Restriction map for
pFN21AE3905
(Circular) (Six-base) MAPSORT of: pFN21AE3905.seq Check: 4347 from: 1 to: 6040
pFN21AE3905 6040 bp
With 182 enzymes: SgfI *
March 28, 2012 11:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 2744 3166 3361 2744
Size: 422 195 5423
Fragments arranged by size:
5423 422 195
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2391 2407 2835 4103 5487 5755 1632
Size: 708 51 16 428 1268 1384 268 1917
Fragments arranged by size:
1917 1384 1268 708 428 268 51 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 3242 4939 5916 1203
Size: 2039 1697 977 1327
Fragments arranged by size:
2039 1697 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 2429 2525 3140 3349 3575 3727 5836 1493
Size: 936 96 615 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 936 615 226 209 152 96
AseI AT'TA_AT
Cuts at: 160 1032 2622 4594 160
Size: 872 1590 1972 1606
Fragments arranged by size:
1972 1606 1590 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 3365 1870
Size: 73 249 1173 4545
Fragments arranged by size:
4545 1173 249 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2744 3166 3361 4816
Size: 325 217 627 249 708 422 195 1455
Cuts at: 4816 618
Size: 1842
Fragments arranged by size:
1842 1455 708 627 422 325 249 217
195
BanII G_rGCy'C
Cuts at: 1391 3120 3359 1391
Size: 1729 239 4072
Fragments arranged by size:
4072 1729 239
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2504 3342 928
Size: 971 605 838 3626
Fragments arranged by size:
3626 971 838 605
BbvCI CC'TCA_GC
Cuts at: 2915 2915
Size: 6040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2066 3932 5142 2066
Size: 1866 1210 2964
Fragments arranged by size:
2964 1866 1210
BclI T'GATC_A
Cuts at: 1548 2895 1548
Size: 1347 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3195 6035 3195
Size: 2840 3200
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2039 4101 5257 1790
Size: 249 2062 1156 2573
Fragments arranged by size:
2573 2062 1156 249
BmgBI CAC'GTC
Cuts at: 1318 2023 1318
Size: 705 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
Bpu10I CC'TnA_GC
Cuts at: 2915 2915
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2863 3831 493
Size: 711 1659 968 2702
Fragments arranged by size:
2702 1659 968 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2139 1186
Size: 841 112 5087
Fragments arranged by size:
5087 841 112
BseYI C'CCAG_C
Cuts at: 2032 2855 5243 5739 2032
Size: 823 2388 496 2333
Fragments arranged by size:
2388 2333 823 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3120 4101 4186 5257 3120
Size: 981 85 1071 3903
Fragments arranged by size:
3903 1071 981 85
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 3120 3359 4101 4186 5257 1391
Size: 399 249 1081 239 742 85 1071 2174
Fragments arranged by size:
2174 1081 1071 742 399 249 239 85
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3395 844
Size: 2551 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3051 4535 4709 65
Size: 1183 1803 1484 174 1396
Fragments arranged by size:
1803 1484 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 4684 4871 1700
Size: 110 229 2645 187 2869
Fragments arranged by size:
2869 2645 229 187 110
BsrGI T'GTAC_A
Cuts at: 96 2782 96
Size: 2686 3354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2464 3195 3370 3737 4122 4139 5591 5677
Size: 492 731 175 367 385 17 1452 86
Cuts at: 5677 5689 6035 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 731 492 385 367 346 175
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3214 3249 529
Size: 35 2650 35 3320
Fragments arranged by size:
3320 2650 35 35
DraI TTT'AAA
Cuts at: 2173 2311 3344 2173
Size: 138 1033 4869
Fragments arranged by size:
4869 1033 138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3487 4376 6005
Size: 54 1425 50 275 18 1657 889 1629
Cuts at: 6005 8
Size: 43
Fragments arranged by size:
1657 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2206 3970 1353
Size: 853 1764 3423
Fragments arranged by size:
3423 1764 853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3118 3118
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2391 2407 2835 4103 4700 5487
Size: 393 708 51 16 428 1268 597 787
Cuts at: 5487 5755 1239
Size: 268 1524
Fragments arranged by size:
1524 1268 787 708 597 428 393 268
51 16
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
EcoRV GAT'ATC
Cuts at: 2998 2998
Size: 6040
FspI TGC'GCA
Cuts at: 3024 4546 3024
Size: 1522 4518
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2933 2965 4681
Size: 32 135 32 548 32 781 32 1716
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1716 781 548 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 3384 3630 669
Size: 988 281 289 1157 246 3079
Fragments arranged by size:
3079 1157 988 289 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 2748 3170 3365 2748
Size: 422 195 5423
Fragments arranged by size:
5423 422 195
MfeI C'AATT_G
Cuts at: 3129 3639 3129
Size: 510 5530
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4218
Fragments arranged by size:
4218 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3998 4357 4516 518
Size: 648 2832 359 159 2042
Fragments arranged by size:
2832 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 3456 4133 5281 5526 1606
Size: 657 1193 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 677 657 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5488
NdeI CA'TA_TG
Cuts at: 387 2100 3214 387
Size: 1713 1114 3213
Fragments arranged by size:
3213 1713 1114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2690 1108
Size: 1582 4458
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2263 2263
Size: 6040
SacI G_AGCT'C
Cuts at: 3120 3120
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 3057 3388 4523 5204 5395 826
Size: 219 2012 331 1135 681 191 1471
Fragments arranged by size:
2012 1471 1135 681 331 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2863 3831 493
Size: 2370 968 2702
Fragments arranged by size:
2702 2370 968
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2695 2710 3081 3479 513
Size: 552 1630 15 371 398 3074
Fragments arranged by size:
3074 1630 552 398 371 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4067 4084 4237 4422 4867 325
Size: 1255 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2782 3048 3065
Size: 274 80 33 51 1443 805 266 17
Cuts at: 3065 4286 5998 96
Size: 1221 1712 138
Fragments arranged by size:
1712 1443 1221 805 274 266 138 80
51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2245 2245
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI