: 53 83 186 936 1028 773 74 925
Cuts at: 4333 4715 5350 275
Size: 382 635 1451
Fragments arranged by size:
1451 1028 936 925 773 635 382 186
83 74 53
BsaWI w'CCGG_w
Cuts at: 4959 5357 5631 5778 6189 4959
Size: 398 274 147 411 5296
Fragments arranged by size:
5296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2624 2654 3199 3229 3566
Size: 30 165 30 1221 30 545 30 337
Cuts at: 3566 3596 1178
Size: 30 4108
Fragments arranged by size:
4108 1221 545 337 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3416 3587 1186
Size: 2230 171 4125
Fragments arranged by size:
4125 2230 171
BseYI C'CCAG_C
Cuts at: 2266 2533 2725 2801 3649 5729 6225 2266
Size: 267 192 76 848 2080 496 2567
Fragments arranged by size:
2567 2080 848 496 267 192 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2496 3179 1163
Size: 573 760 683 4510
Fragments arranged by size:
4510 760 683 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3976 4737 5765 1540
Size: 275 186 1975 761 1028 2301
Fragments arranged by size:
2301 1975 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2133 2322 2991 3129 3258 4587 4672 5743
Size: 9 189 669 138 129 1329 85 1071
Cuts at: 5743 2124
Size: 2907
Fragments arranged by size:
2907 1329 1071 669 189 138 129 85
9
BsmI GAATG_Cn'
Cuts at: 3151 4037 4130 3151
Size: 886 93 5547
Fragments arranged by size:
5547 886 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2124 2133 2301 2322 2689 2751
Size: 399 253 81 9 168 21 367 62
Cuts at: 2751 2861 2925 2991 3129 3209 3258 3469 3512
Size: 110 64 66 138 80 49 211 43
Cuts at: 3512 3639 3747 3774 3845 4587 4672 5743 1391
Size: 127 108 27 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 253 211 168
138 127 110 108 85 81 80 71
66 64 62 49 43 27 21 9
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2593 3061 3881 844
Size: 1749 468 820 3489
Fragments arranged by size:
3489 1749 820 468
BsrBI CCG'CTC
Cuts at: 1394 1823 3757 4413 1394
Size: 429 1934 656 3507
Fragments arranged by size:
3507 1934 656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3594 5021 5195 65
Size: 1183 2346 1427 174 1396
Fragments arranged by size:
2346 1427 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2814 3327 5170 5357 1700
Size: 110 1004 513 1843 187 2869
Fragments arranged by size:
2869 1843 1004 513 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 2852 2984 4586 5598 2852
Size: 132 1602 1012 3780
Fragments arranged by size:
3780 1602 1012 132
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3076 3283 3486 794
Size: 2282 207 203 3834
Fragments arranged by size:
3834 2282 207 203
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6526
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2398 3121 2359
Size: 39 723 5764
Fragments arranged by size:
5764 723 39
BstYI r'GATC_y
Cuts at: 1972 3856 4223 4608 4625 6077 6163 6175 6521
Size: 1884 367 385 17 1452 86 12 346
Cuts at: 6521 1972
Size: 1977
Fragments arranged by size:
1977 1884 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2105 2105
Size: 6526
BtgI C'CryG_G
Cuts at: 513 1065 2358 3565 3660 3705 513
Size: 552 1293 1207 95 45 3334
Fragments arranged by size:
3334 1293 1207 552 95 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3799 487
Size: 683 56 2573 3214
Fragments arranged by size:
3214 2573 683 56
BtsI GCAGTG_nn'
Cuts at: 4042 4836 4856 4042
Size: 794 20 5712
Fragments arranged by size:
5712 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DraIII CAC_nnn'GTG
Cuts at: 2137 2137
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5533 809
Size: 507 4217 1802
Fragments arranged by size:
4217 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 3453 3758
Size: 54 1425 50 275 18 1135 488 305
Cuts at: 3758 3973 4862 6491 8
Size: 215 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 488 305 275 215
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2996 3134 3314 4456 1353
Size: 1643 138 180 1142 3423
Fragments arranged by size:
3423 1643 1142 180 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3260 3278 5113 5487 5633 387
Size: 1064 138 1671 18 1835 374 146 1280
Fragments arranged by size:
1835 1671 1280 1064 374 146 138 18
EcoICRI GAG'CTC
Cuts at: 2122 2989 3127 3256 2122
Size: 867 138 129 5392
Fragments arranged by size:
5392 867 138 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2264 2564 2759 2773 3128 3143
Size: 393 395 237 300 195 14 355 15
Cuts at: 3143 3248 3266 3446 3710 3749 3755 4589 5186
Size: 105 18 180 264 39 6 834 597
Cuts at: 5186 5973 6241 1239
Size: 787 268 1524
Fragments arranged by size:
1524 834 787 597 395 393 355 300
268 264 237 195 180 105 39 18
15 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2170 2612 3296 2084
Size: 86 442 684 5314
Fragments arranged by size:
5314 684 442 86
EcoO109I rG'GnC_Cy
Cuts at: 2109 2605 2734 3529 2109
Size: 496 129 795 5106
Fragments arranged by size:
5106 795 496 129
EcoRI G'AATT_C
Cuts at: 3835 4213 3835
Size: 378 6148
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 3283 3337 3411 5673 1678
Size: 1605 54 74 2262 2531
Fragments arranged by size:
2531 2262 1605 74 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2488 2520 2624 2656 5167
Size: 32 135 32 916 32 104 32 2511
Cuts at: 5167 5199 5241 5273 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2511 916 135 104 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3870 4116 669
Size: 988 281 1932 246 3079
Fragments arranged by size:
3079 1932 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KasI G'GCGC_C
Cuts at: 3333 3407 3333
Size: 74 6452
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2805 5640 5824 1305
Size: 1500 2835 184 2007
Fragments arranged by size:
2835 2007 1500 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4704
Fragments arranged by size:
4704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3071 4484 4843 5002 518
Size: 648 1905 1413 359 159 2042
Fragments arranged by size:
2042 1905 1413 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2281 2344 2590 2599 2884 3010 3088 3697
Size: 675 63 246 9 285 126 78 609
Cuts at: 3697 3942 4619 5767 6012 1606
Size: 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 609 285 246 245
245 126 78 63 9
NaeI GCC'GGC
Cuts at: 3329 3329
Size: 6526
NarI GG'CG_CC
Cuts at: 3334 3408 3334
Size: 74 6452
NcoI C'CATG_G
Cuts at: 513 1065 2358 3660 3705 513
Size: 552 1293 1302 45 3334
Fragments arranged by size:
3334 1302 1293 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NgoMIV G'CCGG_C
Cuts at: 3327 3327
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 2416 2557 2779 3884 5429 1132
Size: 97 1187 141 222 1105 1545 2229
Fragments arranged by size:
2229 1545 1187 1105 222 141 97
PasI CC'CwG_GG
Cuts at: 1348 2272 1348
Size: 924 5602
PciI A'CATG_T
Cuts at: 2775 5425 2775
Size: 2650 3876
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6526
PfoI T'CCnGG_A
Cuts at: 3498 3512 3498
Size: 14 6512
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 2734 3529 2605
Size: 129 795 5602
Fragments arranged by size:
5602 795 129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PstI C_TGCA'G
Cuts at: 830 2385 2523 2604 3387 3812 3878 830
Size: 1555 138 81 783 425 66 3478
Fragments arranged by size:
3478 1555 783 425 138 81 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 2281 2344 2599 2884 3010 3088 3697 2281
Size: 63 255 285 126 78 609 5110
Fragments arranged by size:
5110 609 285 255 126 78 63
SacI G_AGCT'C
Cuts at: 2124 2991 3129 3258 2124
Size: 867 138 129 5392
Fragments arranged by size:
5392 867 138 129
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SapI GCTCTTCn'nnn_
Cuts at: 2996 3134 3314 2996
Size: 138 180 6208
Fragments arranged by size:
6208 180 138
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2324 2381 2519 2575 2600 3383 3808
Size: 219 1279 57 138 56 25 783 425
Cuts at: 3808 3874 5009 5690 5881 826
Size: 66 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 783 681 425 219 191
138 66 57 56 25
SfoI GGC'GCC
Cuts at: 3335 3409 3335
Size: 74 6452
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 2870 3853 1872
Size: 998 983 4545
Fragments arranged by size:
4545 998 983
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2257 4630 5531 5793 820
Size: 197 926 314 2373 901 262 1553
Fragments arranged by size:
2373 1553 926 901 314 262 197
SnaBI TAC'GTA
Cuts at: 493 2515 4317 493
Size: 2022 1802 2702
Fragments arranged by size:
2702 2022 1802
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 2557 3884 1132
Size: 1425 1327 3774
Fragments arranged by size:
3774 1425 1327
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6526
StyI C'CwwG_G
Cuts at: 513 1065 2080 2233 2352 2358 3138 3660 3705
Size: 552 1015 153 119 6 780 522 45
Cuts at: 3705 3965 513
Size: 260 3074
Fragments arranged by size:
3074 1015 780 552 522 260 153 119
45 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2968 4553 4570 4723 4908 5353 325
Size: 1255 1388 1585 17 153 185 445 1498
Fragments arranged by size:
1585 1498 1388 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 4772 6484
Size: 274 80 33 51 1443 804 1991 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
1991 1712 1443 804 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3199 3231 3332 3364 6486 6518 3199
Size: 32 101 32 3122 32 3207
Fragments arranged by size:
3207 3122 101 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3062 1316
Size: 1746 4780
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 2868 3851 1870
Size: 998 983 4545
Fragments arranged by size:
4545 998 983
XmnI GAAnn'nnTTC
Cuts at: 4655 4655
Size: 6526
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI