Restriction map for
pFN21AE3909
(Circular) (Six-base) MAPSORT of: pFN21AE3909.seq Check: 5948 from: 1 to: 5593
pFN21AE3909 5593 bp
With 182 enzymes: SgfI *
March 28, 2012 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2282 2282
Size: 5593
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2472 2936 1937
Size: 535 464 4594
Fragments arranged by size:
4594 535 464
Acc65I G'GTAC_C
Cuts at: 2914 2914
Size: 5593
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3656 5040 5308 1632
Size: 2024 1384 268 1917
Fragments arranged by size:
2024 1917 1384 268
AfeI AGC'GCT
Cuts at: 2066 2066
Size: 5593
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 4492 5469 1203
Size: 3289 977 1327
Fragments arranged by size:
3289 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 2319 3336 4908 2319
Size: 1017 1572 3004
Fragments arranged by size:
3004 1572 1017
ApaI G_GGCC'C
Cuts at: 2539 2539
Size: 5593
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2902 3128 3280 5389 1493
Size: 1409 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2918 1870
Size: 73 975 4545
Fragments arranged by size:
4545 975 73
BamHI G'GATC_C
Cuts at: 2923 3290 2923
Size: 367 5226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2595 2914 4369 618
Size: 325 217 627 808 319 1455 1842
Fragments arranged by size:
1842 1455 808 627 325 319 217
BanII G_rGCy'C
Cuts at: 1391 2115 2539 2912 1391
Size: 724 424 373 4072
Fragments arranged by size:
4072 724 424 373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2431 2465 2638 2648 2672 2682 3784
Size: 34 494 34 173 10 24 10 1102
Cuts at: 3784 3818 1903
Size: 34 3678
Fragments arranged by size:
3678 1102 494 173 34 34 34 24
10 10
BciVI GTATCCnnnnn_n'
Cuts at: 3485 4695 3485
Size: 1210 4383
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5588 5588
Size: 5593
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2539 3654 4810 1790
Size: 249 500 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 500 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3324 4282 442
Size: 416 53 83 399 24 1907 958 1753
Fragments arranged by size:
1907 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 2258
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 4253 1239
Size: 850 2164 2579
Fragments arranged by size:
2579 2164 850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3718 4583 4881 2843
Size: 875 865 298 3555
Fragments arranged by size:
3555 875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2654 4256 882
Size: 617 1155 1602 2219
Fragments arranged by size:
2219 1602 1155 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 5587 5587
Size: 5593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3400 3782 4417 275
Size: 53 83 186 936 1867 382 635 1451
Fragments arranged by size:
1867 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4026 4424 4698 4845 5256 4026
Size: 398 274 147 411 4363
Fragments arranged by size:
4363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2614 2644 1178
Size: 30 165 30 1211 30 4127
Fragments arranged by size:
4127 1211 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2197 1186
Size: 1011 4582
BseYI C'CCAG_C
Cuts at: 2077 2329 4796 5292 2077
Size: 252 2467 496 2378
Fragments arranged by size:
2467 2378 496 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2652 1163
Size: 573 916 4104
Fragments arranged by size:
4104 916 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3804 4832 1540
Size: 275 186 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 3654 3739 4810 2244
Size: 1410 85 1071 3027
Fragments arranged by size:
3027 1410 1071 85
BsmI GAATG_Cn'
Cuts at: 3104 3197 3104
Size: 93 5500
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2115 2244 2539 2912 3654 3739
Size: 399 249 76 129 295 373 742 85
Cuts at: 3739 4810 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 295 249 129
85 76
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 3482 5212 3482
Size: 1730 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2282 2437 2948 844
Size: 1438 155 511 3489
Fragments arranged by size:
3489 1438 511 155
BsrBI CCG'CTC
Cuts at: 1394 1823 3480 1394
Size: 429 1657 3507
Fragments arranged by size:
3507 1657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4088 4262 65
Size: 1183 2840 174 1396
Fragments arranged by size:
2840 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4237 4424 1700
Size: 110 2427 187 2869
Fragments arranged by size:
2869 2427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 3653 4665 3653
Size: 1012 4581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 2566
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2923 3290 3675 3692 5144 5230 5242 5588
Size: 951 367 385 17 1452 86 12 346
Cuts at: 5588 1972
Size: 1977
Fragments arranged by size:
1977 1452 951 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2154 2154
Size: 5593
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 2161 2437 2640 3109 3903 3923 2161
Size: 276 203 469 794 20 3831
Fragments arranged by size:
3831 794 469 276 203 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DraIII CAC_nnn'GTG
Cuts at: 2686 2686
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2826 3040
Size: 54 1425 50 275 18 447 549 214
Cuts at: 3040 3929 5558 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 549 447 275 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 1537
Size: 275 1228 4090
Fragments arranged by size:
4090 1228 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3523 1353
Size: 2170 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4180 4554 4700 387
Size: 1064 138 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 3656 4253 5040 5308 1239
Size: 393 457 1567 597 787 268 1524
Fragments arranged by size:
1567 1524 787 597 457 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2535 2535
Size: 5593
EcoRI G'AATT_C
Cuts at: 2902 3280 2902
Size: 378 5215
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 2068 2809 4740 1678
Size: 390 741 1931 2531
Fragments arranged by size:
2531 1931 741 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 4234 4266 4308
Size: 32 135 32 920 32 1710 32 42
Cuts at: 4308 4340 1373
Size: 32 2626
Fragments arranged by size:
2626 1710 920 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2937 3183 669
Size: 988 281 999 246 3079
Fragments arranged by size:
3079 999 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5593
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2918 2918
Size: 5593
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4707 4891 1305
Size: 3402 184 2007
Fragments arranged by size:
3402 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 2828 10
Size: 54 1425 343 447 549 2775
Fragments arranged by size:
2775 1425 549 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3551 3910 4069 518
Size: 648 2385 359 159 2042
Fragments arranged by size:
2385 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2672 3009 3686 4834 5079 1606
Size: 475 591 337 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 475 337 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5041
NdeI CA'TA_TG
Cuts at: 387 2344 387
Size: 1957 3636
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 3040 3040
Size: 5593
NspI r_CATG'y
Cuts at: 1132 1229 2050 2446 2791 2951 4496 1132
Size: 97 821 396 345 160 1545 2229
Fragments arranged by size:
2229 1545 821 396 345 160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 4492 4492
Size: 5593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2084 1108
Size: 976 4617
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PspOMI G'GGCC_C
Cuts at: 2535 2535
Size: 5593
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2213 2245 2213
Size: 32 5561
PstI C_TGCA'G
Cuts at: 830 2671 2945 830
Size: 1841 274 3478
Fragments arranged by size:
3478 1841 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
PvuII CAG'CTG
Cuts at: 2081 2672 2081
Size: 591 5002
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 3841 5553 3841
Size: 1712 3881
SfcI C'TryA_G
Cuts at: 826 1045 2667 2941 4076 4757 4948 826
Size: 219 1622 274 1135 681 191 1471
Fragments arranged by size:
1622 1471 1135 681 274 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2920 1872
Size: 1048 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 3697 4598 4860 820
Size: 197 926 915 839 901 262 1553
Fragments arranged by size:
1553 926 915 901 839 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2050 2446 2791 2951 1132
Size: 918 396 345 160 3774
Fragments arranged by size:
3774 918 396 345 160
SspI AAT'ATT
Cuts at: 5 52 3517 5
Size: 47 3465 2081
Fragments arranged by size:
3465 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 3032 513
Size: 552 1967 3074
Fragments arranged by size:
3074 1967 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 3620 3637 3790 3975 4420 325
Size: 1255 922 1118 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1118 922 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 2887 3839
Size: 274 80 33 51 1443 144 766 952
Cuts at: 3839 5551 96
Size: 1712 138
Fragments arranged by size:
1712 1443 952 766 274 144 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2614 2646 5553 5585 2614
Size: 32 2907 32 2622
Fragments arranged by size:
2907 2622 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2198 1316
Size: 882 4711
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2918 1870
Size: 1048 4545
XmnI GAAnn'nnTTC
Cuts at: 3722 3722
Size: 5593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI