Restriction map for
pFN21AE3917
(Circular) (Six-base) MAPSORT of: pFN21AE3917.seq Check: 610 from: 1 to: 5539
pFN21AE3917 5539 bp
With 182 enzymes: SgfI *
March 28, 2012 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2077 2077
Size: 5539
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 2882 1937
Size: 655 290 4594
Fragments arranged by size:
4594 655 290
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2344 2654 3602 4986 5254 1632
Size: 712 310 948 1384 268 1917
Fragments arranged by size:
1917 1384 948 712 310 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 2419 4370 2419
Size: 1951 3588
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 2811 1166
Size: 1645 3894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 2092 2367 2521 3282 4854 2092
Size: 275 154 761 1572 2777
Fragments arranged by size:
2777 1572 761 275 154
ApaI G_GGCC'C
Cuts at: 2172 2172
Size: 5539
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 2864 1870
Size: 73 628 293 4545
Fragments arranged by size:
4545 628 293 73
BamHI G'GATC_C
Cuts at: 2826 2869 3236 2826
Size: 43 367 5129
Fragments arranged by size:
5129 367 43
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2422 2792 2860 4315
Size: 325 217 627 222 413 370 68 1455
Cuts at: 4315 618
Size: 1842
Fragments arranged by size:
1842 1455 627 413 370 325 222 217
68
BanII G_rGCy'C
Cuts at: 1391 2172 2434 2858 1391
Size: 781 262 424 4072
Fragments arranged by size:
4072 781 424 262
BbeI G_GCGC'C
Cuts at: 2013 2796 2013
Size: 783 4756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 2701 928
Size: 971 369 433 3766
Fragments arranged by size:
3766 971 433 369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2562 2596 3730 3764 1903
Size: 34 625 34 1134 34 3678
Fragments arranged by size:
3678 1134 625 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2544 5534 2544
Size: 2990 2549
BlpI GC'TnA_GC
Cuts at: 2093 2956 2093
Size: 863 4676
Bme1580I G_kGCm'C
Cuts at: 1790 2172 2787 3600 4756 1790
Size: 382 615 813 1156 2573
Fragments arranged by size:
2573 1156 813 615 382
BmgBI CAC'GTC
Cuts at: 1318 2707 2767 1318
Size: 1389 60 4090
Fragments arranged by size:
4090 1389 60
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 3270 4228
Size: 416 53 83 399 24 712 1141 958
Cuts at: 4228 442
Size: 1753
Fragments arranged by size:
1753 1141 958 712 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2794 4199 1239
Size: 1555 1405 2579
Fragments arranged by size:
2579 1555 1405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2704 4202 882
Size: 617 1205 1498 2219
Fragments arranged by size:
2219 1498 1205 617
BsaAI yAC'GTr
Cuts at: 493 1204 2101 2740 3330 493
Size: 711 897 639 590 2702
Fragments arranged by size:
2702 897 711 639 590
BsaBI GATnn'nnATC
Cuts at: 2543 5533 2543
Size: 2990 2549
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2793 3346 3728
Size: 53 83 186 936 477 783 553 382
Cuts at: 3728 4363 275
Size: 635 1451
Fragments arranged by size:
1451 936 783 635 553 477 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2419 3972 4370 4644 4791 5202 2419
Size: 1553 398 274 147 411 2756
Fragments arranged by size:
2756 1553 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 1186
Size: 1099 4440
BseYI C'CCAG_C
Cuts at: 2252 2367 2394 4742 5238 2252
Size: 115 27 2348 496 2553
Fragments arranged by size:
2553 2348 496 115 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 1163
Size: 573 369 4597
Fragments arranged by size:
4597 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 2323 2989 3750 4778 1540
Size: 275 186 202 120 666 761 1028 2301
Fragments arranged by size:
2301 1028 761 666 275 202 186 120
BsiHKAI G_wGCw'C
Cuts at: 2474 3600 3685 4756 2474
Size: 1126 85 1071 3257
Fragments arranged by size:
3257 1126 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
BsmBI CGTCTCn'nnnn_
Cuts at: 2757 2757
Size: 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2434 2474 2787 2858 3600 3685
Size: 399 382 262 40 313 71 742 85
Cuts at: 3685 4756 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 313 262 85
71 40
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2077 2453 2894 844
Size: 1233 376 441 3489
Fragments arranged by size:
3489 1233 441 376
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 2419 4183 4370 1700
Size: 110 225 384 1764 187 2869
Fragments arranged by size:
2869 1764 384 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 2294 2308 2310 1925
Size: 369 14 2 5154
Fragments arranged by size:
5154 369 14 2
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 794
Size: 1727 3812
BstBI TT'CG_AA
Cuts at: 2463 4343 2463
Size: 1880 3659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2544 2826 2869 3236 3621 3638 5090 5176
Size: 572 282 43 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 572 385 367 346 282 86
43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2140 487
Size: 683 56 914 3886
Fragments arranged by size:
3886 914 683 56
BtsI GCAGTG_nn'
Cuts at: 2238 3055 3849 3869 2238
Size: 817 794 20 3908
Fragments arranged by size:
3908 817 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2097 2132 529
Size: 35 1533 35 3936
Fragments arranged by size:
3936 1533 35 35
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DraIII CAC_nnn'GTG
Cuts at: 2178 2178
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2320 2986 3875
Size: 54 1425 50 275 18 490 666 889
Cuts at: 3875 5504 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 666 490 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2320 2986 1537
Size: 275 508 666 4090
Fragments arranged by size:
4090 666 508 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 2597 4126 4500 4646 387
Size: 1064 138 465 543 1529 374 146 1280
Fragments arranged by size:
1529 1280 1064 543 465 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2344 2654 2794 3602 4199 4986 5254
Size: 393 712 310 140 808 597 787 268
Cuts at: 5254 1239
Size: 1524
Fragments arranged by size:
1524 808 787 712 597 393 310 268
140
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2013 2211 2535 2571 2796 4686 1678
Size: 335 198 324 36 225 1890 2531
Fragments arranged by size:
2531 1890 335 324 225 198 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373
Size: 32 135 32 2608 32 42 32 2626
Fragments arranged by size:
2626 2608 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2009 2792 2009
Size: 783 4756
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2811 3497 3856 4015 518
Size: 648 1645 686 359 159 2042
Fragments arranged by size:
2042 1645 686 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2281 2955 3632 4780 5025 1606
Size: 585 90 674 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 674 585 245 90
NaeI GCC'GGC
Cuts at: 2037 2037
Size: 5539
NarI GG'CG_CC
Cuts at: 2010 2793 2010
Size: 783 4756
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NgoMIV G'CCGG_C
Cuts at: 2035 2035
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2178 1108
Size: 1070 4469
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PmlI CAC'GTG
Cuts at: 2101 2101
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PspOMI G'GGCC_C
Cuts at: 2168 2168
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2190 2891 830
Size: 1360 701 3478
Fragments arranged by size:
3478 1360 701
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2191 2191
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SexAI A'CCwGG_T
Cuts at: 2493 2493
Size: 5539
SfcI C'TryA_G
Cuts at: 826 1045 2186 2887 4022 4703 4894 826
Size: 219 1141 701 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 701 681 219 191
SfoI GGC'GCC
Cuts at: 2011 2794 2011
Size: 783 4756
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 2419 1700
Size: 719 4820
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2571 3643 4544 4806 820
Size: 197 926 628 1072 901 262 1553
Fragments arranged by size:
1553 1072 926 901 628 262 197
SnaBI TAC'GTA
Cuts at: 493 2740 3330 493
Size: 2247 590 2702
Fragments arranged by size:
2702 2247 590
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2978 513
Size: 552 939 111 863 3074
Fragments arranged by size:
3074 939 863 552 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2767 3566 3583 3736 3921 4366 325
Size: 1255 1187 799 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1187 799 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2495 2527 5499 5531 2495
Size: 32 2972 32 2503
Fragments arranged by size:
2972 2503 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2663 1316
Size: 1347 4192
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 2571 1943
Size: 628 4911
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI XcmI