Restriction map for
pFN21AE3903
(Circular) (Six-base) MAPSORT of: pFN21AE3903.seq Check: 5423 from: 1 to: 6100
pFN21AE3903 6100 bp
With 182 enzymes: SgfI *
March 28, 2012 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2200 3061 2200
Size: 861 5239
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3443 1937
Size: 1506 4594
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2582 3360 4163 5547 5815 1632
Size: 495 455 778 803 1384 268 1917
Fragments arranged by size:
1917 1384 803 778 495 455 268
AflII C'TTAA_G
Cuts at: 820 1017 3335 820
Size: 197 2318 3585
Fragments arranged by size:
3585 2318 197
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2526 1184
Size: 1342 4758
AlwNI CAG_nnn'CTG
Cuts at: 2444 3843 5415 2444
Size: 1399 1572 3129
Fragments arranged by size:
3129 1572 1399
ApaI G_GGCC'C
Cuts at: 3065 3065
Size: 6100
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3409 3635 3787 5896 1493
Size: 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 3425 1870
Size: 73 385 1097 4545
Fragments arranged by size:
4545 1097 385 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2223 2470 3421 4876 618
Size: 325 217 627 436 247 951 1455 1842
Fragments arranged by size:
1842 1455 951 627 436 325 247 217
BanII G_rGCy'C
Cuts at: 1391 3065 3419 1391
Size: 1674 354 4072
Fragments arranged by size:
4072 1674 354
BbeI G_GCGC'C
Cuts at: 2227 2227
Size: 6100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3293 3327 4291 4325 1903
Size: 34 1356 34 964 34 3678
Fragments arranged by size:
3678 1356 964 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 2419 1548
Size: 871 5229
BfrBI ATG'CAT
Cuts at: 3252 3252
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2950 4711 136
Size: 107 122 71 631 605 1278 1761 1525
Fragments arranged by size:
1761 1525 1278 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 3065 4161 5317 1790
Size: 1275 1096 1156 2573
Fragments arranged by size:
2573 1275 1156 1096
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3152 3831 4789
Size: 416 53 83 399 24 1735 679 958
Cuts at: 4789 442
Size: 1753
Fragments arranged by size:
1753 1735 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4760 1239
Size: 3521 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 2974 6094 2974
Size: 3120 2980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2224 3907 4289 4924
Size: 53 83 186 936 691 1683 382 635
Cuts at: 4924 275
Size: 1451
Fragments arranged by size:
1683 1451 936 691 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 2818 2845 2848
Size: 30 165 30 1147 30 238 27 3
Cuts at: 2848 2875 1178
Size: 27 4403
Fragments arranged by size:
4403 1147 238 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2577 2673 2697 1186
Size: 1150 241 96 24 4589
Fragments arranged by size:
4589 1150 241 96 24
BseYI C'CCAG_C
Cuts at: 2388 2979 3011 5303 5799 2388
Size: 591 32 2292 496 2689
Fragments arranged by size:
2689 2292 591 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2178 2476 1163
Size: 573 442 298 4787
Fragments arranged by size:
4787 573 442 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2762 3550 4311 5339 1540
Size: 275 186 761 788 761 1028 2301
Fragments arranged by size:
2301 1028 788 761 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4161 4246 5317 4161
Size: 85 1071 4944
Fragments arranged by size:
4944 1071 85
BsmI GAATG_Cn'
Cuts at: 2401 2639 3611 3704 2401
Size: 238 972 93 4797
Fragments arranged by size:
4797 972 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3065 3419 4161 4246 5317 1391
Size: 399 1275 354 742 85 1071 2174
Fragments arranged by size:
2174 1275 1071 742 399 354 85
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2200 2518 3061 3455 844
Size: 1356 318 543 394 3489
Fragments arranged by size:
3489 1356 543 394 318
BsrBI CCG'CTC
Cuts at: 1394 1823 3987 1394
Size: 429 2164 3507
Fragments arranged by size:
3507 2164 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4595 4769 65
Size: 1183 3347 174 1396
Fragments arranged by size:
3347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4744 4931 1700
Size: 110 2934 187 2869
Fragments arranged by size:
2934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 3159 3302 4160 5172 3159
Size: 143 858 1012 4087
Fragments arranged by size:
4087 1012 858 143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6100
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095
Size: 1458 367 385 17 1452 86 12 346
Cuts at: 6095 1972
Size: 1977
Fragments arranged by size:
1977 1458 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2111 513
Size: 552 939 107 4502
Fragments arranged by size:
4502 939 552 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 3063 3616 4410 4430 3063
Size: 553 794 20 4733
Fragments arranged by size:
4733 794 553 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065
Size: 54 1425 50 275 18 1717 889 1629
Cuts at: 6065 8
Size: 43
Fragments arranged by size:
1717 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4030 1353
Size: 2677 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2582 3360 4163 4760 5547 5815
Size: 393 495 455 778 803 597 787 268
Cuts at: 5815 1239
Size: 1524
Fragments arranged by size:
1524 803 787 778 597 495 455 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2530 3118 2530
Size: 588 5512
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2050 2227 5247 1678
Size: 372 177 3020 2531
Fragments arranged by size:
3020 2531 372 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2060 2066 2092 2108
Size: 32 135 32 462 26 6 26 16
Cuts at: 2108 2140 2234 2266 2550 2582 3251 3283 4741
Size: 32 94 32 284 32 669 32 1458
Cuts at: 4741 4773 4815 4847 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1458 669 462 284 135 94 42
32 32 32 32 32 32 32 32
26 26 16 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 3325 3444 3690 669
Size: 988 281 472 915 119 246 3079
Fragments arranged by size:
3079 988 915 472 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KasI G'GCGC_C
Cuts at: 2223 2223
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5214 5398 1305
Size: 3909 184 2007
Fragments arranged by size:
3909 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4278
Fragments arranged by size:
4278 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4058 4417 4576 518
Size: 648 2892 359 159 2042
Fragments arranged by size:
2892 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3516 4193 5341 5586 1606
Size: 1910 677 1148 245 2120
Fragments arranged by size:
2120 1910 1148 677 245
NarI GG'CG_CC
Cuts at: 2224 2224
Size: 6100
NcoI C'CATG_G
Cuts at: 513 1065 2004 2111 513
Size: 552 939 107 4502
Fragments arranged by size:
4502 939 552 107
NdeI CA'TA_TG
Cuts at: 387 2632 3025 387
Size: 2245 393 3462
Fragments arranged by size:
3462 2245 393
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NsiI A_TGCA'T
Cuts at: 3254 3254
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2607 3256 3458 5003 1132
Size: 97 1378 649 202 1545 2229
Fragments arranged by size:
2229 1545 1378 649 202 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 2530
Size: 6100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 3061 3061
Size: 6100
PspXI vC'TCGA_Gb
Cuts at: 2328 2328
Size: 6100
PstI C_TGCA'G
Cuts at: 830 3058 3452 830
Size: 2228 394 3478
Fragments arranged by size:
3478 2228 394
PvuI CG_AT'CG
Cuts at: 2001 2762 2001
Size: 761 5339
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2575 3054 3448 4583 5264 5455 826
Size: 219 1530 479 394 1135 681 191 1471
Fragments arranged by size:
1530 1471 1135 681 479 394 219 191
SfoI GGC'GCC
Cuts at: 2225 2225
Size: 6100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2328 3335 4204 5105 5367 820
Size: 197 926 385 1007 869 901 262 1553
Fragments arranged by size:
1553 1007 926 901 869 385 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2111 3539 513
Size: 552 939 107 1428 3074
Fragments arranged by size:
3074 1428 939 552 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4127 4144 4297 4482 4927 325
Size: 1255 2547 17 153 185 445 1498
Fragments arranged by size:
2547 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2519 4346 6058
Size: 274 80 33 51 1443 542 1827 1712
Cuts at: 6058 96
Size: 138
Fragments arranged by size:
1827 1712 1443 542 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2529 2546 2561 6060 6092 2514
Size: 15 17 15 3499 32 2522
Fragments arranged by size:
3499 2522 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2109 2109
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 2328 1943
Size: 385 5715
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 2808 3189 4229 2808
Size: 381 1040 4679
Fragments arranged by size:
4679 1040 381
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI