Restriction map for
pFN21AE3916
(Circular) (Six-base) MAPSORT of: pFN21AE3916.seq Check: 4083 from: 1 to: 6109
pFN21AE3916 6109 bp
With 182 enzymes: SgfI *
March 28, 2012 11:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2406 2568 2687 2406
Size: 162 119 5828
Fragments arranged by size:
5828 162 119
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 4172 5556 5824 1632
Size: 740 1800 1384 268 1917
Fragments arranged by size:
1917 1800 1384 740 268
AfeI AGC'GCT
Cuts at: 2438 2438
Size: 6109
AflII C'TTAA_G
Cuts at: 820 1017 3320 820
Size: 197 2303 3609
Fragments arranged by size:
3609 2303 197
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 2245 2547 1166
Size: 1079 302 4728
Fragments arranged by size:
4728 1079 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2637 1184
Size: 1453 4656
AlwNI CAG_nnn'CTG
Cuts at: 2133 2488 2560 2587 2767 2920 3169 3852 5424
Size: 355 72 27 180 153 249 683 1572
Cuts at: 5424 2133
Size: 2818
Fragments arranged by size:
2818 1572 683 355 249 180 153 72
27
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 3418 3644 3796 5905 1493
Size: 1925 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2408 3434 1870
Size: 73 465 1026 4545
Fragments arranged by size:
4545 1026 465 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2826 3430 4885 618
Size: 325 217 627 539 500 604 1455 1842
Fragments arranged by size:
1842 1455 627 604 539 500 325 217
BanII G_rGCy'C
Cuts at: 1391 2291 2478 3428 1391
Size: 900 187 950 4072
Fragments arranged by size:
4072 950 900 187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 928
Size: 971 453 4685
Fragments arranged by size:
4685 971 453
BbvCI CC'TCA_GC
Cuts at: 2219 2429 2467 2219
Size: 210 38 5861
Fragments arranged by size:
5861 210 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2600 2634 3359 3393 4300 4334 1903
Size: 34 663 34 725 34 907 34 3678
Fragments arranged by size:
3678 907 725 663 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2064 3129 6104 2064
Size: 1065 2975 2069
Fragments arranged by size:
2975 2069 1065
BlpI GC'TnA_GC
Cuts at: 2285 2591 3297 3526 2285
Size: 306 706 229 4868
Fragments arranged by size:
4868 706 306 229
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2466 4170 5326 1790
Size: 541 135 1704 1156 2573
Fragments arranged by size:
2573 1704 1156 541 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4798 442
Size: 416 53 83 399 24 2423 958 1753
Fragments arranged by size:
2423 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2548 2553 2674 2897 3311 3332 3356
Size: 850 459 5 121 223 414 21 24
Cuts at: 3356 4769 1239
Size: 1413 2579
Fragments arranged by size:
2579 1413 850 459 414 223 121 24
21 5
Bpu10I CC'TnA_GC
Cuts at: 2219 2429 2467 2219
Size: 210 38 5861
Fragments arranged by size:
5861 210 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 3154 4234 5099 5397 2462
Size: 692 1080 865 298 3174
Fragments arranged by size:
3174 1080 865 692 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2840 4772 882
Size: 617 1341 1932 2219
Fragments arranged by size:
2219 1932 1341 617
BsaAI yAC'GTr
Cuts at: 493 1204 2971 3900 493
Size: 711 1767 929 2702
Fragments arranged by size:
2702 1767 929 711
BsaBI GATnn'nnATC
Cuts at: 3215 6103 3215
Size: 2888 3221
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2016 2649 4542 4940 5214 5361 5772 2016
Size: 633 1893 398 274 147 411 2353
Fragments arranged by size:
2353 1893 633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5884
Fragments arranged by size:
5884 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 2822 2825 1186
Size: 1633 3 3 4470
Fragments arranged by size:
4470 1633 3 3
BseYI C'CCAG_C
Cuts at: 2255 2401 2439 5312 5808 2255
Size: 146 38 2873 496 2556
Fragments arranged by size:
2873 2556 496 146 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2546 1163
Size: 573 810 4726
Fragments arranged by size:
4726 810 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2426 3559 4320 5348 1540
Size: 275 186 425 1133 761 1028 2301
Fragments arranged by size:
2301 1133 1028 761 425 275 186
BsiHKAI G_wGCw'C
Cuts at: 2210 2478 2616 2913 4170 4255 5326 2210
Size: 268 138 297 1257 85 1071 2993
Fragments arranged by size:
2993 1257 1071 297 268 138 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2291 2331 2466 2478 2616 2913
Size: 399 420 81 40 135 12 138 297
Cuts at: 2913 3428 4170 4255 5326 1391
Size: 515 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 420 399 297 138
135 85 81 40 12
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 2568 2687 2928 3177 3464 844
Size: 1562 162 119 241 249 287 3489
Fragments arranged by size:
3489 1562 287 249 241 162 119
BsrBI CCG'CTC
Cuts at: 1394 1823 3286 3996 1394
Size: 429 1463 710 3507
Fragments arranged by size:
3507 1463 710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2784 4604 4778 65
Size: 1183 1536 1820 174 1396
Fragments arranged by size:
1820 1536 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 2514 4753 4940 1700
Size: 110 349 355 2239 187 2869
Fragments arranged by size:
2869 2239 355 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 2520 3005 4169 5181 2520
Size: 485 1164 1012 3448
Fragments arranged by size:
3448 1164 1012 485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2488 794
Size: 1694 4415
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2947 2947
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2046 2064 2367 2974 3129 3439 3806 4191
Size: 74 18 303 607 155 310 367 385
Cuts at: 4191 4208 5660 5746 5758 6104 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 607 385 367 346 310 303
155 86 74 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3202 487
Size: 683 56 1976 3394
Fragments arranged by size:
3394 1976 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2252 3625 4419 4439 2240
Size: 12 1373 794 20 3910
Fragments arranged by size:
3910 1373 794 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DraIII CAC_nnn'GTG
Cuts at: 2971 2971
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2361 2423
Size: 54 1425 50 275 18 327 204 62
Cuts at: 2423 2781 2835 3102 3556 4445 6074 8
Size: 358 54 267 454 889 1629 43
Fragments arranged by size:
1629 1425 889 454 358 327 275 267
204 62 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2423 3556 1537
Size: 275 611 1133 4090
Fragments arranged by size:
4090 1133 611 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4039 1353
Size: 2686 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2699 4696 5070 5216 387
Size: 1064 138 1110 1997 374 146 1280
Fragments arranged by size:
1997 1280 1110 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 2476
Size: 6109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2372 2548 2553 2674 2897 3311
Size: 393 457 283 176 5 121 223 414
Cuts at: 3311 3332 3356 4172 4769 5556 5824 1239
Size: 21 24 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 457 414 393 283
268 223 176 121 24 21 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 3068 2804
Size: 264 5845
EcoO109I rG'GnC_Cy
Cuts at: 2095 2182 2764 2917 2095
Size: 87 582 153 5287
Fragments arranged by size:
5287 582 153 87
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 6109
FspI TGC'GCA
Cuts at: 3269 4615 3269
Size: 1346 4763
HaeII r_GCGC'y
Cuts at: 1678 2440 2820 5256 1678
Size: 762 380 2436 2531
Fragments arranged by size:
2531 2436 762 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2372 2404 2909
Size: 32 135 32 680 32 88 32 505
Cuts at: 2909 2938 2941 2970 4750 4782 4824 4856 1373
Size: 29 3 29 1780 32 42 32 2626
Fragments arranged by size:
2626 1780 680 505 135 88 42 32
32 32 32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2010 748
Size: 1262 4847
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 3219 5223 5407 1305
Size: 1053 861 2004 184 2007
Fragments arranged by size:
2007 2004 1053 861 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 2837 3104 10
Size: 54 1425 343 531 474 267 3015
Fragments arranged by size:
3015 1425 531 474 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2245 2547 4067 4426 4585 518
Size: 648 1079 302 1520 359 159 2042
Fragments arranged by size:
2042 1520 1079 648 359 302 159
MspA1I CmG'CkG
Cuts at: 1606 2428 2861 2905 3256 3265 3525 4202 5350
Size: 822 433 44 351 9 260 677 1148
Cuts at: 5350 5595 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 822 677 433 351 260 245
44 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2185 2449 1348
Size: 837 264 5008
Fragments arranged by size:
5008 837 264
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2401 1108
Size: 1269 24 4816
Fragments arranged by size:
4816 1269 24
PfoI T'CCnGG_A
Cuts at: 2232 2913 2232
Size: 681 5428
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PmlI CAC'GTG
Cuts at: 2971 2971
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 2917
Size: 6109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspXI vC'TCGA_Gb
Cuts at: 2408 2408
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2178 2247 3461 830
Size: 1348 69 1214 3478
Fragments arranged by size:
3478 1348 1214 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 3256 3265 3256
Size: 9 6100
SacI G_AGCT'C
Cuts at: 2478 2478
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SexAI A'CCwGG_T
Cuts at: 2052 2052
Size: 6109
SfcI C'TryA_G
Cuts at: 826 1045 2174 2243 3140 3457 4592 5273 5464
Size: 219 1129 69 897 317 1135 681 191
Cuts at: 5464 826
Size: 1471
Fragments arranged by size:
1471 1135 1129 897 681 317 219 191
69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2477 3133 3320 4213 5114
Size: 197 926 465 69 656 187 893 901
Cuts at: 5114 5376 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 893 656 465 262 197
187 69
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 2264 1517
Size: 747 5362
StyI C'CwwG_G
Cuts at: 513 1065 2004 3099 3548 513
Size: 552 939 1095 449 3074
Fragments arranged by size:
3074 1095 939 552 449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4136 4153 4306 4491 4936 325
Size: 1255 2556 17 153 185 445 1498
Fragments arranged by size:
2556 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3114 4355 6067
Size: 274 80 33 51 1443 1137 1241 1712
Cuts at: 6067 96
Size: 138
Fragments arranged by size:
1712 1443 1241 1137 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6069 6101 6069
Size: 32 6077
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2791 2791
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 2408 1943
Size: 465 5644
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 2030 3239 4238 2030
Size: 1209 999 3901
Fragments arranged by size:
3901 1209 999
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI