Restriction map for
pFN21AE3906
(Circular) (Six-base) MAPSORT of: pFN21AE3906.seq Check: 5698 from: 1 to: 6082
pFN21AE3906 6082 bp
With 182 enzymes: SgfI *
March 28, 2012 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3175 3175
Size: 6082
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 3425 1937
Size: 922 566 4594
Fragments arranged by size:
4594 922 566
Acc65I G'GTAC_C
Cuts at: 3189 3403 3189
Size: 214 5868
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2272 2922 3028 4145 5529 5797 1632
Size: 640 650 106 1117 1384 268 1917
Fragments arranged by size:
1917 1384 1117 650 640 268 106
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 2862 2997 4981 5958 1203
Size: 1659 135 1984 977 1327
Fragments arranged by size:
1984 1659 1327 977 135
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 2656 2658 1166
Size: 1490 2 4590
Fragments arranged by size:
4590 1490 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 3340 3825 5397 3340
Size: 485 1572 4025
Fragments arranged by size:
4025 1572 485
ApaLI G'TGCA_C
Cuts at: 3027 4139 5295 3027
Size: 1112 1156 3814
Fragments arranged by size:
3814 1156 1112
ApoI r'AATT_y
Cuts at: 1493 2598 3391 3617 3769 5878 1493
Size: 1105 793 226 152 2109 1697
Fragments arranged by size:
2109 1697 1105 793 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 3407 1870
Size: 73 1464 4545
Fragments arranged by size:
4545 1464 73
BamHI G'GATC_C
Cuts at: 3115 3412 3779 3115
Size: 297 367 5418
Fragments arranged by size:
5418 367 297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3189 3403 4858 618
Size: 325 217 627 1402 214 1455 1842
Fragments arranged by size:
1842 1455 1402 627 325 217 214
BanII G_rGCy'C
Cuts at: 1391 3401 1391
Size: 2010 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3375 928
Size: 971 1476 3635
Fragments arranged by size:
3635 1476 971
BbvCI CC'TCA_GC
Cuts at: 2202 2202
Size: 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2438 3974 5184 2438
Size: 1536 1210 3336
Fragments arranged by size:
3336 1536 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4693 136
Size: 107 122 71 631 605 3021 1525
Fragments arranged by size:
3021 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3287 6077 3287
Size: 2790 3292
BlpI GC'TnA_GC
Cuts at: 3499 3499
Size: 6082
Bme1580I G_kGCm'C
Cuts at: 1790 3031 4143 5299 1790
Size: 1241 1112 1156 2573
Fragments arranged by size:
2573 1241 1156 1112
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 4771 442
Size: 416 53 83 399 24 2396 958 1753
Fragments arranged by size:
2396 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3236
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 3276 4742 1239
Size: 913 1124 1466 2579
Fragments arranged by size:
2579 1466 1124 913
Bpu10I CC'TnA_GC
Cuts at: 2202 2697 2202
Size: 495 5587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4207 5072 5370 4207
Size: 865 298 4919
Fragments arranged by size:
4919 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2693 4745 882
Size: 617 1194 2052 2219
Fragments arranged by size:
2219 2052 1194 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 3873 493
Size: 711 1236 1433 2702
Fragments arranged by size:
2702 1433 1236 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3303 3889 4271 4906
Size: 53 83 186 936 1770 586 382 635
Cuts at: 4906 275
Size: 1451
Fragments arranged by size:
1770 1451 936 635 586 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3118 3186 4515 4913 5187 5334 5745 3118
Size: 68 1329 398 274 147 411 3455
Fragments arranged by size:
3455 1329 411 398 274 147 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2867 2897 1178
Size: 30 165 30 1464 30 4363
Fragments arranged by size:
4363 1464 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6082
BseYI C'CCAG_C
Cuts at: 5285 5781 5285
Size: 496 5586
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2733 1163
Size: 573 997 4512
Fragments arranged by size:
4512 997 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3532 4293 5321 1540
Size: 275 186 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 3031 4143 4228 5299 2439
Size: 592 1112 85 1071 3222
Fragments arranged by size:
3222 1112 1071 592 85
BsmI GAATG_Cn'
Cuts at: 3250 3593 3686 3250
Size: 343 93 5646
Fragments arranged by size:
5646 343 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 3031 3401 4143 4228 5299 1391
Size: 399 649 592 370 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 592 399 370 85
BspEI T'CCGG_A
Cuts at: 3118 5745 3118
Size: 2627 3455
BspHI T'CATG_A
Cuts at: 3142 3971 5701 3142
Size: 829 1730 3523
Fragments arranged by size:
3523 1730 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3175 3437 844
Size: 2331 262 3489
Fragments arranged by size:
3489 2331 262
BsrBI CCG'CTC
Cuts at: 1394 1823 3969 1394
Size: 429 2146 3507
Fragments arranged by size:
3507 2146 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2403 2691 4577 4751 65
Size: 1183 1155 288 1886 174 1396
Fragments arranged by size:
1886 1396 1183 1155 288 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4726 4913 1700
Size: 110 2916 187 2869
Fragments arranged by size:
2916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2799 96
Size: 2703 3379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 2822 4142 5154 2822
Size: 1320 1012 3750
Fragments arranged by size:
3750 1320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6082
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstXI CCAn_nnnn'nTGG
Cuts at: 3112 3112
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 3115 3287 3412 3779 4164 4181 5633 5719
Size: 1143 172 125 367 385 17 1452 86
Cuts at: 5719 5731 6077 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 172 125
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3306 513
Size: 552 939 1302 3289
Fragments arranged by size:
3289 1302 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2747 3172 3598 4392 4412 2747
Size: 425 426 794 20 4417
Fragments arranged by size:
4417 794 426 425 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2846 3529 4418
Size: 54 1425 50 275 18 1016 683 889
Cuts at: 4418 6047 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1016 889 683 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2140 2566 4012 1353
Size: 787 426 1446 3423
Fragments arranged by size:
3423 1446 787 426
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4669 5043 5189 387
Size: 1064 138 3080 374 146 1280
Fragments arranged by size:
3080 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2272 2922 3028 3276 4145 4742
Size: 393 520 120 650 106 248 869 597
Cuts at: 4742 5529 5797 1239
Size: 787 268 1524
Fragments arranged by size:
1524 869 787 650 597 520 393 268
248 120 106
EcoO109I rG'GnC_Cy
Cuts at: 2244 2244
Size: 6082
EcoRI G'AATT_C
Cuts at: 3391 3769 3391
Size: 378 5704
EcoRV GAT'ATC
Cuts at: 3010 3010
Size: 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 6082
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 5229 1678
Size: 3551 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2525 2557 2867 2899 4723
Size: 32 135 32 953 32 310 32 1824
Cuts at: 4723 4755 4797 4829 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1824 953 310 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 3426 3672 669
Size: 988 281 618 870 246 3079
Fragments arranged by size:
3079 988 870 618 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6082
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KpnI G_GTAC'C
Cuts at: 3193 3407 3193
Size: 214 5868
MfeI C'AATT_G
Cuts at: 3128 3681 3128
Size: 553 5529
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4260
Fragments arranged by size:
4260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2656 2658 3281 4040 4399 4558 518
Size: 648 1490 2 623 759 359 159 2042
Fragments arranged by size:
2042 1490 759 648 623 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2233 2805 3498 4175 5323 5568 1606
Size: 627 572 693 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 627 572 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3306 513
Size: 552 939 1302 3289
Fragments arranged by size:
3289 1302 939 552
NdeI CA'TA_TG
Cuts at: 387 2484 3073 387
Size: 2097 589 3396
Fragments arranged by size:
3396 2097 589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 2789 2866 3001 3440 4985 1132
Size: 97 1560 77 135 439 1545 2229
Fragments arranged by size:
2229 1560 1545 439 135 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6082
PciI A'CATG_T
Cuts at: 2862 2997 4981 2862
Size: 135 1984 3963
Fragments arranged by size:
3963 1984 135
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 1108
Size: 1242 4840
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 6082
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 2244
Size: 6082
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PstI C_TGCA'G
Cuts at: 830 3434 830
Size: 2604 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 2805 2805
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SapI GCTCTTCn'nnn_
Cuts at: 2566 2566
Size: 6082
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SfcI C'TryA_G
Cuts at: 826 1045 2523 3430 4565 5246 5437 826
Size: 219 1478 907 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 907 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3409 1872
Size: 1537 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4186 5087 5349 820
Size: 197 926 2243 901 262 1553
Fragments arranged by size:
2243 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6082
StyI C'CwwG_G
Cuts at: 513 1065 2004 2388 3256 3306 3521 513
Size: 552 939 384 868 50 215 3074
Fragments arranged by size:
3074 939 868 552 384 215 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4109 4126 4279 4464 4909 325
Size: 1255 2529 17 153 185 445 1498
Fragments arranged by size:
2529 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2108 2799 3039
Size: 274 80 33 51 1443 131 691 240
Cuts at: 3039 4328 6040 96
Size: 1289 1712 138
Fragments arranged by size:
1712 1443 1289 691 274 240 138 131
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6042 6074 6042
Size: 32 6050
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6082
XbaI T'CTAG_A
Cuts at: 2885 3418 2885
Size: 533 5549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2347
Size: 6082
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 3407 1870
Size: 1537 4545
XmnI GAAnn'nnTTC
Cuts at: 2616 3138 4211 2616
Size: 522 1073 4487
Fragments arranged by size:
4487 1073 522
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI