Restriction map for
pFN21AE3872
(Circular) (Six-base) MAPSORT of: pFN21AE3872.seq Check: 577 from: 1 to: 5260
pFN21AE3872 5260 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3070 278
Size: 53 83 186 936 1534 2468
Fragments arranged by size:
2468 1534 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2603 1937
Size: 666 4594
Acc65I G'GTAC_C
Cuts at: 2581 2581
Size: 5260
AclI AA'CG_TT
Cuts at: 1563 3387 3760 1563
Size: 1824 373 3063
Fragments arranged by size:
3063 1824 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3323 4707 4975 1632
Size: 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1384 268
AfeI AGC'GCT
Cuts at: 2285 2285
Size: 5260
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5063
AflIII A'CryG_T
Cuts at: 1203 4159 5136 1203
Size: 2956 977 1327
Fragments arranged by size:
2956 1327 977
AgeI A'CCGG_T
Cuts at: 4091 4091
Size: 5260
AhdI GACnn_n'nnGTC
Cuts at: 3989 3989
Size: 5260
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5260
AlwNI CAG_nnn'CTG
Cuts at: 2362 3003 4575 2362
Size: 641 1572 3047
Fragments arranged by size:
3047 1572 641
ApaLI G'TGCA_C
Cuts at: 2347 3317 4473 2347
Size: 970 1156 3134
Fragments arranged by size:
3134 1156 970
ApoI r'AATT_y
Cuts at: 1493 2569 2795 2947 5056 1493
Size: 1076 226 152 2109 1697
Fragments arranged by size:
2109 1697 1076 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3814 160
Size: 872 2782 1606
Fragments arranged by size:
2782 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2479 2533 2585 1870
Size: 73 525 11 54 52 4545
Fragments arranged by size:
4545 525 73 54 52 11
AvrII C'CTAG_G
Cuts at: 2053 2053
Size: 5260
BamHI G'GATC_C
Cuts at: 2590 2957 2590
Size: 367 4893
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2451 2581 4036 618
Size: 325 217 627 441 223 130 1455 1842
Fragments arranged by size:
1842 1455 627 441 325 223 217 130
BanII G_rGCy'C
Cuts at: 1391 2119 2172 2524 2579 1391
Size: 728 53 352 55 4072
Fragments arranged by size:
4072 728 352 55 53
BbeI G_GCGC'C
Cuts at: 2455 2455
Size: 5260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 2490 928
Size: 971 354 237 3698
Fragments arranged by size:
3698 971 354 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3451 3485 1903
Size: 34 1514 34 3678
Fragments arranged by size:
3678 1514 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3152 4362 3152
Size: 1210 4050
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2436 3871 136
Size: 107 122 71 631 605 764 1435 1525
Fragments arranged by size:
1525 1435 764 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2373 5255 2373
Size: 2882 2378
BlpI GC'TnA_GC
Cuts at: 2677 2677
Size: 5260
Bme1580I G_kGCm'C
Cuts at: 1790 2351 3321 4477 1790
Size: 561 970 1156 2573
Fragments arranged by size:
2573 1156 970 561
BmgBI CAC'GTC
Cuts at: 1318 2179 2346 1318
Size: 861 167 4232
Fragments arranged by size:
4232 861 167
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 3949 442
Size: 416 53 83 399 24 1574 958 1753
Fragments arranged by size:
1753 1574 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2508 3920 1239
Size: 1269 1412 2579
Fragments arranged by size:
2579 1412 1269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3385 4250 4548 3385
Size: 865 298 4097
Fragments arranged by size:
4097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3923 882
Size: 617 2424 2219
Fragments arranged by size:
2424 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3051 493
Size: 711 1847 2702
Fragments arranged by size:
2702 1847 711
BsaBI GATnn'nnATC
Cuts at: 5254 5254
Size: 5260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2452 3067 3449 4084
Size: 53 83 186 936 919 615 382 635
Cuts at: 4084 275
Size: 1451
Fragments arranged by size:
1451 936 919 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2257 2319 3693 4091 4365 4512 4923 2257
Size: 62 1374 398 274 147 411 2594
Fragments arranged by size:
2594 1374 411 398 274 147 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5035
Fragments arranged by size:
5035 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5260
BseYI C'CCAG_C
Cuts at: 4463 4959 4463
Size: 496 4764
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 4048
Fragments arranged by size:
4048 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2446 2710 3471 4499 1540
Size: 275 186 21 424 264 761 1028 2301
Fragments arranged by size:
2301 1028 761 424 275 264 186 21
BsiHKAI G_wGCw'C
Cuts at: 2351 2548 3321 3406 4477 2351
Size: 197 773 85 1071 3134
Fragments arranged by size:
3134 1071 773 197 85
BsmI GAATG_Cn'
Cuts at: 2771 2864 2771
Size: 93 5167
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2172 2351 2524 2548 2579 3321
Size: 399 329 53 179 173 24 31 742
Cuts at: 3321 3406 4477 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 329 179 173 85
53 31 24
BspEI T'CCGG_A
Cuts at: 2257 2319 4923 2257
Size: 62 2604 2594
Fragments arranged by size:
2604 2594 62
BspHI T'CATG_A
Cuts at: 3149 4879 3149
Size: 1730 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2615 844
Size: 1771 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2069 3147 1394
Size: 429 246 1078 3507
Fragments arranged by size:
3507 1078 429 246
BsrDI GCAATG_nn'
Cuts at: 65 1248 3755 3929 65
Size: 1183 2507 174 1396
Fragments arranged by size:
2507 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2448 2454 2474 3904 4091 1700
Size: 110 638 6 20 1430 187 2869
Fragments arranged by size:
2869 1430 638 187 110 20 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5260
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5260
BssSI C'ACGA_G
Cuts at: 3320 4332 3320
Size: 1012 4248
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5260
BstBI TT'CG_AA
Cuts at: 2095 4064 2095
Size: 1969 3291
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5260
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5260
BstYI r'GATC_y
Cuts at: 1972 2373 2590 2957 3342 3359 4811 4897 4909
Size: 401 217 367 385 17 1452 86 12
Cuts at: 4909 5255 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 401 385 367 346 217 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4708
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4521
Fragments arranged by size:
4521 683 56
BtsI GCAGTG_nn'
Cuts at: 2776 3570 3590 2776
Size: 794 20 4446
Fragments arranged by size:
4446 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5225
DraI TTT'AAA
Cuts at: 2564 2564
Size: 5260
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2494 4267 809
Size: 507 1178 1773 1802
Fragments arranged by size:
1802 1773 1178 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 2707 3596
Size: 54 1425 50 275 18 613 264 889
Cuts at: 3596 5225 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 613 275 264 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2443 2707 1537
Size: 275 631 264 4090
Fragments arranged by size:
4090 631 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 3190 1353
Size: 773 1064 3423
Fragments arranged by size:
3423 1064 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3847 4221 4367 387
Size: 1064 138 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 3323 3920 4707 4975 1239
Size: 393 876 815 597 787 268 1524
Fragments arranged by size:
1524 876 815 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 2468
Size: 5260
EcoO109I rG'GnC_Cy
Cuts at: 2314 2314
Size: 5260
EcoRI G'AATT_C
Cuts at: 2569 2947 2569
Size: 378 4882
FseI GG_CCGG'CC
Cuts at: 2478 2478
Size: 5260
FspI TGC'GCA
Cuts at: 3766 3766
Size: 5260
HaeII r_GCGC'y
Cuts at: 1678 2061 2287 2455 2461 4407 1678
Size: 383 226 168 6 1946 2531
Fragments arranged by size:
2531 1946 383 226 168 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 3901 3933 3975
Size: 32 135 32 497 32 1800 32 42
Cuts at: 3975 4007 1373
Size: 32 2626
Fragments arranged by size:
2626 1800 497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 2850 669
Size: 988 281 666 246 3079
Fragments arranged by size:
3079 988 666 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5260
HpaI GTT'AAC
Cuts at: 2850 2850
Size: 5260
KasI G'GCGC_C
Cuts at: 2451 2451
Size: 5260
KpnI G_GTAC'C
Cuts at: 2585 2585
Size: 5260
MfeI C'AATT_G
Cuts at: 2859 2859
Size: 5260
MluI A'CGCG_T
Cuts at: 5136 5136
Size: 5260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 2067 2465 4374 4558 1305
Size: 750 12 398 1909 184 2007
Fragments arranged by size:
2007 1909 750 398 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3438
Fragments arranged by size:
3438 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3218 3577 3736 518
Size: 648 2052 359 159 2042
Fragments arranged by size:
2052 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2676 3353 4501 4746 1606
Size: 1070 677 1148 245 2120
Fragments arranged by size:
2120 1148 1070 677 245
NaeI GCC'GGC
Cuts at: 2450 2456 2476 2450
Size: 6 20 5234
Fragments arranged by size:
5234 20 6
NarI GG'CG_CC
Cuts at: 2452 2452
Size: 5260
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4708
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5260
NgoMIV G'CCGG_C
Cuts at: 2448 2454 2474 2448
Size: 6 20 5234
Fragments arranged by size:
5234 20 6
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5260
NotI GC'GGCC_GC
Cuts at: 2707 2707
Size: 5260
NspI r_CATG'y
Cuts at: 1132 1229 2618 4163 1132
Size: 97 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 97
PasI CC'CwG_GG
Cuts at: 1348 2434 1348
Size: 1086 4174
PciI A'CATG_T
Cuts at: 4159 4159
Size: 5260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5260
PmeI GTTT'AAAC
Cuts at: 2564 2564
Size: 5260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 4842 4874 3329
Size: 32 1481 32 3715
Fragments arranged by size:
3715 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2253 1613
Size: 640 4620
PsiI TTA'TAA
Cuts at: 2830 2830
Size: 5260
PstI C_TGCA'G
Cuts at: 830 2612 830
Size: 1782 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5260
SalI G'TCGA_C
Cuts at: 1936 2602 1936
Size: 666 4594
SbfI CC_TGCA'GG
Cuts at: 2612 2612
Size: 5260
ScaI AGT'ACT
Cuts at: 3508 5220 3508
Size: 1712 3548
SfcI C'TryA_G
Cuts at: 826 1045 2608 3743 4424 4615 826
Size: 219 1563 1135 681 191 1471
Fragments arranged by size:
1563 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2453 2453
Size: 5260
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
SgrAI Cr'CCGG_yG
Cuts at: 1700 2448 2454 1700
Size: 748 6 4506
Fragments arranged by size:
4506 748 6
SmaI CCC'GGG
Cuts at: 1872 2481 2587 1872
Size: 609 106 4545
Fragments arranged by size:
4545 609 106
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3364 4265 4527 820
Size: 197 926 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3051 493
Size: 2558 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5260
SphI G_CATG'C
Cuts at: 1132 2618 1132
Size: 1486 3774
SspI AAT'ATT
Cuts at: 5 52 3184 5
Size: 47 3132 2081
Fragments arranged by size:
3132 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5260
StyI C'CwwG_G
Cuts at: 513 1065 2053 2699 513
Size: 552 988 646 3074
Fragments arranged by size:
3074 988 646 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 3287 3304 3457 3642 4087 325
Size: 1255 428 1279 17 153 185 445 1498
Fragments arranged by size:
1498 1279 1255 445 428 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3506 5218 96
Size: 274 80 33 51 1443 1529 1712 138
Fragments arranged by size:
1712 1529 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5220 5252 5220
Size: 32 5228
Tth111I GACn'n_nGTC
Cuts at: 1316 2405 1316
Size: 1089 4171
XbaI T'CTAG_A
Cuts at: 2596 2596
Size: 5260
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5260
XmaI C'CCGG_G
Cuts at: 1870 2479 2585 1870
Size: 609 106 4545
Fragments arranged by size:
4545 609 106
XmnI GAAnn'nnTTC
Cuts at: 2306 3389 2306
Size: 1083 4177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3068 276
Size: 53 83 186 936 1534 2468
Fragments arranged by size:
2468 1534 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI