Restriction map for
pFN21AE3874
(Circular) (Six-base) MAPSORT of: pFN21AE3874.seq Check: 799 from: 1 to: 6802
pFN21AE3874 6802 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 6802
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4612 278
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3903 4145 1937
Size: 1966 242 4594
Fragments arranged by size:
4594 1966 242
Acc65I G'GTAC_C
Cuts at: 4123 4123
Size: 6802
AclI AA'CG_TT
Cuts at: 1563 4929 5302 1563
Size: 3366 373 3063
Fragments arranged by size:
3366 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2781 3207 4865 6249 6517 1632
Size: 1149 426 1658 1384 268 1917
Fragments arranged by size:
1917 1658 1384 1149 426 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6605
AflIII A'CryG_T
Cuts at: 1203 5701 6678 1203
Size: 4498 977 1327
Fragments arranged by size:
4498 1327 977
AgeI A'CCGG_T
Cuts at: 3612 5633 3612
Size: 2021 4781
AhdI GACnn_n'nnGTC
Cuts at: 5531 5531
Size: 6802
AleI CACnn'nnGTG
Cuts at: 1166 2523 1166
Size: 1357 5445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2690 3233 1184
Size: 1506 543 4753
Fragments arranged by size:
4753 1506 543
AlwNI CAG_nnn'CTG
Cuts at: 2804 4545 6117 2804
Size: 1741 1572 3489
Fragments arranged by size:
3489 1741 1572
ApaI G_GGCC'C
Cuts at: 2142 2697 2142
Size: 555 6247
ApaLI G'TGCA_C
Cuts at: 4859 6015 4859
Size: 1156 5646
ApoI r'AATT_y
Cuts at: 1493 2314 2780 3537 3892 4111 4337 4489 6598
Size: 821 466 757 355 219 226 152 2109
Cuts at: 6598 1493
Size: 1697
Fragments arranged by size:
2109 1697 821 757 466 355 226 219
152
AseI AT'TA_AT
Cuts at: 160 1032 5356 160
Size: 872 4324 1606
Fragments arranged by size:
4324 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 2214 2444 2538 3411 4127 1870
Size: 73 265 6 230 94 873 716 4545
Fragments arranged by size:
4545 873 716 265 230 94 73 6
AvrII C'CTAG_G
Cuts at: 3383 3383
Size: 6802
BamHI G'GATC_C
Cuts at: 4132 4499 4132
Size: 367 6435
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3113 4123 5578 618
Size: 325 217 627 1326 1010 1455 1842
Fragments arranged by size:
1842 1455 1326 1010 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 2215 2697 2704 3971 4121 1391
Size: 751 73 482 7 1267 150 4072
Fragments arranged by size:
4072 1267 751 482 150 73 7
BbeI G_GCGC'C
Cuts at: 3117 3117
Size: 6802
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3802 4054 928
Size: 971 1903 252 3676
Fragments arranged by size:
3676 1903 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4993 5027 1903
Size: 34 3056 34 3678
Fragments arranged by size:
3678 3056 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4694 5904 4694
Size: 1210 5592
BclI T'GATC_A
Cuts at: 1548 3520 1548
Size: 1972 4830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5413 136
Size: 107 122 71 631 605 3741 1525
Fragments arranged by size:
3741 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6797 6797
Size: 6802
BlpI GC'TnA_GC
Cuts at: 4219 4219
Size: 6802
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2697 3212 4863 6019 1790
Size: 352 555 515 1651 1156 2573
Fragments arranged by size:
2573 1651 1156 555 515 352
BmgBI CAC'GTC
Cuts at: 1318 2635 1318
Size: 1317 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4533 5491 442
Size: 416 53 83 399 24 3116 958 1753
Fragments arranged by size:
3116 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 6802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2879 3938 5462 1239
Size: 830 810 1059 1524 2579
Fragments arranged by size:
2579 1524 1059 830 810
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4927 5792 6090 4927
Size: 865 298 5639
Fragments arranged by size:
5639 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 2236 5465 882
Size: 617 657 80 3229 2219
Fragments arranged by size:
3229 2219 657 617 80
BsaAI yAC'GTr
Cuts at: 493 1204 2898 4593 493
Size: 711 1694 1695 2702
Fragments arranged by size:
2702 1695 1694 711
BsaBI GATnn'nnATC
Cuts at: 6796 6796
Size: 6802
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3114 3766 4609 4991
Size: 53 83 186 936 1581 652 843 382
Cuts at: 4991 5626 275
Size: 635 1451
Fragments arranged by size:
1581 1451 936 843 652 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2568 3612 5235 5633 5907 6054 6465 2568
Size: 1044 1623 398 274 147 411 2905
Fragments arranged by size:
2905 1623 1044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6577
Fragments arranged by size:
6577 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2408 1186
Size: 1219 3 5580
Fragments arranged by size:
5580 1219 3
BseYI C'CCAG_C
Cuts at: 2281 6005 6501 2281
Size: 3724 496 2582
Fragments arranged by size:
3724 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2803 4097 1163
Size: 573 1067 1294 3868
Fragments arranged by size:
3868 1294 1067 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 2192 3669 4252 5013 6041
Size: 275 186 145 46 1477 583 761 1028
Cuts at: 6041 1540
Size: 2301
Fragments arranged by size:
2301 1477 1028 761 583 275 186 145
46
BsiHKAI G_wGCw'C
Cuts at: 2415 2619 2704 3657 4863 4948 6019 2415
Size: 204 85 953 1206 85 1071 3198
Fragments arranged by size:
3198 1206 1071 953 204 85 85
BsmI GAATG_Cn'
Cuts at: 4313 4406 4313
Size: 93 6709
BsmBI CGTCTCn'nnnn_
Cuts at: 3656 3656
Size: 6802
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2215 2415 2619 2697 2704 3212
Size: 399 352 73 200 204 78 7 508
Cuts at: 3212 3657 3971 4121 4863 4948 6019 1391
Size: 445 314 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 445 399 352 314
204 200 150 85 78 73 7
BspEI T'CCGG_A
Cuts at: 6465 6465
Size: 6802
BspHI T'CATG_A
Cuts at: 2332 2607 3523 4691 6421 2332
Size: 275 916 1168 1730 2713
Fragments arranged by size:
2713 1730 1168 916 275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 2265 3157 3412 4157 844
Size: 1275 146 892 255 745 3489
Fragments arranged by size:
3489 1275 892 745 255 146
BsrBI CCG'CTC
Cuts at: 1394 1823 2311 3159 4689 1394
Size: 429 488 848 1530 3507
Fragments arranged by size:
3507 1530 848 488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5297 5471 65
Size: 1183 4049 174 1396
Fragments arranged by size:
4049 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 2286 3612 5446 5633 1700
Size: 110 355 121 1326 1834 187 2869
Fragments arranged by size:
2869 1834 1326 355 187 121 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6802
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6802
BssSI C'ACGA_G
Cuts at: 4862 5874 4862
Size: 1012 5790
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 3446 794
Size: 1441 1211 4150
Fragments arranged by size:
4150 1441 1211
BstBI TT'CG_AA
Cuts at: 5606 5606
Size: 6802
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6802
BstXI CCAn_nnnn'nTGG
Cuts at: 3428 3428
Size: 6802
BstYI r'GATC_y
Cuts at: 1972 3332 4132 4499 4884 4901 6353 6439 6451
Size: 1360 800 367 385 17 1452 86 12
Cuts at: 6451 6797 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1360 800 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2932 2932
Size: 6802
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 513
Size: 552 939 49 5262
Fragments arranged by size:
5262 939 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6063
Fragments arranged by size:
6063 683 56
BtsI GCAGTG_nn'
Cuts at: 2415 4318 5112 5132 2415
Size: 1903 794 20 4085
Fragments arranged by size:
4085 1903 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6767
DraI TTT'AAA
Cuts at: 4106 4106
Size: 6802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5809 809
Size: 507 4493 1802
Fragments arranged by size:
4493 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2199 2601
Size: 54 1425 50 275 18 303 66 402
Cuts at: 2601 3309 4249 5138 6767 8
Size: 708 940 889 1629 43
Fragments arranged by size:
1629 1425 940 889 708 402 303 275
66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4249 1537
Size: 275 2437 4090
Fragments arranged by size:
4090 2437 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2343 3077 3166 3867 3913 4082 4732 1353
Size: 990 734 89 701 46 169 650 3423
Fragments arranged by size:
3423 990 734 701 650 169 89 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5389 5763 5909 387
Size: 1064 138 3800 374 146 1280
Fragments arranged by size:
3800 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2702 2702
Size: 6802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2781 2879 3207 3938 4865 5462
Size: 393 437 712 98 328 731 927 597
Cuts at: 5462 6249 6517 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 787 731 712 597 437 393
328 268 98
EcoNI CCTnn'n_nnAGG
Cuts at: 2931 3940 2931
Size: 1009 5793
EcoO109I rG'GnC_Cy
Cuts at: 2693 2814 3016 2693
Size: 121 202 6479
Fragments arranged by size:
6479 202 121
EcoRI G'AATT_C
Cuts at: 4111 4489 4111
Size: 378 6424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2448 2801 2448
Size: 353 6449
FspI TGC'GCA
Cuts at: 5308 5308
Size: 6802
HaeII r_GCGC'y
Cuts at: 1678 3117 5949 1678
Size: 1439 2832 2531
Fragments arranged by size:
2832 2531 1439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 2505 2537 2740
Size: 32 135 32 630 32 271 32 203
Cuts at: 2740 2772 2899 2931 3467 3499 3512 3544 5443
Size: 32 127 32 536 32 13 32 1899
Cuts at: 5443 5475 5517 5549 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1899 630 536 271 203 135 127
42 32 32 32 32 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3472 3904 4146 4392 669
Size: 988 281 1534 432 242 246 3079
Fragments arranged by size:
3079 1534 988 432 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6802
HpaI GTT'AAC
Cuts at: 3472 4392 3472
Size: 920 5882
KasI G'GCGC_C
Cuts at: 3113 3113
Size: 6802
KpnI G_GTAC'C
Cuts at: 4127 4127
Size: 6802
MfeI C'AATT_G
Cuts at: 4401 4401
Size: 6802
MluI A'CGCG_T
Cuts at: 6678 6678
Size: 6802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 3787 5916 6100 1305
Size: 834 1648 2129 184 2007
Fragments arranged by size:
2129 2007 1648 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2603 3311 10
Size: 54 1425 343 369 402 708 3501
Fragments arranged by size:
3501 1425 708 402 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 4760 5119 5278 518
Size: 648 1357 2237 359 159 2042
Fragments arranged by size:
2237 2042 1357 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3360 4218 4895 6043 6288 1606
Size: 1754 858 677 1148 245 2120
Fragments arranged by size:
2120 1754 1148 858 677 245
NarI GG'CG_CC
Cuts at: 3114 3114
Size: 6802
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5311
Fragments arranged by size:
5311 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6802
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6802
NotI GC'GGCC_GC
Cuts at: 4249 4249
Size: 6802
NspI r_CATG'y
Cuts at: 1132 1229 2677 4160 5705 1132
Size: 97 1448 1483 1545 2229
Fragments arranged by size:
2229 1545 1483 1448 97
PasI CC'CwG_GG
Cuts at: 1348 2690 1348
Size: 1342 5460
PciI A'CATG_T
Cuts at: 5701 5701
Size: 6802
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3822 1108
Size: 2714 4088
PfoI T'CCnGG_A
Cuts at: 2085 2649 3095 2085
Size: 564 446 5792
Fragments arranged by size:
5792 564 446
PmeI GTTT'AAAC
Cuts at: 4106 4106
Size: 6802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4871 4903 6384 6416 4871
Size: 32 1481 32 5257
Fragments arranged by size:
5257 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2814 3016 2814
Size: 202 6600
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6802
PsiI TTA'TAA
Cuts at: 2463 4372 2463
Size: 1909 4893
PspOMI G'GGCC_C
Cuts at: 2138 2693 2138
Size: 555 6247
PspXI vC'TCGA_Gb
Cuts at: 2208 2208
Size: 6802
PstI C_TGCA'G
Cuts at: 830 2130 3587 4154 830
Size: 1300 1457 567 3478
Fragments arranged by size:
3478 1457 1300 567
PvuI CG_AT'CG
Cuts at: 2001 2192 3669 2001
Size: 191 1477 5134
Fragments arranged by size:
5134 1477 191
SacI G_AGCT'C
Cuts at: 2704 2704
Size: 6802
SalI G'TCGA_C
Cuts at: 1936 3902 4144 1936
Size: 1966 242 4594
Fragments arranged by size:
4594 1966 242
SapI GCTCTTCn'nnn_
Cuts at: 3077 3166 3077
Size: 89 6713
SbfI CC_TGCA'GG
Cuts at: 4154 4154
Size: 6802
ScaI AGT'ACT
Cuts at: 2234 5050 6762 2234
Size: 2816 1712 2274
Fragments arranged by size:
2816 2274 1712
SfcI C'TryA_G
Cuts at: 826 1045 2126 3232 3583 3708 4150 5285 5966
Size: 219 1081 1106 351 125 442 1135 681
Cuts at: 5966 6157 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1106 1081 681 442 351 219
191 125
SfoI GGC'GCC
Cuts at: 3115 3115
Size: 6802
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6802
SmaI CCC'GGG
Cuts at: 1872 2216 4129 1872
Size: 344 1913 4545
Fragments arranged by size:
4545 1913 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2208 4906 5807 6069 820
Size: 197 926 265 2698 901 262 1553
Fragments arranged by size:
2698 1553 926 901 265 262 197
SnaBI TAC'GTA
Cuts at: 493 4593 493
Size: 4100 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6802
SphI G_CATG'C
Cuts at: 1132 4160 1132
Size: 3028 3774
SspI AAT'ATT
Cuts at: 5 52 2327 4726 5
Size: 47 2275 2399 2081
Fragments arranged by size:
2399 2275 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6802
StyI C'CwwG_G
Cuts at: 513 1065 2004 2943 3109 3383 3994 4241 513
Size: 552 939 939 166 274 611 247 3074
Fragments arranged by size:
3074 939 939 611 552 274 247 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4829 4846 4999 5184 5629 325
Size: 1255 3249 17 153 185 445 1498
Fragments arranged by size:
3249 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 5048 6760
Size: 274 80 33 51 1443 255 2816 1712
Cuts at: 6760 96
Size: 138
Fragments arranged by size:
2816 1712 1443 274 255 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2812 2837 2844 2869 6762 6794 2812
Size: 25 7 25 3893 32 2820
Fragments arranged by size:
3893 2820 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 2636 1316
Size: 1320 5482
XbaI T'CTAG_A
Cuts at: 4138 4138
Size: 6802
XhoI C'TCGA_G
Cuts at: 1943 2208 1943
Size: 265 6537
XmaI C'CCGG_G
Cuts at: 1870 2214 4127 1870
Size: 344 1913 4545
Fragments arranged by size:
4545 1913 344
XmnI GAAnn'nnTTC
Cuts at: 4931 4931
Size: 6802
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4610 276
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI XcmI