Restriction map for
pFN21AE3898
(Circular) (Six-base) MAPSORT of: pFN21AE3898.seq Check: 7082 from: 1 to: 5632
pFN21AE3898 5632 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2501 3442 278
Size: 53 83 186 936 965 941 2468
Fragments arranged by size:
2468 965 941 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2466 2975 1937
Size: 529 509 4594
Fragments arranged by size:
4594 529 509
Acc65I G'GTAC_C
Cuts at: 2526 2953 2526
Size: 427 5205
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3695 5079 5347 1632
Size: 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 2092 3375 4947 2092
Size: 1283 1572 2777
Fragments arranged by size:
2777 1572 1283
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2779 2962 3329 2779
Size: 183 367 5082
Fragments arranged by size:
5082 367 183
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2526 2953 4408 618
Size: 325 217 627 227 512 427 1455 1842
Fragments arranged by size:
1842 1455 627 512 427 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2733 2951 1391
Size: 1342 218 4072
Fragments arranged by size:
4072 1342 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2162 2346 928
Size: 971 263 184 4214
Fragments arranged by size:
4214 971 263 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 2427 2559 1548
Size: 879 132 4621
Fragments arranged by size:
4621 879 132
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 2375 3049 2375
Size: 674 4958
Bme1580I G_kGCm'C
Cuts at: 1790 2775 3693 4849 1790
Size: 985 918 1156 2573
Fragments arranged by size:
2573 1156 985 918
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2464 3363 4321
Size: 416 53 83 399 24 1047 899 958
Cuts at: 4321 442
Size: 1753
Fragments arranged by size:
1753 1047 958 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2422 2573 4292 1239
Size: 885 298 151 1719 2579
Fragments arranged by size:
2579 1719 885 298 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2335 3423 493
Size: 711 1131 1088 2702
Fragments arranged by size:
2702 1131 1088 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2498 2829 3439 3821
Size: 53 83 186 936 965 331 610 382
Cuts at: 3821 4456 275
Size: 635 1451
Fragments arranged by size:
1451 965 936 635 610 382 331 186
83 53
BsaWI w'CCGG_w
Cuts at: 2119 2523 2598 4065 4463 4737 4884 5295 2119
Size: 404 75 1467 398 274 147 411 2456
Fragments arranged by size:
2456 1467 411 404 398 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2720 2750 1178
Size: 30 165 30 1317 30 4060
Fragments arranged by size:
4060 1317 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2750 1186
Size: 838 726 4068
Fragments arranged by size:
4068 838 726
BseYI C'CCAG_C
Cuts at: 2842 4835 5331 2842
Size: 1993 496 3143
Fragments arranged by size:
3143 1993 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2520 3082 3843 4871 1540
Size: 275 186 519 562 761 1028 2301
Fragments arranged by size:
2301 1028 761 562 519 275 186
BsiHKAI G_wGCw'C
Cuts at: 2733 3693 3778 4849 2733
Size: 960 85 1071 3516
Fragments arranged by size:
3516 1071 960 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2733 2775 2951 3693 3778 4849 1391
Size: 399 943 42 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 943 742 399 176 85 42
BspEI T'CCGG_A
Cuts at: 2119 2598 5295 2119
Size: 479 2697 2456
Fragments arranged by size:
2697 2456 479
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2108 2785 3519 1394
Size: 429 285 677 734 3507
Fragments arranged by size:
3507 734 677 429 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2779 2962 3329 3714 3731 5183 5269 5281
Size: 807 183 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 807 385 367 346 183 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2233 513
Size: 552 939 229 3912
Fragments arranged by size:
3912 939 552 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 3148 3942 3962 2562
Size: 586 794 20 4232
Fragments arranged by size:
4232 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 2280 2297 2547 1554
Size: 726 17 250 4639
Fragments arranged by size:
4639 726 250 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 4639 809
Size: 507 1434 1889 1802
Fragments arranged by size:
1889 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 3079 3968
Size: 54 1425 50 275 18 702 547 889
Cuts at: 3968 5597 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 702 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 2907 3562 1353
Size: 921 633 655 3423
Fragments arranged by size:
3423 921 655 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2731 2731
Size: 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2422 2573 3695 4292 5079 5347
Size: 393 492 298 151 1122 597 787 268
Cuts at: 5347 1239
Size: 1524
Fragments arranged by size:
1524 1122 787 597 492 393 298 268
151
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 2243
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2408 2752 2408
Size: 344 5288
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2046 2223 4779 1678
Size: 368 177 2556 2531
Fragments arranged by size:
2556 2531 368 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2544 2576 2692 2724 4273
Size: 32 135 32 972 32 116 32 1549
Cuts at: 4273 4305 4347 4379 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1549 972 135 116 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2467 2976 3222 669
Size: 988 281 529 509 246 3079
Fragments arranged by size:
3079 988 529 509 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2530 2957 2530
Size: 427 5205
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 4746 4930 1305
Size: 1176 2265 184 2007
Fragments arranged by size:
2265 2007 1176 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 10
Size: 54 1425 343 702 3108
Fragments arranged by size:
3108 1425 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 2645 3590 3949 4108 518
Size: 648 1381 98 945 359 159 2042
Fragments arranged by size:
2042 1381 945 648 359 159 98
MspA1I CmG'CkG
Cuts at: 1606 2089 3048 3725 4873 5118 1606
Size: 483 959 677 1148 245 2120
Fragments arranged by size:
2120 1148 959 677 483 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2233 513
Size: 552 939 229 3912
Fragments arranged by size:
3912 939 552 229
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 1108
Size: 1638 3994
PfoI T'CCnGG_A
Cuts at: 2589 2589
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2408 2752 2408
Size: 344 5288
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SacI G_AGCT'C
Cuts at: 2733 2733
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2465 2974 1936
Size: 529 509 4594
Fragments arranged by size:
4594 529 509
SanDI GG'GwC_CC
Cuts at: 2408 2408
Size: 5632
SapI GCTCTTCn'nnn_
Cuts at: 2274 2274
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2130 2857 2980 4115 4796 4987 826
Size: 219 1085 727 123 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 727 681 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2233 3071 513
Size: 552 939 229 838 3074
Fragments arranged by size:
3074 939 838 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2506 3659 3676 3829 4014 4459 325
Size: 1255 926 1153 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1153 926 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2182 2214 2720 2752 5592 5624 2182
Size: 32 506 32 2840 32 2190
Fragments arranged by size:
2840 2190 506 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2272 2968 2272
Size: 696 4936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2644 2231
Size: 413 5219
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2499 3440 276
Size: 53 83 186 936 965 941 2468
Fragments arranged by size:
2468 965 941 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI