Restriction map for
pFN21AE3875
(Circular) (Six-base) MAPSORT of: pFN21AE3875.seq Check: 6226 from: 1 to: 5761
pFN21AE3875 5761 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2790 2924 3003 3104 1937
Size: 853 134 79 101 4594
Fragments arranged by size:
4594 853 134 101 79
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2507 2921 3824 5208 5476 1632
Size: 713 162 414 903 1384 268 1917
Fragments arranged by size:
1917 1384 903 713 414 268 162
AfeI AGC'GCT
Cuts at: 2016 2016
Size: 5761
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2265 2470 4660 5637 1203
Size: 1062 205 2190 977 1327
Fragments arranged by size:
2190 1327 1062 977 205
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2046 3504 5076 2046
Size: 1458 1572 2731
Fragments arranged by size:
2731 1572 1458
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2711 2954 3070 3296 3448 5557 1493
Size: 1218 243 116 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 243 226 152 116
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 2075 3091 3458 2075
Size: 1016 367 4378
Fragments arranged by size:
4378 1016 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2642 3082 4537 618
Size: 325 217 627 855 440 1455 1842
Fragments arranged by size:
1842 1455 855 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 2358 2568 3080 1391
Size: 826 141 210 512 4072
Fragments arranged by size:
4072 826 512 210 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2495 928
Size: 971 596 4194
Fragments arranged by size:
4194 971 596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2465 4372 136
Size: 107 122 71 631 605 793 1907 1525
Fragments arranged by size:
1907 1525 793 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 2376 3822 4978 1790
Size: 586 1446 1156 2573
Fragments arranged by size:
2573 1446 1156 586
BmgBI CAC'GTC
Cuts at: 1318 2473 1318
Size: 1155 4606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 3492 4450
Size: 416 53 83 399 24 697 1378 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1378 958 697 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 4421 1239
Size: 992 2190 2579
Fragments arranged by size:
2579 2190 992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2268 2371 3552 493
Size: 711 1064 103 1181 2702
Fragments arranged by size:
2702 1181 1064 711 103
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 1178
Size: 30 165 30 1203 30 4303
Fragments arranged by size:
4303 1203 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 2774 1186
Size: 1546 42 4173
Fragments arranged by size:
4173 1546 42
BseYI C'CCAG_C
Cuts at: 2751 4964 5460 2751
Size: 2213 496 3052
Fragments arranged by size:
3052 2213 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2371 1163
Size: 573 483 152 4553
Fragments arranged by size:
4553 573 483 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2358 2982 3822 3907 4978 2217
Size: 141 624 840 85 1071 3000
Fragments arranged by size:
3000 1071 840 624 141 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 2479 2479
Size: 5761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2358 2376 2568 2982 3080 3822
Size: 399 427 141 18 192 414 98 742
Cuts at: 3822 3907 4978 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 414 399 192 141
98 85 18
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 2406 3650 5380 2406
Size: 1244 1730 2787
Fragments arranged by size:
2787 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 2351 2828 3821 4833 2351
Size: 477 993 1012 3279
Fragments arranged by size:
3279 1012 993 477
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 4313
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 2071 2071
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2075 2451 3091 3458 3843 3860 5312 5398
Size: 103 376 640 367 385 17 1452 86
Cuts at: 5398 5410 5756 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 640 385 376 367 346 103
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3829
Fragments arranged by size:
3829 1380 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 3277 4071 4091 2223
Size: 1054 794 20 3893
Fragments arranged by size:
3893 1054 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DraIII CAC_nnn'GTG
Cuts at: 2335 2335
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2457 3208
Size: 54 1425 50 275 18 231 396 751
Cuts at: 3208 4097 5726 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 751 396 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2847 3691 1353
Size: 1494 844 3423
Fragments arranged by size:
3423 1494 844
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2467 2575 4348 4722 4868 387
Size: 1064 138 878 108 1773 374 146 1280
Fragments arranged by size:
1773 1280 1064 878 374 146 138 108
EcoICRI GAG'CTC
Cuts at: 2215 2356 2215
Size: 141 5620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2345 2507 2921 3824 4421 5208
Size: 393 599 114 162 414 903 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 903 787 599 597 414 393 268
162 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2572 2572
Size: 5761
EcoO109I rG'GnC_Cy
Cuts at: 2169 2686 2169
Size: 517 5244
EcoRI G'AATT_C
Cuts at: 2954 3070 3448 2954
Size: 116 378 5267
Fragments arranged by size:
5267 378 116
FspI TGC'GCA
Cuts at: 2693 4267 2693
Size: 1574 4187
HaeII r_GCGC'y
Cuts at: 1678 2018 2286 4908 1678
Size: 340 268 2622 2531
Fragments arranged by size:
2622 2531 340 268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 4402 4434 4476
Size: 32 135 32 495 32 2303 32 42
Cuts at: 4476 4508 1373
Size: 32 2626
Fragments arranged by size:
2626 2303 495 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 10
Size: 54 1425 343 231 3708
Fragments arranged by size:
3708 1425 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3719 4078 4237 518
Size: 648 2553 359 159 2042
Fragments arranged by size:
2553 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 2665 2755 2878 2992 3177 3854 5002
Size: 603 456 90 123 114 185 677 1148
Cuts at: 5002 5247 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 603 456 245 185 123
114 90
NcoI C'CATG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3829
Fragments arranged by size:
3829 1380 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PmlI CAC'GTG
Cuts at: 2268 2268
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 3830 3862 5343 5375 2957
Size: 32 841 32 1481 32 3343
Fragments arranged by size:
3343 1481 841 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2169 2169
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2187 2877 3113 830
Size: 1357 690 236 3478
Fragments arranged by size:
3478 1357 690 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2209 2878 2992 2209
Size: 669 114 4978
Fragments arranged by size:
4978 669 114
SacI G_AGCT'C
Cuts at: 2217 2358 2217
Size: 141 5620
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SanDI GG'GwC_CC
Cuts at: 2169 2169
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 2187 3113 2187
Size: 926 4835
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SexAI A'CCwGG_T
Cuts at: 2253 2253
Size: 5761
SfcI C'TryA_G
Cuts at: 826 1045 2183 2873 3109 4244 4925 5116 826
Size: 219 1138 690 236 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 690 681 236 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2376 2445 3200 513
Size: 552 1311 69 755 3074
Fragments arranged by size:
3074 1311 755 552 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2133 2621 2693 2765 3788 3805 3958
Size: 1255 553 488 72 72 1023 17 153
Cuts at: 3958 4143 4588 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1023 553 488 445 185 153
72 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 2502 2613
Size: 274 80 33 51 1443 456 69 111
Cuts at: 2613 4007 5719 96
Size: 1394 1712 138
Fragments arranged by size:
1712 1443 1394 456 274 138 111 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 2705 1316
Size: 1389 4372
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SfoI SrfI SwaI XcmI