Restriction map for
pFN21AE3882
(Circular) (Six-base) MAPSORT of: pFN21AE3882.seq Check: 4500 from: 1 to: 6151
pFN21AE3882 6151 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 3494 1937
Size: 475 1082 4594
Fragments arranged by size:
4594 1082 475
Acc65I G'GTAC_C
Cuts at: 3221 3472 3221
Size: 251 5900
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2837 3273 4214 5598 5866 1632
Size: 1205 436 941 1384 268 1917
Fragments arranged by size:
1917 1384 1205 941 436 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 2078 5050 6027 1203
Size: 875 2972 977 1327
Fragments arranged by size:
2972 1327 977 875
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 2181
Size: 32 6119
AlwNI CAG_nnn'CTG
Cuts at: 3894 5466 3894
Size: 1572 4579
ApaI G_GGCC'C
Cuts at: 3271 3271
Size: 6151
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 3460 3686 3838 5947 1493
Size: 1967 226 152 2109 1697
Fragments arranged by size:
2109 1967 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3221 3472 4927 618
Size: 325 217 627 1434 251 1455 1842
Fragments arranged by size:
1842 1455 1434 627 325 251 217
BanII G_rGCy'C
Cuts at: 1391 3149 3271 3470 1391
Size: 1758 122 199 4072
Fragments arranged by size:
4072 1758 199 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3043 4043 5253 3043
Size: 1000 1210 3941
Fragments arranged by size:
3941 1210 1000
BclI T'GATC_A
Cuts at: 1548 3355 1548
Size: 1807 4344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3266 4762 136
Size: 107 122 71 631 605 1594 1496 1525
Fragments arranged by size:
1594 1525 1496 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3093 3442 6146 3093
Size: 349 2704 3098
Fragments arranged by size:
3098 2704 349
BlpI GC'TnA_GC
Cuts at: 3161 3568 3161
Size: 407 5744
Bme1580I G_kGCm'C
Cuts at: 1790 3271 4212 5368 1790
Size: 1481 941 1156 2573
Fragments arranged by size:
2573 1481 1156 941
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 3119 4811 1239
Size: 814 1066 1692 2579
Fragments arranged by size:
2579 1692 1066 814
Bpu10I CC'TnA_GC
Cuts at: 2584 2584
Size: 6151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4276 5141 5439 4276
Size: 865 298 4988
Fragments arranged by size:
4988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2737 4584 4982 5256 5403 5814 2737
Size: 1847 398 274 147 411 3074
Fragments arranged by size:
3074 1847 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2262 2292 1178
Size: 30 165 30 859 30 5037
Fragments arranged by size:
5037 859 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6151
BseYI C'CCAG_C
Cuts at: 3424 5354 5850 3424
Size: 1930 496 3725
Fragments arranged by size:
3725 1930 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5578
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 2047 3662 3755 2047
Size: 1615 93 4443
Fragments arranged by size:
4443 1615 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3149 3271 3470 4212 4297 5368 1391
Size: 399 1359 122 199 742 85 1071 2174
Fragments arranged by size:
2174 1359 1071 742 399 199 122 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 2476 4040 5770 2476
Size: 1564 1730 2857
Fragments arranged by size:
2857 1730 1564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3506 844
Size: 2662 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2553 2796 2892 3051 4646 4820 65
Size: 1183 1305 243 96 159 1595 174 1396
Fragments arranged by size:
1595 1396 1305 1183 243 174 159 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 2904 4211 5223 2904
Size: 1307 1012 3832
Fragments arranged by size:
3832 1307 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2528 794
Size: 1734 4417
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 3093 3442 3481 3848 4233 4250 5702 5788
Size: 1121 349 39 367 385 17 1452 86
Cuts at: 5788 5800 6146 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1121 385 367 349 346 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 513
Size: 552 939 181 4479
Fragments arranged by size:
4479 939 552 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3097 487
Size: 683 56 1871 3541
Fragments arranged by size:
3541 1871 683 56
BtsI GCAGTG_nn'
Cuts at: 2475 3667 4461 4481 2475
Size: 1192 794 20 4145
Fragments arranged by size:
4145 1192 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 2433 3455 2433
Size: 1022 5129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116
Size: 54 1425 50 275 18 1768 889 1629
Cuts at: 6116 8
Size: 43
Fragments arranged by size:
1768 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4081 1353
Size: 2728 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2837 3119 3273 4214 4811 5598
Size: 393 421 784 282 154 941 597 787
Cuts at: 5598 5866 1239
Size: 268 1524
Fragments arranged by size:
1524 941 787 784 597 421 393 282
268 154
EcoNI CCTnn'n_nnAGG
Cuts at: 2035 2035
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 3267 3268 3267
Size: 1 6150
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
EcoRV GAT'ATC
Cuts at: 2617 2617
Size: 6151
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 4792 4824 4866
Size: 32 135 32 692 32 2496 32 42
Cuts at: 4866 4898 1373
Size: 32 2626
Fragments arranged by size:
2626 2496 692 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2319 2427 748
Size: 1571 108 4472
Fragments arranged by size:
4472 1571 108
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 3225 3476 3225
Size: 251 5900
MfeI C'AATT_G
Cuts at: 3213 3750 3213
Size: 537 5614
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3225 5265 5449 1305
Size: 1920 2040 184 2007
Fragments arranged by size:
2040 2007 1920 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4109 4468 4627 518
Size: 648 2943 359 159 2042
Fragments arranged by size:
2943 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3353 3567 4244 5392 5637 1606
Size: 1747 214 677 1148 245 2120
Fragments arranged by size:
2120 1747 1148 677 245 214
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2082 3509 5054 1132
Size: 97 853 1427 1545 2229
Fragments arranged by size:
2229 1545 1427 853 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 2078 5050 2078
Size: 2972 3179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3142 3402 1108
Size: 2034 260 3857
Fragments arranged by size:
3857 2034 260
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3178 3210 4220 4252 5733 5765 3178
Size: 32 1010 32 1481 32 3564
Fragments arranged by size:
3564 1481 1010 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 2373 3721 2373
Size: 1348 4803
PspOMI G'GGCC_C
Cuts at: 3267 3267
Size: 6151
PstI C_TGCA'G
Cuts at: 830 2014 2470 2533 2812 3503 830
Size: 1184 456 63 279 691 3478
Fragments arranged by size:
3478 1184 691 456 279 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 3353 3353
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2010 2466 2529 2808 2963 3499 4634
Size: 219 965 456 63 279 155 536 1135
Cuts at: 4634 5315 5506 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 965 681 536 456 279 219
191 155 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4255 5156 5418 820
Size: 197 926 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 2336 2722 152
Size: 2184 386 3581
Fragments arranged by size:
3581 2184 386
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 2560 4075 5
Size: 47 2508 1515 2081
Fragments arranged by size:
2508 2081 1515 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 2952 3271 3590 513
Size: 552 939 948 319 319 3074
Fragments arranged by size:
3074 948 939 552 319 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 4178 4195 4348 4533 4978 325
Size: 1255 1100 1498 17 153 185 445 1498
Fragments arranged by size:
1498 1498 1255 1100 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4397 6109 96
Size: 274 80 33 51 1443 2420 1712 138
Fragments arranged by size:
2420 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 2262 2294 6111 6143 2145
Size: 32 85 32 3817 32 2153
Fragments arranged by size:
3817 2153 85 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2803 1316
Size: 1487 4664
XbaI T'CTAG_A
Cuts at: 2377 3487 2377
Size: 1110 5041
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI