Restriction map for
pFN21AE3894
(Circular) (Six-base) MAPSORT of: pFN21AE3894.seq Check: 142 from: 1 to: 5632
pFN21AE3894 5632 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2650 2953 2650
Size: 303 5329
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 2622 3695 5079 5347 1632
Size: 501 489 1073 1384 268 1917
Fragments arranged by size:
1917 1384 1073 501 489 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2538 1184
Size: 1354 4278
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaI G_GGCC'C
Cuts at: 2023 2632 2840 2023
Size: 609 208 4815
Fragments arranged by size:
4815 609 208
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 2957 1870
Size: 73 462 552 4545
Fragments arranged by size:
4545 552 462 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2642 2675 2642
Size: 33 5599
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2650 2953 4408 618
Size: 325 217 627 360 503 303 1455 1842
Fragments arranged by size:
1842 1455 627 503 360 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2023 2632 2768 2840 2951 1391
Size: 632 609 136 72 111 4072
Fragments arranged by size:
4072 632 609 136 111 72
BbeI G_GCGC'C
Cuts at: 2151 2151
Size: 5632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 2814 928
Size: 971 336 579 3746
Fragments arranged by size:
3746 971 579 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4243 136
Size: 107 122 71 631 605 337 2234 1525
Fragments arranged by size:
2234 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2632 2840 3693 4849 1790
Size: 233 609 208 853 1156 2573
Fragments arranged by size:
2573 1156 853 609 233 208
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4651
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2869 2878 3363
Size: 416 53 83 399 24 1452 9 485
Cuts at: 3363 4321 442
Size: 958 1753
Fragments arranged by size:
1753 1452 958 485 416 399 83 53
24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2700 4292 1239
Size: 887 574 1592 2579
Fragments arranged by size:
2579 1592 887 574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 4295 882
Size: 617 867 1929 2219
Fragments arranged by size:
2219 1929 867 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 3439 3821 4456
Size: 53 83 186 936 615 1291 382 635
Cuts at: 4456 275
Size: 1451
Fragments arranged by size:
1451 1291 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2673 2703 1178
Size: 30 165 30 662 30 578 30 4107
Fragments arranged by size:
4107 662 578 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5632
BseYI C'CCAG_C
Cuts at: 2181 2911 4835 5331 2181
Size: 730 1924 496 2482
Fragments arranged by size:
2482 1924 730 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2136 3082 3843 4871 1540
Size: 275 186 15 120 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186 120 15
BsiHKAI G_wGCw'C
Cuts at: 3693 3778 4849 3693
Size: 85 1071 4476
Fragments arranged by size:
4476 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2632 2768 2840 2951 3693 3778
Size: 399 233 609 136 72 111 742 85
Cuts at: 3778 4849 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 609 399 233 136 111
85 72
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2743 3519 1394
Size: 429 920 776 3507
Fragments arranged by size:
3507 920 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2170 4276 4463 1700
Size: 110 360 2106 187 2869
Fragments arranged by size:
2869 2106 360 187 110
BsrGI T'GTAC_A
Cuts at: 96 2667 96
Size: 2571 3061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 2154 3692 4704 2154
Size: 1538 1012 3082
Fragments arranged by size:
3082 1538 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2189 2455 2189
Size: 266 5366
BstYI r'GATC_y
Cuts at: 1972 2343 2962 3329 3714 3731 5183 5269 5281
Size: 371 619 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 619 385 371 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 2760 2841 513
Size: 552 939 114 642 81 3304
Fragments arranged by size:
3304 939 642 552 114 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2704 2936 2704
Size: 232 5400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2133 3079
Size: 54 1425 50 275 18 183 120 946
Cuts at: 3079 3968 5597 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 946 889 275 183 120 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2133 3079 1537
Size: 275 201 120 946 4090
Fragments arranged by size:
4090 946 275 201 120
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 3562 1353
Size: 1176 1033 3423
Fragments arranged by size:
3423 1176 1033
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2133 2622 2700 3695 4292 5079
Size: 393 494 7 489 78 995 597 787
Cuts at: 5079 5347 1239
Size: 268 1524
Fragments arranged by size:
1524 995 787 597 494 489 393 268
78 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2796 2796
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2194 2628 2837 2019
Size: 1 174 434 209 4814
Fragments arranged by size:
4814 434 209 174 1
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 2514 4138 2514
Size: 1624 4008
HaeII r_GCGC'y
Cuts at: 1678 2151 2641 4779 1678
Size: 473 490 2138 2531
Fragments arranged by size:
2531 2138 490 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 4273 4305 4347
Size: 32 135 32 772 32 1897 32 42
Cuts at: 4347 4379 1373
Size: 32 2626
Fragments arranged by size:
2626 1897 772 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 2976 3222 669
Size: 988 281 160 878 246 3079
Fragments arranged by size:
3079 988 878 281 246 160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KasI G'GCGC_C
Cuts at: 2147 2147
Size: 5632
KpnI G_GTAC'C
Cuts at: 2654 2957 2654
Size: 303 5329
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 2900 4746 4930 1305
Size: 1260 335 1846 184 2007
Fragments arranged by size:
2007 1846 1260 335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3590 3949 4108 518
Size: 648 2424 359 159 2042
Fragments arranged by size:
2424 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2656 2722 3048 3725 4873 5118 1606
Size: 514 536 66 326 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 514 326 245 66
NaeI GCC'GGC
Cuts at: 2172 2172
Size: 5632
NarI GG'CG_CC
Cuts at: 2148 2148
Size: 5632
NcoI C'CATG_G
Cuts at: 513 1065 2004 2760 513
Size: 552 939 756 3385
Fragments arranged by size:
3385 939 756 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NgoMIV G'CCGG_C
Cuts at: 2170 2170
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2668 2990 4535 1132
Size: 97 1439 322 1545 2229
Fragments arranged by size:
2229 1545 1439 322 97
PasI CC'CwG_GG
Cuts at: 1348 2189 1348
Size: 841 4791
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2821 1108
Size: 1713 3919
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2194
Size: 5632
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2019 2628 2836 2019
Size: 609 208 4815
Fragments arranged by size:
4815 609 208
PstI C_TGCA'G
Cuts at: 830 2169 2561 2984 830
Size: 1339 392 423 3478
Fragments arranged by size:
3478 1339 423 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2722 2722
Size: 5632
SacII CC_GC'GG
Cuts at: 2121 2121
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SanDI GG'GwC_CC
Cuts at: 2194 2194
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2165 2557 2980 4115 4796 4987 826
Size: 219 1120 392 423 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 423 392 219 191
SfoI GGC'GCC
Cuts at: 2149 2149
Size: 5632
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 1517
Size: 693 4939
StyI C'CwwG_G
Cuts at: 513 1065 2004 2760 3071 513
Size: 552 939 756 311 3074
Fragments arranged by size:
3074 939 756 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2203 2515 2851 3659 3676 3829 4014
Size: 1255 623 312 336 808 17 153 185
Cuts at: 4014 4459 325
Size: 445 1498
Fragments arranged by size:
1498 1255 808 623 445 336 312 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 2865 3878
Size: 274 80 33 51 1443 690 198 1013
Cuts at: 3878 5590 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1013 690 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2673 2705 5592 5624 2673
Size: 32 2887 32 2681
Fragments arranged by size:
2887 2681 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 2726 2776 2455
Size: 271 50 5311
Fragments arranged by size:
5311 271 50
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI
SwaI