Restriction map for
pFN21AE3914
(Circular) (Six-base) MAPSORT of: pFN21AE3914.seq Check: 5700 from: 1 to: 5386
pFN21AE3914 5386 bp
With 182 enzymes: SgfI *
March 28, 2012 11:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2470 3196 278
Size: 53 83 186 936 934 726 2468
Fragments arranged by size:
2468 936 934 726 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2464 2729 1937
Size: 527 265 4594
Fragments arranged by size:
4594 527 265
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3449 4833 5101 1632
Size: 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5386
AlwNI CAG_nnn'CTG
Cuts at: 3129 4701 3129
Size: 1572 3814
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2333 3940 160
Size: 872 1301 1607 1606
Fragments arranged by size:
1607 1606 1301 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2564 2711 1870
Size: 73 621 147 4545
Fragments arranged by size:
4545 621 147 73
BamHI G'GATC_C
Cuts at: 2716 3083 2716
Size: 367 5019
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2707 4162 618
Size: 325 217 627 920 1455 1842
Fragments arranged by size:
1842 1455 920 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2705 1391
Size: 1314 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2454 2488 3577 3611 1903
Size: 34 517 34 1089 34 3678
Fragments arranged by size:
3678 1089 517 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4488 3278
Size: 1210 4176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3997 136
Size: 107 122 71 631 605 2325 1525
Fragments arranged by size:
2325 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 3447 4603 1790
Size: 1657 1156 2573
Fragments arranged by size:
2573 1657 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 4075 442
Size: 416 53 83 399 24 1700 958 1753
Fragments arranged by size:
1753 1700 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 4046 1239
Size: 1277 1530 2579
Fragments arranged by size:
2579 1530 1277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 3511 4376 4674 2435
Size: 1076 865 298 3147
Fragments arranged by size:
3147 1076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 4049 882
Size: 617 687 1863 2219
Fragments arranged by size:
2219 1863 687 617
BsaAI yAC'GTr
Cuts at: 493 1204 3177 493
Size: 711 1973 2702
Fragments arranged by size:
2702 1973 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2467 3193 3575 4210
Size: 53 83 186 936 934 726 382 635
Cuts at: 4210 275
Size: 1451
Fragments arranged by size:
1451 936 934 726 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2640 3819 4217 4491 4638 5049 2640
Size: 1179 398 274 147 411 2977
Fragments arranged by size:
2977 1179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2544 2574 1178
Size: 30 165 30 1141 30 3990
Fragments arranged by size:
3990 1141 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 1186
Size: 1123 4263
BseYI C'CCAG_C
Cuts at: 2015 2039 2243 4589 5085 2015
Size: 24 204 2346 496 2316
Fragments arranged by size:
2346 2316 496 204 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 2563 1163
Size: 573 789 38 3986
Fragments arranged by size:
3986 789 573 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 3597 4625 1540
Size: 275 186 835 761 1028 2301
Fragments arranged by size:
2301 1028 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2684 3447 3532 4603 2684
Size: 763 85 1071 3467
Fragments arranged by size:
3467 1071 763 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
BsmBI CGTCTCn'nnnn_
Cuts at: 2004 2004
Size: 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2684 2705 3447 3532 4603 1391
Size: 399 894 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 894 742 399 85 21
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 2619 3275 5005 2619
Size: 656 1730 3000
Fragments arranged by size:
3000 1730 656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2741 844
Size: 1897 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2150 3273 1394
Size: 429 327 1123 3507
Fragments arranged by size:
3507 1123 429 327
BsrDI GCAATG_nn'
Cuts at: 65 1248 3881 4055 65
Size: 1183 2633 174 1396
Fragments arranged by size:
2633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4030 4217 1700
Size: 110 2220 187 2869
Fragments arranged by size:
2869 2220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5386
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5386
BstYI r'GATC_y
Cuts at: 1972 2351 2716 3083 3468 3485 4937 5023 5035
Size: 379 365 367 385 17 1452 86 12
Cuts at: 5035 5381 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 379 367 365 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2599 513
Size: 552 939 595 3300
Fragments arranged by size:
3300 939 595 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2540 487
Size: 683 56 1314 3333
Fragments arranged by size:
3333 1314 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2402 2602 2833
Size: 54 1425 50 275 18 572 200 231
Cuts at: 2833 3722 5351 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 572 275 231 200 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 1537
Size: 275 1021 4090
Fragments arranged by size:
4090 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2453 3316 1353
Size: 831 269 863 3423
Fragments arranged by size:
3423 863 831 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3973 4347 4493 387
Size: 1064 138 2384 374 146 1280
Fragments arranged by size:
2384 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2516 3449 4046 4833 5101 1239
Size: 393 884 933 597 787 268 1524
Fragments arranged by size:
1524 933 884 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FspI TGC'GCA
Cuts at: 3892 3892
Size: 5386
HaeII r_GCGC'y
Cuts at: 1678 2039 2656 4533 1678
Size: 361 617 1877 2531
Fragments arranged by size:
2531 1877 617 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2357 2389 2452
Size: 32 135 32 505 32 248 32 63
Cuts at: 2452 2484 4027 4059 4101 4133 1373
Size: 32 1543 32 42 32 2626
Fragments arranged by size:
2626 1543 505 248 135 63 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2465 2730 2976 669
Size: 988 281 527 265 246 3079
Fragments arranged by size:
3079 988 527 281 265 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2285 4500 4684 1305
Size: 980 2215 184 2007
Fragments arranged by size:
2215 2007 980 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 3344 3703 3862 518
Size: 648 1458 720 359 159 2042
Fragments arranged by size:
2042 1458 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2802 3479 4627 4872 1606
Size: 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3895
Fragments arranged by size:
3895 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2744 4289 1132
Size: 97 1515 1545 2229
Fragments arranged by size:
2229 1545 1515 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5386
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5386
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4968 5000 3455
Size: 32 1481 32 3841
Fragments arranged by size:
3841 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2738 830
Size: 1908 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2463 2728 1936
Size: 527 265 4594
Fragments arranged by size:
4594 527 265
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SfcI C'TryA_G
Cuts at: 826 1045 2734 3869 4550 4741 826
Size: 219 1689 1135 681 191 1471
Fragments arranged by size:
1689 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2713 1872
Size: 841 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 3490 4391 4653 820
Size: 197 926 471 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 471 262 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2744 1132
Size: 1612 3774
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2004 2825 513
Size: 552 939 821 3074
Fragments arranged by size:
3074 939 821 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3413 3430 3583 3768 4213 325
Size: 1255 1833 17 153 185 445 1498
Fragments arranged by size:
1833 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2156 3632 5344
Size: 274 80 33 51 1443 179 1476 1712
Cuts at: 5344 96
Size: 138
Fragments arranged by size:
1712 1476 1443 274 179 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5346 5378 5346
Size: 32 5354
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2597 2623 2497
Size: 100 26 5260
Fragments arranged by size:
5260 100 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5386
XmaI C'CCGG_G
Cuts at: 1870 2711 1870
Size: 841 4545
XmnI GAAnn'nnTTC
Cuts at: 3515 3515
Size: 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2468 3194 276
Size: 53 83 186 936 934 726 2468
Fragments arranged by size:
2468 936 934 726 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI